##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070947
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926413725422453	33.0	31.0	34.0	30.0	34.0
2	32.13038180227406	34.0	31.0	34.0	30.0	34.0
3	32.29430588068317	34.0	31.0	34.0	30.0	34.0
4	35.92845117452124	37.0	35.0	37.0	35.0	37.0
5	35.70334666421401	37.0	35.0	37.0	33.0	37.0
6	35.04538413198786	37.0	35.0	37.0	33.0	37.0
7	35.495533392408774	37.0	35.0	37.0	35.0	37.0
8	35.90622411753336	37.0	35.0	37.0	35.0	37.0
9	37.59980932763246	39.0	37.0	39.0	35.0	39.0
10	37.11455748977307	39.0	37.0	39.0	33.0	39.0
11	37.085890338177336	39.0	37.0	39.0	33.0	39.0
12	36.98714782337501	39.0	37.0	39.0	33.0	39.0
13	36.96850731175306	39.0	37.0	39.0	33.0	39.0
14	38.21324864815906	40.0	38.0	41.0	33.0	41.0
15	38.21436635052902	40.0	37.0	41.0	33.0	41.0
16	38.129135241986766	40.0	37.0	41.0	33.0	41.0
17	38.13708521523474	40.0	37.0	41.0	33.0	41.0
18	38.125969819234754	40.0	37.0	41.0	33.0	41.0
19	38.191073881340536	40.0	37.0	41.0	34.0	41.0
20	38.08961601274386	40.0	37.0	41.0	33.0	41.0
21	38.082873382156166	40.0	37.0	41.0	33.0	41.0
22	38.061855535334615	40.0	37.0	41.0	33.0	41.0
23	37.97174650099398	40.0	37.0	41.0	33.0	41.0
24	37.826786012753196	40.0	37.0	41.0	33.0	41.0
25	37.87533743499912	40.0	37.0	41.0	33.0	41.0
26	37.832662120534444	40.0	37.0	41.0	33.0	41.0
27	37.738414692790585	40.0	37.0	41.0	33.0	41.0
28	37.719367998603104	40.0	37.0	41.0	33.0	41.0
29	37.538354372345225	40.0	36.0	41.0	32.0	41.0
30	37.575641931860304	40.0	36.0	41.0	33.0	41.0
31	37.55852530517383	40.0	36.0	41.0	33.0	41.0
32	37.52865361217689	40.0	36.0	41.0	33.0	41.0
33	37.322647152473465	40.0	36.0	41.0	32.0	41.0
34	37.30740643561259	39.0	36.0	41.0	32.0	41.0
35	37.26017907515498	39.0	36.0	41.0	32.0	41.0
36	37.22968737014997	39.0	36.0	41.0	32.0	41.0
37	37.1500597135059	39.0	35.0	41.0	31.0	41.0
38	37.02158930367236	39.0	35.0	41.0	31.0	41.0
39	37.03885159583061	39.0	35.0	41.0	31.0	41.0
40	36.98509730173389	39.0	35.0	41.0	31.0	41.0
41	36.95101625010388	39.0	35.0	41.0	31.0	41.0
42	36.879907222299515	39.0	35.0	41.0	31.0	41.0
43	36.8015980249256	39.0	35.0	41.0	31.0	41.0
44	36.69728193832188	39.0	35.0	40.0	31.0	41.0
45	36.49425788577773	39.0	35.0	40.0	31.0	41.0
46	36.369853970364545	38.0	35.0	40.0	30.0	41.0
47	36.25659346354208	38.0	35.0	40.0	30.0	41.0
48	36.15514586622867	38.0	35.0	40.0	30.0	41.0
49	36.061018892624936	38.0	35.0	40.0	30.0	41.0
50	35.97459818272986	38.0	35.0	40.0	30.0	41.0
51	34.773667604465956	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	7.0
11	11.0
12	17.0
13	18.0
14	27.0
15	44.0
16	86.0
17	140.0
18	254.0
19	505.0
20	819.0
21	1295.0
22	1945.0
23	2638.0
24	3456.0
25	4587.0
26	5789.0
27	7650.0
28	9696.0
29	12749.0
30	16883.0
31	22160.0
32	29498.0
33	40909.0
34	64083.0
35	78812.0
36	95638.0
37	143943.0
38	228198.0
39	299002.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.962381891914355	31.609687500875395	20.316224799173067	27.111705808037183
2	23.457276597254577	29.518921104405727	20.74892595058392	26.27487634775577
3	20.84865077356769	30.706001324061788	21.212254201188294	27.233093701182227
4	21.571282238990353	30.706094699364208	19.504886796452112	28.217736265193327
5	19.20235081661371	33.189784368414124	17.829360369840895	29.778504445131272
6	20.108651501895054	37.233868716192305	19.32233808022246	23.335141701690187
7	87.47267605212956	4.308429828927109	4.38910609021735	3.829788028725978
8	89.23625538892213	3.220981056952398	3.544713230439975	3.9980503236854856
9	82.09762014366724	7.183362015113727	6.4948125350740975	4.224205306144936
10	36.47257987556807	36.20543313534657	12.617804615914698	14.70418237317066
11	26.403360763884677	27.476429739286818	27.09097649090011	19.029233005928397
12	26.440150633037863	24.145919452596626	29.690358159647488	19.72357175471802
13	24.39784601852379	25.017577900680426	29.02468562870058	21.5598904520952
14	21.10067071479728	26.743620365900462	29.613603661058857	22.542105258243406
15	21.069016487277146	28.177584885153045	28.81141643797499	21.941982189594817
16	23.181072452698405	28.329693252794026	27.316104345032947	21.17312994947462
17	22.98246318445264	27.815101961161474	27.602206271645564	21.60022858274032
18	23.810328615701803	26.61354856963043	26.949886408944607	22.62623640572316
19	23.15044535350489	28.92888256841842	26.57311706368289	21.347555014393805
20	25.4784783934219	28.070016536766058	25.405832408139712	21.04567266167233
21	25.418251323361474	27.884199684951728	26.127063244026083	20.570485747660715
22	24.599630047051814	25.162776495942374	26.651552317715073	23.58604113929074
23	23.56699257759721	26.969588597755074	26.090740251384993	23.37267857326273
24	22.236954769937263	27.15026980793634	27.42040455783526	23.19237086429114
25	23.648509216609227	28.012217224568538	25.27576061186968	23.063512946952557
26	24.356013883039964	28.027157272955616	25.03989459795863	22.57693424604579
27	22.500273122759577	26.812904840295555	26.82859189110199	23.858230145842885
28	23.469228635964246	27.920522677592825	26.76575031257383	21.844498373869108
29	23.974015520842766	27.619200576685866	25.234021851688272	23.172762050783092
30	24.27804550551988	26.05021537013503	26.971175977896202	22.70056314644889
31	25.57409470309922	27.411160402895757	24.019115791911272	22.995629102093755
32	25.523018412675885	26.133226013985755	25.796421298159476	22.547334275178883
33	23.799030204109073	27.186125924065337	25.487909298966244	23.526934572859346
34	23.67530792840355	26.49328118011442	27.894564343520265	21.936846547961757
35	24.64594419705177	26.734282835658536	26.751837392513355	21.867935574776343
36	24.08345137527814	28.84988706257172	26.37226678817906	20.694394773971077
37	23.738616383443812	29.001621929003026	25.00945424936995	22.250307438183214
38	23.629554030218117	28.47909373666484	26.317922362171046	21.573429870945997
39	23.19573237517823	25.988774421143155	27.03037591963001	23.785117284048603
40	23.950765070540374	26.67022737819892	26.711032385356138	22.66797516590457
41	22.02601996177215	26.3797368123726	28.43446034210843	23.159782883746814
42	23.45989110572232	27.32450812225068	25.936857752998048	23.27874301902895
43	22.880403978908387	25.451866432232407	27.32226711499262	24.345462473866586
44	23.470349139593274	25.137471788986755	26.81327834150523	24.57890072991474
45	24.837550317616092	26.02799204815925	26.52026664251359	22.614190991711077
46	22.33714646943313	26.430719727493518	27.754688140496214	23.47744566257714
47	22.415861849372565	26.792175523158473	27.090136113178335	23.701826514290623
48	22.605507088586084	26.432960734751582	28.4028061145883	22.558726062074033
49	23.247088791508823	26.023976910155216	27.382680935657884	23.34625336267808
50	21.93871405401014	25.79474054271593	27.713883133338996	24.552662269934928
51	22.35722215945327	25.10086867043841	28.465927819023722	24.075981351084604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1018.0
1	883.0
2	748.0
3	549.0
4	350.0
5	371.0
6	392.0
7	423.5
8	455.0
9	504.5
10	554.0
11	632.0
12	710.0
13	899.0
14	1088.0
15	1204.5
16	1321.0
17	1458.0
18	1595.0
19	1834.0
20	2073.0
21	2670.5
22	3268.0
23	3999.0
24	4730.0
25	5851.5
26	8658.5
27	10344.0
28	13040.5
29	15737.0
30	18814.5
31	21892.0
32	24313.5
33	26735.0
34	28570.0
35	30405.0
36	35144.0
37	39883.0
38	43225.0
39	46567.0
40	46362.5
41	46158.0
42	50100.0
43	54042.0
44	62472.5
45	70903.0
46	76444.5
47	81986.0
48	86662.5
49	91339.0
50	87566.0
51	83793.0
52	80788.0
53	77783.0
54	71325.0
55	64867.0
56	60156.0
57	55445.0
58	53686.0
59	51927.0
60	49581.0
61	47235.0
62	41860.5
63	36486.0
64	33590.0
65	30694.0
66	25975.5
67	21257.0
68	17660.0
69	14063.0
70	11876.0
71	9689.0
72	7697.5
73	5706.0
74	4853.0
75	3481.0
76	2962.0
77	2464.0
78	1966.0
79	1388.0
80	810.0
81	650.5
82	491.0
83	420.5
84	350.0
85	201.5
86	53.0
87	55.0
88	57.0
89	40.0
90	23.0
91	16.0
92	9.0
93	8.5
94	8.0
95	5.0
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1070947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.43186664168865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52612256960276	18.9533841051228
2	8.975243407412027	4.565143868279957
3	3.131594656587912	2.3892689304650556
4	1.6423247855644894	1.6706953971526401
5	1.0107184584900046	1.2852228524305462
6	0.7572775190190196	1.1555388524664327
7	0.6292244741119819	1.1201647039311817
8	0.5416465585207679	1.102006643458382
9	0.4604213857511818	1.0538437753264986
>10	7.043544220666941	43.06754220172729
>50	1.2087494385860826	19.76001951918885
>100	0.06938213975417677	2.8537491016353087
>500	0.002250231559594902	0.3820895354662761
>1k	0.001500154373063268	0.641330513348787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	2509	0.23427863376992514	TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	1640	0.15313549596758758	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGT	1302	0.12157464374987743	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	1273	0.11886675997971889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26387860463683077	0.0
2	0.0	0.0	0.0	0.42121598921328507	0.0
3	0.0	0.0	0.0	0.6350454317533921	0.0
4	0.0	0.0	0.0	1.4893360735872083	0.0
5	0.0	0.0	0.0	1.6413510659257649	0.0
6	0.0	0.0	0.0	2.4182335820540137	0.0
7	0.0	0.0	0.0	2.9933320696542407	0.0
8	0.0	0.0	0.0	3.524264039210157	0.0
9	0.0	0.0	0.0	4.2655705651166675	0.0
10	0.0	0.0	0.0	5.276918465619681	0.0
11	0.0	0.0	0.0	6.243632971566287	0.0
12	0.0	0.0	0.0	6.683243895356166	0.0
13	0.0	0.0	0.0	6.879145279831775	0.0
14	0.0	0.0	0.0	7.102592378521066	0.0
15	0.0	0.0	0.0	7.291210489407972	0.0
16	0.0	0.0	0.0	7.642301626504393	0.0
17	0.0	0.0	0.0	8.066038748882997	0.0
18	0.0	0.0	0.0	8.578015532047804	0.0
19	0.0	0.0	0.0	8.85375280009188	0.0
20	0.0	0.0	0.0	9.150219385273035	0.0
21	0.0	0.0	0.0	9.488704856542855	0.0
22	0.0	0.0	0.0	9.844558134062657	0.0
23	0.0	0.0	0.0	10.211242946663093	0.0
24	0.0	0.0	0.0	10.495477367227323	0.0
25	0.0	0.0	0.0	10.751512446460936	0.0
26	0.0	0.0	0.0	10.985417579021185	0.0
27	0.0	0.0	0.0	11.241452658254797	0.0
28	0.0	0.0	0.0	11.491885219343255	0.0
29	0.0	0.0	0.0	11.764354351802657	0.0
30	0.0	0.0	0.0	12.137201934362764	0.0
31	0.0	0.0	0.0	12.40686980774959	0.0
32	0.0	0.0	0.0	12.701468886882358	0.0
33	0.0	0.0	0.0	12.996254716619964	0.0
34	9.337530241926071E-5	0.0	0.0	13.27582037206323	0.0
35	9.337530241926071E-5	0.0	0.0	13.569018821659709	0.0
36	9.337530241926071E-5	0.0	0.0	13.8428885836554	0.0
37	9.337530241926071E-5	0.0	0.0	14.121707236679313	0.0
38	9.337530241926071E-5	0.0	0.0	14.421068456235462	0.0
39	9.337530241926071E-5	0.0	0.0	14.724071312585963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	25	3.8906273E-5	45.0	1
ATTAGCG	20	7.033072E-4	45.0	1
TCGATAG	50	2.1827873E-11	45.0	1
TCGTACG	20	7.033072E-4	45.0	1
CGATACG	25	3.8906273E-5	45.0	1
TCGAACG	25	3.8906273E-5	45.0	1
TATCCGT	25	3.8906273E-5	45.0	36
ACGAATC	20	7.033072E-4	45.0	42
AACGTAG	70	0.0	41.785713	1
CGAAACG	125	0.0	41.4	1
TAACACG	60	3.6379788E-12	41.249996	1
ACGTAGG	120	0.0	41.249996	2
TCTAGCG	50	1.0822987E-9	40.5	1
AAGTACG	45	1.9281288E-8	40.0	1
CGAACCG	45	1.9281288E-8	40.0	1
TAAGCGA	45	1.9281288E-8	40.0	13
CATACGG	210	0.0	39.642857	2
TTATACG	80	0.0	39.375	1
CGCAATG	80	0.0	39.375	1
TAACTCG	40	3.4579352E-7	39.375	1
>>END_MODULE