##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553221_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 723859 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.857607628004903 33.0 31.0 34.0 30.0 34.0 2 32.06856860244882 33.0 31.0 34.0 30.0 34.0 3 32.21224022910539 34.0 31.0 34.0 30.0 34.0 4 35.861803196478874 37.0 35.0 37.0 35.0 37.0 5 35.64329793509509 37.0 35.0 37.0 33.0 37.0 6 35.66059826568434 37.0 35.0 37.0 33.0 37.0 7 35.754036352383544 37.0 35.0 37.0 35.0 37.0 8 35.77884228834621 37.0 35.0 37.0 35.0 37.0 9 37.478785233035715 39.0 37.0 39.0 35.0 39.0 10 37.03914712671943 39.0 37.0 39.0 33.0 39.0 11 37.02692927766319 39.0 37.0 39.0 33.0 39.0 12 36.92060332191767 39.0 37.0 39.0 33.0 39.0 13 36.87941297959962 39.0 37.0 39.0 33.0 39.0 14 38.07933036682558 40.0 37.0 41.0 33.0 41.0 15 38.097858837149225 40.0 37.0 41.0 33.0 41.0 16 38.02182883683148 40.0 37.0 41.0 33.0 41.0 17 37.98346639331693 40.0 37.0 41.0 33.0 41.0 18 37.9522379358411 40.0 37.0 41.0 33.0 41.0 19 37.96281734426179 40.0 37.0 41.0 33.0 41.0 20 37.89909222652478 40.0 37.0 41.0 33.0 41.0 21 37.88637842452743 40.0 37.0 41.0 33.0 41.0 22 37.83143125940273 40.0 37.0 41.0 33.0 41.0 23 37.70559736081198 40.0 36.0 41.0 33.0 41.0 24 37.54133332596542 39.0 36.0 41.0 32.0 41.0 25 37.60397259687315 39.0 36.0 41.0 33.0 41.0 26 37.566705670579495 39.0 36.0 41.0 33.0 41.0 27 37.46396743012106 39.0 36.0 41.0 32.0 41.0 28 37.441794603645185 39.0 36.0 41.0 32.0 41.0 29 37.278033429162306 39.0 36.0 41.0 32.0 41.0 30 37.28020926727443 39.0 36.0 41.0 32.0 41.0 31 37.253235782106735 39.0 36.0 41.0 32.0 41.0 32 37.20719504765431 39.0 36.0 41.0 32.0 41.0 33 37.153181766062175 39.0 35.0 41.0 31.0 41.0 34 37.08634416371144 39.0 35.0 41.0 31.0 41.0 35 36.98683721553507 39.0 35.0 41.0 31.0 41.0 36 36.897564304650494 39.0 35.0 41.0 31.0 41.0 37 36.795704688344 39.0 35.0 41.0 31.0 41.0 38 36.74988637289859 39.0 35.0 41.0 31.0 41.0 39 36.67405945080465 39.0 35.0 41.0 31.0 41.0 40 36.568531993109154 39.0 35.0 40.0 30.0 41.0 41 36.46497315084844 39.0 35.0 40.0 30.0 41.0 42 36.38032406863768 39.0 35.0 40.0 30.0 41.0 43 36.25178660484984 38.0 35.0 40.0 30.0 41.0 44 36.17425216789458 38.0 35.0 40.0 30.0 41.0 45 35.93892318807945 38.0 35.0 40.0 29.0 41.0 46 35.76260984528755 38.0 34.0 40.0 29.0 41.0 47 35.59911253434716 38.0 34.0 40.0 28.0 41.0 48 35.494572838080344 38.0 34.0 40.0 28.0 41.0 49 35.32282944606615 37.0 34.0 40.0 28.0 41.0 50 35.23998734560184 37.0 34.0 40.0 28.0 41.0 51 33.884782809911876 36.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 1.0 10 4.0 11 5.0 12 12.0 13 11.0 14 20.0 15 33.0 16 69.0 17 141.0 18 251.0 19 458.0 20 793.0 21 1245.0 22 1759.0 23 2408.0 24 3067.0 25 3895.0 26 4803.0 27 6003.0 28 7794.0 29 9972.0 30 12919.0 31 16585.0 32 21784.0 33 29955.0 34 46423.0 35 56529.0 36 67343.0 37 98672.0 38 149964.0 39 180889.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.175338014723863 32.053341880117536 20.746029268130947 27.025290837027654 2 23.70309687383869 30.645747307141306 20.12864383809554 25.522511980924463 3 21.484570890187175 30.34803739402287 21.054929205825996 27.112462509963958 4 21.704641373527163 30.57487715148945 19.699416599089048 28.021064875894336 5 18.285329048889356 34.04696218462435 17.77031162146219 29.8973971450241 6 20.839141324484466 36.9808208504695 19.04334960261598 23.13668822243006 7 84.97525070490248 5.384750345025758 5.232096306048554 4.407902644023214 8 86.29650249565177 4.16241284559562 3.9507694178009802 5.590315240951622 9 78.8500246594986 7.9635674903537845 8.113320411848164 5.0730874382994475 10 37.01176610361963 31.675229568189383 13.183644881116349 18.129359447074638 11 28.089586507869623 27.900461277679767 23.390052482596747 20.619899731853856 12 27.15970928039853 23.021196116923324 29.514864082645932 20.304230520032217 13 25.35245123705031 24.633388546664474 27.007745983679143 23.006414232606073 14 21.28370304161446 26.303741474513682 29.315101421685714 23.09745406218614 15 20.763297824576334 28.638864751284437 27.268017666423987 23.329819757715246 16 22.40767884353168 26.21960906751177 27.14216442705002 24.23054766190653 17 22.31788235001568 26.16103412404902 27.429513206301227 24.091570319634073 18 23.70282057693556 25.823813753783543 26.664723378448013 23.808642290832882 19 22.647228258542064 29.266749463638636 26.233285764216514 21.85273651360279 20 24.805107071957384 27.70415232800863 25.251326570506134 22.23941402952785 21 24.968398541704946 26.917811341711577 25.47084446003987 22.64294565654361 22 23.668145315593232 25.10005401604456 25.773113272059888 25.45868739630232 23 23.980360816125792 27.128625878796836 24.372426121661817 24.518587183415555 24 21.71127249920219 28.023965993377164 26.63640294587758 23.628358561543063 25 22.98790234009662 26.77510399124691 24.37270241856494 25.864291250091526 26 24.906093590049995 25.917202107040183 24.50504863516237 24.671655667747448 27 21.366039518746057 25.890125010533822 26.858131210636326 25.885704260083802 28 22.239690326430978 26.569124649966362 26.791129211628235 24.40005581197443 29 23.567296945952183 27.719072360777446 24.885923916121786 23.827706777148588 30 23.50678792416755 26.893773511139603 24.46553817801533 25.133900386677517 31 26.058113527634525 26.329989680310668 23.435779620064128 24.176117171990676 32 23.96019114219758 26.227207232347737 25.128512597066553 24.684089028388126 33 23.81292489283134 24.93137475668604 25.723103532594056 25.532596817888564 34 23.431911463420363 25.671159714806336 25.718820930595598 25.178107891177703 35 24.004674943600897 26.285505878907355 25.71232795337213 23.997491224119614 36 25.20090238568561 27.394423499604205 26.03227976719223 21.372394347517957 37 23.64258785205406 28.385500491117742 25.07394395869914 22.897967698129055 38 24.178741992570377 27.032336408057372 26.991030021040007 21.797891578332244 39 24.364413511471156 23.883933196934763 27.47316811699516 24.27848517459892 40 25.58329729961222 25.215131676196606 25.002521209241024 24.19904981495015 41 23.582216978720993 24.903330621018736 26.890181651398958 24.624270748861314 42 23.916398083052087 25.2784036670125 24.757169559264995 26.04802869067042 43 22.026112820314452 24.55008503037194 26.945026586669503 26.47877556264411 44 23.68513757513549 23.526681301192635 26.488169657350397 26.300011466321482 45 25.0613724496069 23.410498453428087 27.951990650112794 23.57613844685222 46 22.331973492075115 24.04874429964952 30.07712828050767 23.5421539277677 47 24.142408949809287 24.888548736701484 26.79209625078917 24.176946062700054 48 23.5896769951054 23.612885934968 29.155401811678793 23.642035258247805 49 23.473494147340848 24.747637316107145 27.443742496812224 24.335126039739784 50 22.71961804716112 22.79380376565049 27.976857371394154 26.509720815794235 51 22.99025086377319 22.580088111082407 29.279182824279314 25.150478200865084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1411.0 1 1192.0 2 973.0 3 669.5 4 366.0 5 376.5 6 387.0 7 458.5 8 530.0 9 566.0 10 602.0 11 587.0 12 572.0 13 605.5 14 639.0 15 691.0 16 743.0 17 853.0 18 963.0 19 928.0 20 893.0 21 1182.5 22 1472.0 23 2397.0 24 3322.0 25 3604.5 26 4253.0 27 4619.0 28 5863.0 29 7107.0 30 8985.5 31 10864.0 32 13340.0 33 15816.0 34 16824.0 35 17832.0 36 18774.5 37 19717.0 38 21826.5 39 23936.0 40 25366.0 41 26796.0 42 28452.0 43 30108.0 44 38380.0 45 46652.0 46 52440.0 47 58228.0 48 61323.5 49 64419.0 50 62769.5 51 61120.0 52 59849.5 53 58579.0 54 53123.0 55 47667.0 56 43888.0 57 40109.0 58 39437.0 59 38765.0 60 36339.5 61 33914.0 62 31146.0 63 28378.0 64 24603.0 65 20828.0 66 18575.0 67 16322.0 68 13760.5 69 11199.0 70 9981.5 71 8764.0 72 7042.5 73 5321.0 74 4916.5 75 3510.0 76 2508.0 77 2003.0 78 1498.0 79 1160.5 80 823.0 81 570.0 82 317.0 83 258.0 84 199.0 85 149.5 86 100.0 87 76.5 88 53.0 89 33.0 90 13.0 91 10.0 92 7.0 93 5.5 94 4.0 95 3.0 96 2.0 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 723859.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.136198825740003 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6328253764983 18.25488911039637 2 10.047282967512936 4.850064387247265 3 3.5385252205882773 2.5621964482204245 4 1.6976039458985939 1.6389482546227696 5 1.0273252697015318 1.2397863484111573 6 0.662590687958692 0.9595452350792905 7 0.4468031273661335 0.7548890382650002 8 0.3925202296372827 0.7579157044520464 9 0.30770430268293725 0.6684131006181951 >10 4.137066078184276 25.207965576839285 >50 1.7820690130097214 30.31265240115958 >100 0.32076088418044835 10.667597386734819 >500 0.004038356455509241 0.6572301584026882 >1k 0.0028845403253637443 1.4679068495510887 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT 3362 0.46445509415507713 Illumina Single End Adapter 2 (95% over 21bp) AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC 2112 0.2917695297012263 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT 2019 0.2789217237058599 No Hit AATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT 1921 0.2653831754526779 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1128 0.155831453363155 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 914 0.1262676847286557 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5448574929647901 0.0 2 0.0 0.0 0.0 0.7643753824985252 0.0 3 0.0 0.0 0.0 1.0905438766389588 0.0 4 0.0 0.0 0.0 2.4633250398212914 0.0 5 0.0 0.0 0.0 2.6563184266549147 0.0 6 0.0 0.0 0.0 3.6425602223637474 0.0 7 0.0 0.0 0.0 4.352366966494856 0.0 8 0.0 0.0 0.0 4.984119835492824 0.0 9 0.0 0.0 0.0 6.0551847804613885 0.0 10 0.0 0.0 0.0 7.390942158624815 0.0 11 0.0 0.0 0.0 8.712608394728807 0.0 12 0.0 0.0 0.0 9.297252641743764 0.0 13 0.0 0.0 0.0 9.532519454755692 0.0 14 0.0 0.0 0.0 9.82304564839285 0.0 15 0.0 0.0 0.0 10.091053644425227 0.0 16 0.0 0.0 0.0 10.552469472645916 0.0 17 0.0 0.0 0.0 11.165710449134432 0.0 18 0.0 0.0 0.0 11.962136272395592 0.0 19 0.0 0.0 0.0 12.33251227103621 0.0 20 0.0 0.0 0.0 12.72181460754097 0.0 21 0.0 0.0 0.0 13.117195475914508 0.0 22 0.0 0.0 0.0 13.534680096538137 0.0 23 0.0 0.0 0.0 13.958657694385233 0.0 24 1.3814845156308065E-4 0.0 0.0 14.29850288523041 0.0 25 1.3814845156308065E-4 0.0 0.0 14.58999611802851 0.0 26 1.3814845156308065E-4 0.0 0.0 14.860905231543713 0.0 27 1.3814845156308065E-4 0.0 0.0 15.153365503502753 0.0 28 1.3814845156308065E-4 0.0 0.0 15.426761289146091 0.0 29 1.3814845156308065E-4 0.0 0.0 15.737871602066148 0.0 30 1.3814845156308065E-4 0.0 0.0 16.140159893017838 0.0 31 1.3814845156308065E-4 0.0 0.0 16.418114577562758 0.0 32 1.3814845156308065E-4 0.0 0.0 16.706430395974905 0.0 33 1.3814845156308065E-4 0.0 0.0 17.002482527674587 0.0 34 1.3814845156308065E-4 0.0 0.0 17.292594275957057 0.0 35 1.3814845156308065E-4 0.0 0.0 17.62760427099753 0.0 36 1.3814845156308065E-4 0.0 0.0 17.91025600289559 0.0 37 1.3814845156308065E-4 0.0 0.0 18.203130720209323 0.0 38 1.3814845156308065E-4 0.0 0.0 18.493933210749606 0.0 39 1.3814845156308065E-4 0.0 0.0 18.797445358833695 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATCG 30 2.1643827E-6 45.000004 27 ATTACGA 20 7.031553E-4 45.000004 37 CGCGACA 20 7.031553E-4 45.000004 23 GTCACGA 20 7.031553E-4 45.000004 45 ACGTCCC 20 7.031553E-4 45.000004 39 CATAACG 20 7.031553E-4 45.000004 1 CACCGTA 20 7.031553E-4 45.000004 25 ATACGAG 30 2.1643827E-6 45.000004 1 TATACGG 80 0.0 45.000004 2 CAATCGT 20 7.031553E-4 45.000004 25 CGATAGC 20 7.031553E-4 45.000004 16 GCGTATT 20 7.031553E-4 45.000004 11 TCGAAAC 20 7.031553E-4 45.000004 39 ATCGATG 30 2.1643827E-6 45.000004 1 TACGTAG 60 0.0 45.000004 1 ACGAACT 20 7.031553E-4 45.000004 38 GCGAATG 35 1.2111013E-7 45.0 1 ACGCATC 25 3.8893693E-5 45.0 37 ACGTTGC 25 3.8893693E-5 45.0 18 ACGTTAG 25 3.8893693E-5 45.0 1 >>END_MODULE