##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250762
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70133034510811	31.0	31.0	34.0	30.0	34.0
2	31.9055159872708	33.0	31.0	34.0	30.0	34.0
3	32.07392268366021	34.0	31.0	34.0	30.0	34.0
4	35.74906086249113	37.0	35.0	37.0	35.0	37.0
5	35.49391853630135	37.0	35.0	37.0	33.0	37.0
6	34.84069755385585	37.0	35.0	37.0	32.0	37.0
7	35.18203715076447	37.0	35.0	37.0	33.0	37.0
8	35.59442020720843	37.0	35.0	37.0	33.0	37.0
9	37.22341104314051	39.0	37.0	39.0	34.0	39.0
10	36.88256593901787	39.0	37.0	39.0	33.0	39.0
11	36.93571593782152	39.0	37.0	39.0	33.0	39.0
12	36.82314305995326	39.0	37.0	39.0	33.0	39.0
13	36.76070537003214	39.0	35.0	39.0	33.0	39.0
14	37.902182148810425	40.0	37.0	41.0	33.0	41.0
15	37.95497324155973	40.0	37.0	41.0	33.0	41.0
16	37.81286239541876	40.0	37.0	41.0	33.0	41.0
17	37.811008047471304	40.0	37.0	41.0	33.0	41.0
18	37.733639865689376	39.0	36.0	41.0	33.0	41.0
19	37.71598567566058	39.0	36.0	41.0	33.0	41.0
20	37.654078369130886	39.0	36.0	41.0	33.0	41.0
21	37.63457381899969	39.0	36.0	41.0	33.0	41.0
22	37.585894194495175	39.0	36.0	41.0	33.0	41.0
23	37.475666169515314	39.0	36.0	41.0	32.0	41.0
24	37.312343975562484	39.0	36.0	41.0	32.0	41.0
25	37.313955064961995	39.0	36.0	41.0	32.0	41.0
26	37.29462996785797	39.0	36.0	41.0	32.0	41.0
27	37.216013590575926	39.0	35.0	41.0	32.0	41.0
28	37.096944513124	39.0	35.0	41.0	31.0	41.0
29	36.823318525135385	39.0	35.0	40.0	31.0	41.0
30	36.932338233065614	39.0	35.0	40.0	31.0	41.0
31	36.812926200939536	39.0	35.0	40.0	31.0	41.0
32	36.70009411314314	39.0	35.0	40.0	31.0	41.0
33	36.53707898325903	39.0	35.0	40.0	30.0	41.0
34	36.48378940987869	39.0	35.0	40.0	30.0	41.0
35	36.429299495138814	39.0	35.0	40.0	30.0	41.0
36	36.34501639004315	39.0	35.0	40.0	30.0	41.0
37	36.165439739673474	38.0	35.0	40.0	30.0	41.0
38	35.941095540791665	38.0	35.0	40.0	29.0	41.0
39	35.8366060248363	38.0	35.0	40.0	29.0	41.0
40	35.76224467822078	38.0	35.0	40.0	29.0	41.0
41	35.700903645687944	38.0	35.0	40.0	28.0	41.0
42	35.659605522367826	38.0	35.0	40.0	28.0	41.0
43	35.62865585694802	38.0	35.0	40.0	28.0	41.0
44	35.53634123192509	38.0	35.0	40.0	28.0	41.0
45	35.27701166843461	38.0	34.0	40.0	27.0	41.0
46	35.035420039718936	37.0	34.0	40.0	26.0	41.0
47	34.951886649492344	37.0	34.0	40.0	26.0	41.0
48	34.78994424992622	37.0	34.0	40.0	26.0	41.0
49	34.69834743701199	37.0	33.0	40.0	26.0	41.0
50	34.63610913934328	37.0	34.0	40.0	26.0	41.0
51	33.20912259433248	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	5.0
12	5.0
13	3.0
14	15.0
15	19.0
16	32.0
17	74.0
18	131.0
19	276.0
20	461.0
21	702.0
22	1001.0
23	1260.0
24	1623.0
25	1796.0
26	2026.0
27	2516.0
28	3061.0
29	3891.0
30	5029.0
31	6458.0
32	8440.0
33	11542.0
34	16928.0
35	21998.0
36	25064.0
37	35659.0
38	49899.0
39	50823.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.065887175887895	35.34626458554327	19.77093818042606	25.816910058142785
2	23.22919740630558	32.81198905735319	19.606240179931568	24.352573356409664
3	20.427337475375058	30.706805656359414	21.963854172482275	26.90200269578325
4	21.659182810792704	31.32851069938826	19.121717006563994	27.89058948325504
5	17.684098866654438	37.10530303634522	17.59277721504853	27.61782088195181
6	19.64532106140484	37.229723801851954	19.945605793541286	23.17934934320192
7	78.95813560268302	8.963878099552563	6.775348737049473	5.302637560714941
8	80.62984024692736	6.307973297389556	5.383989599700114	7.678196855982963
9	73.87722222665316	9.684082915274244	10.184557468834992	6.254137389237603
10	35.018862507078424	32.42078145811567	13.534347309400946	19.026008725404967
11	24.94835740662461	29.111667637042295	25.597977364991504	20.341997591341592
12	25.13100070983642	22.882254887104107	31.542259193976758	20.444485209082718
13	24.52564583150557	25.454813727757795	27.234190188306044	22.785350252430593
14	20.003429546741533	28.33324028361554	29.437873361992644	22.225456807650282
15	18.321755289876457	29.16430719167976	28.674599819749403	23.83933769869438
16	20.39304200795974	27.791690926057377	28.57091584849379	23.244351217489093
17	20.956125728778684	27.66846651406513	25.97881656710347	25.39659119005272
18	21.939927102192517	26.196951691245086	26.34210925100294	25.52101195555945
19	22.189964986720476	29.421921981799475	26.576594539842556	21.81151849163749
20	21.742528772302023	28.29495697115193	25.532975490704334	24.429538765841716
21	23.0537322241807	28.390266467806125	26.49245100932358	22.063550298689595
22	20.78624352972141	27.042773625987987	25.664574377297996	26.50640846699261
23	22.083489523931057	28.115503943978755	22.816854228312106	26.984152303778085
24	20.241902680629444	29.557109928936605	25.278152192118426	24.922835198315536
25	22.648168382769317	27.471865753184293	24.874183488726363	25.005782375320024
26	23.217632655665533	27.453122881457322	23.467271755688664	25.861972707188492
27	21.20137819924869	25.315637935572376	25.22511385297613	28.257870012202808
28	23.5685630199153	25.15173750408754	28.03056284445012	23.249136631547042
29	22.628627942032683	29.49769103771704	22.263341335609063	25.610339684641215
30	24.006428406217847	26.67030889847744	22.966797202127914	26.356465493176795
31	24.02118343289653	28.880771408746142	21.774032748183537	25.32401241017379
32	23.021829463794354	28.032157982469435	22.672893022068735	26.273119531667476
33	25.166093746261396	24.855440616999385	23.65190898142462	26.326556655314604
34	20.288560467694467	27.364991505890046	24.816758520030945	27.529689506384543
35	23.44573739242788	25.282140037166716	25.31404279755306	25.958079772852344
36	26.006332697936692	26.013510819023615	24.35376970992415	23.62638677311554
37	23.870442890071065	29.563490481013872	20.528229955096865	26.037836673818205
38	25.355915170560134	28.529841044496372	24.097750057823752	22.01649372711974
39	24.21100485719527	27.71751700815913	24.325456010081272	23.746022124564327
40	24.48616616552747	27.959977987095336	23.624791635096226	23.929064212280966
41	21.02511544811415	26.520365924661633	27.036791858415548	25.417726768808674
42	23.220025362694507	26.563434651183194	24.746173662676163	25.470366323446136
43	21.37165918281079	22.679273574146002	27.171581021047846	28.777486221995357
44	24.054282546797364	22.42125999952146	24.72424051491055	28.800216938770628
45	27.114953621362087	23.753997814660913	26.03464639777957	23.09640216619743
46	22.40451105031863	24.70350372065943	29.023935045979854	23.86805018304209
47	23.66746157711296	24.52046163294279	25.366682352190523	26.445394437753727
48	21.726577392108855	24.611783284548697	29.20936984072547	24.452269482616984
49	22.562828498735854	24.40002871248435	27.15961748590297	25.87752530287683
50	22.693231031815028	22.301624648072675	25.686507525063607	29.31863679504869
51	21.243649356760592	22.545281980523367	30.198754197206913	26.012314465509128
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1629.0
1	1277.5
2	926.0
3	633.5
4	341.0
5	332.0
6	323.0
7	332.5
8	342.0
9	366.0
10	390.0
11	438.5
12	487.0
13	521.0
14	555.0
15	578.0
16	601.0
17	626.5
18	652.0
19	667.0
20	682.0
21	759.5
22	837.0
23	908.5
24	980.0
25	1219.0
26	1508.0
27	1558.0
28	2088.0
29	2618.0
30	2780.0
31	2942.0
32	3094.5
33	3247.0
34	4035.5
35	4824.0
36	5297.5
37	5771.0
38	5966.5
39	6162.0
40	6897.0
41	7632.0
42	9202.5
43	10773.0
44	14304.5
45	17836.0
46	20215.5
47	22595.0
48	24975.0
49	27355.0
50	25553.5
51	23752.0
52	20735.5
53	17719.0
54	16042.5
55	14366.0
56	13477.0
57	12588.0
58	12592.0
59	12596.0
60	12225.5
61	11855.0
62	10377.0
63	8899.0
64	7994.0
65	7089.0
66	6352.0
67	5615.0
68	4827.0
69	4039.0
70	3463.5
71	2888.0
72	2555.0
73	2222.0
74	1876.5
75	1263.0
76	995.0
77	784.5
78	574.0
79	450.0
80	326.0
81	227.0
82	128.0
83	78.5
84	29.0
85	22.0
86	15.0
87	13.5
88	12.0
89	6.0
90	0.0
91	3.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	250762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.518332123687003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5097930057189	24.744977309161676
2	9.111038007996356	5.743294438551295
3	3.223847360696391	3.048308754915019
4	1.5271521838149704	1.9253315893157656
5	0.8388582418138569	1.3219706335090644
6	0.6111139227693709	1.1556774949952544
7	0.41373551293081634	0.9128177315542227
8	0.3061895844931424	0.7720468013494868
9	0.3061895844931424	0.8685526515181726
>10	4.115845943620629	32.4307510707364
>50	0.9539956475530138	19.54482736618786
>100	0.07338428058100106	4.003796428485975
>500	0.0025304924338276228	0.4578046115440138
>1k	0.0063262310845690565	3.0698431181758004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	2255	0.8992590583900272	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	1520	0.6061524473405061	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1387	0.5531141081982118	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	1309	0.5220089168215281	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1227	0.48930858742552696	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	646	0.2576147901197151	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	502	0.20018982142429873	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	421	0.16788827653312705	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	355	0.14156849921439452	No Hit
AGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	347	0.13837822317576026	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	323	0.12880739505985755	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	305	0.12162927397293051	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	284	0.11325479937151561	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	277	0.11046330783771065	No Hit
AACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	271	0.10807060080873497	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	266	0.10607667828458857	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	260	0.1036839712556129	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0248761774112505	0.0
2	0.0	0.0	0.0	1.4136910696197988	0.0
3	0.0	0.0	0.0	1.913368054170887	0.0
4	0.0	0.0	0.0	4.184844593678468	0.0
5	0.0	0.0	0.0	4.511449103133649	0.0
6	0.0	0.0	0.0	6.110574967499063	0.0
7	0.0	0.0	0.0	7.341223949402222	0.0
8	0.0	0.0	0.0	8.409966422344693	0.0
9	0.0	0.0	0.0	10.113972611480209	0.0
10	0.0	0.0	0.0	12.130625852401879	0.0
11	0.0	0.0	0.0	14.213876105630039	0.0
12	0.0	0.0	0.0	15.04494301369426	0.0
13	0.0	0.0	0.0	15.43854331996076	0.0
14	0.0	0.0	0.0	15.93144096792975	0.0
15	0.0	0.0	0.0	16.26283089144288	0.0
16	0.0	0.0	0.0	16.95193051578788	0.0
17	0.0	0.0	0.0	17.75348737049473	0.0
18	0.0	0.0	0.0	19.18472495832702	0.0
19	0.0	0.0	0.0	19.607037748941227	0.0
20	0.0	0.0	0.0	20.203220583661	0.0
21	0.0	0.0	0.0	20.727622207511505	0.0
22	0.0	0.0	0.0	21.202973337268006	0.0
23	0.0	0.0	0.0	21.682312312072803	0.0
24	0.0	0.0	0.0	22.02566577073081	0.0
25	0.0	0.0	0.0	22.336318899992822	0.0
26	0.0	0.0	0.0	22.615866837878148	0.0
27	0.0	0.0	0.0	23.027412446861966	0.0
28	0.0	0.0	0.0	23.412638278527048	0.0
29	0.0	0.0	0.0	23.752003892136766	0.0
30	0.0	0.0	0.0	24.258460213269952	0.0
31	0.0	0.0	0.0	24.566720635502985	0.0
32	0.0	0.0	0.0	24.886944592880898	0.0
33	0.0	0.0	0.0	25.22591142198579	0.0
34	0.0	0.0	0.0	25.504263006356624	0.0
35	0.0	0.0	0.0	25.82368939472488	0.0
36	0.0	0.0	0.0	26.109219100182642	0.0
37	0.0	0.0	0.0	26.401129357717675	0.0
38	0.0	0.0	0.0	26.679480942088514	0.0
39	0.0	0.0	0.0	27.00967451208716	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	30	2.158693E-6	45.000004	30
GTCGAGG	30	2.158693E-6	45.000004	2
TATGGGA	95	0.0	45.000004	4
TATGCGG	30	2.158693E-6	45.000004	2
ATGACGG	30	2.158693E-6	45.000004	2
CATAAGG	60	0.0	45.000004	2
CATAAGA	30	2.158693E-6	45.000004	20
GCAATTC	30	2.158693E-6	45.000004	24
ACGTAAG	30	2.158693E-6	45.000004	1
ATAGTGG	30	2.158693E-6	45.000004	2
ATACGAG	30	2.158693E-6	45.000004	1
CAATCGG	30	2.158693E-6	45.000004	2
CGATAGG	30	2.158693E-6	45.000004	2
TTAGGCC	20	7.0227275E-4	45.0	28
TAATAGG	20	7.0227275E-4	45.0	2
TCGTTGC	20	7.0227275E-4	45.0	16
CTCACAT	25	3.882056E-5	45.0	32
CCCTCGG	20	7.0227275E-4	45.0	2
GTACAGG	25	3.882056E-5	45.0	2
GATCGCC	20	7.0227275E-4	45.0	21
>>END_MODULE