##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455865
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91210775119827	33.0	31.0	34.0	30.0	34.0
2	32.11679334890812	34.0	31.0	34.0	30.0	34.0
3	32.26430412512476	34.0	31.0	34.0	30.0	34.0
4	35.90692419905016	37.0	35.0	37.0	35.0	37.0
5	35.6917047810207	37.0	35.0	37.0	33.0	37.0
6	35.71670121636888	37.0	35.0	37.0	33.0	37.0
7	35.78598269224442	37.0	35.0	37.0	35.0	37.0
8	35.830313799041384	37.0	35.0	37.0	35.0	37.0
9	37.51955513145339	39.0	37.0	39.0	35.0	39.0
10	37.091737685499	39.0	37.0	39.0	33.0	39.0
11	37.07477871738344	39.0	37.0	39.0	33.0	39.0
12	36.95698507233501	39.0	37.0	39.0	33.0	39.0
13	36.90659296063527	39.0	37.0	39.0	33.0	39.0
14	38.09487677272877	40.0	37.0	41.0	33.0	41.0
15	38.13620041020916	40.0	37.0	41.0	33.0	41.0
16	38.03619711976133	40.0	37.0	41.0	33.0	41.0
17	37.99979818586643	40.0	37.0	41.0	33.0	41.0
18	37.93129764294254	40.0	37.0	41.0	33.0	41.0
19	38.00373575510294	40.0	37.0	41.0	33.0	41.0
20	37.92784705998486	40.0	37.0	41.0	33.0	41.0
21	37.87944895967008	40.0	37.0	41.0	33.0	41.0
22	37.87274302699264	40.0	37.0	41.0	33.0	41.0
23	37.74409309773727	40.0	36.0	41.0	33.0	41.0
24	37.62395884746581	40.0	36.0	41.0	33.0	41.0
25	37.635495157557614	40.0	36.0	41.0	33.0	41.0
26	37.5776973446086	39.0	36.0	41.0	33.0	41.0
27	37.5292027244908	39.0	36.0	41.0	33.0	41.0
28	37.45375275575006	39.0	36.0	41.0	33.0	41.0
29	37.32059710659954	39.0	36.0	41.0	32.0	41.0
30	37.33266427560791	39.0	36.0	41.0	32.0	41.0
31	37.24228225461485	39.0	36.0	41.0	32.0	41.0
32	37.188634793195355	39.0	35.0	41.0	32.0	41.0
33	37.120393098834086	39.0	35.0	41.0	32.0	41.0
34	37.009250545665935	39.0	35.0	41.0	31.0	41.0
35	36.93100588990161	39.0	35.0	41.0	31.0	41.0
36	36.85255722637184	39.0	35.0	41.0	31.0	41.0
37	36.80020839502923	39.0	35.0	41.0	31.0	41.0
38	36.70361181490134	39.0	35.0	41.0	31.0	41.0
39	36.66396630581422	39.0	35.0	41.0	31.0	41.0
40	36.54798021344038	39.0	35.0	41.0	30.0	41.0
41	36.448137058120274	39.0	35.0	40.0	30.0	41.0
42	36.364809757274635	39.0	35.0	40.0	30.0	41.0
43	36.28683272460049	38.0	35.0	40.0	30.0	41.0
44	36.16930889627412	38.0	35.0	40.0	30.0	41.0
45	35.939580796946466	38.0	35.0	40.0	30.0	41.0
46	35.78917881390324	38.0	35.0	40.0	29.0	41.0
47	35.61771796474834	38.0	34.0	40.0	28.0	41.0
48	35.537663562677544	38.0	34.0	40.0	28.0	41.0
49	35.39553157184693	38.0	34.0	40.0	28.0	41.0
50	35.297401643030284	37.0	34.0	40.0	28.0	41.0
51	33.989650444758865	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	8.0
12	6.0
13	7.0
14	8.0
15	20.0
16	55.0
17	77.0
18	156.0
19	326.0
20	561.0
21	828.0
22	1142.0
23	1600.0
24	2023.0
25	2436.0
26	3025.0
27	3710.0
28	4788.0
29	6221.0
30	7777.0
31	10216.0
32	13262.0
33	18324.0
34	29745.0
35	36826.0
36	40977.0
37	60296.0
38	93812.0
39	117598.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.65669660974192	31.147817884680773	22.068375505906353	27.127109999670957
2	24.645454246322927	30.000109681594335	20.188652342250446	25.1657837298323
3	21.653779079332697	30.056705384269467	20.671690083687054	27.617825452710782
4	20.308424643260615	32.65440426442039	19.97806368113367	27.05910741118533
5	18.71321553530102	34.137738146161695	16.998234126331262	30.15081219220603
6	20.051989075713205	38.172704638434624	18.61165037894991	23.163655906902264
7	85.96185274149146	5.3136345189913685	4.871178967457471	3.8533337720597105
8	87.37060313908724	4.123150494115582	4.0718195079683674	4.43442685882882
9	79.74926787535783	8.171278777708313	7.578778805128711	4.50067454180514
10	36.43885799523982	34.75656170138089	11.966481304772246	16.838098998607045
11	27.973851907911335	28.711350948197385	24.108233797286477	19.206563346604806
12	28.06773935265923	23.931207704035184	29.243087317517247	18.757965625788337
13	26.781174251148915	24.206289142618978	26.60678051616158	22.405756090070525
14	21.373871650598314	27.978458534873262	27.892029438539918	22.755640375988506
15	19.986399482302875	29.026575850306557	28.46083818674388	22.526186480646682
16	23.689250106939554	28.096695293562785	26.05464337029603	22.159411229201627
17	22.911168876750793	27.524376734340212	26.90292082085705	22.661533568051944
18	24.98919636295833	26.036874952014305	26.3878560538756	22.586072631151765
19	23.29549318328891	30.480076338389654	25.207462735678327	21.016967742643107
20	25.823434569444903	27.92252092176412	24.990073815712986	21.263970693077994
21	25.41190922751253	27.558816754960354	25.64004694372237	21.389227073804744
22	23.520998541234796	26.68684808002369	24.91351606286949	24.878637315872023
23	22.519824948175447	27.842892084279335	25.12849198775953	24.508790979785683
24	22.221491011593344	27.260921544755572	26.816052998146382	23.7015344455047
25	23.751110526142607	28.932688405558665	23.21718052493611	24.099020543362617
26	23.445976330711943	27.56013293409233	24.824893334649513	24.168997400546214
27	21.242253737400326	27.26816052998146	25.454465686113213	26.035120046504996
28	21.48092088666601	28.706744321235455	26.60941287442554	23.202921917672995
29	23.596459478134975	28.366073289241335	23.597117567700966	24.440349664922728
30	24.396915753567395	28.567009970056922	24.88850865936187	22.14756561701381
31	24.093317100457373	28.272843934059427	24.329351891459094	23.30448707402411
32	24.74767749224003	26.552159082184417	24.234806357145207	24.465357068430347
33	24.743509591655425	26.219165761793512	25.09098088249811	23.946343764052955
34	21.4008533228039	28.701260241518874	26.2419795334145	23.65590690226273
35	23.315455233457275	27.120090377633726	26.250095971395037	23.314358417513954
36	24.281530716330494	29.942417162975882	25.031533458370355	20.744518662323273
37	22.31735272503921	30.702949338071576	23.047832143288037	23.931865793601176
38	22.478804031895407	28.80523839294528	25.780658747655554	22.935298827503754
39	23.48765533655797	25.852171147159797	24.791769493161354	25.868404023120878
40	24.78825968214274	27.42346966755509	23.654810086319415	24.13346056398276
41	22.082414749980806	25.34171300714027	25.900650411854386	26.675221831024537
42	23.042348063571453	27.29141302797978	24.436839853904115	25.229399054544654
43	22.2504469524969	25.707172079453343	26.41922498985445	25.6231559781953
44	24.43047832143288	24.40327728603863	24.34624285698617	26.82000153554232
45	24.41446480866046	24.635802266021738	27.392978184330886	23.556754740986914
46	22.013973435117855	26.537900474921305	26.880326412424733	24.56779967753611
47	21.952113015914797	27.49256907198403	26.12154914283834	24.43376876926283
48	21.654656532087348	25.667906068682615	28.544415561624604	24.13302183760543
49	23.000230331348096	24.889166748927863	27.589966327750542	24.520636591973503
50	22.143178353240543	24.114156603380387	27.309181446261505	26.43348359711757
51	21.85493512333695	24.055586632007284	28.19694427078192	25.892533973873842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1418.0
1	1149.0
2	880.0
3	616.0
4	352.0
5	337.5
6	323.0
7	325.5
8	328.0
9	381.0
10	434.0
11	432.0
12	430.0
13	592.0
14	754.0
15	842.0
16	930.0
17	824.0
18	718.0
19	798.5
20	879.0
21	973.5
22	1068.0
23	1288.5
24	1509.0
25	1772.5
26	3045.5
27	4055.0
28	4309.5
29	4564.0
30	6030.5
31	7497.0
32	8565.5
33	9634.0
34	10722.0
35	11810.0
36	13180.5
37	14551.0
38	14816.0
39	15081.0
40	16766.5
41	18452.0
42	20252.0
43	22052.0
44	25025.0
45	27998.0
46	31164.0
47	34330.0
48	38580.5
49	42831.0
50	41137.0
51	39443.0
52	36881.0
53	34319.0
54	31488.5
55	28658.0
56	27234.5
57	25811.0
58	23653.0
59	21495.0
60	22018.5
61	22542.0
62	20296.5
63	18051.0
64	15231.0
65	12411.0
66	10775.5
67	9140.0
68	7761.0
69	6382.0
70	5093.5
71	3805.0
72	3567.0
73	3329.0
74	3005.5
75	1935.5
76	1189.0
77	1019.5
78	850.0
79	701.0
80	552.0
81	363.0
82	174.0
83	110.5
84	47.0
85	36.0
86	25.0
87	26.0
88	27.0
89	17.5
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.84091242614842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74360973548275	19.83124898441121
2	9.538333602427334	4.929584866234271
3	3.2444409648595998	2.515179445342362
4	1.5781476804382684	1.6312310402293824
5	0.9145522813388407	1.1816432705605615
6	0.596225045333464	0.9244199509643048
7	0.45035176833339546	0.8146250424525048
8	0.3471438995755589	0.7176412086562943
9	0.3164379123059368	0.7359339942189852
>10	4.411365044847386	28.348548681278935
>50	1.6239827944354908	29.15545297891788
>100	0.23199754206221299	8.264983869614243
>500	0.0017058642798692132	0.22613598896568474
>1k	0.0017058642798692132	0.7233706781533893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	2224	0.48786373158720236	TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1058	0.2320862536057824	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	515	0.11297204216160486	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	511	0.11209458940695163	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	481	0.1055136937470523	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	469	0.10288133548309258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.387263773266208E-4	0.0	0.0	0.5718798328452502	0.0
2	4.387263773266208E-4	0.0	0.0	0.7752295087361389	0.0
3	4.387263773266208E-4	0.0	0.0	1.0310069867175589	0.0
4	4.387263773266208E-4	0.0	0.0	2.1657727616728635	0.0
5	4.387263773266208E-4	0.0	0.0	2.3489410242067277	0.0
6	4.387263773266208E-4	0.0	0.0	3.2961512728549023	0.0
7	4.387263773266208E-4	0.0	0.0	3.9943843023702192	0.0
8	4.387263773266208E-4	0.0	0.0	4.632292455003126	0.0
9	4.387263773266208E-4	0.0	0.0	5.4575367707544995	0.0
10	4.387263773266208E-4	0.0	0.0	6.687506169589681	0.0
11	4.387263773266208E-4	0.0	0.0	7.817007228017067	0.0
12	4.387263773266208E-4	0.0	0.0	8.420475360029833	0.0
13	4.387263773266208E-4	0.0	0.0	8.659800598861505	0.0
14	4.387263773266208E-4	0.0	0.0	8.950237460651728	0.0
15	4.387263773266208E-4	0.0	0.0	9.20140831167122	0.0
16	4.387263773266208E-4	0.0	0.0	9.687736500937778	0.0
17	4.387263773266208E-4	0.0	0.0	10.303269608327026	0.0
18	4.387263773266208E-4	0.0	0.0	11.186864532262842	0.0
19	4.387263773266208E-4	0.0	0.0	11.523806390049685	0.0
20	4.387263773266208E-4	0.0	0.0	11.986882081317933	0.0
21	4.387263773266208E-4	0.0	0.0	12.391826527590405	0.0
22	4.387263773266208E-4	0.0	0.0	12.825288188389106	0.0
23	4.387263773266208E-4	0.0	0.0	13.260943481074442	0.0
24	4.387263773266208E-4	0.0	0.0	13.59218189595604	0.0
25	4.387263773266208E-4	0.0	0.0	13.891173922104132	0.0
26	4.387263773266208E-4	0.0	0.0	14.136860693407039	0.0
27	4.387263773266208E-4	0.0	0.0	14.461956939006065	0.0
28	4.387263773266208E-4	0.0	0.0	14.754368069494259	0.0
29	4.387263773266208E-4	0.0	0.0	15.060160354490913	0.0
30	4.387263773266208E-4	0.0	0.0	15.515997060533271	0.0
31	4.387263773266208E-4	0.0	0.0	15.802485384927556	0.0
32	4.387263773266208E-4	0.0	0.0	16.11025193862218	0.0
33	4.387263773266208E-4	0.0	0.0	16.405295427374334	0.0
34	4.387263773266208E-4	0.0	0.0	16.714378160200937	0.0
35	4.387263773266208E-4	0.0	0.0	17.034867778838034	0.0
36	4.387263773266208E-4	0.0	0.0	17.311923486119795	0.0
37	4.387263773266208E-4	0.0	0.0	17.594901999495466	0.0
38	4.387263773266208E-4	0.0	0.0	17.92657914075439	0.0
39	4.387263773266208E-4	0.0	0.0	18.273611705219746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGA	25	3.8870887E-5	45.000004	10
TCAGGTT	25	3.8870887E-5	45.000004	11
ATATCGG	25	3.8870887E-5	45.000004	2
GTTCGGT	25	3.8870887E-5	45.000004	12
TAACACG	35	1.2097735E-7	45.000004	1
GGAACCG	25	3.8870887E-5	45.000004	9
CGTACAG	25	3.8870887E-5	45.000004	1
ATAGTCG	25	3.8870887E-5	45.000004	1
GGCGATA	25	3.8870887E-5	45.000004	36
TTACCGG	25	3.8870887E-5	45.000004	2
AATCGCG	25	3.8870887E-5	45.000004	1
TGCGTAG	25	3.8870887E-5	45.000004	1
TACCGGT	35	1.2097735E-7	45.000004	3
TCGCATA	20	7.028803E-4	45.0	39
ACTGCGT	20	7.028803E-4	45.0	16
TCCGCTA	20	7.028803E-4	45.0	28
CCCTCGA	20	7.028803E-4	45.0	30
ATAGGCG	40	6.7993824E-9	45.0	17
ACGTTAC	20	7.028803E-4	45.0	41
CGGCCTA	20	7.028803E-4	45.0	20
>>END_MODULE