FastQCFastQC Report
Sat 18 Jun 2016
SRR3553220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070947
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT25090.23427863376992514TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT16400.15313549596758758No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGT13020.12157464374987743No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC12730.11886675997971889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG253.8906273E-545.01
ATTAGCG207.033072E-445.01
TCGATAG502.1827873E-1145.01
TCGTACG207.033072E-445.01
CGATACG253.8906273E-545.01
TCGAACG253.8906273E-545.01
TATCCGT253.8906273E-545.036
ACGAATC207.033072E-445.042
AACGTAG700.041.7857131
CGAAACG1250.041.41
TAACACG603.6379788E-1241.2499961
ACGTAGG1200.041.2499962
TCTAGCG501.0822987E-940.51
AAGTACG451.9281288E-840.01
CGAACCG451.9281288E-840.01
TAAGCGA451.9281288E-840.013
CATACGG2100.039.6428572
TTATACG800.039.3751
CGCAATG800.039.3751
TAACTCG403.4579352E-739.3751
Produced by FastQC (version 0.11.5)