##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1154219
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90996769243965	33.0	31.0	34.0	30.0	34.0
2	32.12233813513727	34.0	31.0	34.0	30.0	34.0
3	32.27800096862034	34.0	31.0	34.0	30.0	34.0
4	35.92100459271594	37.0	35.0	37.0	35.0	37.0
5	35.69424260040772	37.0	35.0	37.0	33.0	37.0
6	35.0124673047316	37.0	35.0	37.0	33.0	37.0
7	35.4620665575597	37.0	35.0	37.0	35.0	37.0
8	35.863998946473764	37.0	35.0	37.0	35.0	37.0
9	37.56529046913974	39.0	37.0	39.0	35.0	39.0
10	37.09591680608273	39.0	37.0	39.0	33.0	39.0
11	37.075143451979216	39.0	37.0	39.0	33.0	39.0
12	36.95379299768935	39.0	37.0	39.0	33.0	39.0
13	36.934649316984036	39.0	37.0	39.0	33.0	39.0
14	38.15367794153449	40.0	37.0	41.0	33.0	41.0
15	38.16373062651022	40.0	37.0	41.0	33.0	41.0
16	38.087031144003	40.0	37.0	41.0	33.0	41.0
17	38.09363474349322	40.0	37.0	41.0	33.0	41.0
18	38.082658490286505	40.0	37.0	41.0	33.0	41.0
19	38.135461294607005	40.0	37.0	41.0	34.0	41.0
20	38.03670533928137	40.0	37.0	41.0	33.0	41.0
21	38.04965175586262	40.0	37.0	41.0	33.0	41.0
22	38.00103446572964	40.0	37.0	41.0	33.0	41.0
23	37.92949604884342	40.0	37.0	41.0	33.0	41.0
24	37.77584583168359	40.0	37.0	41.0	33.0	41.0
25	37.81590668668598	40.0	37.0	41.0	33.0	41.0
26	37.781025091425455	40.0	37.0	41.0	33.0	41.0
27	37.69748808501679	40.0	37.0	41.0	33.0	41.0
28	37.67738791338559	40.0	36.0	41.0	33.0	41.0
29	37.48865943118247	40.0	36.0	41.0	32.0	41.0
30	37.55411408060342	40.0	36.0	41.0	33.0	41.0
31	37.53014809148004	40.0	36.0	41.0	33.0	41.0
32	37.50305704550003	40.0	36.0	41.0	33.0	41.0
33	37.31585686945025	40.0	36.0	41.0	32.0	41.0
34	37.29050206243356	39.0	36.0	41.0	32.0	41.0
35	37.27989662273797	39.0	36.0	41.0	32.0	41.0
36	37.21755576714644	39.0	35.0	41.0	32.0	41.0
37	37.14759850600276	39.0	35.0	41.0	32.0	41.0
38	36.99299093153032	39.0	35.0	41.0	31.0	41.0
39	36.98948553090878	39.0	35.0	41.0	31.0	41.0
40	36.88918827362918	39.0	35.0	41.0	31.0	41.0
41	36.86876667252922	39.0	35.0	41.0	31.0	41.0
42	36.819770771404734	39.0	35.0	41.0	31.0	41.0
43	36.75699412329896	39.0	35.0	41.0	31.0	41.0
44	36.66327880584187	39.0	35.0	40.0	31.0	41.0
45	36.4756904885468	39.0	35.0	40.0	31.0	41.0
46	36.32616167295808	38.0	35.0	40.0	30.0	41.0
47	36.20830275710242	38.0	35.0	40.0	30.0	41.0
48	36.12872687072384	38.0	35.0	40.0	30.0	41.0
49	36.01661556429066	38.0	35.0	40.0	30.0	41.0
50	35.93418060177488	38.0	35.0	40.0	30.0	41.0
51	34.77635873261487	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	4.0
10	9.0
11	11.0
12	14.0
13	20.0
14	30.0
15	56.0
16	111.0
17	190.0
18	320.0
19	565.0
20	940.0
21	1487.0
22	2125.0
23	2932.0
24	3885.0
25	4904.0
26	6334.0
27	8073.0
28	10568.0
29	13987.0
30	18011.0
31	24433.0
32	32170.0
33	44550.0
34	70859.0
35	85601.0
36	105009.0
37	156361.0
38	245898.0
39	314678.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.611426427740316	31.884243804685248	21.045659445911046	26.45867032166339
2	24.569774020354888	29.50410623980371	20.04186380574224	25.88425593409916
3	21.274125620874372	29.661701982032874	21.1806424950551	27.883529902037658
4	21.377658832509255	30.515006250980097	19.446656137180206	28.66067877933044
5	18.85517393146361	32.9713858461869	17.22411431452783	30.949325907821652
6	20.122784324292013	37.455716809375	19.570376159117117	22.851122707215875
7	86.71595251854284	4.462931211494526	4.612816112020336	4.208300157942297
8	88.21852698664638	3.8984802710750737	3.7665295754098658	4.116463166868679
9	81.21439692120819	6.940537281053249	6.945302407948578	4.89976338978998
10	37.60360902047185	32.75678185855544	13.54578290601697	16.09382621495574
11	28.235282905583777	27.890201079691117	24.00480324791049	19.86971276681462
12	27.42928335090654	23.463138277917793	28.48445572287408	20.623122648301578
13	26.42323510529631	23.73405740158497	27.581854050227903	22.26085344289082
14	22.93126347772823	26.150323292200177	27.777830723632167	23.14058250643942
15	21.457972880363258	28.392272177117167	27.523806140775708	22.625948801743863
16	23.484451390940542	26.47313897969103	26.979022178633343	23.063387450735085
17	23.282496649249406	26.931110993667577	26.83156316089061	22.954829196192403
18	24.04985535673906	25.879664084545478	26.219720867530338	23.85075969118512
19	22.94503902638927	29.49059060715514	25.532936123907163	22.031434242548425
20	25.7162635513711	26.648582288109967	25.75663717197516	21.87851698854377
21	26.62094455211706	26.203692713427866	25.18837413003945	21.98698860441563
22	24.47793702928127	24.509386866790443	25.74147540458093	25.27120069934735
23	23.952126936049396	26.526681678260367	25.015270065732757	24.505921319957476
24	22.644316199958585	27.253666765145958	26.45303880805982	23.648978226835634
25	24.069349057674497	27.198997763855903	24.090922086709714	24.640731091759882
26	25.27120069934735	27.02485403549933	24.124971084343613	23.57897418080971
27	22.54008987895711	25.90063064288493	26.05302806486464	25.506251413293317
28	22.83336177969692	26.780619622446	27.050499082063283	23.335519515793795
29	23.972487023693077	27.150480108194376	25.48173266945008	23.395300198662472
30	24.483568542884843	25.682387831078852	26.259401378767805	23.5746422472685
31	24.550107042077805	26.368652742677085	24.3812482726415	24.699991942603614
32	24.615865793233347	26.165398420923587	25.632137402000833	23.586598383842233
33	24.06172485464197	25.64703925338259	25.7058669108722	24.58536898110324
34	22.64189031717551	25.943516784942894	27.065227656103392	24.349365241778205
35	24.031314681182685	24.653813531054332	26.994183946027572	24.32068784173541
36	23.95091399465786	27.679582470917563	26.52971403173921	21.839789502685367
37	23.654003269743438	27.567818585554388	25.294939695153175	23.483238449549003
38	24.363573983793373	26.28019465976561	27.009518990763453	22.346712365677572
39	24.60642217811351	25.13708403691154	25.450282831940907	24.806210953034043
40	24.210396813776242	25.712364811184013	25.968555360811074	24.108683014228667
41	21.93587178862937	24.701031606653505	27.879977716533865	25.483118888183263
42	22.915495239638233	25.426977029489205	26.21859456480962	25.438933166062938
43	22.43092515371866	24.02135123403791	27.811273250570302	25.73645036167313
44	23.684413443202722	23.722794374377827	26.788157186807705	25.804634995611753
45	24.72156497163883	24.046303171235266	27.25713231197892	23.97499954514698
46	22.189289900790058	24.591953520085877	28.441656219486944	24.77710035963712
47	22.172828553333463	25.92003770514954	27.666326754281467	24.240806987235526
48	22.818719844327635	24.841992724084424	28.610514988923246	23.728772442664695
49	22.265791847127797	25.312873900013777	27.789353666851785	24.63198058600664
50	22.05439349031683	24.000298037027633	28.185898863214	25.759409609441537
51	22.86264565043549	23.587464770550476	28.70226534132604	24.847624237687995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1096.0
1	905.0
2	714.0
3	514.5
4	315.0
5	306.0
6	297.0
7	356.5
8	416.0
9	444.5
10	473.0
11	566.0
12	659.0
13	831.5
14	1004.0
15	968.5
16	933.0
17	1043.0
18	1153.0
19	1399.0
20	1645.0
21	2070.0
22	2495.0
23	3737.0
24	4979.0
25	5465.0
26	6787.5
27	7624.0
28	11115.0
29	14606.0
30	15496.0
31	16386.0
32	21155.0
33	25924.0
34	28303.5
35	30683.0
36	32779.0
37	34875.0
38	38591.5
39	42308.0
40	45182.5
41	48057.0
42	50786.5
43	53516.0
44	62860.5
45	72205.0
46	77554.0
47	82903.0
48	91020.5
49	99138.0
50	97271.0
51	95404.0
52	90900.5
53	86397.0
54	79597.5
55	72798.0
56	68931.5
57	65065.0
58	63400.5
59	61736.0
60	59792.5
61	57849.0
62	53850.5
63	49852.0
64	43240.0
65	36628.0
66	31993.0
67	27358.0
68	22366.5
69	17375.0
70	15208.5
71	13042.0
72	10292.0
73	7542.0
74	6500.5
75	4457.0
76	3455.0
77	2474.5
78	1494.0
79	1337.5
80	1181.0
81	925.5
82	670.0
83	494.5
84	319.0
85	199.0
86	79.0
87	77.0
88	75.0
89	65.0
90	55.0
91	37.5
92	20.0
93	14.5
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1154219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.880802337938636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66359019652589	17.08362849127562
2	9.838700711613336	4.502347324891219
3	3.3588598494573767	2.3056022488881767
4	1.5527990633585342	1.421171537569715
5	0.9720748811914601	1.1120926607108492
6	0.6509277081438419	0.8936248935795995
7	0.5037817791425108	0.8068851917010393
8	0.4031888711323044	0.7380227892187896
9	0.3628242467681033	0.74715388863412
>10	5.550136765657003	32.03673690211758
>50	1.914589198810292	30.124434733708938
>100	0.2227167266349639	6.909204863722287
>500	0.003873334376260238	0.571631238725099
>1k	0.001936667188130119	0.7474632352569744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	3230	0.2798429067620616	TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1405	0.1217273325079556	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	1378	0.11938808839570307	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	1247	0.10803842251773711	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGT	1174	0.10171379954757286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.315191484458322	0.0
2	0.0	0.0	0.0	0.4734803360540764	0.0
3	0.0	0.0	0.0	0.6947555013390007	0.0
4	0.0	0.0	0.0	1.562961621667985	0.0
5	0.0	0.0	0.0	1.6934394599291815	0.0
6	0.0	0.0	0.0	2.4538670737529014	0.0
7	0.0	0.0	0.0	2.99371263165829	0.0
8	0.0	0.0	0.0	3.4672796063831908	0.0
9	0.0	0.0	0.0	4.126599891355107	0.0
10	0.0	0.0	0.0	5.107956115780454	0.0
11	0.0	0.0	0.0	6.0114241751348745	0.0
12	0.0	0.0	0.0	6.439505847677087	0.0
13	0.0	0.0	0.0	6.607411591734325	0.0
14	0.0	0.0	0.0	6.815431040383151	0.0
15	0.0	0.0	0.0	6.993213592914343	0.0
16	0.0	0.0	0.0	7.344966596460464	0.0
17	0.0	0.0	0.0	7.765250788628501	0.0
18	0.0	0.0	0.0	8.291147520531199	0.0
19	0.0	0.0	0.0	8.544738910033537	0.0
20	0.0	0.0	0.0	8.837577617419225	0.0
21	0.0	0.0	0.0	9.17633482034172	0.0
22	8.663867082416768E-5	0.0	0.0	9.52886757192526	0.0
23	8.663867082416768E-5	0.0	0.0	9.88417276097517	0.0
24	8.663867082416768E-5	0.0	0.0	10.172073064123879	0.0
25	8.663867082416768E-5	0.0	0.0	10.427223949701053	0.0
26	8.663867082416768E-5	0.0	0.0	10.654650460614494	0.0
27	8.663867082416768E-5	0.0	0.0	10.89221369601436	0.0
28	8.663867082416768E-5	0.0	0.0	11.151523237791094	0.0
29	8.663867082416768E-5	0.0	0.0	11.417763873233762	0.0
30	8.663867082416768E-5	0.0	0.0	11.767870742034225	0.0
31	8.663867082416768E-5	0.0	0.0	12.025274233052826	0.0
32	8.663867082416768E-5	0.0	0.0	12.296973104757416	0.0
33	8.663867082416768E-5	0.0	0.0	12.559661554696293	0.0
34	8.663867082416768E-5	0.0	0.0	12.820963785901982	0.0
35	8.663867082416768E-5	0.0	0.0	13.111376610504593	0.0
36	8.663867082416768E-5	0.0	0.0	13.374584892468414	0.0
37	8.663867082416768E-5	0.0	0.0	13.648882924297729	0.0
38	8.663867082416768E-5	0.0	0.0	13.933664235296767	0.0
39	8.663867082416768E-5	0.0	0.0	14.213680419400477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	85	0.0	45.000004	1
TTACGAG	35	1.211938E-7	45.000004	1
GTCGAAC	20	7.0332986E-4	45.0	21
AACGGTT	20	7.0332986E-4	45.0	14
TCGCGCG	20	7.0332986E-4	45.0	1
ACGGTTA	20	7.0332986E-4	45.0	20
CAATACG	30	2.1655105E-6	44.999996	1
TTTAGCG	70	0.0	41.785717	1
ATTAGCG	55	6.184564E-11	40.909092	1
TATAGCG	55	6.184564E-11	40.909092	1
TCTTACG	50	1.0822987E-9	40.5	1
TTCGATC	50	1.0822987E-9	40.5	13
GCGATAG	95	0.0	40.263157	1
CGGGTAC	85	0.0	39.705883	6
ATAACGG	250	0.0	39.6	2
TAACGGG	695	0.0	38.848923	3
GTATTCG	35	6.2483105E-6	38.57143	8
TCGTCGG	35	6.2483105E-6	38.57143	2
TAGTAGG	205	0.0	38.414635	2
TTACGGG	530	0.0	38.207546	3
>>END_MODULE