##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	996495
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8967390704419	33.0	31.0	34.0	30.0	34.0
2	32.1048103603129	34.0	31.0	34.0	30.0	34.0
3	32.26065258731855	34.0	31.0	34.0	30.0	34.0
4	35.897371286358684	37.0	35.0	37.0	35.0	37.0
5	35.65776546796522	37.0	35.0	37.0	33.0	37.0
6	34.96834203884615	37.0	35.0	37.0	33.0	37.0
7	35.36941279183538	37.0	35.0	37.0	33.0	37.0
8	35.784759582336086	37.0	35.0	37.0	35.0	37.0
9	37.497680369695786	39.0	37.0	39.0	35.0	39.0
10	37.05495461592883	39.0	37.0	39.0	33.0	39.0
11	37.03094947792011	39.0	37.0	39.0	33.0	39.0
12	36.873485566912024	39.0	37.0	39.0	33.0	39.0
13	36.86806757685688	39.0	37.0	39.0	33.0	39.0
14	38.06619401000507	40.0	37.0	41.0	33.0	41.0
15	38.10326193307543	40.0	37.0	41.0	33.0	41.0
16	37.99458200994486	40.0	37.0	41.0	33.0	41.0
17	37.99656194963347	40.0	37.0	41.0	33.0	41.0
18	37.979753034385524	40.0	37.0	41.0	33.0	41.0
19	38.01077376203594	40.0	37.0	41.0	33.0	41.0
20	37.945186880014454	40.0	37.0	41.0	33.0	41.0
21	37.94166754474433	40.0	37.0	41.0	33.0	41.0
22	37.92476028479822	40.0	37.0	41.0	33.0	41.0
23	37.824485822808946	40.0	37.0	41.0	33.0	41.0
24	37.65523861133272	40.0	36.0	41.0	33.0	41.0
25	37.712881650183895	40.0	36.0	41.0	33.0	41.0
26	37.6709115449651	40.0	36.0	41.0	33.0	41.0
27	37.557031395039616	40.0	36.0	41.0	33.0	41.0
28	37.532861680189065	40.0	36.0	41.0	33.0	41.0
29	37.344741318320715	39.0	36.0	41.0	32.0	41.0
30	37.409694980908085	39.0	36.0	41.0	32.0	41.0
31	37.351137737770884	39.0	36.0	41.0	32.0	41.0
32	37.308137020256	39.0	36.0	41.0	32.0	41.0
33	37.08702702973924	39.0	35.0	41.0	31.0	41.0
34	37.05860240141697	39.0	35.0	41.0	31.0	41.0
35	37.03076483073171	39.0	35.0	41.0	31.0	41.0
36	36.924881710394935	39.0	35.0	41.0	31.0	41.0
37	36.835160236629385	39.0	35.0	41.0	31.0	41.0
38	36.63313614217834	39.0	35.0	41.0	31.0	41.0
39	36.625521452691686	39.0	35.0	41.0	31.0	41.0
40	36.540413148084035	39.0	35.0	40.0	30.0	41.0
41	36.47176754524609	39.0	35.0	40.0	30.0	41.0
42	36.42433027762307	39.0	35.0	40.0	30.0	41.0
43	36.33843120136077	38.0	35.0	40.0	30.0	41.0
44	36.26013978996382	38.0	35.0	40.0	30.0	41.0
45	36.06685231737239	38.0	35.0	40.0	30.0	41.0
46	35.91810696491201	38.0	35.0	40.0	29.0	41.0
47	35.82290227246499	38.0	34.0	40.0	29.0	41.0
48	35.712915769773055	38.0	34.0	40.0	29.0	41.0
49	35.61089719466731	37.0	34.0	40.0	29.0	41.0
50	35.50956903948339	37.0	34.0	40.0	29.0	41.0
51	34.28024224908304	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	11.0
12	17.0
13	14.0
14	36.0
15	74.0
16	97.0
17	195.0
18	350.0
19	616.0
20	1078.0
21	1580.0
22	2275.0
23	2926.0
24	3709.0
25	4918.0
26	6101.0
27	7894.0
28	10184.0
29	13254.0
30	17380.0
31	22617.0
32	30091.0
33	41279.0
34	64374.0
35	78667.0
36	94244.0
37	136690.0
38	204706.0
39	251047.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.633420137582227	31.972965243177338	20.94942774424357	26.444186874996866
2	24.47147251115159	30.111942357964665	19.94761639546611	25.46896873541764
3	20.92213207291557	29.812492787219202	21.90848925483821	27.356885885027022
4	22.28651423238451	30.607980973311456	19.068535215931842	28.036969578372194
5	18.757946602842964	33.969964726365916	17.23009147060447	30.041997200186653
6	19.84064144827621	37.2072112755207	20.167888449013795	22.784258827189298
7	86.35989141942508	5.543730776371181	4.417382927159695	3.6789948770440395
8	87.53360528652928	3.9980130356900934	3.538602802823898	4.929778874956723
9	81.94571974771574	7.000536881770605	6.390599049669091	4.663144320844561
10	38.45659034917385	33.09660359560259	12.629968037973097	15.816838017250461
11	29.453434287176556	27.91393835393053	22.611453143267152	20.021174215625766
12	28.212083352149282	24.45069970245711	27.92196649255641	19.415250452837192
13	26.072684760084098	25.016783827314736	26.132996151511044	22.777535261090122
14	22.275676245239566	26.654122700063727	27.970135324311713	23.100065730385
15	21.42188370237683	28.274201074767056	26.828433659978224	23.47548156287789
16	24.170216609215302	26.67017897731549	27.16581618573099	21.99378822773822
17	23.381552340955047	26.58859301852995	26.697575000376318	23.332279640138687
18	24.289133412611204	26.373238199890615	25.74654162840757	23.59108675909061
19	24.772427357889402	28.188199639737277	25.249599847465365	21.78977315490795
20	27.335410614202782	26.86957787043588	24.32847129187803	21.46654022348331
21	25.88572948183383	26.41980140392074	25.09706521357358	22.597403900671857
22	25.32626857134256	25.403840460815157	24.65501583048585	24.614875137356435
23	24.389384793701925	27.526179258300342	24.15094907651318	23.93348687148455
24	23.11772763536194	27.726180261817674	25.9687203648789	23.187371737941483
25	24.73610003060728	26.861349028344346	24.03785267362104	24.364698267427332
26	26.858238124626816	26.258736872738954	23.27517950416209	23.607845498472145
27	23.778543796004996	26.35748297783732	25.27237969081631	24.59159353534137
28	23.69856346494463	28.144446284226216	25.210563023397007	22.94642722743215
29	23.222896251361018	28.965423810455647	23.255811619727144	24.555868318456188
30	25.492049633967056	25.968318957947606	24.540815558532657	23.99881584955268
31	25.930185299474655	27.991209188204657	22.065941123638353	24.01266438868233
32	25.034546084024505	27.32838599290513	23.96680364678197	23.670264276288393
33	25.351456856281267	27.126679009929806	23.51401662828213	24.007847505506803
34	23.363388677313985	28.193217226378458	24.711413504332686	23.73198059197487
35	25.656726827530495	26.40635427172239	24.294050647519555	23.64286825322756
36	24.249895885077198	30.742652998760654	23.36248551171857	21.644965604443573
37	24.32927410574062	30.735226970531716	22.313910255445336	22.62158866828233
38	25.05301080286404	29.28383985870476	23.559275259785547	22.103874078645653
39	24.41467343037346	26.899081279886	24.11050732818529	24.57573796155525
40	26.242981650685653	26.622210849025834	24.51934028770842	22.615467212580093
41	22.858318406013076	26.40755849251627	26.426424618287097	24.30769848318356
42	25.4010306122961	26.808764720344808	23.53017325726672	24.260031410092374
43	24.14021144110106	25.67047501492732	25.482716922814465	24.706596621157157
44	24.56329434668513	24.69816707559998	25.240768894976895	25.497769682737996
45	25.43133683560881	25.59320418065319	25.59270242198907	23.38275656174893
46	22.814565050502008	25.778654182911104	26.95517789853436	24.451602868052525
47	23.703480699852985	27.49085544834645	24.978248761910496	23.827415089890064
48	23.211255450353487	26.241777429891773	26.933702627710126	23.613264492044618
49	24.378346103091335	26.338917907264964	25.76892006482722	23.51381592481648
50	23.29956497523821	25.469470494081758	25.796717494819344	25.43424703586069
51	23.27156684178044	24.692447026829036	27.034656470930614	25.001329660459916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1177.0
1	969.5
2	762.0
3	560.0
4	358.0
5	382.0
6	406.0
7	399.0
8	392.0
9	439.5
10	487.0
11	588.0
12	689.0
13	719.0
14	749.0
15	849.0
16	949.0
17	964.0
18	979.0
19	1289.5
20	1600.0
21	1981.0
22	2362.0
23	3378.5
24	4395.0
25	5156.0
26	6569.0
27	7221.0
28	9651.5
29	12082.0
30	13706.0
31	15330.0
32	17869.5
33	20409.0
34	22586.0
35	24763.0
36	27509.0
37	30255.0
38	32456.0
39	34657.0
40	36024.5
41	37392.0
42	40556.5
43	43721.0
44	54061.0
45	64401.0
46	68097.0
47	71793.0
48	75174.5
49	78556.0
50	78946.0
51	79336.0
52	76352.0
53	73368.0
54	68880.0
55	64392.0
56	61495.0
57	58598.0
58	56589.0
59	54580.0
60	53307.5
61	52035.0
62	47539.0
63	43043.0
64	37103.0
65	31163.0
66	27634.0
67	24105.0
68	20588.0
69	17071.0
70	14436.5
71	11802.0
72	10376.0
73	8950.0
74	8093.5
75	5567.0
76	3897.0
77	3276.5
78	2656.0
79	1972.5
80	1289.0
81	940.5
82	592.0
83	429.5
84	267.0
85	192.5
86	118.0
87	99.5
88	81.0
89	66.0
90	51.0
91	35.0
92	19.0
93	13.5
94	8.0
95	12.0
96	16.0
97	14.0
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	996495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.276409219853647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75557442252521	16.8756217652125
2	9.327954296432209	4.155866541828318
3	3.2700069710817314	2.1853204031877236
4	1.5308574663028525	1.3640802950652264
5	0.9161880015331504	1.0204689422236182
6	0.6383800088413896	0.8532488588834751
7	0.49496608054146696	0.7718246872062131
8	0.3675037685864307	0.6549331471095782
9	0.3255394464325158	0.6526664933341814
>10	5.034397383906098	28.819468033121275
>50	2.03996569034568	31.732351154978478
>100	0.289909267783085	8.421144331397276
>500	0.006452670507095692	0.9548597554468435
>1k	0.0023045251811056044	1.5381455910052864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	3663	0.3675883973326509	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGT	3532	0.35444232033276635	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	3324	0.33356915990546865	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	2922	0.29322776331040296	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1540	0.15454166854826165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0070346564709306E-4	0.0	0.0	0.34219940892829365	0.0
2	2.0070346564709306E-4	0.0	0.0	0.47476404798819866	0.0
3	2.0070346564709306E-4	0.0	0.0	0.7058740886808264	0.0
4	2.0070346564709306E-4	0.0	0.0	1.685407352771464	0.0
5	2.0070346564709306E-4	0.0	0.0	1.8169684745031336	0.0
6	2.0070346564709306E-4	0.0	0.0	2.522340804519842	0.0
7	2.0070346564709306E-4	0.0	0.0	3.0484849397136964	0.0
8	2.0070346564709306E-4	0.0	0.0	3.5086979864424808	0.0
9	2.0070346564709306E-4	0.0	0.0	4.40734775387734	0.0
10	2.0070346564709306E-4	0.0	0.0	5.248696681869954	0.0
11	2.0070346564709306E-4	0.0	0.0	6.3568808674403785	0.0
12	2.0070346564709306E-4	0.0	0.0	6.732497403398913	0.0
13	2.0070346564709306E-4	0.0	0.0	6.909216804901178	0.0
14	2.0070346564709306E-4	0.0	0.0	7.134004686425923	0.0
15	2.0070346564709306E-4	0.0	0.0	7.306107908218305	0.0
16	2.0070346564709306E-4	0.0	0.0	7.616395466108711	0.0
17	2.0070346564709306E-4	0.0	0.0	7.991108836471834	0.0
18	2.0070346564709306E-4	0.0	0.0	8.510930812497804	0.0
19	2.0070346564709306E-4	0.0	0.0	8.73662185961796	1.0035173282354653E-4
20	2.0070346564709306E-4	0.0	0.0	8.983587474096709	1.0035173282354653E-4
21	2.0070346564709306E-4	0.0	0.0	9.271998354231581	1.0035173282354653E-4
22	2.0070346564709306E-4	0.0	0.0	9.577770084144928	1.0035173282354653E-4
23	2.0070346564709306E-4	0.0	0.0	9.905619195279455	1.0035173282354653E-4
24	2.0070346564709306E-4	0.0	0.0	10.143252098605613	1.0035173282354653E-4
25	2.0070346564709306E-4	0.0	0.0	10.353488978870942	1.0035173282354653E-4
26	2.0070346564709306E-4	0.0	0.0	10.56111671408286	1.0035173282354653E-4
27	2.0070346564709306E-4	0.0	0.0	10.770550780485602	1.0035173282354653E-4
28	2.0070346564709306E-4	0.0	0.0	10.995539365475993	1.0035173282354653E-4
29	2.0070346564709306E-4	0.0	0.0	11.245515531939448	1.0035173282354653E-4
30	2.0070346564709306E-4	0.0	0.0	11.57888398837927	1.0035173282354653E-4
31	2.0070346564709306E-4	0.0	0.0	11.800661317919307	1.0035173282354653E-4
32	2.0070346564709306E-4	0.0	0.0	12.04491743561182	1.0035173282354653E-4
33	2.0070346564709306E-4	0.0	0.0	12.281446469876919	1.0035173282354653E-4
34	2.0070346564709306E-4	0.0	0.0	12.499410433569661	1.0035173282354653E-4
35	2.0070346564709306E-4	0.0	0.0	12.752497503750647	1.0035173282354653E-4
36	2.0070346564709306E-4	0.0	0.0	12.980998399389861	1.0035173282354653E-4
37	2.0070346564709306E-4	0.0	0.0	13.215620750731313	1.0035173282354653E-4
38	2.0070346564709306E-4	0.0	0.0	13.456665612973472	1.0035173282354653E-4
39	2.0070346564709306E-4	0.0	0.0	13.72992338145199	1.0035173282354653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	35	1.2117016E-7	45.0	1
ATTACGA	20	7.032834E-4	45.0	31
ATAGCCG	45	3.8380676E-10	45.0	1
CGACACT	20	7.032834E-4	45.0	38
TCTAGCG	45	3.8380676E-10	45.0	1
CGTTACG	50	2.1827873E-11	45.0	1
ACGATAG	20	7.032834E-4	45.0	1
TCGGACG	35	1.2117016E-7	45.0	1
TACGATT	25	3.890431E-5	45.0	11
GATATCG	20	7.032834E-4	45.0	38
TATTACG	25	3.890431E-5	45.0	1
GTCCGAC	20	7.032834E-4	45.0	21
CGCACTA	25	3.890431E-5	45.0	13
TCATACG	20	7.032834E-4	45.0	1
CAGTACG	25	3.890431E-5	45.0	1
CGATCAC	20	7.032834E-4	45.0	30
TACGTTA	20	7.032834E-4	45.0	35
AATCGTC	20	7.032834E-4	45.0	13
CTCGTTA	30	2.1652104E-6	44.999996	45
CATACCG	60	0.0	44.999996	1
>>END_MODULE