SRR3553223_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455865
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	2224	0.48786373158720236	TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1058	0.2320862536057824	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	515	0.11297204216160486	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	511	0.11209458940695163	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	481	0.1055136937470523	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	469	0.10288133548309258	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGA	25	3.8870887E-5	45.000004	10
TCAGGTT	25	3.8870887E-5	45.000004	11
ATATCGG	25	3.8870887E-5	45.000004	2
GTTCGGT	25	3.8870887E-5	45.000004	12
TAACACG	35	1.2097735E-7	45.000004	1
GGAACCG	25	3.8870887E-5	45.000004	9
CGTACAG	25	3.8870887E-5	45.000004	1
ATAGTCG	25	3.8870887E-5	45.000004	1
GGCGATA	25	3.8870887E-5	45.000004	36
TTACCGG	25	3.8870887E-5	45.000004	2
AATCGCG	25	3.8870887E-5	45.000004	1
TGCGTAG	25	3.8870887E-5	45.000004	1
TACCGGT	35	1.2097735E-7	45.000004	3
TCGCATA	20	7.028803E-4	45.0	39
ACTGCGT	20	7.028803E-4	45.0	16
TCCGCTA	20	7.028803E-4	45.0	28
CCCTCGA	20	7.028803E-4	45.0	30
ATAGGCG	40	6.7993824E-9	45.0	17
ACGTTAC	20	7.028803E-4	45.0	41
CGGCCTA	20	7.028803E-4	45.0	20