FastQCFastQC Report
Sat 18 Jun 2016
SRR3553238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399066
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGT44641.1186119589240877No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC42461.063984403582365No Hit
AATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT38100.9547292928989191No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT36210.9073687059283427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17300.43351225110633324No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT10770.26988017019741095No Hit
AAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT9310.23329474322543137No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC8140.20397628462459844No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTTC8060.20197160369462694No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTAT7890.19771165671843755No Hit
AACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG7880.19746107160219115No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT7130.17866718788370844No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT7120.17841660276746202No Hit
ACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG4630.1160209088220996No Hit
TACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT4310.10800218510221367No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.10649867440473507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATA406.7957444E-945.013
CGGGTAT453.8380676E-1045.06
AATCACG207.027746E-445.01
CGAGTCA207.027746E-445.01
GCGGTCG207.027746E-445.012
CGTGATA207.027746E-445.02
ATTACGG351.2092642E-745.02
CACGCGC207.027746E-445.02
GATTTCG351.2092642E-745.05
TTGTAGG453.8380676E-1045.02
TAAGTCG207.027746E-445.01
TTCCGCG207.027746E-445.01
TACGGAT351.2092642E-745.04
TTATCCG207.027746E-445.01
TCTAGCG453.8380676E-1045.01
TCGGCAA207.027746E-445.024
ATCGTGC207.027746E-445.011
CTGCGTT207.027746E-445.039
CATTAGT253.8862134E-545.026
TAGTTCG351.2092642E-745.01