##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501644
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67609898653228	31.0	31.0	34.0	30.0	34.0
2	31.886110468778657	33.0	31.0	34.0	30.0	34.0
3	32.02959269920501	33.0	31.0	34.0	30.0	34.0
4	35.69600952069595	37.0	35.0	37.0	33.0	37.0
5	35.466974587556116	37.0	35.0	37.0	33.0	37.0
6	35.48357998899618	37.0	35.0	37.0	33.0	37.0
7	35.530015309661835	37.0	35.0	37.0	33.0	37.0
8	35.5985938235083	37.0	35.0	37.0	35.0	37.0
9	37.31194034016155	39.0	37.0	39.0	34.0	39.0
10	36.88036336525504	39.0	37.0	39.0	32.0	39.0
11	36.91093484622561	39.0	37.0	39.0	33.0	39.0
12	36.81056087584024	39.0	35.0	39.0	33.0	39.0
13	36.78497300874724	39.0	35.0	39.0	33.0	39.0
14	37.88812185533964	40.0	37.0	41.0	33.0	41.0
15	37.954274345950516	40.0	37.0	41.0	33.0	41.0
16	37.82252752948306	40.0	37.0	41.0	33.0	41.0
17	37.79386975624148	39.0	37.0	41.0	33.0	41.0
18	37.70867986061829	39.0	36.0	41.0	33.0	41.0
19	37.72791860363126	39.0	36.0	41.0	33.0	41.0
20	37.70867587372719	39.0	36.0	41.0	33.0	41.0
21	37.67422714115987	39.0	36.0	41.0	33.0	41.0
22	37.63535495291482	39.0	36.0	41.0	33.0	41.0
23	37.49802648890448	39.0	36.0	41.0	32.0	41.0
24	37.356449992424906	39.0	36.0	41.0	32.0	41.0
25	37.39992703989283	39.0	36.0	41.0	32.0	41.0
26	37.35770985001316	39.0	36.0	41.0	32.0	41.0
27	37.32349634402086	39.0	36.0	41.0	32.0	41.0
28	37.27479048887259	39.0	36.0	41.0	32.0	41.0
29	37.10631045123633	39.0	36.0	41.0	31.0	41.0
30	37.200131966095476	39.0	36.0	41.0	32.0	41.0
31	37.12715591136344	39.0	36.0	41.0	31.0	41.0
32	37.068911419253496	39.0	36.0	41.0	31.0	41.0
33	37.035684668808955	39.0	36.0	41.0	31.0	41.0
34	36.95134198754495	39.0	35.0	41.0	31.0	41.0
35	36.87186530687101	39.0	35.0	41.0	31.0	41.0
36	36.86364633086412	39.0	35.0	40.0	31.0	41.0
37	36.73858154388371	39.0	35.0	40.0	31.0	41.0
38	36.671789157250956	39.0	35.0	40.0	31.0	41.0
39	36.49188069627066	39.0	35.0	40.0	30.0	41.0
40	36.30872491248774	39.0	35.0	40.0	30.0	41.0
41	36.22716508121297	39.0	35.0	40.0	30.0	41.0
42	36.11575738970266	38.0	35.0	40.0	30.0	41.0
43	36.07524060887801	38.0	35.0	40.0	30.0	41.0
44	36.01569639026879	38.0	35.0	40.0	30.0	41.0
45	35.78202868966837	38.0	35.0	40.0	29.0	41.0
46	35.58563842087217	38.0	34.0	40.0	28.0	41.0
47	35.45406902105876	38.0	34.0	40.0	28.0	41.0
48	35.371859326534356	38.0	34.0	40.0	28.0	41.0
49	35.206983438454365	38.0	34.0	40.0	27.0	41.0
50	35.10968535455422	37.0	34.0	40.0	27.0	41.0
51	33.64947452775275	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	7.0
11	4.0
12	6.0
13	6.0
14	10.0
15	21.0
16	44.0
17	114.0
18	177.0
19	356.0
20	582.0
21	900.0
22	1347.0
23	1898.0
24	2338.0
25	2941.0
26	3579.0
27	4535.0
28	5520.0
29	7303.0
30	9692.0
31	12591.0
32	16475.0
33	22187.0
34	32914.0
35	41811.0
36	48485.0
37	68747.0
38	104795.0
39	112237.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.805766639290013	35.9141143918795	19.086443772874787	26.193675195955695
2	21.329269362336635	33.597730661584706	20.823332881485676	24.24966709459298
3	19.85950195756353	30.49014839208682	24.19046176172744	25.459887888622212
4	22.329380995287494	29.780083086810567	19.826610105971564	28.063925811930375
5	17.095789045618005	34.416837438502206	19.954788654902682	28.532584860977106
6	20.203570659671	34.34387733133457	23.6930970967459	21.759454912248525
7	77.32575292438463	8.311471880457058	8.179904474089195	6.182870721069125
8	78.5746465621038	4.886931768345679	6.584151310491106	9.954270359059413
9	72.27535862085463	8.416925150106449	12.337633859868752	6.970082369170169
10	33.035379671639646	30.558922263597292	16.56652925182002	19.839168812943043
11	24.578784955067736	26.96812879253016	27.965648946264682	20.48743730613742
12	23.590434650867948	24.31903899976876	33.06767348956631	19.022852859796988
13	24.324221958201434	26.153806284935133	27.13039526038386	22.391576496479576
14	18.532664598799148	27.111258183093987	32.72400347656904	21.632073741537823
15	17.546706429260592	29.593496583234323	29.017988852652476	23.841808134852606
16	19.061525703486932	28.355965585156007	28.635645995965263	23.94686271539179
17	18.264546172185852	28.68229262185933	29.47109902640119	23.582062179553628
18	20.647510983884988	26.332020317197056	26.517410753442682	26.503057945475277
19	19.67132069754647	30.364760666927143	29.388769725143725	20.575148910382662
20	22.404932581671464	29.65090781510394	25.314765052507354	22.62939455071724
21	20.395738810790125	28.04060249898334	26.711373005557725	24.85228568466881
22	19.985089027278306	28.557901619475167	26.83815614260312	24.618853210643405
23	22.126049549082616	27.185215013037134	24.36708103754854	26.321654400331713
24	18.44933857476617	29.661074387414182	26.545917024822387	25.343670012997265
25	20.71548747717505	26.936632352823914	26.078454043106262	26.269426126894768
26	22.39038042914896	28.711994960569648	25.545606047316422	23.35201856296497
27	17.937023068151916	25.909609204934174	27.397915653331843	28.75545207358206
28	20.99556657709451	25.38652909234437	28.715583162561497	24.902321167999617
29	20.006618239229415	27.450941304989197	27.247410514229216	25.295029941552176
30	20.98260918101283	24.57180789563914	25.057610576424715	29.387972346923313
31	21.175774054907464	25.543612601765396	25.8974491870729	27.383164156254235
32	20.383977482039057	24.890360494693446	23.971182751114338	30.754479272153162
33	19.94342601526182	23.484582692108347	26.069882227236846	30.50210906539299
34	17.26622864023092	23.521860123912575	30.65959126392422	28.552319971932288
35	18.676591367583388	25.170439594612915	27.430209471258504	28.722759566545196
36	18.59426206632592	23.29540470931577	31.53491320538071	26.575420018977603
37	18.969627863584535	26.022637567677474	23.133138241462074	31.874596327275917
38	20.44637232778624	25.15050513910263	26.182511900869937	28.22061063224119
39	19.704212549138433	22.830134517705783	28.141271499310268	29.324381433845513
40	21.56529331557838	26.114336063024773	25.97140601701605	26.348964604380797
41	17.985862484152108	23.2270295269155	32.159061007407644	26.628046981524744
42	22.267982872315827	25.53025651657351	27.66822687005127	24.533533741059397
43	19.96056964700066	22.772125252170863	29.076994840962918	28.190310259865566
44	21.57246971956208	21.24115906898119	26.274808429882548	30.911562781574187
45	24.04414285828197	22.633780130929505	26.33560851918891	26.98646849159962
46	18.63652311200772	21.86989179577549	32.39428758242897	27.099297509787817
47	20.176459800177017	24.204216536029534	26.979890121281226	28.639433542512222
48	19.529985407978568	22.23170216328711	33.31067450223665	24.927637926497674
49	22.117477733213196	21.53638835508847	28.565476712569073	27.780657199129262
50	19.86508360510641	21.526819816443535	27.267344969739497	31.34075160871056
51	19.83059699707362	20.04668649480508	32.293817926657155	27.828898581464145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2348.0
1	1902.5
2	1457.0
3	1003.0
4	549.0
5	492.5
6	436.0
7	441.0
8	446.0
9	465.5
10	485.0
11	522.0
12	559.0
13	710.0
14	861.0
15	795.5
16	730.0
17	824.0
18	918.0
19	972.0
20	1026.0
21	1189.0
22	1352.0
23	1773.5
24	2195.0
25	2487.5
26	3102.0
27	3424.0
28	4846.0
29	6268.0
30	6647.5
31	7027.0
32	7813.0
33	8599.0
34	9166.0
35	9733.0
36	11917.5
37	14102.0
38	14179.5
39	14257.0
40	15625.5
41	16994.0
42	21077.5
43	25161.0
44	35868.0
45	46575.0
46	48354.0
47	50133.0
48	51825.5
49	53518.0
50	50969.5
51	48421.0
52	43778.5
53	39136.0
54	34337.0
55	29538.0
56	26899.5
57	24261.0
58	22241.0
59	20221.0
60	19216.0
61	18211.0
62	16550.5
63	14890.0
64	12661.0
65	10432.0
66	8876.5
67	7321.0
68	6787.5
69	6254.0
70	4990.5
71	3727.0
72	3370.5
73	3014.0
74	2207.5
75	1281.0
76	1161.0
77	1029.5
78	898.0
79	565.0
80	232.0
81	244.5
82	257.0
83	198.5
84	140.0
85	152.5
86	165.0
87	89.0
88	13.0
89	9.5
90	6.0
91	3.5
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	501644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.96512056516367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38561238500327	20.05817006275145
2	10.954964282165703	5.908038653113197
3	4.340769182853986	3.5114809308361417
4	2.1953844445369604	2.3679522493529603
5	1.2184425237797174	1.6427724777721187
6	0.8430377726469805	1.3639569108247722
7	0.5156804588556202	0.9733770022298506
8	0.43966284071699036	0.9484449206364796
9	0.35259249030645434	0.855692911033636
>10	3.246844191589373	20.728737956347704
>50	1.1633577219547122	22.702190820641857
>100	0.3317503478208451	12.478031480699304
>500	0.004463009163509127	0.8259718319512582
>1k	0.0059506788846788354	3.5321427782082706
>5k	0.0014876697211697089	2.103039013601006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	5435	1.083437656983837	TruSeq Adapter, Index 16 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGT	5050	1.0066900032692507	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	4992	0.9951280190732871	No Hit
AATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	4518	0.9006386999545495	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1951	0.38892122700560555	No Hit
AAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	1627	0.32433359115229127	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1226	0.2443964245560597	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1222	0.24359904633564836	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1045	0.20831506008244888	TruSeq Adapter, Index 13 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	1029	0.20512554720080378	No Hit
AACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	932	0.18578912535583003	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTAT	695	0.13854446579646124	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	676	0.1347569192495076	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTATC	664	0.13236478458827375	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	579	0.1154204974045339	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	572	0.11402508551881414	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9934455510282192E-4	0.0	0.0	1.2612530001355542	0.0
2	1.9934455510282192E-4	0.0	0.0	1.7510425720231877	0.0
3	1.9934455510282192E-4	0.0	0.0	2.470477071389272	0.0
4	1.9934455510282192E-4	0.0	0.0	5.688894913524332	0.0
5	1.9934455510282192E-4	0.0	0.0	6.154563794244524	0.0
6	1.9934455510282192E-4	0.0	0.0	8.242099975281276	0.0
7	1.9934455510282192E-4	0.0	0.0	9.85659152705903	0.0
8	1.9934455510282192E-4	0.0	0.0	10.993453524810423	0.0
9	1.9934455510282192E-4	0.0	0.0	13.418878726746458	0.0
10	1.9934455510282192E-4	0.0	0.0	15.78350383937613	0.0
11	1.9934455510282192E-4	0.0	0.0	18.63233687635056	0.0
12	1.9934455510282192E-4	0.0	0.0	19.613710121121752	0.0
13	1.9934455510282192E-4	0.0	0.0	20.06004257999697	0.0
14	1.9934455510282192E-4	0.0	0.0	20.621994880831824	0.0
15	1.9934455510282192E-4	0.0	0.0	21.03344204256405	0.0
16	1.9934455510282192E-4	0.0	0.0	21.82184975799571	0.0
17	1.9934455510282192E-4	0.0	0.0	22.72687403816252	0.0
18	1.9934455510282192E-4	0.0	0.0	24.20022964492748	0.0
19	1.9934455510282192E-4	0.0	0.0	24.68782642670898	0.0
20	1.9934455510282192E-4	0.0	0.0	25.232037062139685	0.0
21	1.9934455510282192E-4	0.0	0.0	25.8264825254563	0.0
22	1.9934455510282192E-4	0.0	0.0	26.407571903581026	0.0
23	1.9934455510282192E-4	0.0	0.0	26.962945834097486	0.0
24	1.9934455510282192E-4	0.0	0.0	27.388945148352217	0.0
25	1.9934455510282192E-4	0.0	0.0	27.740389598998494	0.0
26	1.9934455510282192E-4	0.0	0.0	28.094425528861105	0.0
27	1.9934455510282192E-4	0.0	0.0	28.487931680634077	0.0
28	1.9934455510282192E-4	0.0	0.0	28.87466011753355	0.0
29	1.9934455510282192E-4	0.0	0.0	29.262783966318743	0.0
30	1.9934455510282192E-4	0.0	0.0	29.80938673641068	0.0
31	1.9934455510282192E-4	0.0	0.0	30.200301408967317	0.0
32	1.9934455510282192E-4	0.0	0.0	30.60058527561378	0.0
33	1.9934455510282192E-4	0.0	0.0	30.9833268214112	0.0
34	1.9934455510282192E-4	0.0	0.0	31.38540478905359	0.0
35	1.9934455510282192E-4	0.0	0.0	31.781701764598	0.0
36	1.9934455510282192E-4	0.0	0.0	32.12278029837893	0.0
37	1.9934455510282192E-4	0.0	0.0	32.47581950546603	0.0
38	1.9934455510282192E-4	0.0	0.0	32.810319668928564	0.0
39	1.9934455510282192E-4	0.0	0.0	33.19924089593417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGT	20	7.029479E-4	45.000004	19
GTACCGG	20	7.029479E-4	45.000004	2
TCCGCGC	20	7.029479E-4	45.000004	27
AATTTCG	30	2.1630458E-6	45.000004	39
CGACGTT	30	2.1630458E-6	45.000004	24
CTTCCGA	30	2.1630458E-6	45.000004	21
CCGATCT	20	7.029479E-4	45.000004	21
CGGCCGT	30	2.1630458E-6	45.000004	41
TCCGACT	30	2.1630458E-6	45.000004	23
GCTATTC	20	7.029479E-4	45.000004	7
GATCCGA	20	7.029479E-4	45.000004	10
CACGCTA	20	7.029479E-4	45.000004	41
TAAACCG	20	7.029479E-4	45.000004	1
ATTCGTT	20	7.029479E-4	45.000004	41
TCCTCGA	20	7.029479E-4	45.000004	10
TAAGTGT	30	2.1630458E-6	45.000004	14
CGACAGT	20	7.029479E-4	45.000004	2
TGTGCTA	30	2.1630458E-6	45.000004	20
GCGTTGC	20	7.029479E-4	45.000004	39
CCGTCTA	20	7.029479E-4	45.000004	37
>>END_MODULE