FastQCFastQC Report
Sat 18 Jun 2016
SRR3553211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415883
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC40430.9721484167422088No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGT39510.9500268104250473No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT35090.8437469192056419No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT34970.8408614922947079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14050.33783540082186575No Hit
AACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG9690.23299822305792736No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT9590.23059370063214896No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT9260.2226587766270802No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT6570.15797712337364114No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC5820.13994320518030312No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTAT5810.13970275293772527No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT5790.1392218484525696No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTGACTTC5590.1344128036010128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT207.028087E-445.00000431
TATCATG207.028087E-445.00000431
TACCCCA207.028087E-445.00000441
ATCTACG207.028087E-445.0000041
CACGGTT207.028087E-445.00000432
ACGTTTG207.028087E-445.0000041
CTATCTA207.028087E-445.00000421
AGATACG207.028087E-445.0000041
GAATGCG207.028087E-445.00000410
CGACGAA207.028087E-445.0000041
CCCTATC207.028087E-445.00000444
TTACTCG207.028087E-445.0000041
TAACGTT207.028087E-445.00000423
TGTGCGC302.1621472E-645.00000442
CCGTCAA207.028087E-445.00000413
CGTTAGA207.028087E-445.00000444
AAACGTA207.028087E-445.00000431
TGCGCGT302.1621472E-645.00000444
CATAGCG207.028087E-445.0000041
AGCATCG207.028087E-445.0000041