SRR3553230_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340954
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1466	0.4299700252820029	TruSeq Adapter, Index 3 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	812	0.23815529367597976	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	548	0.1607254937616218	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	511	0.14987358998574588	TruSeq Adapter, Index 3 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	488	0.14312781196290408	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	441	0.1293429612205752	RNA PCR Primer, Index 3 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	30	2.160994E-6	45.000004	2
CGCTACG	30	2.160994E-6	45.000004	1
GCGATAG	35	1.208573E-7	45.000004	1
ATAACCG	30	2.160994E-6	45.000004	1
ACCAACG	35	1.208573E-7	45.000004	1
GCTAACG	30	2.160994E-6	45.000004	1
ATACACG	30	2.160994E-6	45.000004	1
TCTATCG	30	2.160994E-6	45.000004	1
GGTACCA	20	7.0263E-4	45.0	9
AAATCGG	25	3.8850158E-5	45.0	2
ACACGAA	20	7.0263E-4	45.0	11
GTCGAGG	25	3.8850158E-5	45.0	2
CCTAGCG	20	7.0263E-4	45.0	1
CTATACG	20	7.0263E-4	45.0	1
GGCGTAC	20	7.0263E-4	45.0	14
ATTACGG	25	3.8850158E-5	45.0	2
GTAGGGC	45	3.8380676E-10	45.0	4
GACCCAT	20	7.0263E-4	45.0	43
CCACTAG	20	7.0263E-4	45.0	1
TTGACGA	25	3.8850158E-5	45.0	12