SRR3553215_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028510
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	5680	0.5522552041302466	TruSeq Adapter, Index 27 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1673	0.16266249234329272	TruSeq Adapter, Index 27 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGT	1388	0.13495250410788423	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1352	0.1314522950676221	Illumina Single End Adapter 1 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1258	0.12231286035138209	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	1235	0.12007661568677018	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	40	6.8139343E-9	45.000004	1
GCGAAGT	20	7.0329395E-4	45.000004	18
CTATACG	35	1.2117562E-7	45.000004	1
TAAACCG	20	7.0329395E-4	45.000004	1
CATAGCG	30	2.1652777E-6	45.000004	1
CCCGGTC	20	7.0329395E-4	45.000004	11
CCGCAAT	20	7.0329395E-4	45.000004	43
TACGTAT	20	7.0329395E-4	45.000004	16
TAATCCG	20	7.0329395E-4	45.000004	1
ATTGTCG	25	3.890518E-5	45.0	1
ACGATTG	25	3.890518E-5	45.0	37
CTACGCG	50	2.1827873E-11	45.0	1
CGAATAT	90	0.0	45.0	13
TCGTACT	25	3.890518E-5	45.0	29
TGCGTAT	25	3.890518E-5	45.0	21
ACGGGTA	190	0.0	42.63158	5
CAGTACG	65	0.0	41.53846	1
CTTAACG	60	3.6379788E-12	41.250004	1
GCCGATG	210	0.0	40.714287	9
AGTAACG	50	1.0804797E-9	40.5	1