##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548879
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711227793375226	31.0	31.0	34.0	30.0	34.0
2	31.926058384452677	33.0	31.0	34.0	30.0	34.0
3	32.07269908304016	34.0	31.0	34.0	30.0	34.0
4	35.74428061558194	37.0	35.0	37.0	35.0	37.0
5	35.49524212075885	37.0	35.0	37.0	33.0	37.0
6	34.84929647517941	37.0	35.0	37.0	32.0	37.0
7	35.20913716866559	37.0	35.0	37.0	33.0	37.0
8	35.625531310179475	37.0	35.0	37.0	33.0	37.0
9	37.309847889972104	39.0	37.0	39.0	34.0	39.0
10	36.89893400913498	39.0	37.0	39.0	33.0	39.0
11	36.917903581663715	39.0	37.0	39.0	33.0	39.0
12	36.732166834584675	39.0	35.0	39.0	32.0	39.0
13	36.72779064238202	39.0	35.0	39.0	33.0	39.0
14	37.83741954055448	40.0	37.0	41.0	33.0	41.0
15	37.89549062726029	40.0	37.0	41.0	33.0	41.0
16	37.76128254132514	40.0	36.0	41.0	33.0	41.0
17	37.75991065426078	39.0	36.0	41.0	33.0	41.0
18	37.69861116931054	39.0	36.0	41.0	33.0	41.0
19	37.706060898668014	40.0	36.0	41.0	32.0	41.0
20	37.66634540581804	39.0	36.0	41.0	33.0	41.0
21	37.67926264258607	39.0	36.0	41.0	33.0	41.0
22	37.656926207779854	39.0	36.0	41.0	33.0	41.0
23	37.57074327857324	39.0	36.0	41.0	32.0	41.0
24	37.437792300306626	39.0	36.0	41.0	32.0	41.0
25	37.44729712741788	39.0	36.0	41.0	32.0	41.0
26	37.42932413154812	39.0	36.0	41.0	32.0	41.0
27	37.33457100745337	39.0	36.0	41.0	32.0	41.0
28	37.25254564302879	39.0	36.0	41.0	32.0	41.0
29	37.042211489235335	39.0	35.0	41.0	31.0	41.0
30	37.04731461761153	39.0	35.0	41.0	31.0	41.0
31	37.01485937702117	39.0	35.0	41.0	31.0	41.0
32	36.97461553457137	39.0	35.0	41.0	31.0	41.0
33	36.651680971580255	39.0	35.0	40.0	30.0	41.0
34	36.71385132242261	39.0	35.0	40.0	30.0	41.0
35	36.63620943778137	39.0	35.0	40.0	30.0	41.0
36	36.6521819927525	39.0	35.0	40.0	30.0	41.0
37	36.57277286979462	39.0	35.0	40.0	30.0	41.0
38	36.33506109725459	39.0	35.0	40.0	30.0	41.0
39	36.27546508428998	39.0	35.0	40.0	30.0	41.0
40	36.12228378203575	38.0	35.0	40.0	30.0	41.0
41	36.18850967153052	38.0	35.0	40.0	30.0	41.0
42	36.195015659189	38.0	35.0	40.0	30.0	41.0
43	36.135159115214826	38.0	35.0	40.0	30.0	41.0
44	36.063332719961956	38.0	35.0	40.0	30.0	41.0
45	35.834870709209135	38.0	35.0	40.0	29.0	41.0
46	35.60608075732539	38.0	34.0	40.0	28.0	41.0
47	35.518547803796466	38.0	34.0	40.0	28.0	41.0
48	35.498692790214236	38.0	34.0	40.0	28.0	41.0
49	35.405555687136875	38.0	34.0	40.0	28.0	41.0
50	35.34875810515614	38.0	34.0	40.0	28.0	41.0
51	34.01224860123998	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	0.0
12	9.0
13	14.0
14	11.0
15	20.0
16	54.0
17	86.0
18	167.0
19	375.0
20	685.0
21	1153.0
22	1589.0
23	2139.0
24	2459.0
25	3048.0
26	3751.0
27	4771.0
28	6038.0
29	8025.0
30	10694.0
31	14109.0
32	18552.0
33	25277.0
34	38069.0
35	46467.0
36	53935.0
37	75710.0
38	109348.0
39	122283.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.15321956205284	33.98599691370958	21.237103259552654	25.62368026468493
2	23.383295771927877	33.64603127465252	19.091275126211787	23.879397827207818
3	20.527110711103905	28.610495209326647	23.553096401939225	27.309297677630223
4	23.199466549093696	29.93610613632513	18.67679397462829	28.187633339952882
5	18.006518740924683	36.08901051051325	17.6257426500194	28.278728098542665
6	19.66225707305253	36.125630603466334	21.053638415752836	23.158473907728297
7	79.0788133632367	8.33261975772438	7.414020212105036	5.174546666933878
8	80.62560236409118	4.8473342940793875	5.562974717560701	8.964088624268737
9	75.51300013299834	7.94710673937243	10.280225696373883	6.25966743125534
10	34.84228764445351	32.45287212664358	14.581355817948946	18.123484410953964
11	27.044394119651145	27.575294372712385	25.635158204267245	19.745153303369232
12	25.90607401631325	22.39710391543491	32.52884515530746	19.167976912944383
13	26.49764337859528	25.845951475643997	25.747386946849854	21.90901819891087
14	19.89983220345468	26.898278126873137	31.524616536613713	21.67727313305847
15	18.676611785111106	29.1109698130189	29.0521225989699	23.160295802900094
16	20.780536329500674	27.54723718706673	28.41464147835862	23.257585005073977
17	20.169108309846067	27.508066440873126	27.820885841870428	24.501939407410376
18	22.769134909515575	25.889859149284266	26.059841968812798	25.281163972387354
19	22.289794289816154	29.741163352943	27.64707704248113	20.32196531475972
20	23.694657656787744	29.534013871909835	25.917369766378386	20.853958704924036
21	22.905594857883067	27.790095813467087	26.022310928273807	23.28199840037604
22	20.920275689177394	28.04661865365591	26.702424395905112	24.33068126126159
23	21.405628562943747	26.958947236093927	25.45424401370794	26.181180187254387
24	19.882888578356976	29.613266311882946	26.040165500957407	24.463679608802668
25	21.95802717903217	27.988318008158448	25.82827909247758	24.225375720331805
26	23.168676520690354	26.964959490160854	23.926220533122965	25.940143456025826
27	19.29478081690136	26.82959267889644	27.426263347659503	26.4493631565427
28	20.910984023801237	25.812610794000136	28.766631625549525	24.509773556649098
29	21.01392110100769	28.72982934307926	24.303352833684656	25.952896722228395
30	22.123819639665573	27.747099087412707	25.72880361609754	24.400277656824183
31	22.223477305562792	28.317898844736273	23.22005396453499	26.238569885165948
32	21.593283765638695	26.730663771067942	23.198373411990623	28.477679051302747
33	20.792014269082987	28.652034419243588	24.954680357601582	25.601270954071843
34	18.645457377673402	27.173384297814273	25.8022259915209	28.378932332991425
35	19.014573339479192	27.301281338874322	26.468493055846555	27.21565226579993
36	20.445307617890283	29.68887496151246	25.277884561078125	24.587932859519128
37	19.337777542955735	30.676342144625686	20.75393665999246	29.23194365242613
38	21.575429192955095	28.25777630406702	24.55805377870168	25.608740724276206
39	20.12228560393092	26.69331492004613	25.31705530727173	27.867344168751217
40	21.720452048630026	27.86370037840763	24.209160853302823	26.206686719659523
41	19.338506301024452	26.280291284600064	27.88884253177841	26.49235988259708
42	22.125459345320188	26.095004545628452	24.64860196873992	27.130934140311435
43	20.34656089957896	24.792167308277417	27.048402289029095	27.81286950311453
44	21.57378948730048	22.806119381503027	26.08042938425409	29.539661746942404
45	24.381512136554687	24.262360192319253	25.77690164863294	25.579226022493117
46	19.14447446522822	24.974721204491335	29.859039970558175	26.021764359722273
47	20.382998803014875	27.426263347659503	24.72384623933508	27.466891609990547
48	19.33468032116368	24.873059453905142	29.92553914432871	25.86672108060246
49	21.114671904007988	24.12790432864074	28.341036913418076	26.4163868539332
50	20.55115972737161	23.33647306601273	26.897002800252878	29.215364406362788
51	19.925156546342638	21.82284255728494	30.873471202213963	27.37852969415846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1880.0
1	1442.0
2	1004.0
3	695.5
4	387.0
5	360.0
6	333.0
7	389.0
8	445.0
9	448.5
10	452.0
11	493.5
12	535.0
13	603.5
14	672.0
15	740.0
16	808.0
17	987.0
18	1166.0
19	1070.0
20	974.0
21	1144.5
22	1315.0
23	2145.5
24	2976.0
25	2910.5
26	3699.5
27	4554.0
28	5428.5
29	6303.0
30	7685.5
31	9068.0
32	9798.5
33	10529.0
34	12028.0
35	13527.0
36	13832.5
37	14138.0
38	14977.5
39	15817.0
40	18154.0
41	20491.0
42	23330.0
43	26169.0
44	37210.0
45	48251.0
46	49322.0
47	50393.0
48	54106.0
49	57819.0
50	53979.0
51	50139.0
52	45896.5
53	41654.0
54	37414.5
55	33175.0
56	30063.0
57	26951.0
58	25120.0
59	23289.0
60	22144.0
61	20999.0
62	19478.5
63	17958.0
64	15677.5
65	13397.0
66	11457.5
67	9518.0
68	7978.0
69	6438.0
70	5417.0
71	4396.0
72	3745.0
73	3094.0
74	2771.5
75	1922.0
76	1395.0
77	1047.5
78	700.0
79	485.0
80	270.0
81	185.5
82	101.0
83	66.0
84	31.0
85	34.5
86	38.0
87	26.0
88	14.0
89	13.5
90	13.0
91	7.5
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	548879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.21717938770865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8659579424042	20.64857386738775
2	10.198488382391782	5.5514817557403955
3	3.630330350814543	2.9642205718428816
4	1.71691646368741	1.8691849354356243
5	1.0378625641838106	1.412384579458903
6	0.6769017110893186	1.1054013179138953
7	0.4754606484957212	0.9058488433333034
8	0.36802490971866786	0.8013279989566613
9	0.3086200803259366	0.7559791279992062
>10	4.261799102749475	28.67471205139006
>50	1.3050238494496642	23.964618926833047
>100	0.14371609198010393	6.012658969250132
>500	0.006811189193369855	1.4244703557907756
>1k	0.0034055945966849277	2.9597987637370538
>5k	6.811189193369855E-4	0.9493379349303178
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	5121	0.9329925174765293	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	4622	0.8420799484039287	No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	4474	0.8151158998613538	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	4147	0.7555399277436375	RNA PCR Primer, Index 16 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1433	0.2610775781183102	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1290	0.23502447716163308	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	980	0.1785457268359693	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	913	0.16633902918493876	RNA PCR Primer, Index 14 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	839	0.15285700491365128	RNA PCR Primer, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	776	0.14137906533133895	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	775	0.1411968758141594	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	768	0.13992154919390248	RNA PCR Primer, Index 16 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACTC	719	0.13099426285210403	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	682	0.12425325071646028	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	617	0.11241093209978885	No Hit
TGCCACGGGAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTC	615	0.11204655306542971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6437903435912103E-4	0.0	0.0	0.8737809243931722	0.0
2	3.6437903435912103E-4	0.0	0.0	1.2228560393092103	0.0
3	3.6437903435912103E-4	0.0	0.0	1.7326223083776207	0.0
4	3.6437903435912103E-4	0.0	0.0	4.225339282428368	0.0
5	3.6437903435912103E-4	0.0	0.0	4.523583522051308	0.0
6	3.6437903435912103E-4	0.0	0.0	6.034481188021403	0.0
7	3.6437903435912103E-4	0.0	0.0	7.190109295491356	0.0
8	3.6437903435912103E-4	0.0	0.0	7.941094485305505	0.0
9	3.6437903435912103E-4	0.0	0.0	9.980159561579146	0.0
10	3.6437903435912103E-4	0.0	0.0	11.593812115238514	0.0
11	3.6437903435912103E-4	0.0	0.0	13.91672845927791	0.0
12	3.6437903435912103E-4	0.0	0.0	14.726925242175415	0.0
13	3.6437903435912103E-4	0.0	0.0	15.116810808939675	0.0
14	3.6437903435912103E-4	0.0	0.0	15.618014170700647	0.0
15	3.6437903435912103E-4	0.0	0.0	15.988769838161051	0.0
16	3.6437903435912103E-4	0.0	0.0	16.664692946897222	0.0
17	3.6437903435912103E-4	0.0	0.0	17.520983677641155	0.0
18	3.6437903435912103E-4	0.0	0.0	18.807606047963212	0.0
19	3.6437903435912103E-4	0.0	0.0	19.25688539732801	0.0
20	3.6437903435912103E-4	0.0	0.0	19.830600186926446	0.0
21	3.6437903435912103E-4	0.0	0.0	20.36350452467666	0.0
22	3.6437903435912103E-4	0.0	0.0	20.921733205314833	0.0
23	3.6437903435912103E-4	0.0	0.0	21.464111397958384	0.0
24	3.6437903435912103E-4	0.0	0.0	21.889159541538298	0.0
25	3.6437903435912103E-4	0.0	0.0	22.24515785810716	0.0
26	3.6437903435912103E-4	0.0	0.0	22.55524441634677	0.0
27	3.6437903435912103E-4	0.0	0.0	22.95770105979642	0.0
28	3.6437903435912103E-4	0.0	0.0	23.330278622428622	0.0
29	3.6437903435912103E-4	0.0	0.0	23.694475467270564	0.0
30	3.6437903435912103E-4	0.0	0.0	24.265457414111307	0.0
31	3.6437903435912103E-4	0.0	0.0	24.600868315238877	0.0
32	3.6437903435912103E-4	0.0	0.0	24.9563200632562	0.0
33	3.6437903435912103E-4	0.0	0.0	25.311954000790703	0.0
34	3.6437903435912103E-4	0.0	0.0	25.659935978603663	0.0
35	3.6437903435912103E-4	0.0	0.0	26.029234129926632	0.0
36	3.6437903435912103E-4	0.0	0.0	26.364645031054202	0.0
37	3.6437903435912103E-4	0.0	0.0	26.70151344831921	0.0
38	3.6437903435912103E-4	0.0	0.0	27.04530506723704	0.0
39	3.6437903435912103E-4	0.0	0.0	27.419886714558217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAT	35	1.2103737E-7	45.000004	43
TTCACGG	65	0.0	45.000004	2
CGCGACC	30	2.1634205E-6	45.000004	10
CATAACG	30	2.1634205E-6	45.000004	1
TAGATAG	30	2.1634205E-6	45.000004	1
CTCGACG	35	1.2103737E-7	45.000004	1
GCCAACG	30	2.1634205E-6	45.000004	1
AACGTAA	20	7.0300617E-4	45.0	35
GGTACGA	20	7.0300617E-4	45.0	9
TCCGCAA	25	3.8881335E-5	45.0	43
CTATCAC	20	7.0300617E-4	45.0	37
GCACGTT	25	3.8881335E-5	45.0	45
ATTCGCC	20	7.0300617E-4	45.0	23
TACGGTT	20	7.0300617E-4	45.0	33
TCGATTG	25	3.8881335E-5	45.0	12
TCGATAG	20	7.0300617E-4	45.0	1
CCCGTAC	20	7.0300617E-4	45.0	31
CTACGTT	20	7.0300617E-4	45.0	35
AACATAG	25	3.8881335E-5	45.0	1
GTCCGCG	25	3.8881335E-5	45.0	23
>>END_MODULE