##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415883
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745577482128386	31.0	31.0	34.0	30.0	34.0
2	31.93387082424624	33.0	31.0	34.0	30.0	34.0
3	32.06770654246507	33.0	31.0	34.0	30.0	34.0
4	35.74433915307911	37.0	35.0	37.0	33.0	37.0
5	35.518073592813366	37.0	35.0	37.0	33.0	37.0
6	35.54079152069212	37.0	35.0	37.0	33.0	37.0
7	35.58498904740035	37.0	35.0	37.0	33.0	37.0
8	35.68114109016238	37.0	35.0	37.0	35.0	37.0
9	37.397897966495385	39.0	37.0	39.0	35.0	39.0
10	36.93579684670929	39.0	37.0	39.0	33.0	39.0
11	36.92209106888235	39.0	37.0	39.0	33.0	39.0
12	36.770387344517566	39.0	35.0	39.0	33.0	39.0
13	36.749643529550376	39.0	35.0	39.0	33.0	39.0
14	37.85727476237307	40.0	37.0	41.0	33.0	41.0
15	37.88746594595114	40.0	37.0	41.0	33.0	41.0
16	37.77881038657507	40.0	36.0	41.0	33.0	41.0
17	37.76440008367738	39.0	36.0	41.0	33.0	41.0
18	37.6486079017416	39.0	36.0	41.0	33.0	41.0
19	37.684355936645645	39.0	36.0	41.0	32.0	41.0
20	37.667517066097915	39.0	36.0	41.0	33.0	41.0
21	37.62773183804099	39.0	36.0	41.0	33.0	41.0
22	37.5839959796385	39.0	36.0	41.0	32.0	41.0
23	37.48960645181457	39.0	36.0	41.0	32.0	41.0
24	37.37212148609104	39.0	36.0	41.0	32.0	41.0
25	37.399864865839675	39.0	36.0	41.0	32.0	41.0
26	37.33897514445169	39.0	36.0	41.0	32.0	41.0
27	37.311327945600084	39.0	36.0	41.0	32.0	41.0
28	37.25872420849133	39.0	36.0	41.0	32.0	41.0
29	37.14364136067115	39.0	35.0	41.0	32.0	41.0
30	37.128468824164486	39.0	35.0	41.0	31.0	41.0
31	36.94808395630502	39.0	35.0	41.0	31.0	41.0
32	36.84915228561879	39.0	35.0	41.0	31.0	41.0
33	36.7196759665579	39.0	35.0	41.0	31.0	41.0
34	36.715619537225614	39.0	35.0	41.0	31.0	41.0
35	36.61708220821721	39.0	35.0	41.0	30.0	41.0
36	36.60297487514517	39.0	35.0	40.0	30.0	41.0
37	36.53608827482729	39.0	35.0	40.0	30.0	41.0
38	36.423811504678	39.0	35.0	40.0	30.0	41.0
39	36.32864050706569	39.0	35.0	40.0	30.0	41.0
40	36.13961378560797	38.0	35.0	40.0	30.0	41.0
41	36.15060726213863	38.0	35.0	40.0	30.0	41.0
42	36.095262369464486	38.0	35.0	40.0	30.0	41.0
43	36.045943209989346	38.0	35.0	40.0	30.0	41.0
44	35.97341319553817	38.0	35.0	40.0	30.0	41.0
45	35.72708910919658	38.0	35.0	40.0	29.0	41.0
46	35.591769800641046	38.0	34.0	40.0	29.0	41.0
47	35.443396339835964	38.0	34.0	40.0	28.0	41.0
48	35.410427932856116	38.0	34.0	40.0	28.0	41.0
49	35.24457359401563	37.0	34.0	40.0	27.0	41.0
50	35.20386262482477	37.0	34.0	40.0	27.0	41.0
51	33.842640838889785	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	1.0
12	4.0
13	4.0
14	9.0
15	19.0
16	39.0
17	84.0
18	168.0
19	311.0
20	567.0
21	987.0
22	1390.0
23	1849.0
24	2355.0
25	2831.0
26	3033.0
27	3874.0
28	4577.0
29	5883.0
30	7715.0
31	10296.0
32	13302.0
33	18003.0
34	28587.0
35	34818.0
36	38992.0
37	55441.0
38	84348.0
39	96369.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.234736692771765	34.577753839421185	19.737281879759454	26.450227588047596
2	22.739809032828944	34.42530711762683	19.25661784684635	23.578266002697873
3	21.229528497197528	30.227010962217737	23.641745394738425	24.901715145846307
4	22.834547216404612	31.310007862788332	18.44028248329458	27.415162437512475
5	17.2019534340187	35.794682639107634	18.68891010212007	28.3144538247536
6	21.038368964348145	33.99609986462539	22.785494958918733	22.18003621210773
7	80.21198269705663	8.050581533748675	6.838942683398937	4.8984930857957645
8	81.83239035978869	4.419271766338129	5.198337032290332	8.550000841582849
9	76.97357189401826	7.745688090159973	9.631314576455399	5.64942543936636
10	36.46987253626621	29.622994928862205	15.379806339763826	18.52732619510776
11	26.134994698028052	28.622713599738386	24.51170160838505	20.730590093848512
12	26.85226373763775	23.403457222343786	31.675976175991806	18.068302864026663
13	27.248288581163454	25.066905836497284	26.169138916474104	21.51566666586516
14	21.427661145081668	27.218232050841223	30.401579290329252	20.952527513747857
15	18.804086726314853	28.846093733093202	28.769870372196028	23.57994916839592
16	21.467576217349592	27.535388558801394	28.132191024879592	22.864844198969422
17	20.41223132467545	26.325192421907122	28.798003284577632	24.464572968839793
18	22.746782147863705	26.119124850017915	26.21097760668265	24.923115395435737
19	22.82180324754799	29.916587117049747	26.61349466075795	20.64811497464431
20	24.17530892101865	28.669842239283643	25.297259084886853	21.85758975481085
21	23.39528184609614	26.69284390080864	26.4990393932909	23.41283485980432
22	21.449301846913677	26.903720517549406	25.96283089234232	25.684146743194603
23	23.335649689936833	27.38438455046251	23.918505925945517	25.361459833655136
24	19.279701262133823	29.665074071313324	25.946480139847022	25.108744526705827
25	22.08770255095784	26.1768333882366	25.736324879833994	25.99913918097157
26	23.772791866943347	26.71544641161096	24.28784057054508	25.223921150900612
27	19.516546721072995	25.738008045532034	26.78181123056244	27.963634002832528
28	20.83061822676089	26.265560265747816	28.56692867946033	24.336892828030958
29	21.523120685385074	27.449547108201106	24.434516438517566	26.592815767896262
30	22.611888439777534	24.848334747994027	25.954415063852093	26.585361748376346
31	23.89614386738578	26.425701459304662	24.353964937253988	25.324189736055576
32	24.65308752702082	24.753115659933204	23.96371094755015	26.630085865495822
33	22.39403870800201	26.414159751660925	26.119846206745645	25.07195533359142
34	20.547365485004196	24.777401336433563	26.548572555261934	28.126660623300303
35	20.879189579761615	24.612691550267744	29.31401379714968	25.19410507282096
36	22.31637263364937	25.462209323295255	26.906605944460342	25.314812098595034
37	21.747703079952778	26.677454957283658	24.901955598088886	26.67288636467468
38	23.070430866373474	26.36582885090278	28.347395781986762	22.216344500736987
39	22.51739070844444	24.38305965860591	27.488740823741292	25.610808809208358
40	23.138238398780427	25.813990954186632	25.98495249865948	25.062818148373463
41	20.228766263588557	22.433472875784776	31.256627464936056	26.081133395690614
42	23.12212809852771	24.18011796587021	26.96912352753058	25.728630408071503
43	20.51562578898392	22.21345907382605	29.00791809234809	28.262997044841935
44	22.240630177237346	20.84095767319174	27.9780611373872	28.94035101218371
45	25.252534967767374	23.263033112678325	28.491667127533464	22.992764792020832
46	20.852739833078054	23.04277885847702	30.84689684358341	25.25758446486151
47	21.628679219876744	25.57858820870293	26.17515022253855	26.617582348881776
48	20.579586085509625	22.90187384432641	30.758650870557346	25.759889199606622
49	21.891493521014326	22.7826095320078	29.50012383290493	25.82577311407295
50	21.563757114380728	21.82176237066675	27.065304424561713	29.549176090390805
51	21.757561621898468	20.788779536552347	31.366995044279278	26.086663797269903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1629.0
1	1301.0
2	973.0
3	746.5
4	520.0
5	439.5
6	359.0
7	369.5
8	380.0
9	406.0
10	432.0
11	490.0
12	548.0
13	574.0
14	600.0
15	688.0
16	776.0
17	780.0
18	784.0
19	953.5
20	1123.0
21	1042.5
22	962.0
23	1204.0
24	1446.0
25	1635.5
26	2177.0
27	2529.0
28	3119.5
29	3710.0
30	4825.5
31	5941.0
32	7381.5
33	8822.0
34	8967.5
35	9113.0
36	10246.5
37	11380.0
38	11236.5
39	11093.0
40	12127.0
41	13161.0
42	15658.0
43	18155.0
44	26735.0
45	35315.0
46	38323.0
47	41331.0
48	41780.5
49	42230.0
50	38838.5
51	35447.0
52	32131.0
53	28815.0
54	26931.5
55	25048.0
56	23651.0
57	22254.0
58	21031.5
59	19809.0
60	18866.0
61	17923.0
62	16659.0
63	15395.0
64	13566.0
65	11737.0
66	9943.0
67	8149.0
68	6679.5
69	5210.0
70	4787.0
71	4364.0
72	3474.5
73	2585.0
74	2227.5
75	1370.0
76	870.0
77	737.5
78	605.0
79	465.0
80	325.0
81	236.0
82	147.0
83	121.5
84	96.0
85	72.5
86	49.0
87	38.5
88	28.0
89	20.0
90	12.0
91	7.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.92918074499053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32678993950312	18.529059805968167
2	11.01275146115982	5.490777433498234
3	3.9612227709721797	2.96250115286213
4	1.9142227811932297	1.9088002279417795
5	1.138339953230181	1.4188941221659623
6	0.7412292600313902	1.1086942918078868
7	0.5037927094986087	0.8791397659169525
8	0.39405526190283546	0.7858779877992291
9	0.3312058908029841	0.7431022364069868
>10	3.8457791464055155	23.601204963479965
>50	1.49242168645724	26.342326995070938
>100	0.3252202338135429	10.704352622890838
>500	0.007980864633461177	1.4454183691334406
>1k	0.004988040395913235	4.079850025057484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	4043	0.9721484167422088	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGT	3951	0.9500268104250473	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	3509	0.8437469192056419	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	3497	0.8408614922947079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1405	0.33783540082186575	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	969	0.23299822305792736	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	959	0.23059370063214896	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	926	0.2226587766270802	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT	657	0.15797712337364114	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	582	0.13994320518030312	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTAT	581	0.13970275293772527	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	579	0.1392218484525696	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTGACTTC	559	0.1344128036010128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.958202186672694	0.0
2	0.0	0.0	0.0	1.2751182423902876	0.0
3	0.0	0.0	0.0	1.9111144240086755	0.0
4	0.0	0.0	0.0	4.407970510936971	0.0
5	0.0	0.0	0.0	4.708776266401848	0.0
6	0.0	0.0	0.0	6.2669067983062545	0.0
7	0.0	0.0	0.0	7.398715504120149	0.0
8	0.0	0.0	0.0	8.203749612270759	0.0
9	0.0	0.0	0.0	10.18772106578052	0.0
10	0.0	0.0	0.0	11.821834506339524	0.0
11	0.0	0.0	0.0	14.209284822894901	0.0
12	0.0	0.0	0.0	15.012635765347465	0.0
13	0.0	0.0	0.0	15.46636914709185	0.0
14	0.0	0.0	0.0	15.917938458653035	0.0
15	0.0	0.0	0.0	16.2199464753308	0.0
16	2.404522425778404E-4	0.0	0.0	16.804966781522687	0.0
17	2.404522425778404E-4	0.0	0.0	17.51502225385505	0.0
18	2.404522425778404E-4	0.0	0.0	18.65717040609979	0.0
19	2.404522425778404E-4	0.0	0.0	19.039008567313402	0.0
20	2.404522425778404E-4	0.0	0.0	19.491058783359744	0.0
21	2.404522425778404E-4	0.0	0.0	19.965951962450976	0.0
22	2.404522425778404E-4	0.0	0.0	20.425937102502388	0.0
23	2.404522425778404E-4	0.0	0.0	20.90852475335611	0.0
24	2.404522425778404E-4	0.0	0.0	21.25429507818305	0.0
25	2.404522425778404E-4	0.0	0.0	21.564959375593617	0.0
26	2.404522425778404E-4	0.0	0.0	21.833544530553063	0.0
27	2.404522425778404E-4	0.0	0.0	22.15142239524097	0.0
28	2.404522425778404E-4	0.0	0.0	22.494788197642126	0.0
29	2.404522425778404E-4	0.0	0.0	22.80809746972105	0.0
30	2.404522425778404E-4	0.0	0.0	23.256781354371302	0.0
31	2.404522425778404E-4	0.0	0.0	23.54989263807369	0.0
32	2.404522425778404E-4	0.0	0.0	23.87714814022213	0.0
33	2.404522425778404E-4	0.0	0.0	24.21426218431626	0.0
34	2.404522425778404E-4	0.0	0.0	24.51073979941474	0.0
35	2.404522425778404E-4	0.0	0.0	24.855548315271363	0.0
36	2.404522425778404E-4	0.0	0.0	25.180399294994025	0.0
37	2.404522425778404E-4	0.0	0.0	25.487216356523348	0.0
38	7.213567277335212E-4	0.0	0.0	25.81134597951828	0.0
39	7.213567277335212E-4	0.0	0.0	26.17731429272175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.028087E-4	45.000004	31
TATCATG	20	7.028087E-4	45.000004	31
TACCCCA	20	7.028087E-4	45.000004	41
ATCTACG	20	7.028087E-4	45.000004	1
CACGGTT	20	7.028087E-4	45.000004	32
ACGTTTG	20	7.028087E-4	45.000004	1
CTATCTA	20	7.028087E-4	45.000004	21
AGATACG	20	7.028087E-4	45.000004	1
GAATGCG	20	7.028087E-4	45.000004	10
CGACGAA	20	7.028087E-4	45.000004	1
CCCTATC	20	7.028087E-4	45.000004	44
TTACTCG	20	7.028087E-4	45.000004	1
TAACGTT	20	7.028087E-4	45.000004	23
TGTGCGC	30	2.1621472E-6	45.000004	42
CCGTCAA	20	7.028087E-4	45.000004	13
CGTTAGA	20	7.028087E-4	45.000004	44
AAACGTA	20	7.028087E-4	45.000004	31
TGCGCGT	30	2.1621472E-6	45.000004	44
CATAGCG	20	7.028087E-4	45.000004	1
AGCATCG	20	7.028087E-4	45.000004	1
>>END_MODULE