SRR3553231_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386095
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	6113	1.583289086882762	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	2905	0.75240549605667	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	2760	0.7148499721571117	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT	2713	0.7026768023413926	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1973	0.5110141286470946	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1845	0.4778616661702431	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1242	0.3216824874707002	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1096	0.2838679599580414	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	742	0.19218068092049884	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	710	0.18389256530128595	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	709	0.18363356168818554	No Hit
AACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	640	0.16576231238425776	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	614	0.15902821844364728	No Hit
AACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	570	0.14763205946722957	No Hit
TACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	559	0.14478301972312513	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	520	0.13468187881220944	No Hit
AGCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	499	0.12924280293710097	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	493	0.12768878125849856	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	482	0.12483974151439411	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTAT	460	0.11914166202618526	No Hit
AAGCGTGGGAACGTTTTAGGACTTAACCAAATAACCAGTCCATGAATAAGC	460	0.11914166202618526	No Hit
GACCTAGGGCAAAAGAGAAACCATGTCTTCTTCTAGATATGACAAAACCAC	456	0.11810564757378365	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	442	0.11447959699037802	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTTCTTC	393	0.10178841994845829	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.0274586E-4	45.000004	31
TCCGGAT	30	2.1617416E-6	45.000004	4
TTAGGTT	20	7.0274586E-4	45.000004	21
AGGTACG	20	7.0274586E-4	45.000004	1
ATCTCCG	20	7.0274586E-4	45.000004	31
CCCCTAG	20	7.0274586E-4	45.000004	1
ACGTTAG	30	2.1617416E-6	45.000004	1
GAGTCGC	30	2.1617416E-6	45.000004	21
GCGCGAA	20	7.0274586E-4	45.000004	19
TAAACGG	65	0.0	45.000004	2
TGAACGT	20	7.0274586E-4	45.000004	12
AATGGTC	20	7.0274586E-4	45.000004	25
ATTCGGG	20	7.0274586E-4	45.000004	3
CGTCTCA	20	7.0274586E-4	45.000004	33
AATGGCG	20	7.0274586E-4	45.000004	1
CGATTGG	20	7.0274586E-4	45.000004	2
TACGGGT	20	7.0274586E-4	45.000004	4
TGACGGA	30	2.1617416E-6	45.000004	3
AACGGGT	20	7.0274586E-4	45.000004	4
ACCTATC	20	7.0274586E-4	45.000004	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content