##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556347
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883072974240896	33.0	31.0	34.0	30.0	34.0
2	32.074333105058535	33.0	31.0	34.0	30.0	34.0
3	32.227794883409096	34.0	31.0	34.0	30.0	34.0
4	35.88771755756749	37.0	35.0	37.0	35.0	37.0
5	35.68096170195939	37.0	35.0	37.0	33.0	37.0
6	35.70026080845228	37.0	35.0	37.0	33.0	37.0
7	35.8062701874909	37.0	35.0	37.0	35.0	37.0
8	35.83376382006194	37.0	35.0	37.0	35.0	37.0
9	37.520331735409734	39.0	37.0	39.0	35.0	39.0
10	37.09658720187221	39.0	37.0	39.0	33.0	39.0
11	37.10646772607743	39.0	37.0	39.0	34.0	39.0
12	37.04218590196406	39.0	37.0	39.0	33.0	39.0
13	36.99057782283359	39.0	37.0	39.0	33.0	39.0
14	38.180617492320444	40.0	37.0	41.0	33.0	41.0
15	38.17964507762242	40.0	37.0	41.0	33.0	41.0
16	38.10361339236124	40.0	37.0	41.0	33.0	41.0
17	38.05516880651823	40.0	37.0	41.0	33.0	41.0
18	38.01329386156481	40.0	37.0	41.0	33.0	41.0
19	38.07376511421828	40.0	37.0	41.0	33.0	41.0
20	37.9423561194722	40.0	37.0	41.0	33.0	41.0
21	37.93807102401918	40.0	37.0	41.0	33.0	41.0
22	37.91039764751135	40.0	37.0	41.0	33.0	41.0
23	37.75346321630206	40.0	37.0	41.0	33.0	41.0
24	37.64871563969968	39.0	36.0	41.0	33.0	41.0
25	37.66832750064258	40.0	36.0	41.0	33.0	41.0
26	37.63122116233214	40.0	36.0	41.0	33.0	41.0
27	37.56460985679801	40.0	36.0	41.0	33.0	41.0
28	37.45993597520972	39.0	36.0	41.0	32.0	41.0
29	37.29591783545162	39.0	36.0	41.0	32.0	41.0
30	37.30614886033357	39.0	36.0	41.0	32.0	41.0
31	37.24930843520321	39.0	36.0	41.0	32.0	41.0
32	37.18550652740107	39.0	36.0	41.0	32.0	41.0
33	37.10743474845735	39.0	35.0	41.0	31.0	41.0
34	37.02994713730819	39.0	35.0	41.0	31.0	41.0
35	36.89623562273186	39.0	35.0	41.0	31.0	41.0
36	36.86071462594388	39.0	35.0	41.0	31.0	41.0
37	36.718474261566975	39.0	35.0	41.0	31.0	41.0
38	36.6901376299324	39.0	35.0	41.0	31.0	41.0
39	36.64684450531772	39.0	35.0	41.0	31.0	41.0
40	36.57350538422963	39.0	35.0	40.0	30.0	41.0
41	36.43818875629778	39.0	35.0	40.0	30.0	41.0
42	36.35101833927387	39.0	35.0	40.0	30.0	41.0
43	36.251758345061624	38.0	35.0	40.0	30.0	41.0
44	36.198737478588	38.0	35.0	40.0	30.0	41.0
45	36.0879990365725	38.0	35.0	40.0	30.0	41.0
46	35.99858182033874	38.0	35.0	40.0	30.0	41.0
47	35.85936115409987	38.0	35.0	40.0	29.0	41.0
48	35.76747964849276	38.0	35.0	40.0	29.0	41.0
49	35.55549324432413	38.0	34.0	40.0	28.0	41.0
50	35.518918948066585	38.0	34.0	40.0	28.0	41.0
51	34.23482287133749	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	6.0
12	9.0
13	6.0
14	11.0
15	28.0
16	50.0
17	91.0
18	192.0
19	390.0
20	679.0
21	1054.0
22	1522.0
23	2005.0
24	2371.0
25	2940.0
26	3509.0
27	4390.0
28	5485.0
29	7113.0
30	9247.0
31	12058.0
32	16141.0
33	22241.0
34	34787.0
35	42976.0
36	51318.0
37	75429.0
38	117837.0
39	142409.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.962361619636663	31.127515741075264	21.24806999947874	27.662052639809325
2	22.455050535007828	30.24407429176395	21.93109695927182	25.369778213956394
3	21.469334785664344	30.133711514576333	20.50950216321828	27.887451536541043
4	20.734721316013207	31.490418749449532	18.79132987146511	28.98353006307215
5	18.425191472228665	33.02579145748966	17.832575712639773	30.716441357641905
6	20.191355395104136	36.39832694343638	20.852992826419484	22.557324835040003
7	84.93530116995328	5.168896390202518	5.70237639458827	4.193426045255928
8	85.93305976306155	4.690777518347362	4.692934445588815	4.683228273002281
9	78.55798629272738	8.076254567742794	8.395120311604089	4.970638827925737
10	35.39104192167838	34.48279580909037	13.507397361718496	16.618764907512755
11	26.535058156150747	28.71858750024715	25.57612425338862	19.170230090213483
12	26.90389271443901	22.391421181385	31.049327128572635	19.655358975603356
13	27.10682361907227	23.719549130308963	27.506214646614435	21.667412604004337
14	22.48614623607209	26.85787826662137	28.160123088647914	22.495852408658624
15	20.281047619561175	28.824456679015075	29.322886615727235	21.571609085696515
16	22.70777051013127	27.218983835627764	27.3392325293387	22.734013124902262
17	21.734277348489343	27.10376797214688	28.48474063848641	22.677214040877367
18	23.312069625611354	26.07185803104897	26.364660904076054	24.251411439263627
19	23.690430612549363	29.30491222204847	25.683611127587643	21.32104603781453
20	24.853913115375835	27.014255491626628	26.091989351969186	22.03984204102835
21	25.228858967514878	27.113653888670203	25.709314510548275	21.94817263326665
22	22.99769748016975	25.829383460322425	26.813122026361246	24.35979703314658
23	23.905224617010607	27.178721193787332	24.770691672643153	24.14536251655891
24	21.3870120626156	27.333480723361497	26.475562913074036	24.803944300948867
25	21.916897188265597	27.95449602496284	25.124966972051617	25.003639814719953
26	24.088922920407587	27.067279952978986	25.563901665687062	23.279895460926365
27	21.90826947929979	26.399890715686432	26.87800958754159	24.813830217472187
28	21.952306743812763	27.203166369190452	28.810975883756	22.033551003240785
29	24.85804722592195	27.366733351667214	25.285658051539777	22.48956137087106
30	23.895338700487287	25.384517216772984	27.92411929964572	22.796024783094005
31	25.142581877856806	28.19858829112047	23.70822526229134	22.950604568731386
32	25.441675788671457	25.767551546067473	26.37544554028331	22.415327124977757
33	24.662485822696986	26.560581795174592	26.469451619223257	22.307480762905165
34	24.18526567052577	25.45677427936162	27.793625201537893	22.56433484857472
35	25.419207796573	24.860923128910553	27.443304268738757	22.27656480577769
36	25.314237337489015	27.08687204208884	27.480870751527377	20.118019868894773
37	25.004538534403885	27.499024889142927	25.959518070556687	21.536918505896498
38	25.033836796100275	26.609292402044048	26.695030259891755	21.66184054196392
39	25.65826723250058	24.57099615887207	26.1369253361661	23.63381127246125
40	26.581791579715535	24.158124336070834	26.72558672914566	22.53449735506797
41	22.454331559260677	23.793064400455112	30.08895527431621	23.663648765968002
42	24.868472374255635	25.416871125394763	26.956557687917794	22.75809881243181
43	23.56478960073479	24.783633236091866	27.76594463527259	23.885632527900754
44	23.88509329609039	23.295173695553313	27.36188026537395	25.457852742982347
45	24.885548048250463	23.93326467114948	28.18834288672357	22.99284439387648
46	23.766821785684115	24.16135972693301	29.5301313748434	22.541687112539478
47	23.928411584856214	24.39952044317665	28.15419153873392	23.517876433233216
48	23.491094586651855	23.68665598987682	30.361267338549503	22.46098208492182
49	23.164140365635117	24.363212167945544	28.484560894549627	23.988086571869715
50	22.97648769562881	22.79764247852509	28.034302332896555	26.191567492949545
51	23.086131497069275	22.146789683417005	29.920355461609393	24.84672335790433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1628.0
1	1367.5
2	1107.0
3	773.5
4	440.0
5	399.5
6	359.0
7	397.0
8	435.0
9	496.5
10	558.0
11	631.0
12	704.0
13	860.0
14	1016.0
15	957.5
16	899.0
17	975.5
18	1052.0
19	1025.5
20	999.0
21	1136.5
22	1274.0
23	1816.5
24	2359.0
25	2765.5
26	3943.0
27	4714.0
28	5922.5
29	7131.0
30	8670.0
31	10209.0
32	11032.5
33	11856.0
34	13423.0
35	14990.0
36	16845.0
37	18700.0
38	19420.0
39	20140.0
40	21045.0
41	21950.0
42	23623.5
43	25297.0
44	29973.5
45	34650.0
46	38840.0
47	43030.0
48	47853.5
49	52677.0
50	50183.5
51	47690.0
52	43876.5
53	40063.0
54	36652.5
55	33242.0
56	31186.5
57	29131.0
58	27744.0
59	26357.0
60	25424.0
61	24491.0
62	22339.0
63	20187.0
64	17893.5
65	15600.0
66	13717.5
67	11835.0
68	9835.5
69	7836.0
70	7171.0
71	6506.0
72	5665.0
73	4824.0
74	3993.5
75	2571.5
76	1980.0
77	1535.5
78	1091.0
79	813.0
80	535.0
81	359.0
82	183.0
83	159.5
84	136.0
85	106.5
86	77.0
87	65.0
88	53.0
89	33.0
90	13.0
91	7.5
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.546184281283736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.54491533193666	20.054292439155674
2	10.278090367065978	5.456881618876412
3	3.520390758625918	2.8035882546183557
4	1.6950034626371735	1.7998349710632175
5	1.0076859598787875	1.337510859430229
6	0.6381577424531379	1.0164391819013403
7	0.49799721286840026	0.9253948049059167
8	0.3828793700999739	0.8131189052940595
9	0.31383297648927444	0.7497961224685259
>10	4.443805588655318	29.78832780652518
>50	1.5162697245670227	27.403572471250303
>100	0.15551484354533568	6.114991902600451
>500	0.0034104132356433258	0.7146711192574908
>1k	0.0020462479413859954	1.021579542652841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	3194	0.5741021340997614	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1284	0.23079121483534554	No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTC	1164	0.20922194242082728	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	910	0.1635669824767636	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	907	0.16302775066640063	No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	828	0.14882797966017614	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	679	0.12204613307881591	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	623	0.11198047261870739	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6522907466023903	0.0
2	0.0	0.0	0.0	0.9887713962688753	0.0
3	0.0	0.0	0.0	1.3320823155332913	0.0
4	0.0	0.0	0.0	2.866736047826267	0.0
5	0.0	0.0	0.0	3.10076265352379	0.0
6	0.0	0.0	0.0	4.319246801007285	0.0
7	0.0	0.0	0.0	5.155954826753806	0.0
8	0.0	0.0	0.0	5.783980141889864	0.0
9	0.0	0.0	0.0	6.676049300166982	0.0
10	0.0	0.0	0.0	8.10483385369203	0.0
11	0.0	0.0	0.0	9.31379157252578	0.0
12	0.0	0.0	0.0	10.102148479276423	0.0
13	0.0	0.0	0.0	10.388300826642364	0.0
14	0.0	0.0	0.0	10.693685775244587	0.0
15	0.0	0.0	0.0	10.949641141230204	0.0
16	0.0	0.0	0.0	11.537403814525826	0.0
17	0.0	0.0	0.0	12.209106906301283	0.0
18	0.0	0.0	0.0	13.277145378693513	0.0
19	0.0	0.0	0.0	13.636633252268817	0.0
20	0.0	0.0	0.0	14.137759348032793	0.0
21	0.0	0.0	0.0	14.619113610750125	0.0
22	1.797439367876523E-4	0.0	0.0	15.133181269962812	0.0
23	1.797439367876523E-4	0.0	0.0	15.645810977681196	0.0
24	1.797439367876523E-4	0.0	0.0	16.018779646515576	0.0
25	1.797439367876523E-4	0.0	0.0	16.329017681411063	0.0
26	1.797439367876523E-4	0.0	0.0	16.634043142139706	0.0
27	1.797439367876523E-4	0.0	0.0	17.034153145429023	0.0
28	1.797439367876523E-4	0.0	0.0	17.402088984033345	0.0
29	1.797439367876523E-4	0.0	0.0	17.74180502456201	0.0
30	1.797439367876523E-4	0.0	0.0	18.26791552753947	0.0
31	1.797439367876523E-4	0.0	0.0	18.59073563801009	0.0
32	1.797439367876523E-4	0.0	0.0	18.941236314746014	0.0
33	1.797439367876523E-4	0.0	0.0	19.270527206940994	1.797439367876523E-4
34	1.797439367876523E-4	0.0	0.0	19.581124729710055	1.797439367876523E-4
35	1.797439367876523E-4	0.0	0.0	19.928390015583798	1.797439367876523E-4
36	1.797439367876523E-4	0.0	0.0	20.27457683783682	1.797439367876523E-4
37	1.797439367876523E-4	0.0	0.0	20.617887757101233	1.797439367876523E-4
38	1.797439367876523E-4	0.0	0.0	20.96497329903819	1.797439367876523E-4
39	1.797439367876523E-4	0.0	0.0	21.316552439394837	1.797439367876523E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACACG	45	3.8380676E-10	45.000004	1
TAAACCG	45	3.8380676E-10	45.000004	1
CGTTCAT	45	3.8380676E-10	45.000004	17
AGGGCGC	45	3.8380676E-10	45.000004	6
ACGCGAG	20	7.0301443E-4	45.0	1
TCGCATA	20	7.0301443E-4	45.0	33
CGAGTGT	35	1.2104101E-7	45.0	35
GCGAAAT	20	7.0301443E-4	45.0	35
CGCATAA	20	7.0301443E-4	45.0	34
ATTACGG	35	1.2104101E-7	45.0	2
TTGTCGC	20	7.0301443E-4	45.0	21
GGATCGG	20	7.0301443E-4	45.0	8
ATTCGGT	20	7.0301443E-4	45.0	37
TACGGGT	55	1.8189894E-12	45.0	4
TCGATAA	20	7.0301443E-4	45.0	28
TTTCACG	20	7.0301443E-4	45.0	1
ATATCGG	20	7.0301443E-4	45.0	2
ACGATAG	40	6.8048394E-9	45.0	1
CTTGCGT	55	1.8189894E-12	45.0	25
CGCTAGC	20	7.0301443E-4	45.0	39
>>END_MODULE