##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386095
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420829070565535	31.0	31.0	34.0	30.0	34.0
2	31.631914425206233	31.0	31.0	34.0	30.0	34.0
3	31.808787992592496	31.0	31.0	34.0	30.0	34.0
4	35.52939561506883	37.0	35.0	37.0	33.0	37.0
5	35.28829433170593	37.0	35.0	37.0	33.0	37.0
6	35.31833357075331	37.0	35.0	37.0	33.0	37.0
7	35.47581035755449	37.0	35.0	37.0	33.0	37.0
8	35.55162589518124	37.0	35.0	37.0	35.0	37.0
9	37.253318483792846	39.0	37.0	39.0	34.0	39.0
10	36.679110063585384	38.0	35.0	39.0	32.0	39.0
11	36.760175604449685	39.0	37.0	39.0	32.0	39.0
12	36.824333389450786	39.0	37.0	39.0	32.0	39.0
13	36.794996050194904	39.0	37.0	39.0	33.0	39.0
14	37.868014348800166	39.0	37.0	41.0	33.0	41.0
15	37.889275955399576	39.0	37.0	41.0	33.0	41.0
16	37.76372136391303	39.0	37.0	41.0	33.0	41.0
17	37.73695593053523	39.0	37.0	41.0	33.0	41.0
18	37.653844261127446	39.0	37.0	41.0	33.0	41.0
19	37.637366451262	39.0	36.0	41.0	32.0	41.0
20	37.58769732837773	39.0	36.0	41.0	32.0	41.0
21	37.56698221940196	39.0	36.0	40.0	32.0	41.0
22	37.55933902277937	39.0	36.0	41.0	32.0	41.0
23	37.38106942591849	39.0	36.0	40.0	32.0	41.0
24	37.26841062432821	39.0	36.0	40.0	32.0	41.0
25	37.35601600642329	39.0	36.0	40.0	32.0	41.0
26	37.249842655305045	39.0	36.0	40.0	32.0	41.0
27	37.257972778720266	39.0	36.0	40.0	32.0	41.0
28	37.23329232442793	39.0	36.0	40.0	32.0	41.0
29	37.05972623318095	39.0	36.0	40.0	31.0	41.0
30	37.08010204742356	39.0	36.0	40.0	31.0	41.0
31	37.04115049404939	39.0	36.0	40.0	31.0	41.0
32	36.935668682578125	39.0	36.0	40.0	31.0	41.0
33	36.91681839961667	39.0	36.0	40.0	31.0	41.0
34	36.886509796811666	39.0	36.0	40.0	31.0	41.0
35	36.82981390590399	39.0	35.0	40.0	31.0	41.0
36	36.808345096414094	39.0	35.0	40.0	31.0	41.0
37	36.64460819228428	39.0	35.0	40.0	30.0	41.0
38	36.59415428845232	39.0	35.0	40.0	30.0	41.0
39	36.49137647470182	39.0	35.0	40.0	30.0	41.0
40	36.39209002965591	39.0	35.0	40.0	30.0	41.0
41	36.40528885377951	39.0	35.0	40.0	30.0	41.0
42	36.3042230539116	39.0	35.0	40.0	30.0	41.0
43	36.258009039226096	39.0	35.0	40.0	30.0	41.0
44	36.17415661948484	39.0	35.0	40.0	30.0	41.0
45	35.96436110283739	38.0	35.0	40.0	29.0	41.0
46	35.80141674976366	38.0	35.0	40.0	29.0	41.0
47	35.738416710913114	38.0	35.0	40.0	28.0	41.0
48	35.698082078244994	38.0	35.0	40.0	28.0	41.0
49	35.552229373599765	38.0	34.0	40.0	28.0	41.0
50	35.46934303733537	38.0	34.0	40.0	27.0	41.0
51	34.045229800955724	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	5.0
13	9.0
14	8.0
15	18.0
16	34.0
17	64.0
18	174.0
19	300.0
20	543.0
21	878.0
22	1187.0
23	1611.0
24	2061.0
25	2384.0
26	2651.0
27	3350.0
28	4284.0
29	5657.0
30	7243.0
31	9540.0
32	12446.0
33	16563.0
34	23218.0
35	31086.0
36	39146.0
37	55555.0
38	83957.0
39	82103.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.298087258317253	34.94502648311944	20.498581955218278	26.25830430334503
2	20.090133257358943	34.97869695282249	21.055439723384143	23.87573006643443
3	19.637912948885635	32.51971665004727	23.863815900231806	23.978554500835287
4	19.873865240420105	31.270542224064023	20.439270127818283	28.416322407697585
5	16.47573783654282	35.89505173597172	20.509719110581592	27.119491316903872
6	20.1937347025991	34.47597093979461	22.36807003457698	22.962224323029307
7	77.2273663217602	7.502298657066266	8.84186534402155	6.4284696771519965
8	78.59801344228752	5.249226226705862	6.7887437029746565	9.364016628031962
9	71.83102604281329	9.586241728072107	12.859529390434995	5.723202838679599
10	26.00189072637563	43.20257967598648	14.011318457892488	16.784211139745402
11	16.696149911291265	27.359587666247943	37.314132532148825	18.63012989031197
12	21.23441122003652	22.981649594011834	37.21001307968246	18.57392610626918
13	22.537199393931544	26.125435449824526	30.27674536059778	21.060619795646147
14	16.558100985508748	28.546342221473992	34.43608438337715	20.459472409640114
15	14.808272575402427	32.18871003250495	30.241520869216128	22.761496522876495
16	16.44362138851837	31.9602688457504	29.087141765627635	22.5089680001036
17	16.37084137323716	31.216669472539145	30.267162226913065	22.145326927310634
18	17.664305417060568	28.530283997461765	28.030148020564887	25.775262564912783
19	17.186184747277224	32.873774589155516	30.789313510923478	19.15072715264378
20	20.229477201206958	31.03433092891646	28.77297038293684	19.96322148693974
21	18.02380243204393	30.8025226951916	27.36088268431345	23.81279218845103
22	17.7477045804789	28.426941555834706	29.553089265595254	24.27226459809114
23	20.55530374648726	29.116927181134177	25.56132558049185	24.76644349188671
24	15.47235783939186	31.551820147891064	27.925251557259223	25.050570455457855
25	17.976663774459652	28.209119517217264	26.65561584584105	27.15860086248203
26	20.81534337404007	30.543260078478095	24.6952174982841	23.946179049197735
27	16.02610756420052	28.018492857975367	28.33499527318406	27.62040430464005
28	19.745140444709204	26.1091182221992	27.16947901423225	26.976262318859344
29	19.24267343529442	27.300275838847952	26.65380282054935	26.803247905308282
30	19.74902549890571	25.738743055465623	29.204211398749013	25.308020046879655
31	22.11683652986959	27.392481125111694	22.81018920213937	27.680493142879342
32	19.501159041168627	24.14328079876714	29.82582007018998	26.529740089874252
33	20.39368549191261	23.855268780999495	29.936673616596956	25.81437211049094
34	16.69381887877336	22.566984809438093	28.683873140030304	32.055323171758246
35	17.714034110775845	22.843859671842424	33.45342467527422	25.98868154210751
36	18.826195625428973	23.29374894779782	33.307346637485594	24.57270878928761
37	19.144770069542467	25.23446302075914	30.700734275243143	24.92003263445525
38	22.171745295846875	23.391652313549773	34.385319675209466	20.05128271539388
39	18.73917041142724	22.05027260130279	34.863958352219015	24.34659863505096
40	20.834768645022596	24.246105233168002	29.858972532666833	25.06015358914257
41	18.576257138787085	20.82233647159378	33.47673500045325	27.124671389165876
42	22.111915461220686	23.4087465520144	29.234773825094862	25.24456416167006
43	18.749530555951253	22.917157694349836	31.37725171266139	26.956060037037517
44	21.673681347854803	21.59986531812119	28.762351234799727	27.964102099224284
45	24.94023491627708	22.896178401688704	29.218456597469533	22.94513008456468
46	17.409963868995973	22.931402893070356	34.95098356622075	24.707649671712918
47	20.23154923011176	21.9306129320504	29.40882425309833	28.42901358473951
48	19.666921353552883	20.272471800981624	35.73990857172458	24.320698273740916
49	21.484608710291507	19.53120346028827	32.234035664797524	26.750152164622698
50	19.693857729315322	19.76223468317383	29.066680480192698	31.477227107318146
51	18.975122702961706	18.10771960268846	35.982335953586556	26.934821740763283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2314.0
1	1870.0
2	1426.0
3	974.5
4	523.0
5	504.5
6	486.0
7	475.5
8	465.0
9	555.0
10	645.0
11	683.0
12	721.0
13	805.0
14	889.0
15	1041.5
16	1194.0
17	1150.5
18	1107.0
19	1076.5
20	1046.0
21	1236.5
22	1427.0
23	1569.0
24	1711.0
25	2156.5
26	3172.0
27	3742.0
28	4602.5
29	5463.0
30	5752.0
31	6041.0
32	6656.5
33	7272.0
34	8764.0
35	10256.0
36	11069.5
37	11883.0
38	12540.5
39	13198.0
40	15125.0
41	17052.0
42	20072.0
43	23092.0
44	31076.5
45	39061.0
46	42045.5
47	45030.0
48	48017.0
49	51004.0
50	46185.5
51	41367.0
52	35050.0
53	28733.0
54	24910.0
55	21087.0
56	17345.0
57	13603.0
58	11716.5
59	9830.0
60	8865.0
61	7900.0
62	6588.0
63	5276.0
64	4308.5
65	3341.0
66	2524.0
67	1707.0
68	1500.5
69	1294.0
70	1079.5
71	865.0
72	707.5
73	550.0
74	499.0
75	338.0
76	228.0
77	158.0
78	88.0
79	61.0
80	34.0
81	49.0
82	64.0
83	37.0
84	10.0
85	8.5
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	386095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.83753913194443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.26020147247397	25.127824732709154
2	11.277323556812483	7.631937543144823
3	4.284823658729164	4.349636646771882
4	2.149081118136584	2.9087846573067817
5	1.3311886622483615	2.2522074225414848
6	0.86090578894499	1.7478559993406158
7	0.6200138005450545	1.4685818866802207
8	0.46014250374293186	1.2456071981337877
9	0.3971344579931122	1.2094247486686927
>10	3.707804015520666	27.611720102998188
>50	0.5561318615015776	12.452977902750744
>100	0.08295889646708615	5.310664450940352
>500	0.006145103442006381	1.3162296779347074
>1k	0.005376965511755583	3.7776623497438857
>5k	7.681379302507976E-4	1.588884680334689
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	6113	1.583289086882762	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	2905	0.75240549605667	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	2760	0.7148499721571117	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT	2713	0.7026768023413926	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1973	0.5110141286470946	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1845	0.4778616661702431	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1242	0.3216824874707002	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1096	0.2838679599580414	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	742	0.19218068092049884	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	710	0.18389256530128595	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	709	0.18363356168818554	No Hit
AACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	640	0.16576231238425776	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	614	0.15902821844364728	No Hit
AACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	570	0.14763205946722957	No Hit
TACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	559	0.14478301972312513	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	520	0.13468187881220944	No Hit
AGCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	499	0.12924280293710097	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	493	0.12768878125849856	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	482	0.12483974151439411	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTAT	460	0.11914166202618526	No Hit
AAGCGTGGGAACGTTTTAGGACTTAACCAAATAACCAGTCCATGAATAAGC	460	0.11914166202618526	No Hit
GACCTAGGGCAAAAGAGAAACCATGTCTTCTTCTAGATATGACAAAACCAC	456	0.11810564757378365	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	442	0.11447959699037802	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTTCTTC	393	0.10178841994845829	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5900361310040275E-4	0.0	0.0	1.7635556016006424	0.0
2	2.5900361310040275E-4	0.0	0.0	2.4377420065009905	0.0
3	2.5900361310040275E-4	0.0	0.0	3.3320814825366813	0.0
4	2.5900361310040275E-4	0.0	0.0	7.277224517282016	0.0
5	2.5900361310040275E-4	0.0	0.0	7.844442429971898	0.0
6	2.5900361310040275E-4	0.0	0.0	10.518654735233557	0.0
7	2.5900361310040275E-4	0.0	0.0	12.6054468459835	0.0
8	2.5900361310040275E-4	0.0	0.0	14.175526748598143	0.0
9	2.5900361310040275E-4	0.0	0.0	16.79767932762662	0.0
10	2.5900361310040275E-4	0.0	0.0	19.706030899131044	0.0
11	2.5900361310040275E-4	0.0	0.0	22.847485722425827	0.0
12	2.5900361310040275E-4	0.0	0.0	24.27563164506145	0.0
13	2.5900361310040275E-4	0.0	0.0	24.850619666144343	0.0
14	2.5900361310040275E-4	0.0	0.0	25.45332107382898	0.0
15	2.5900361310040275E-4	0.0	0.0	25.960968155505768	0.0
16	2.5900361310040275E-4	0.0	0.0	26.929382664888177	0.0
17	2.5900361310040275E-4	0.0	0.0	28.223623719550886	0.0
18	2.5900361310040275E-4	0.0	0.0	30.01204366800917	0.0
19	2.5900361310040275E-4	0.0	0.0	30.70824538002305	0.0
20	2.5900361310040275E-4	0.0	0.0	31.539905981688445	0.0
21	2.5900361310040275E-4	0.0	0.0	32.36768152915733	0.0
22	2.5900361310040275E-4	0.0	0.0	33.22705551742447	0.0
23	5.180072262008055E-4	0.0	0.0	34.10274673331693	0.0
24	5.180072262008055E-4	0.0	0.0	34.66582058819721	0.0
25	5.180072262008055E-4	0.0	0.0	35.14938033385566	0.0
26	5.180072262008055E-4	0.0	0.0	35.63501210841891	0.0
27	5.180072262008055E-4	0.0	0.0	36.17451663450705	0.0
28	5.180072262008055E-4	0.0	0.0	36.75002266281614	0.0
29	5.180072262008055E-4	0.0	0.0	37.296261282844895	0.0
30	5.180072262008055E-4	0.0	0.0	38.09192038228933	0.0
31	5.180072262008055E-4	0.0	0.0	38.605006539841234	0.0
32	5.180072262008055E-4	0.0	0.0	39.083386213237674	0.0
33	5.180072262008055E-4	0.0	0.0	39.552700760175604	0.0
34	5.180072262008055E-4	0.0	0.0	40.05361374791178	0.0
35	5.180072262008055E-4	0.0	0.0	40.55193669951696	0.0
36	5.180072262008055E-4	0.0	0.0	41.04456157163393	0.0
37	5.180072262008055E-4	0.0	0.0	41.513617114958755	0.0
38	5.180072262008055E-4	0.0	0.0	41.978787604087074	0.0
39	5.180072262008055E-4	0.0	0.0	42.45043318354291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.0274586E-4	45.000004	31
TCCGGAT	30	2.1617416E-6	45.000004	4
TTAGGTT	20	7.0274586E-4	45.000004	21
AGGTACG	20	7.0274586E-4	45.000004	1
ATCTCCG	20	7.0274586E-4	45.000004	31
CCCCTAG	20	7.0274586E-4	45.000004	1
ACGTTAG	30	2.1617416E-6	45.000004	1
GAGTCGC	30	2.1617416E-6	45.000004	21
GCGCGAA	20	7.0274586E-4	45.000004	19
TAAACGG	65	0.0	45.000004	2
TGAACGT	20	7.0274586E-4	45.000004	12
AATGGTC	20	7.0274586E-4	45.000004	25
ATTCGGG	20	7.0274586E-4	45.000004	3
CGTCTCA	20	7.0274586E-4	45.000004	33
AATGGCG	20	7.0274586E-4	45.000004	1
CGATTGG	20	7.0274586E-4	45.000004	2
TACGGGT	20	7.0274586E-4	45.000004	4
TGACGGA	30	2.1617416E-6	45.000004	3
AACGGGT	20	7.0274586E-4	45.000004	4
ACCTATC	20	7.0274586E-4	45.000004	9
>>END_MODULE