##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399066
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66822530608972	31.0	31.0	34.0	30.0	34.0
2	31.87595284990453	33.0	31.0	34.0	30.0	34.0
3	32.036948775390535	33.0	31.0	34.0	30.0	34.0
4	35.70257300797362	37.0	35.0	37.0	33.0	37.0
5	35.456586128610304	37.0	35.0	37.0	33.0	37.0
6	34.79094184921792	37.0	35.0	37.0	32.0	37.0
7	35.20483328572216	37.0	35.0	37.0	33.0	37.0
8	35.66693479274105	37.0	35.0	37.0	33.0	37.0
9	37.37188835931901	39.0	37.0	39.0	34.0	39.0
10	36.86577909418492	39.0	37.0	39.0	32.0	39.0
11	36.864724130845524	39.0	37.0	39.0	32.0	39.0
12	36.88328246455473	39.0	37.0	39.0	33.0	39.0
13	36.90194604401277	39.0	37.0	39.0	33.0	39.0
14	38.0371517493347	40.0	37.0	41.0	33.0	41.0
15	38.07764630412012	40.0	37.0	41.0	33.0	41.0
16	37.91282143805787	40.0	37.0	41.0	33.0	41.0
17	37.910423338495384	40.0	37.0	41.0	33.0	41.0
18	37.84292573158325	40.0	37.0	41.0	33.0	41.0
19	37.84182566292292	40.0	37.0	41.0	33.0	41.0
20	37.848839540326665	40.0	37.0	41.0	33.0	41.0
21	37.859629735432236	39.0	37.0	41.0	33.0	41.0
22	37.839600467090655	39.0	37.0	41.0	33.0	41.0
23	37.71290463231646	39.0	37.0	41.0	33.0	41.0
24	37.59786852300121	39.0	36.0	41.0	32.0	41.0
25	37.631183814206175	39.0	36.0	41.0	32.0	41.0
26	37.55759448311808	39.0	36.0	41.0	32.0	41.0
27	37.456027323801074	39.0	36.0	41.0	32.0	41.0
28	37.38457798960573	39.0	36.0	41.0	32.0	41.0
29	37.232973994276634	39.0	36.0	41.0	31.0	41.0
30	37.23024261650955	39.0	36.0	41.0	31.0	41.0
31	37.18146371778102	39.0	36.0	41.0	31.0	41.0
32	37.115677607212845	39.0	36.0	41.0	31.0	41.0
33	36.89880370665504	39.0	36.0	41.0	31.0	41.0
34	36.94862754531832	39.0	36.0	41.0	31.0	41.0
35	36.82105967433958	39.0	35.0	41.0	30.0	41.0
36	36.88299178581989	39.0	36.0	41.0	31.0	41.0
37	36.779209454075264	39.0	35.0	41.0	30.0	41.0
38	36.704602747415215	39.0	35.0	40.0	30.0	41.0
39	36.67524920689811	39.0	35.0	40.0	30.0	41.0
40	36.53942956804138	39.0	35.0	40.0	30.0	41.0
41	36.616587231184816	39.0	35.0	40.0	30.0	41.0
42	36.48762610695975	39.0	35.0	40.0	30.0	41.0
43	36.45862839730771	39.0	35.0	40.0	30.0	41.0
44	36.37548425573715	39.0	35.0	40.0	30.0	41.0
45	36.196313391769785	39.0	35.0	40.0	30.0	41.0
46	36.02756937448943	38.0	35.0	40.0	29.0	41.0
47	35.93339698195286	38.0	35.0	40.0	29.0	41.0
48	35.91420466789955	38.0	35.0	40.0	29.0	41.0
49	35.816078042228604	38.0	35.0	40.0	28.0	41.0
50	35.77105290854144	38.0	35.0	40.0	28.0	41.0
51	34.46113675432134	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	0.0
13	6.0
14	5.0
15	12.0
16	33.0
17	80.0
18	171.0
19	326.0
20	546.0
21	954.0
22	1262.0
23	1644.0
24	1979.0
25	2371.0
26	2669.0
27	3227.0
28	4072.0
29	5170.0
30	6988.0
31	9230.0
32	12285.0
33	16117.0
34	22898.0
35	30563.0
36	37499.0
37	54422.0
38	85328.0
39	99181.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.42251657620544	36.16319105110433	19.669929284880194	25.744363087810036
2	19.920514401126628	35.51016623816612	19.831807269975393	24.737512090731858
3	19.62256869790962	31.361980223822627	24.322292553111517	24.69315852515624
4	21.8522750622704	30.742032646228946	19.14796048773887	28.257731803761782
5	16.52558724621992	36.655089634296075	18.998862343572238	27.820460775911755
6	20.121483664356273	35.20069361960177	21.99285331248465	22.684969403557307
7	79.71338074403734	8.58629900818411	6.779079149814818	4.921241097963745
8	81.93381545909699	4.674915928693499	4.514792039412027	8.87647657279748
9	76.01875379009988	9.511208672249703	9.375391539244134	5.094645998406279
10	28.861391348799447	43.44193692271453	12.964271574125583	14.73240015436043
11	18.32178135947437	26.978745370439977	36.07197806879063	18.627495201295023
12	22.665423764490082	23.218966286278462	36.14565009296708	17.969959856264378
13	23.261315170924107	26.686563124896633	28.79298161206417	21.25914009211509
14	17.565014308410138	28.145219086567135	33.62100504678424	20.668761558238486
15	16.20809590393569	31.259240326161585	29.373336741290913	23.15932702861181
16	19.29831155748673	29.939909689124107	29.773270586820228	20.988508166568938
17	17.01297529731924	30.043150757017635	30.744037327158917	22.199836618504207
18	18.174938481353962	28.568206762791114	28.564949156279912	24.691905599575005
19	18.749780738023283	31.29607633824981	31.07255441455799	18.88158850916891
20	20.874491938676808	31.27502718848511	28.17854690702791	19.67193396581017
21	19.47046353234803	29.608385580330072	29.103456571093506	21.81769431622839
22	19.206847990056783	29.621165421258638	29.22950088456546	21.942485704119118
23	19.515318268156147	29.024522259475876	27.08900282158841	24.37115665077957
24	16.277257396019706	32.49437436414026	28.604541604646847	22.623826635193176
25	18.959019310089058	30.69467205925837	26.671026847689355	23.67528178296322
26	22.42611497847474	31.76316699493317	24.905153533500723	20.90556449309137
27	17.269825041471837	29.593350473355283	28.8541243804283	24.282700104744578
28	20.192148667137765	29.87225170773757	28.06027073215959	21.875328892965072
29	20.976730666105357	29.18790375526855	28.177544566562922	21.657821012063167
30	20.78302837124686	27.926207694967747	27.46638400665554	23.82437992712985
31	21.506968772082814	30.051670650970014	23.834403331779704	24.606957245167465
32	20.20668260388006	26.861471536036646	29.305678759904374	23.626167100178918
33	19.907483975081817	28.34543659444804	25.46871945993896	26.27835997053119
34	19.258217938887302	26.780031373256552	27.364395864343244	26.5973548235129
35	19.06652032495878	27.212290698781654	31.1038775540888	22.61731142217077
36	20.177364145279228	26.741942435587095	29.27836498223352	23.80232843690016
37	18.97856494915628	25.729327980835247	30.491948700215005	24.80015836979347
38	19.831807269975393	25.594012018062173	30.23960948815484	24.33457122380759
39	19.368224804919485	24.360130905664725	30.555847904857842	25.715796384557944
40	20.583061448482205	26.78805009697644	27.734008910806736	24.89487954373462
41	18.867806327775355	23.978740358737653	32.048834027454106	25.104619286032886
42	22.340665453834703	26.110718527762327	28.479750216756127	23.068865801646847
43	20.37457463176517	25.047987049761193	29.928132188660523	24.649306129813116
44	20.736419539625025	24.413505535425216	28.149228448427078	26.70084647652268
45	23.438729433226584	24.80216305072344	27.870828384277285	23.888279131772688
46	18.458851418061172	24.34158760706249	33.43181328401818	23.767747690858155
47	19.913247432755483	25.870156816165746	28.107631319130167	26.1089644319486
48	19.49652438443766	23.070369312344326	33.563871640280055	23.86923466293796
49	20.616138683826733	23.160830539309288	31.48476693078338	24.7382638460806
50	19.883678389038405	22.112632998050447	29.077896889236364	28.92579172367478
51	19.111375060766893	21.73850941949452	33.18022582730676	25.96988969243183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2037.0
1	1607.5
2	1178.0
3	810.0
4	442.0
5	438.5
6	435.0
7	452.0
8	469.0
9	538.0
10	607.0
11	678.0
12	749.0
13	821.0
14	893.0
15	960.0
16	1027.0
17	1126.0
18	1225.0
19	1177.5
20	1130.0
21	1303.5
22	1477.0
23	1692.0
24	1907.0
25	2451.5
26	3804.0
27	4612.0
28	5394.0
29	6176.0
30	6917.0
31	7658.0
32	9788.5
33	11919.0
34	12493.0
35	13067.0
36	13828.5
37	14590.0
38	15946.5
39	17303.0
40	18251.0
41	19199.0
42	21882.5
43	24566.0
44	33067.5
45	41569.0
46	41649.5
47	41730.0
48	42955.0
49	44180.0
50	40305.5
51	36431.0
52	31873.5
53	27316.0
54	24444.5
55	21573.0
56	18119.5
57	14666.0
58	12802.0
59	10938.0
60	9600.5
61	8263.0
62	7311.0
63	6359.0
64	5322.5
65	4286.0
66	3310.5
67	2335.0
68	1785.0
69	1235.0
70	1103.0
71	971.0
72	883.0
73	795.0
74	564.0
75	240.0
76	147.0
77	124.5
78	102.0
79	75.5
80	49.0
81	74.0
82	99.0
83	55.0
84	11.0
85	10.0
86	9.0
87	6.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.594671976748515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23326638958649	24.847983116846592
2	10.58921123972653	6.903037337027694
3	3.7202080047051576	3.6377687879591614
4	1.806923638553878	2.3558433314278617
5	1.0489175942779496	1.7094562458064975
6	0.6805343369114468	1.33090760883256
7	0.4886906035701492	1.1150096945041783
8	0.4157324801216604	1.0840531055716514
9	0.2947272401671326	0.864588394427406
>10	3.91207330426863	30.00416882449617
>50	0.7135421021667576	15.487586321155684
>100	0.08609040580490228	4.465725587020357
>500	0.0054291246903992426	1.4038026710854254
>1k	0.004653535448913637	4.790068973838761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGT	4464	1.1186119589240877	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC	4246	1.063984403582365	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	3810	0.9547292928989191	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	3621	0.9073687059283427	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1730	0.43351225110633324	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT	1077	0.26988017019741095	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	931	0.23329474322543137	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	814	0.20397628462459844	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTTC	806	0.20197160369462694	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTAT	789	0.19771165671843755	No Hit
AACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	788	0.19746107160219115	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT	713	0.17866718788370844	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	712	0.17841660276746202	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	463	0.1160209088220996	No Hit
TACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	431	0.10800218510221367	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.10649867440473507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0176261570767742	0.0
2	0.0	0.0	0.0	1.4255787263259712	0.0
3	0.0	0.0	0.0	2.0405146015947238	0.0
4	0.0	0.0	0.0	4.953566577959536	0.0
5	0.0	0.0	0.0	5.3184185072143455	0.0
6	0.0	0.0	0.0	7.132905333954785	0.0
7	0.0	0.0	0.0	8.428680970065102	0.0
8	0.0	0.0	0.0	9.278916269489258	0.0
9	0.0	0.0	0.0	11.598833275698757	0.0
10	0.0	0.0	0.0	13.443390316388768	0.0
11	0.0	0.0	0.0	16.288533726250794	0.0
12	0.0	0.0	0.0	17.306911638676308	0.0
13	0.0	0.0	0.0	17.766234156756024	0.0
14	0.0	0.0	0.0	18.374905404118618	0.0
15	0.0	0.0	0.0	18.77483924964793	0.0
16	0.0	0.0	0.0	19.491512682112734	0.0
17	0.0	0.0	0.0	20.457518305242743	0.0
18	0.0	0.0	0.0	21.93246229946926	0.0
19	0.0	0.0	0.0	22.50329519427864	0.0
20	0.0	0.0	0.0	23.086406759784097	0.0
21	0.0	0.0	0.0	23.72715290202623	0.0
22	0.0	0.0	0.0	24.427788887051264	0.0
23	0.0	0.0	0.0	25.12115790370515	0.0
24	0.0	0.0	0.0	25.619822285035557	0.0
25	0.0	0.0	0.0	26.033287726842175	0.0
26	0.0	0.0	0.0	26.428209870046558	0.0
27	0.0	0.0	0.0	26.905825101612265	0.0
28	0.0	0.0	0.0	27.370911077365648	0.0
29	0.0	0.0	0.0	27.8487768940476	0.0
30	0.0	0.0	0.0	28.528614314424182	0.0
31	0.0	0.0	0.0	28.985430981341434	0.0
32	0.0	0.0	0.0	29.39112828454441	0.0
33	0.0	0.0	0.0	29.791312715189967	0.0
34	0.0	0.0	0.0	30.20578049746157	0.0
35	0.0	0.0	0.0	30.659590142983866	0.0
36	0.0	0.0	0.0	31.083580159672834	0.0
37	0.0	0.0	0.0	31.510827782873008	0.0
38	0.0	0.0	0.0	31.929806097237048	0.0
39	0.0	0.0	0.0	32.36958297624954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATA	40	6.7957444E-9	45.0	13
CGGGTAT	45	3.8380676E-10	45.0	6
AATCACG	20	7.027746E-4	45.0	1
CGAGTCA	20	7.027746E-4	45.0	1
GCGGTCG	20	7.027746E-4	45.0	12
CGTGATA	20	7.027746E-4	45.0	2
ATTACGG	35	1.2092642E-7	45.0	2
CACGCGC	20	7.027746E-4	45.0	2
GATTTCG	35	1.2092642E-7	45.0	5
TTGTAGG	45	3.8380676E-10	45.0	2
TAAGTCG	20	7.027746E-4	45.0	1
TTCCGCG	20	7.027746E-4	45.0	1
TACGGAT	35	1.2092642E-7	45.0	4
TTATCCG	20	7.027746E-4	45.0	1
TCTAGCG	45	3.8380676E-10	45.0	1
TCGGCAA	20	7.027746E-4	45.0	24
ATCGTGC	20	7.027746E-4	45.0	11
CTGCGTT	20	7.027746E-4	45.0	39
CATTAGT	25	3.8862134E-5	45.0	26
TAGTTCG	35	1.2092642E-7	45.0	1
>>END_MODULE