##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553233_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256046
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7047717988174	31.0	31.0	34.0	30.0	34.0
2	31.907715801066995	33.0	31.0	34.0	30.0	34.0
3	32.05588839505401	33.0	31.0	34.0	30.0	34.0
4	35.72577193160604	37.0	35.0	37.0	33.0	37.0
5	35.49625067370707	37.0	35.0	37.0	33.0	37.0
6	35.52869406278559	37.0	35.0	37.0	33.0	37.0
7	35.51313435866992	37.0	35.0	37.0	33.0	37.0
8	35.58856611702585	37.0	35.0	37.0	35.0	37.0
9	37.23688321629707	39.0	37.0	39.0	34.0	39.0
10	36.90120134663303	39.0	37.0	39.0	33.0	39.0
11	36.94015919014552	39.0	37.0	39.0	33.0	39.0
12	36.85099942978996	39.0	37.0	39.0	33.0	39.0
13	36.79585308889809	39.0	37.0	39.0	33.0	39.0
14	37.913671762105245	40.0	37.0	41.0	33.0	41.0
15	37.97796099138436	40.0	37.0	41.0	33.0	41.0
16	37.84496926333549	40.0	37.0	41.0	33.0	41.0
17	37.8120298696328	40.0	37.0	41.0	33.0	41.0
18	37.69655843090695	39.0	36.0	41.0	33.0	41.0
19	37.688844973168884	39.0	36.0	41.0	33.0	41.0
20	37.63968974324926	39.0	36.0	41.0	33.0	41.0
21	37.609468611108944	39.0	36.0	41.0	33.0	41.0
22	37.539305437304236	39.0	36.0	41.0	32.0	41.0
23	37.38844973168884	39.0	36.0	41.0	32.0	41.0
24	37.265553845793335	39.0	36.0	41.0	32.0	41.0
25	37.2667489435492	39.0	36.0	41.0	32.0	41.0
26	37.23137639330433	39.0	36.0	41.0	32.0	41.0
27	37.1749841825297	39.0	35.0	41.0	32.0	41.0
28	37.02540949673106	39.0	35.0	41.0	31.0	41.0
29	36.821688290385325	39.0	35.0	40.0	31.0	41.0
30	36.9178546042508	39.0	35.0	40.0	31.0	41.0
31	36.768385368254144	39.0	35.0	40.0	31.0	41.0
32	36.6279457597463	39.0	35.0	40.0	30.0	41.0
33	36.61546753317763	39.0	35.0	40.0	30.0	41.0
34	36.477148637354226	39.0	35.0	40.0	30.0	41.0
35	36.386754723760575	39.0	35.0	40.0	30.0	41.0
36	36.27094350233942	39.0	35.0	40.0	30.0	41.0
37	36.10179811440132	38.0	35.0	40.0	30.0	41.0
38	35.967794849363	38.0	35.0	40.0	29.0	41.0
39	35.80835474875608	38.0	35.0	40.0	29.0	41.0
40	35.668176811979095	38.0	35.0	40.0	28.0	41.0
41	35.567581606430096	38.0	35.0	40.0	28.0	41.0
42	35.52897526225756	38.0	34.0	40.0	28.0	41.0
43	35.47225498543231	38.0	34.0	40.0	28.0	41.0
44	35.376576865094556	38.0	34.0	40.0	27.0	41.0
45	35.13265194535357	38.0	34.0	40.0	26.0	41.0
46	34.89777227529428	37.0	34.0	40.0	26.0	41.0
47	34.78604625731314	37.0	34.0	40.0	26.0	41.0
48	34.65634300086703	37.0	33.0	40.0	25.0	41.0
49	34.49061887317123	37.0	33.0	40.0	24.0	41.0
50	34.412785202658895	37.0	33.0	40.0	24.0	41.0
51	32.913695195394574	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	2.0
13	6.0
14	9.0
15	18.0
16	40.0
17	82.0
18	140.0
19	289.0
20	482.0
21	706.0
22	1027.0
23	1331.0
24	1674.0
25	1977.0
26	2202.0
27	2716.0
28	3297.0
29	4113.0
30	5160.0
31	6746.0
32	8558.0
33	11752.0
34	17205.0
35	22303.0
36	25101.0
37	36237.0
38	50951.0
39	51906.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.970419377768057	35.663904142224446	19.54375385672887	25.82192262327863
2	23.038828960421174	33.26472586957031	19.462127898893165	24.234317271115348
3	20.471712114229472	30.72729118986432	22.184685564312662	26.61631113159354
4	21.64142380665974	31.505666950469834	18.925896128039494	27.927013114830928
5	17.423822281933717	37.430774157768525	17.57691977222843	27.56848378806933
6	20.20691594479117	36.093123891800694	20.22644368590019	23.473516477507946
7	78.42457995828875	9.156948360841412	6.987416323629347	5.4310553572404965
8	79.98953313076557	6.479695054794841	5.537676823695742	7.99309499074385
9	73.43446099528991	9.68654069971802	10.491864743053982	6.387133561938089
10	34.68478320301821	32.43128187903736	13.626457745873788	19.257477172070644
11	24.93926872515095	28.886996867750327	25.849652015653433	20.324082391445287
12	24.752974074970904	22.9208814041227	31.85052685845512	20.475617662451278
13	24.425298579161556	25.348570178796	27.425931277973486	22.800199964068955
14	20.04600735805285	28.175405981737654	29.781367410543417	21.997219249666074
15	18.19126250751818	29.399795349273177	28.477304859283098	23.931637283925543
16	20.222928692500567	27.850854924505754	28.620638479023302	23.30557790397038
17	20.852112511033173	27.89537817423432	26.10898041758122	25.143528897151292
18	21.885911125344666	26.341360536778545	25.99728173843762	25.77544659943916
19	22.00932644915367	29.575154464432174	26.751052545245773	21.664466541168384
20	21.737109738093935	28.32772236238801	25.5317403903986	24.403427509119453
21	22.68264296259266	28.576115229294736	26.466338079876273	22.274903728236335
22	20.633011255789977	27.14238847707053	25.86019699585231	26.36440327128719
23	22.28857314701265	27.98989244120197	22.793560532091888	26.927973879693496
24	19.858541043406262	29.713020316661847	25.203674339767073	25.224764300164814
25	22.19054388664537	27.40562242722011	25.205236559055795	25.198597127078727
26	23.360646133897813	27.482952282011823	23.431727111534645	25.724674472555716
27	21.03020550994743	25.30756192246706	25.503620443201612	28.158612124383897
28	23.662545011443257	25.13767057481859	27.96684970669333	23.232934707044826
29	22.365512446982184	29.59429165071901	22.196792763800254	25.843403138498548
30	23.919920639260134	26.654585504167223	22.86190762597346	26.56358623059919
31	24.133944681814988	28.64797731657593	21.78124243300032	25.436835568608767
32	22.86386040008436	28.137912718808337	22.580317599181395	26.417909281925905
33	25.11736172406521	24.845926122649836	23.84532466822368	26.19138748506128
34	20.18816931332651	27.685650234723447	24.40967638627434	27.7165040656757
35	23.460628168375994	25.623911328433174	24.996289729189286	25.91917077400155
36	25.950024604953796	26.042195542988367	24.53660670348297	23.471173148574863
37	23.788303664185342	29.86650836177874	20.269014161517852	26.07617381251806
38	25.502839333557255	28.562055255696244	24.011310467650343	21.923794943096162
39	24.37765089085555	27.96411582293807	24.133554126992806	23.52467915921358
40	24.541683916171316	28.034806245752712	23.48679534146208	23.936714496613888
41	20.984510595752326	26.38627434132929	27.172851753200593	25.456363309717783
42	23.414933254180887	26.556556243799946	24.695562516110385	25.33294798590878
43	21.42896198339361	22.68342407223702	27.191598384665255	28.696015559704115
44	24.021855447849212	22.319036423142716	24.764690719636313	28.894417409371755
45	27.262679362302087	23.69964771955039	25.787944353748937	23.249728564398584
46	22.506893292611487	24.494426782687487	29.30918663052733	23.6894932941737
47	23.652390586066566	24.552228896370185	25.1294689235528	26.66591159401045
48	21.714067003585296	24.4303757918499	29.456035243667156	24.39952196089765
49	22.599454785468236	24.133944681814988	27.168555650156613	26.098044882560167
50	22.793560532091888	22.1003257227217	25.74849831670872	29.357615428477697
51	21.214156831194394	22.190153331823186	30.2855736859783	26.310116151004117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1571.0
1	1314.0
2	1057.0
3	717.5
4	378.0
5	332.0
6	286.0
7	314.0
8	342.0
9	369.5
10	397.0
11	463.0
12	529.0
13	542.0
14	555.0
15	601.0
16	647.0
17	629.5
18	612.0
19	693.0
20	774.0
21	847.0
22	920.0
23	974.0
24	1028.0
25	1279.5
26	1537.0
27	1543.0
28	2089.5
29	2636.0
30	2837.5
31	3039.0
32	3154.0
33	3269.0
34	4012.0
35	4755.0
36	5209.5
37	5664.0
38	5973.0
39	6282.0
40	6914.5
41	7547.0
42	9290.5
43	11034.0
44	14663.0
45	18292.0
46	20912.0
47	23532.0
48	25877.0
49	28222.0
50	26518.5
51	24815.0
52	21523.0
53	18231.0
54	16454.5
55	14678.0
56	13771.5
57	12865.0
58	12781.5
59	12698.0
60	12218.5
61	11739.0
62	10314.0
63	8889.0
64	8061.0
65	7233.0
66	6419.5
67	5606.0
68	4828.0
69	4050.0
70	3442.0
71	2834.0
72	2583.0
73	2332.0
74	1899.0
75	1272.5
76	1079.0
77	803.0
78	527.0
79	436.0
80	345.0
81	251.5
82	158.0
83	94.5
84	31.0
85	21.5
86	12.0
87	8.5
88	5.0
89	3.0
90	1.0
91	3.5
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	256046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.182678112526656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31216652889456	24.419830811651032
2	9.188146589514291	5.730220351030675
3	3.201322612159014	2.9947743764792265
4	1.5731068861000477	1.9621474266342767
5	0.8992760702387215	1.4020918116275982
6	0.6099546604543975	1.1412011904110981
7	0.47468750782796026	1.0361419432445733
8	0.3481876706495328	0.8685939245291862
9	0.27930162070088427	0.7838435281160415
>10	4.040479947897096	31.58885512759426
>50	0.97442448836452	19.689821360224336
>100	0.09017810175095814	4.667520679877835
>500	0.0025049472708599486	0.49717628863563573
>1k	0.006262368177149871	3.217781179944229
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	2440	0.9529537661201503	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	1675	0.6541793271521523	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	1451	0.5666950469837451	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1361	0.5315451129875101	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1312	0.5124079267006709	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	751	0.29330667145747247	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	522	0.20386961717816332	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.16403302531576355	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	412	0.16090858673832045	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	340	0.1327886395413324	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	338	0.13200752989697165	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	329	0.12849253649734813	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	315	0.12302476898682269	No Hit
AGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	307	0.11990033040937956	No Hit
AACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	306	0.11950977558719918	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.10974590503268944	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	276	0.1077931309217875	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	264	0.1031064730556228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9055482218038946E-4	0.0	0.0	1.1193301203689963	0.0
2	3.9055482218038946E-4	0.0	0.0	1.5126188263046483	0.0
3	3.9055482218038946E-4	0.0	0.0	2.029713410871484	0.0
4	3.9055482218038946E-4	0.0	0.0	4.5030970997398905	0.0
5	3.9055482218038946E-4	0.0	0.0	4.841317575748108	0.0
6	3.9055482218038946E-4	0.0	0.0	6.568741554251971	0.0
7	3.9055482218038946E-4	0.0	0.0	7.8704607765792085	0.0
8	3.9055482218038946E-4	0.0	0.0	9.002288651257977	0.0
9	3.9055482218038946E-4	0.0	0.0	10.856642946970466	0.0
10	3.9055482218038946E-4	0.0	0.0	12.919553517727284	0.0
11	3.9055482218038946E-4	0.0	0.0	15.095334432094234	0.0
12	3.9055482218038946E-4	0.0	0.0	15.992048303820408	0.0
13	3.9055482218038946E-4	0.0	0.0	16.382993680822977	0.0
14	3.9055482218038946E-4	0.0	0.0	16.879388859814252	0.0
15	3.9055482218038946E-4	0.0	0.0	17.234012638354045	0.0
16	3.9055482218038946E-4	0.0	0.0	17.97020847816408	0.0
17	3.9055482218038946E-4	0.0	0.0	18.81380689407372	0.0
18	3.9055482218038946E-4	0.0	0.0	20.269014161517852	0.0
19	3.9055482218038946E-4	0.0	0.0	20.712293884692595	0.0
20	3.9055482218038946E-4	0.0	0.0	21.36022433468986	0.0
21	3.9055482218038946E-4	0.0	0.0	21.848808417237528	0.0
22	3.9055482218038946E-4	0.0	0.0	22.34910914445061	0.0
23	3.9055482218038946E-4	0.0	0.0	22.83222545948775	0.0
24	3.9055482218038946E-4	0.0	0.0	23.18684923802754	0.0
25	3.9055482218038946E-4	0.0	0.0	23.484452012528997	0.0
26	3.9055482218038946E-4	0.0	0.0	23.77932090327519	0.0
27	3.9055482218038946E-4	0.0	0.0	24.190575131031142	0.0
28	3.9055482218038946E-4	0.0	0.0	24.54871390297056	0.0
29	3.9055482218038946E-4	0.0	0.0	24.867016083047577	0.0
30	3.9055482218038946E-4	0.0	0.0	25.386844551369677	0.0
31	3.9055482218038946E-4	0.0	0.0	25.703975066980153	0.0
32	3.9055482218038946E-4	0.0	0.0	26.03126000796732	0.0
33	3.9055482218038946E-4	0.0	0.0	26.36440327128719	0.0
34	3.9055482218038946E-4	0.0	0.0	26.64521218843489	0.0
35	3.9055482218038946E-4	0.0	0.0	26.991634315708897	0.0
36	3.9055482218038946E-4	0.0	0.0	27.278692110011484	0.0
37	3.9055482218038946E-4	0.0	0.0	27.562625465736627	0.0
38	3.9055482218038946E-4	0.0	0.0	27.844996602173048	0.0
39	3.9055482218038946E-4	0.0	0.0	28.163689337072245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGGG	45	3.8198777E-10	45.000004	3
TTGATGG	25	3.882286E-5	45.0	2
CAGCGTC	25	3.882286E-5	45.0	3
GACAGTA	20	7.023006E-4	45.0	42
CGGGTCA	25	3.882286E-5	45.0	6
TAATAGT	20	7.023006E-4	45.0	21
ACTATCC	20	7.023006E-4	45.0	43
ACTGCGA	25	3.882286E-5	45.0	23
GGTAATT	20	7.023006E-4	45.0	8
GGTGTCG	20	7.023006E-4	45.0	1
ATCTACG	20	7.023006E-4	45.0	1
GTCGAAC	20	7.023006E-4	45.0	11
CCGATTC	20	7.023006E-4	45.0	39
TCCGATT	20	7.023006E-4	45.0	38
CTATCAA	25	3.882286E-5	45.0	34
TTTCGTC	20	7.023006E-4	45.0	33
GAACTTA	20	7.023006E-4	45.0	9
AGCTTCG	20	7.023006E-4	45.0	32
GACCGCG	20	7.023006E-4	45.0	34
GCAAACG	20	7.023006E-4	45.0	1
>>END_MODULE