##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487893
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677156671647268	31.0	31.0	34.0	30.0	34.0
2	31.883361720705153	33.0	31.0	34.0	30.0	34.0
3	32.044806955623464	34.0	31.0	34.0	30.0	34.0
4	35.71788281446957	37.0	35.0	37.0	33.0	37.0
5	35.46306054811198	37.0	35.0	37.0	33.0	37.0
6	34.82975980389143	37.0	35.0	37.0	32.0	37.0
7	35.2001381450441	37.0	35.0	37.0	33.0	37.0
8	35.603285966390175	37.0	35.0	37.0	33.0	37.0
9	37.284140170078274	39.0	37.0	39.0	34.0	39.0
10	36.87838112045059	39.0	37.0	39.0	33.0	39.0
11	36.89910287706526	39.0	37.0	39.0	33.0	39.0
12	36.765153425033766	39.0	35.0	39.0	32.0	39.0
13	36.74023197709334	39.0	35.0	39.0	33.0	39.0
14	37.85599096523213	40.0	37.0	41.0	33.0	41.0
15	37.91806400173809	40.0	37.0	41.0	33.0	41.0
16	37.78545705718262	40.0	37.0	41.0	33.0	41.0
17	37.783026196317635	39.0	37.0	41.0	33.0	41.0
18	37.73455860198855	39.0	37.0	41.0	33.0	41.0
19	37.737079646561845	40.0	36.0	41.0	33.0	41.0
20	37.712992397923315	39.0	36.0	41.0	33.0	41.0
21	37.6873802247624	39.0	36.0	41.0	33.0	41.0
22	37.65248732816417	39.0	36.0	41.0	33.0	41.0
23	37.55119667632042	39.0	36.0	41.0	32.0	41.0
24	37.39700303140238	39.0	36.0	41.0	32.0	41.0
25	37.44152918775223	39.0	36.0	41.0	32.0	41.0
26	37.400860434562496	39.0	36.0	41.0	32.0	41.0
27	37.32690364485656	39.0	36.0	41.0	32.0	41.0
28	37.30916409950542	39.0	36.0	41.0	32.0	41.0
29	37.08712976000886	39.0	36.0	41.0	31.0	41.0
30	37.19681979450412	39.0	36.0	41.0	32.0	41.0
31	37.150032896557235	39.0	36.0	41.0	31.0	41.0
32	37.10264340746844	39.0	36.0	41.0	31.0	41.0
33	36.90586050629954	39.0	35.0	41.0	31.0	41.0
34	36.89036530550756	39.0	35.0	41.0	31.0	41.0
35	36.858983424644336	39.0	35.0	41.0	31.0	41.0
36	36.87547679511696	39.0	35.0	40.0	31.0	41.0
37	36.761742841155744	39.0	35.0	40.0	31.0	41.0
38	36.592582799917196	39.0	35.0	40.0	30.0	41.0
39	36.47263231897158	39.0	35.0	40.0	30.0	41.0
40	36.33462050080653	39.0	35.0	40.0	30.0	41.0
41	36.289866835556154	39.0	35.0	40.0	30.0	41.0
42	36.206143560165856	38.0	35.0	40.0	30.0	41.0
43	36.189906393410034	38.0	35.0	40.0	30.0	41.0
44	36.09760541758131	38.0	35.0	40.0	30.0	41.0
45	35.8502376545677	38.0	35.0	40.0	29.0	41.0
46	35.65868130922149	38.0	35.0	40.0	29.0	41.0
47	35.57923561108686	38.0	34.0	40.0	28.0	41.0
48	35.46322451849073	38.0	34.0	40.0	28.0	41.0
49	35.35986374061526	38.0	34.0	40.0	28.0	41.0
50	35.26649695732466	37.0	34.0	40.0	27.0	41.0
51	33.876725019625205	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	7.0
13	6.0
14	18.0
15	19.0
16	41.0
17	99.0
18	176.0
19	365.0
20	560.0
21	932.0
22	1419.0
23	1857.0
24	2311.0
25	2682.0
26	3383.0
27	4131.0
28	5389.0
29	7045.0
30	9191.0
31	12106.0
32	16042.0
33	21691.0
34	32304.0
35	40767.0
36	48179.0
37	67394.0
38	100801.0
39	108947.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.87463029803666	35.643061081015716	19.34973447046791	26.132574150479716
2	21.46946154177248	33.29746481298154	20.837150768713634	24.395922876532357
3	19.89739553549651	30.547476598352507	24.057938933331695	25.497188932819288
4	22.26123350816675	29.892209972268507	19.798808345272427	28.047748174292312
5	17.315886885034217	34.3255590877508	19.86378160785254	28.49477241936244
6	19.76232493600031	35.742468123133555	23.075141475692416	21.420065465173714
7	78.0070630240647	8.212661382721212	7.753544322218191	6.026731270995895
8	79.22741256791961	4.857827433474142	6.365535066090311	9.54922493251594
9	72.79300994275384	8.469889914386966	11.821034530112136	6.916065612747058
10	33.267540218859466	30.71103705115671	16.468364989864583	19.553057740119247
11	24.836388306452438	27.08913634751882	27.573873779701696	20.500601566327042
12	23.966525447177965	24.424412729840355	32.53705218152341	19.07200964145827
13	24.363948652675894	26.09629570418104	27.259460578446504	22.280295064696563
14	18.815600961686272	27.06782019828118	32.354225209215954	21.762353630816595
15	17.858013949779973	29.305196016339647	28.95491429473266	23.881875739147723
16	19.14518142297594	28.549907459217494	28.439022490587075	23.86588862721949
17	18.569030504639336	28.449475602232454	29.361355871061896	23.620138022066314
18	20.995997073128738	26.30945719655744	26.40046075676429	26.29408497354953
19	20.066285025610124	30.152717911509285	29.179758676595075	20.601238386285516
20	22.537318633388878	29.624938254904254	25.104889801657333	22.73285331004954
21	20.718887133039416	28.056766545123622	26.72614692155862	24.498199400278338
22	20.27001822120834	28.579627090366127	26.38057934834072	24.769775340084813
23	22.019787125455785	27.415642364206903	24.4520827312546	26.11248777908271
24	18.793874886501754	29.375703279202614	26.567915506063827	25.262506328231805
25	20.78181076588514	27.3459549532377	25.955281178455113	25.916953102422045
26	22.31493380720773	28.7198217641983	25.626520569059196	23.338723859534774
27	18.201121967316606	26.184224819786305	27.06823012422806	28.54642308866903
28	21.071423447354235	25.57814930732763	28.597868795002185	24.752558450315952
29	20.194181921035963	27.59334526217839	27.04506930822947	25.16740350855618
30	21.06363485436356	24.702752447770308	25.155105730149845	29.078506967716283
31	21.423139909775298	25.62078160580291	25.795410059172813	27.16066842524898
32	20.682608686740743	24.97760779515181	23.996450041300037	30.343333476807416
33	20.078992729963332	23.761972399685995	26.05776266517454	30.101272205176134
34	17.5468801560998	23.762792251579754	30.454013482464394	28.236314109856053
35	18.930995115732344	25.038071872316266	27.492708442219914	28.53822456973148
36	18.856388593400602	23.60456083608496	31.167079667058147	26.371970903456294
37	19.172236535469867	26.142822299151657	23.22988852063875	31.455052644739727
38	20.594269645188596	25.255537587134885	26.176231263822192	27.973961503854326
39	19.97815094703142	23.052185622667267	27.77453253069833	29.19513089960299
40	21.7117277763772	25.91080421321888	26.080923481173123	26.296544529230793
41	18.058877663750046	23.45247830979334	31.77868917979967	26.709954846656952
42	22.22229054321337	25.51727530421629	27.670821266138272	24.589612886432064
43	20.039024950142757	22.923878801294546	28.98442896290785	28.05266728565485
44	21.656797699495588	21.41637613165182	26.268464601869674	30.658361566982922
45	23.849286626370947	22.897028651774058	26.35434408774055	26.89934063411445
46	18.903735040264976	22.07389735044364	32.03714748930606	26.985220119985325
47	20.281086221774036	24.32767020637722	27.065155679626475	28.32608789222227
48	19.70391048857024	22.283369509298147	33.06852117165034	24.944198830481273
49	22.23807269216816	21.722180888022578	28.3953653772446	27.64438104256466
50	20.03533561662086	21.7465714818618	27.25474643005741	30.96334647145993
51	19.98839909570336	20.30527185263982	32.0584636385437	27.64786541311312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2303.0
1	1843.5
2	1384.0
3	939.5
4	495.0
5	451.0
6	407.0
7	419.0
8	431.0
9	452.0
10	473.0
11	521.0
12	569.0
13	693.5
14	818.0
15	785.0
16	752.0
17	806.0
18	860.0
19	945.5
20	1031.0
21	1207.0
22	1383.0
23	1815.0
24	2247.0
25	2409.5
26	2973.0
27	3374.0
28	4705.0
29	6036.0
30	6462.5
31	6889.0
32	7638.0
33	8387.0
34	8976.0
35	9565.0
36	11740.0
37	13915.0
38	14138.5
39	14362.0
40	15628.5
41	16895.0
42	20733.0
43	24571.0
44	34241.0
45	43911.0
46	45823.0
47	47735.0
48	49516.0
49	51297.0
50	49095.5
51	46894.0
52	42386.0
53	37878.0
54	33293.5
55	28709.0
56	26409.0
57	24109.0
58	22216.5
59	20324.0
60	19107.0
61	17890.0
62	16150.5
63	14411.0
64	12358.5
65	10306.0
66	8844.5
67	7383.0
68	6840.5
69	6298.0
70	5029.0
71	3760.0
72	3428.5
73	3097.0
74	2230.5
75	1238.5
76	1113.0
77	989.0
78	865.0
79	550.0
80	235.0
81	249.0
82	263.0
83	207.0
84	151.0
85	155.5
86	160.0
87	86.0
88	12.0
89	8.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487893.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.259744395000673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25128488656527	20.240710470081456
2	11.079879786907055	6.040693818368417
3	4.368670689510144	3.5726653902593357
4	2.152287761030047	2.3468325692066947
5	1.2551165166429143	1.7107077714814727
6	0.8022114677774136	1.3120847737410366
7	0.5496741805583085	1.0488784363785655
8	0.44633868508072994	0.9733662775121119
9	0.3271447424105958	0.8026093852453059
>10	3.258464656707912	21.050852621709996
>50	1.1696458410028203	22.772097256488497
>100	0.3271365071201918	12.04504490084025
>500	0.006072139343298224	1.1659164822852324
>1k	0.0053131219253859455	3.846386407397067
>5k	7.59017417912278E-4	1.0711534390045516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	5177	1.0610933134929175	TruSeq Adapter, Index 16 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGT	4572	0.9370907145624142	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	4461	0.9143398245107022	No Hit
AATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	4019	0.8237461902507311	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1933	0.39619342765729365	No Hit
AAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	1432	0.2935069779644307	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1091	0.2236146040217835	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1082	0.22176993726083383	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	929	0.19041060232469006	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	890	0.18241704636057496	No Hit
AACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	851	0.17442349039645988	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTATC	656	0.13445571057588449	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTAT	644	0.1319961548946183	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	585	0.11990333946172624	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	557	0.11416437620543848	TruSeq Adapter, Index 16 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	523	0.10719563510851765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0496297343884827E-4	0.0	0.0	1.19411428325473	0.0
2	2.0496297343884827E-4	0.0	0.0	1.661634825668743	0.0
3	2.0496297343884827E-4	0.0	0.0	2.328789304212194	0.0
4	2.0496297343884827E-4	0.0	0.0	5.239673452990718	0.0
5	2.0496297343884827E-4	0.0	0.0	5.692026735370256	0.0
6	2.0496297343884827E-4	0.0	0.0	7.632411204915832	0.0
7	2.0496297343884827E-4	0.0	0.0	9.166968987052488	0.0
8	2.0496297343884827E-4	0.0	0.0	10.29160082231145	0.0
9	2.0496297343884827E-4	0.0	0.0	12.618750422736133	0.0
10	2.0496297343884827E-4	0.0	0.0	14.943030541532671	0.0
11	2.0496297343884827E-4	0.0	0.0	17.637063864412895	0.0
12	2.0496297343884827E-4	0.0	0.0	18.60612880283177	0.0
13	2.0496297343884827E-4	0.0	0.0	19.053153047901898	0.0
14	2.0496297343884827E-4	0.0	0.0	19.601019075903938	0.0
15	2.0496297343884827E-4	0.0	0.0	19.980405539739245	0.0
16	2.0496297343884827E-4	0.0	0.0	20.79308372942428	0.0
17	2.0496297343884827E-4	0.0	0.0	21.663356514645628	0.0
18	2.0496297343884827E-4	0.0	0.0	23.094612958169108	0.0
19	2.0496297343884827E-4	0.0	0.0	23.559059055981535	0.0
20	2.0496297343884827E-4	0.0	0.0	24.087863527453766	0.0
21	2.0496297343884827E-4	0.0	0.0	24.63839407411051	0.0
22	2.0496297343884827E-4	0.0	0.0	25.18154595372346	0.0
23	2.0496297343884827E-4	0.0	0.0	25.728182203884867	0.0
24	2.0496297343884827E-4	0.0	0.0	26.144052076992292	0.0
25	2.0496297343884827E-4	0.0	0.0	26.482650909113268	0.0
26	2.0496297343884827E-4	0.0	0.0	26.840926186684374	0.0
27	2.0496297343884827E-4	0.0	0.0	27.246752874093296	0.0
28	2.0496297343884827E-4	0.0	0.0	27.620605337645753	0.0
29	2.0496297343884827E-4	0.0	0.0	28.01105980204676	0.0
30	2.0496297343884827E-4	0.0	0.0	28.55236701489876	0.0
31	2.0496297343884827E-4	0.0	0.0	28.942616516326325	0.0
32	2.0496297343884827E-4	0.0	0.0	29.33491564748828	0.0
33	2.0496297343884827E-4	0.0	0.0	29.700979518050065	0.0
34	2.0496297343884827E-4	0.0	0.0	30.100862279229258	0.0
35	2.0496297343884827E-4	0.0	0.0	30.485782743347414	0.0
36	2.0496297343884827E-4	0.0	0.0	30.821922019787124	0.0
37	2.0496297343884827E-4	0.0	0.0	31.180812186278548	0.0
38	2.0496297343884827E-4	0.0	0.0	31.51162242540885	0.0
39	2.0496297343884827E-4	0.0	0.0	31.889369185456648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCA	30	2.162924E-6	45.000004	36
GTCTCGC	35	1.21001E-7	45.000004	31
CGCGATG	30	2.162924E-6	45.000004	1
CCGCTCA	35	1.21001E-7	45.000004	45
ACCTCGC	30	2.162924E-6	45.000004	25
TCTCGCG	35	1.21001E-7	45.000004	32
CATACTC	35	1.21001E-7	45.000004	43
CTCGCGA	35	1.21001E-7	45.000004	33
CTCGATC	30	2.162924E-6	45.000004	16
TACTACG	30	2.162924E-6	45.000004	1
ACGTACT	30	2.162924E-6	45.000004	24
TCGCGAG	35	1.21001E-7	45.000004	34
ACGACAG	35	1.21001E-7	45.000004	1
TAGAGTC	20	7.02929E-4	45.0	40
TGATAGT	20	7.02929E-4	45.0	33
ACACGTC	25	3.8874943E-5	45.0	11
CGAACGC	20	7.02929E-4	45.0	39
ACACGAT	20	7.02929E-4	45.0	11
CGAACAG	25	3.8874943E-5	45.0	1
ACTGCCG	20	7.02929E-4	45.0	33
>>END_MODULE