FastQCFastQC Report
Sat 18 Jun 2016
SRR3553214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154219
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT32300.2798429067620616TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG14050.1217273325079556No Hit
AATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT13780.11938808839570307No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC12470.10803842251773711No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGT11740.10171379954757286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG850.045.0000041
TTACGAG351.211938E-745.0000041
GTCGAAC207.0332986E-445.021
AACGGTT207.0332986E-445.014
TCGCGCG207.0332986E-445.01
ACGGTTA207.0332986E-445.020
CAATACG302.1655105E-644.9999961
TTTAGCG700.041.7857171
ATTAGCG556.184564E-1140.9090921
TATAGCG556.184564E-1140.9090921
TCTTACG501.0822987E-940.51
TTCGATC501.0822987E-940.513
GCGATAG950.040.2631571
CGGGTAC850.039.7058836
ATAACGG2500.039.62
TAACGGG6950.038.8489233
GTATTCG356.2483105E-638.571438
TCGTCGG356.2483105E-638.571432
TAGTAGG2050.038.4146352
TTACGGG5300.038.2075463
Produced by FastQC (version 0.11.5)