FastQCFastQC Report
Sat 18 Jun 2016
SRR3553239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556347
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT31940.5741021340997614Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12840.23079121483534554No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTC11640.20922194242082728No Hit
AAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT9100.1635669824767636No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC9070.16302775066640063No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC8280.14882797966017614No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6790.12204613307881591No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT6230.11198047261870739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG453.8380676E-1045.0000041
TAAACCG453.8380676E-1045.0000041
CGTTCAT453.8380676E-1045.00000417
AGGGCGC453.8380676E-1045.0000046
ACGCGAG207.0301443E-445.01
TCGCATA207.0301443E-445.033
CGAGTGT351.2104101E-745.035
GCGAAAT207.0301443E-445.035
CGCATAA207.0301443E-445.034
ATTACGG351.2104101E-745.02
TTGTCGC207.0301443E-445.021
GGATCGG207.0301443E-445.08
ATTCGGT207.0301443E-445.037
TACGGGT551.8189894E-1245.04
TCGATAA207.0301443E-445.028
TTTCACG207.0301443E-445.01
ATATCGG207.0301443E-445.02
ACGATAG406.8048394E-945.01
CTTGCGT551.8189894E-1245.025
CGCTAGC207.0301443E-445.039