SRR3553221_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723859
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	3362	0.46445509415507713	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	2112	0.2917695297012263	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT	2019	0.2789217237058599	No Hit
AATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	1921	0.2653831754526779	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1128	0.155831453363155	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	914	0.1262676847286557	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	30	2.1643827E-6	45.000004	27
ATTACGA	20	7.031553E-4	45.000004	37
CGCGACA	20	7.031553E-4	45.000004	23
GTCACGA	20	7.031553E-4	45.000004	45
ACGTCCC	20	7.031553E-4	45.000004	39
CATAACG	20	7.031553E-4	45.000004	1
CACCGTA	20	7.031553E-4	45.000004	25
ATACGAG	30	2.1643827E-6	45.000004	1
TATACGG	80	0.0	45.000004	2
CAATCGT	20	7.031553E-4	45.000004	25
CGATAGC	20	7.031553E-4	45.000004	16
GCGTATT	20	7.031553E-4	45.000004	11
TCGAAAC	20	7.031553E-4	45.000004	39
ATCGATG	30	2.1643827E-6	45.000004	1
TACGTAG	60	0.0	45.000004	1
ACGAACT	20	7.031553E-4	45.000004	38
GCGAATG	35	1.2111013E-7	45.0	1
ACGCATC	25	3.8893693E-5	45.0	37
ACGTTGC	25	3.8893693E-5	45.0	18
ACGTTAG	25	3.8893693E-5	45.0	1