SRR3553206_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548879
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	5121	0.9329925174765293	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	4622	0.8420799484039287	No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	4474	0.8151158998613538	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	4147	0.7555399277436375	RNA PCR Primer, Index 16 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1433	0.2610775781183102	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1290	0.23502447716163308	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	980	0.1785457268359693	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	913	0.16633902918493876	RNA PCR Primer, Index 14 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	839	0.15285700491365128	RNA PCR Primer, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	776	0.14137906533133895	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	775	0.1411968758141594	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	768	0.13992154919390248	RNA PCR Primer, Index 16 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACTC	719	0.13099426285210403	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	682	0.12425325071646028	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	617	0.11241093209978885	No Hit
TGCCACGGGAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTC	615	0.11204655306542971	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAT	35	1.2103737E-7	45.000004	43
TTCACGG	65	0.0	45.000004	2
CGCGACC	30	2.1634205E-6	45.000004	10
CATAACG	30	2.1634205E-6	45.000004	1
TAGATAG	30	2.1634205E-6	45.000004	1
CTCGACG	35	1.2103737E-7	45.000004	1
GCCAACG	30	2.1634205E-6	45.000004	1
AACGTAA	20	7.0300617E-4	45.0	35
GGTACGA	20	7.0300617E-4	45.0	9
TCCGCAA	25	3.8881335E-5	45.0	43
CTATCAC	20	7.0300617E-4	45.0	37
GCACGTT	25	3.8881335E-5	45.0	45
ATTCGCC	20	7.0300617E-4	45.0	23
TACGGTT	20	7.0300617E-4	45.0	33
TCGATTG	25	3.8881335E-5	45.0	12
TCGATAG	20	7.0300617E-4	45.0	1
CCCGTAC	20	7.0300617E-4	45.0	31
CTACGTT	20	7.0300617E-4	45.0	35
AACATAG	25	3.8881335E-5	45.0	1
GTCCGCG	25	3.8881335E-5	45.0	23