FastQCFastQC Report
Sat 18 Jun 2016
SRR3553234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250762
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT22550.8992590583900272No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC15200.6061524473405061No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13870.5531141081982118No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT13090.5220089168215281No Hit
AATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT12270.48930858742552696No Hit
AAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT6460.2576147901197151No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT5020.20018982142429873No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC4210.16788827653312705No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.14156849921439452No Hit
AGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC3470.13837822317576026No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT3230.12880739505985755No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3050.12162927397293051No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT2840.11325479937151561No Hit
AGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT2770.11046330783771065No Hit
AACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG2710.10807060080873497No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT2660.10607667828458857No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT2600.1036839712556129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA302.158693E-645.00000430
GTCGAGG302.158693E-645.0000042
TATGGGA950.045.0000044
TATGCGG302.158693E-645.0000042
ATGACGG302.158693E-645.0000042
CATAAGG600.045.0000042
CATAAGA302.158693E-645.00000420
GCAATTC302.158693E-645.00000424
ACGTAAG302.158693E-645.0000041
ATAGTGG302.158693E-645.0000042
ATACGAG302.158693E-645.0000041
CAATCGG302.158693E-645.0000042
CGATAGG302.158693E-645.0000042
TTAGGCC207.0227275E-445.028
TAATAGG207.0227275E-445.02
TCGTTGC207.0227275E-445.016
CTCACAT253.882056E-545.032
CCCTCGG207.0227275E-445.02
GTACAGG253.882056E-545.02
GATCGCC207.0227275E-445.021
Produced by FastQC (version 0.11.5)