SRR3553205_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562746
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	5734	1.018932164777715	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	5396	0.9588695432752965	No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	5068	0.9005839224090442	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	4551	0.8087129895192502	RNA PCR Primer, Index 16 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1427	0.2535779907809207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1387	0.2464699882362558	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	1067	0.1896059678789365	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1025	0.18214256520703834	RNA PCR Primer, Index 14 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	989	0.17574536291683993	RNA PCR Primer, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	831	0.14766875286541353	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	819	0.14553635210201404	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	815	0.14482555184754756	RNA PCR Primer, Index 16 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACTC	771	0.13700674904841614	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	735	0.13060954675821773	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	686	0.12190224364100322	No Hit
TGCCACGGGAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTC	673	0.11959214281398711	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTA	20	7.030212E-4	45.000004	44
CGGGTAT	100	0.0	45.000004	6
AGCCGTA	20	7.030212E-4	45.000004	32
GTCGCTC	25	3.888258E-5	45.000004	9
AATCCGA	25	3.888258E-5	45.000004	24
ATCTCGC	20	7.030212E-4	45.000004	23
TACCACG	20	7.030212E-4	45.000004	1
AGATACG	25	3.888258E-5	45.000004	1
CGAAACG	25	3.888258E-5	45.000004	1
GCGCGAC	60	0.0	45.000004	9
TTTCGCA	30	2.1635187E-6	45.000004	14
CATTGCG	20	7.030212E-4	45.000004	1
CGACCAT	20	7.030212E-4	45.000004	12
ATTGTCG	25	3.888258E-5	45.000004	41
TTCGTCC	20	7.030212E-4	45.000004	36
ATTCGCC	20	7.030212E-4	45.000004	23
CGCGACC	60	0.0	45.000004	10
CGTTCCA	20	7.030212E-4	45.000004	36
GCACCTT	20	7.030212E-4	45.000004	24
ACCTATC	20	7.030212E-4	45.000004	27