##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340954
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8957161376608	33.0	31.0	34.0	30.0	34.0
2	32.09672859095362	34.0	31.0	34.0	30.0	34.0
3	32.249394346451425	34.0	31.0	34.0	30.0	34.0
4	35.90299571203153	37.0	35.0	37.0	35.0	37.0
5	35.67165072121166	37.0	35.0	37.0	33.0	37.0
6	34.99427782046845	37.0	35.0	37.0	33.0	37.0
7	35.42662353279328	37.0	35.0	37.0	33.0	37.0
8	35.81489291810625	37.0	35.0	37.0	35.0	37.0
9	37.5132451885005	39.0	37.0	39.0	35.0	39.0
10	37.07600145474169	39.0	37.0	39.0	33.0	39.0
11	37.06855470239387	39.0	37.0	39.0	33.0	39.0
12	36.91979856520234	39.0	37.0	39.0	33.0	39.0
13	36.851243862808474	39.0	37.0	39.0	33.0	39.0
14	38.07258750447274	40.0	37.0	41.0	33.0	41.0
15	38.09416519530494	40.0	37.0	41.0	33.0	41.0
16	37.98928301178458	40.0	37.0	41.0	33.0	41.0
17	37.96097714061134	40.0	37.0	41.0	33.0	41.0
18	37.927186658610836	40.0	37.0	41.0	33.0	41.0
19	37.96830070918658	40.0	37.0	41.0	33.0	41.0
20	37.87057784921133	40.0	37.0	41.0	33.0	41.0
21	37.883160191697414	40.0	37.0	41.0	33.0	41.0
22	37.869692099227464	40.0	37.0	41.0	33.0	41.0
23	37.7899892654141	40.0	36.0	41.0	33.0	41.0
24	37.65182986561237	39.0	36.0	41.0	33.0	41.0
25	37.66006558069417	39.0	36.0	41.0	33.0	41.0
26	37.62684409040516	40.0	36.0	41.0	33.0	41.0
27	37.53838347694997	39.0	36.0	41.0	33.0	41.0
28	37.458228969303775	39.0	36.0	41.0	33.0	41.0
29	37.281313608287334	39.0	36.0	41.0	32.0	41.0
30	37.31178106137485	39.0	36.0	41.0	32.0	41.0
31	37.304926764314246	39.0	36.0	41.0	32.0	41.0
32	37.27589058934636	39.0	36.0	41.0	32.0	41.0
33	37.04866638901435	39.0	35.0	41.0	31.0	41.0
34	37.045135120866746	39.0	35.0	41.0	31.0	41.0
35	36.99053244719229	39.0	35.0	41.0	31.0	41.0
36	36.94815429647401	39.0	35.0	41.0	31.0	41.0
37	36.889970494553516	39.0	35.0	41.0	31.0	41.0
38	36.71452453996727	39.0	35.0	41.0	31.0	41.0
39	36.709415346351705	39.0	35.0	41.0	31.0	41.0
40	36.655636830774824	39.0	35.0	40.0	31.0	41.0
41	36.63725605213607	39.0	35.0	40.0	31.0	41.0
42	36.57644432973363	39.0	35.0	40.0	31.0	41.0
43	36.485722414167306	39.0	35.0	40.0	31.0	41.0
44	36.40059362846601	38.0	35.0	40.0	31.0	41.0
45	36.249670043466274	38.0	35.0	40.0	30.0	41.0
46	36.1098271321058	38.0	35.0	40.0	30.0	41.0
47	35.991429928964024	38.0	35.0	40.0	30.0	41.0
48	35.904617631703985	38.0	35.0	40.0	30.0	41.0
49	35.79887609472245	38.0	35.0	40.0	30.0	41.0
50	35.69889193263607	38.0	35.0	40.0	29.0	41.0
51	34.509112666224766	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	4.0
12	4.0
13	6.0
14	12.0
15	25.0
16	42.0
17	78.0
18	134.0
19	238.0
20	347.0
21	557.0
22	827.0
23	1009.0
24	1319.0
25	1668.0
26	2159.0
27	2637.0
28	3231.0
29	4177.0
30	5517.0
31	7353.0
32	10015.0
33	13658.0
34	22748.0
35	27659.0
36	31378.0
37	46294.0
38	71038.0
39	86791.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.774356658082908	31.9180886571209	21.250960540131516	26.056594144664675
2	24.451392269924977	29.57671709380151	20.209764367040712	25.762126269232798
3	21.374437607419182	31.232365656364202	21.279996715099397	26.113200021117215
4	22.144336186113083	30.776292403080767	19.737266610745145	27.34210480006101
5	18.760301976219665	34.077324213823566	17.9226523226007	29.239721487356064
6	20.36462396686943	38.33420344093338	19.128680115206155	22.17249247699103
7	85.20474902772807	5.511007349965098	5.220645600286256	4.0635980220205665
8	86.50375123917009	4.561025827530987	4.167131049936355	4.768091883362565
9	79.40719275914053	7.78756078532588	7.873496131442952	4.93175032409064
10	37.7754770438241	31.84476498295958	14.001595523149751	16.378162450066576
11	29.155839204115512	28.949946327070514	23.169401150888387	18.724813317925584
12	28.51235063967573	23.487625896748536	28.495926136663595	19.504097326912134
13	26.415880148055166	24.942660886805847	27.28872516527156	21.352733799867433
14	21.873625181109475	27.559729464971817	28.320242613373065	22.246402740545644
15	21.497914674706852	28.684807921303168	28.866357338526605	20.95092006546338
16	24.17686843386498	26.949383201252953	26.676325838676185	22.19742252620588
17	23.44568475512826	27.094564076092375	27.034438663280092	22.425312505499274
18	24.80774532634901	26.09501574992521	25.993829079582582	23.1034098441432
19	24.453152038104847	29.276090029740082	25.768578752558994	20.502179179596077
20	25.003372889011423	28.234893856649286	25.60316054365105	21.158572710688244
21	24.99164110114561	27.83073376467207	25.535116174029344	21.64250896015298
22	23.153856531966188	26.345196126163646	26.450488922259307	24.050458419610855
23	23.635153129161118	26.809188336256508	25.638355907248485	23.917302627333893
24	23.06410835479273	27.686139479225936	26.108800600667536	23.1409515653138
25	23.769775394921307	27.0461704511459	25.11805111539973	24.066003038533058
26	24.382174721516687	27.227719868369338	25.091361298005012	23.298744112108967
27	22.484558034221624	25.20809258726984	27.476140476427908	24.831208902080633
28	22.69690339459282	27.567355127084593	28.228148078626443	21.507593399696145
29	24.493626706241898	27.96154319937587	24.32762190794066	23.217208186441574
30	24.743220493087044	27.219214322166625	26.21966599599946	21.81789918874687
31	25.961859957648247	27.526000574857605	24.18860022173079	22.323539245763357
32	25.848354910046517	26.32319902391525	25.053819576834414	22.774626489203822
33	23.94927174926823	27.13298568135291	25.81814555629205	23.099597013086807
34	21.856027499310756	26.07301864767681	28.265103210403748	23.805850642608682
35	24.650832663643776	25.741302345770983	27.56940818996111	22.038456800624132
36	24.508584735770807	28.450465458683578	26.364553576142235	20.67639622940338
37	24.670776703015658	28.43433425036809	25.010705256427553	21.884183790188704
38	24.327915202637307	27.904350733530038	25.87856426380098	21.889169800031677
39	23.23979187808326	26.131970881702514	26.97783278682755	23.650404453386674
40	24.140793186177607	25.413105580224897	27.47144776128158	22.974653472315914
41	22.192729811059557	24.716530675692322	28.745519923508745	24.34521958973938
42	23.761856438111888	25.079629510139196	27.06112848067481	24.097385571074103
43	22.84384403761211	23.255923086398752	28.20409791350153	25.69613496248761
44	23.722261654064773	23.37822697489984	27.01156167694176	25.88794969409363
45	25.67296468145263	23.763909500988404	27.828973996492195	22.734151821066774
46	21.823471787983127	26.351648609489843	28.434040955671442	23.390838646855588
47	22.734445115763418	26.114079905207156	27.116561178340774	24.034913800688656
48	22.0809845316377	24.628542266698734	29.96445268276659	23.32602051889698
49	24.059843849903505	23.899118356141884	28.424362230682142	23.616675563272466
50	22.68311854385049	23.353590220381633	27.623374414143843	26.339916821624033
51	22.465787173636325	23.582360083764968	29.24881362295207	24.70303911964664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1014.0
1	846.5
2	679.0
3	471.0
4	263.0
5	247.0
6	231.0
7	257.0
8	283.0
9	301.0
10	319.0
11	358.5
12	398.0
13	482.5
14	567.0
15	546.5
16	526.0
17	607.0
18	688.0
19	777.5
20	867.0
21	915.5
22	964.0
23	1348.5
24	1733.0
25	1758.5
26	2563.0
27	3342.0
28	4075.5
29	4809.0
30	5635.5
31	6462.0
32	6544.5
33	6627.0
34	7652.0
35	8677.0
36	10161.0
37	11645.0
38	12027.5
39	12410.0
40	12916.0
41	13422.0
42	14507.5
43	15593.0
44	18409.5
45	21226.0
46	23913.0
47	26600.0
48	28112.0
49	29624.0
50	29142.0
51	28660.0
52	25989.5
53	23319.0
54	22098.5
55	20878.0
56	19180.0
57	17482.0
58	17186.0
59	16890.0
60	16716.0
61	16542.0
62	14675.0
63	12808.0
64	11872.5
65	10937.0
66	9109.0
67	7281.0
68	6343.0
69	5405.0
70	4372.0
71	3339.0
72	2899.5
73	2460.0
74	2025.5
75	1361.5
76	1132.0
77	931.0
78	730.0
79	546.5
80	363.0
81	239.5
82	116.0
83	112.5
84	109.0
85	79.5
86	50.0
87	64.0
88	78.0
89	48.0
90	18.0
91	12.0
92	6.0
93	6.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.54209788663672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77699071271572	22.68153375076446
2	8.61999895728681	5.093057059577468
3	2.81801165665285	2.4974992861956538
4	1.4179999262833107	1.6756277050202086
5	0.9729998833888279	1.4372228899379436
6	0.6609999200544889	1.1716394604785252
7	0.542999934316486	1.1228950048410455
8	0.4709999430257665	1.1131461137174008
9	0.41699994955784225	1.1087147995704314
>10	6.562999206110595	46.45169367696644
>50	0.6959999158087726	12.66676377862547
>100	0.038999995282388115	1.9943867870770002
>500	0.0029999996371067785	0.5527325845979955
>1k	9.99999879035593E-4	0.43308710262996336
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1466	0.4299700252820029	TruSeq Adapter, Index 3 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	812	0.23815529367597976	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	548	0.1607254937616218	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	511	0.14987358998574588	TruSeq Adapter, Index 3 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	488	0.14312781196290408	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	441	0.1293429612205752	RNA PCR Primer, Index 3 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47308434568886126	0.0
2	0.0	0.0	0.0	0.7226781325340075	0.0
3	0.0	0.0	0.0	1.0156795344826575	0.0
4	0.0	0.0	0.0	2.1205206567454846	0.0
5	0.0	0.0	0.0	2.3082292625984735	0.0
6	0.0	0.0	0.0	3.2036579714565603	0.0
7	0.0	0.0	0.0	3.870903406324607	0.0
8	0.0	0.0	0.0	4.511752318494577	0.0
9	0.0	0.0	0.0	5.3857705144975565	0.0
10	0.0	0.0	0.0	6.6061697472386305	0.0
11	0.0	0.0	0.0	7.699279081635646	0.0
12	0.0	0.0	0.0	8.198759950022584	0.0
13	0.0	0.0	0.0	8.457152577767088	0.0
14	0.0	0.0	0.0	8.725810519894178	0.0
15	0.0	0.0	0.0	8.99945447186424	0.0
16	0.0	0.0	0.0	9.46080702968729	0.0
17	0.0	0.0	0.0	9.999589387424697	0.0
18	0.0	0.0	0.0	10.768314787332015	0.0
19	2.9329469664529526E-4	0.0	0.0	11.088885890765324	0.0
20	2.9329469664529526E-4	0.0	0.0	11.486300204719699	0.0
21	2.9329469664529526E-4	0.0	0.0	11.903365263349308	0.0
22	2.9329469664529526E-4	0.0	0.0	12.315737606832593	0.0
23	2.9329469664529526E-4	0.0	0.0	12.735149023035365	0.0
24	2.9329469664529526E-4	0.0	0.0	13.056306715861965	0.0
25	2.9329469664529526E-4	0.0	0.0	13.34520199205758	0.0
26	2.9329469664529526E-4	0.0	0.0	13.61444652357796	0.0
27	2.9329469664529526E-4	0.0	0.0	13.947629298967016	0.0
28	2.9329469664529526E-4	0.0	0.0	14.24356364788212	0.0
29	2.9329469664529526E-4	0.0	0.0	14.545950480123418	0.0
30	5.865893932905905E-4	0.0	0.0	14.95920270769664	0.0
31	5.865893932905905E-4	0.0	0.0	15.284466526276272	0.0
32	5.865893932905905E-4	0.0	0.0	15.574828275955115	0.0
33	5.865893932905905E-4	0.0	0.0	15.916516597546883	0.0
34	8.798840899358858E-4	0.0	0.0	16.202772221472692	0.0
35	8.798840899358858E-4	0.0	0.0	16.506332232500572	0.0
36	8.798840899358858E-4	0.0	0.0	16.782909131437087	0.0
37	8.798840899358858E-4	0.0	0.0	17.07737700686896	0.0
38	8.798840899358858E-4	0.0	0.0	17.4085067193815	0.0
39	8.798840899358858E-4	0.0	0.0	17.749021862186687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	30	2.160994E-6	45.000004	2
CGCTACG	30	2.160994E-6	45.000004	1
GCGATAG	35	1.208573E-7	45.000004	1
ATAACCG	30	2.160994E-6	45.000004	1
ACCAACG	35	1.208573E-7	45.000004	1
GCTAACG	30	2.160994E-6	45.000004	1
ATACACG	30	2.160994E-6	45.000004	1
TCTATCG	30	2.160994E-6	45.000004	1
GGTACCA	20	7.0263E-4	45.0	9
AAATCGG	25	3.8850158E-5	45.0	2
ACACGAA	20	7.0263E-4	45.0	11
GTCGAGG	25	3.8850158E-5	45.0	2
CCTAGCG	20	7.0263E-4	45.0	1
CTATACG	20	7.0263E-4	45.0	1
GGCGTAC	20	7.0263E-4	45.0	14
ATTACGG	25	3.8850158E-5	45.0	2
GTAGGGC	45	3.8380676E-10	45.0	4
GACCCAT	20	7.0263E-4	45.0	43
CCACTAG	20	7.0263E-4	45.0	1
TTGACGA	25	3.8850158E-5	45.0	12
>>END_MODULE