##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562746
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718702576295524	31.0	31.0	34.0	30.0	34.0
2	31.924752908061542	33.0	31.0	34.0	30.0	34.0
3	32.054589459543024	33.0	31.0	34.0	30.0	34.0
4	35.73562139935246	37.0	35.0	37.0	33.0	37.0
5	35.49634826369268	37.0	35.0	37.0	33.0	37.0
6	35.52527072604692	37.0	35.0	37.0	33.0	37.0
7	35.544586722962045	37.0	35.0	37.0	33.0	37.0
8	35.63274372452226	37.0	35.0	37.0	35.0	37.0
9	37.332009467859386	39.0	37.0	39.0	34.0	39.0
10	36.90902645243147	39.0	37.0	39.0	33.0	39.0
11	36.929705408834536	39.0	37.0	39.0	33.0	39.0
12	36.770365315790784	39.0	35.0	39.0	33.0	39.0
13	36.7697469195694	39.0	35.0	39.0	33.0	39.0
14	37.857203782878955	40.0	37.0	41.0	33.0	41.0
15	37.91824020072999	40.0	37.0	41.0	33.0	41.0
16	37.79596834095667	40.0	37.0	41.0	33.0	41.0
17	37.76875535321442	40.0	37.0	41.0	33.0	41.0
18	37.67133307033724	39.0	36.0	41.0	33.0	41.0
19	37.70885266176925	40.0	36.0	41.0	32.0	41.0
20	37.67819229279284	39.0	36.0	41.0	33.0	41.0
21	37.68875656157485	39.0	36.0	41.0	33.0	41.0
22	37.6478251289214	39.0	36.0	41.0	33.0	41.0
23	37.524972189940044	39.0	36.0	41.0	32.0	41.0
24	37.41047648495058	39.0	36.0	41.0	32.0	41.0
25	37.41598874092397	39.0	36.0	41.0	32.0	41.0
26	37.37984632498498	39.0	36.0	41.0	32.0	41.0
27	37.33647507045808	39.0	36.0	41.0	32.0	41.0
28	37.23862808442885	39.0	36.0	41.0	32.0	41.0
29	37.07293343711017	39.0	35.0	41.0	31.0	41.0
30	37.047733791088696	39.0	35.0	41.0	31.0	41.0
31	36.996286068670415	39.0	35.0	41.0	31.0	41.0
32	36.926288236611185	39.0	35.0	40.0	31.0	41.0
33	36.749492666318375	39.0	35.0	40.0	31.0	41.0
34	36.79142810433126	39.0	35.0	41.0	31.0	41.0
35	36.661666186876495	39.0	35.0	40.0	30.0	41.0
36	36.631213726974515	39.0	35.0	40.0	30.0	41.0
37	36.559669904361826	39.0	35.0	40.0	30.0	41.0
38	36.41888525196092	39.0	35.0	40.0	30.0	41.0
39	36.2975374325184	39.0	35.0	40.0	30.0	41.0
40	36.1095201032082	38.0	35.0	40.0	30.0	41.0
41	36.11369072370128	38.0	35.0	40.0	30.0	41.0
42	36.12836519495474	38.0	35.0	40.0	30.0	41.0
43	36.05257256382098	38.0	35.0	40.0	30.0	41.0
44	36.00870552611658	38.0	35.0	40.0	30.0	41.0
45	35.766025880237265	38.0	35.0	40.0	29.0	41.0
46	35.538436523760275	38.0	34.0	40.0	28.0	41.0
47	35.43069519818888	38.0	34.0	40.0	28.0	41.0
48	35.425275346248576	38.0	34.0	40.0	28.0	41.0
49	35.290114900861134	38.0	34.0	40.0	27.0	41.0
50	35.202275982414804	37.0	34.0	40.0	27.0	41.0
51	33.79280527982429	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	4.0
11	5.0
12	2.0
13	12.0
14	7.0
15	27.0
16	36.0
17	90.0
18	190.0
19	368.0
20	656.0
21	1074.0
22	1550.0
23	2060.0
24	2597.0
25	3164.0
26	3992.0
27	4950.0
28	6454.0
29	8373.0
30	11205.0
31	14434.0
32	19013.0
33	25617.0
34	39259.0
35	46727.0
36	54585.0
37	76684.0
38	114115.0
39	125460.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.02261411009585	34.196067142192035	21.16461067693062	25.616708070781492
2	23.223443613992814	34.12587561706347	19.05477782161046	23.595902947333254
3	20.321601575133364	28.599048238459268	23.72082609205574	27.358524094351626
4	23.28261773517715	29.76191745476645	18.642691374083512	28.312773435972886
5	17.838598586218293	36.15112324210212	17.814964477757282	28.1953136939223
6	20.1819293251307	34.688118618346465	21.718146375096403	23.411805681426433
7	78.47998919583613	8.444129322998299	7.716092162360994	5.359789318804576
8	79.94494852029158	4.853166437433585	5.746820057361581	9.455064984913264
9	75.06637097376081	7.822001400276502	10.660582216488432	6.451045409474257
10	34.464394238253135	32.36504568668636	14.823028506644206	18.3475315684163
11	26.895615428630325	27.28886566941391	25.957181392670936	19.85833750928483
12	25.597161063783663	22.370305608569407	32.92586708746042	19.106666240186517
13	26.572912113102536	25.68458949508304	25.730969211686975	22.011529180127447
14	19.645452833072113	26.992461963301384	31.872105710213845	21.48997949341266
15	18.266678039470737	29.32210979731531	29.075462109015437	23.33575005419852
16	20.595437373166583	27.460168530740336	28.546626719692362	23.397767376400722
17	19.816222594207687	27.653683900018837	28.014592729224198	24.515500776549278
18	22.485099849665747	26.002494908893176	26.107871046617838	25.404534194823242
19	21.87914263273306	30.13704228906114	27.884693982720442	20.099121095485355
20	23.67195857456117	29.491457957941947	26.059003529123263	20.77757993837362
21	22.63365710284924	27.724053125211018	26.057404228550713	23.584885543389024
22	20.655144594541767	28.18607329061424	26.85172351291702	24.30705860192698
23	21.617923539216626	26.84177230935449	25.34376077306636	26.19654337836253
24	19.689700148912653	29.934108816410955	26.02986071869014	24.346330315986254
25	21.94347005576228	27.787314347858537	25.75566952052969	24.513546075849497
26	23.11842287639539	27.02071627341643	23.83988513467888	26.020975715509305
27	19.066506025809158	26.82258070248389	27.61725538697743	26.493657884729522
28	20.847060663247717	25.594673262893032	28.819751717471114	24.738514356388137
29	20.857189566873867	28.799138510091588	24.19564066203936	26.148031260995193
30	22.098957611426826	27.840624366943523	25.650293382805028	24.41012463882462
31	22.13751852523163	28.350445849459614	22.932015509661554	26.5800201156472
32	21.27247461554591	26.656075742875117	23.117001275886455	28.954448365692514
33	20.59597047335743	28.679368667213982	25.03331876192812	25.69134209750047
34	18.354284170833736	27.165541825263972	25.569972954050318	28.910201049851974
35	18.86534955379514	27.245151453764223	26.416891457247143	27.4726075351935
36	20.31076187125275	29.56626968472455	25.411642197367907	24.711326246654796
37	19.186631268813994	30.629093765215572	20.610541878573994	29.573733087396448
38	21.402373362049666	28.26568291911449	24.631005817900085	25.700937900935767
39	19.93723633753061	26.633863234923037	25.461931315371412	27.966969112174944
40	21.622543740870658	28.038937637939675	24.04921580961926	26.28930281157041
41	19.226080682936885	26.07268643402174	28.187494891123173	26.5137379919182
42	22.166305935537526	25.965888695788152	24.584626101296145	27.283179267378177
43	20.291747964445772	24.588890902822943	27.13035721266788	27.989003920063404
44	21.61046013654473	22.460044140695803	26.107871046617838	29.821624676141635
45	24.58373760097806	24.077292419670687	25.63661047790655	25.7023595014447
46	18.901422666709315	24.805507280371604	30.191773908655062	26.10129614426402
47	20.43390801533907	27.263632260380348	24.605594708802904	27.696865015477673
48	19.241362888407913	24.713636347481813	30.172049201593616	25.87295156251666
49	21.025649227182424	23.91256446069808	28.415128672616063	26.646657639503434
50	20.46322852583581	23.041478748849393	26.747235875510444	29.74805684980435
51	19.904717225888767	21.462791383679317	31.136249746777413	27.496241643654507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1823.0
1	1464.5
2	1106.0
3	757.5
4	409.0
5	387.5
6	366.0
7	396.5
8	427.0
9	455.5
10	484.0
11	512.5
12	541.0
13	629.0
14	717.0
15	778.0
16	839.0
17	993.0
18	1147.0
19	1033.5
20	920.0
21	1131.5
22	1343.0
23	2145.5
24	2948.0
25	2919.5
26	3747.0
27	4603.0
28	5412.5
29	6222.0
30	7678.0
31	9134.0
32	9861.0
33	10588.0
34	12181.5
35	13775.0
36	13954.0
37	14133.0
38	14995.5
39	15858.0
40	18256.0
41	20654.0
42	23699.0
43	26744.0
44	38624.5
45	50505.0
46	52161.0
47	53817.0
48	57165.0
49	60513.0
50	56339.0
51	52165.0
52	47259.5
53	42354.0
54	38133.5
55	33913.0
56	30497.0
57	27081.0
58	25208.5
59	23336.0
60	22284.5
61	21233.0
62	19662.0
63	18091.0
64	15665.5
65	13240.0
66	11484.5
67	9729.0
68	8078.5
69	6428.0
70	5479.0
71	4530.0
72	3831.5
73	3133.0
74	2789.5
75	1925.0
76	1404.0
77	1034.5
78	665.0
79	466.5
80	268.0
81	182.5
82	97.0
83	69.0
84	41.0
85	42.0
86	43.0
87	30.5
88	18.0
89	15.5
90	13.0
91	9.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.99603675995878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58895311691684	20.406021569910877
2	10.306943094209426	5.564932293081619
3	3.7268038382803925	3.018268002461188
4	1.7949267648138174	1.9382363569739078
5	1.0381765686089073	1.4013326404746966
6	0.6605370980865425	1.0699130268756478
7	0.5043366272753329	0.9530563090522961
8	0.37494446612276944	0.8097611672314722
9	0.32566141812577354	0.791241085352132
>10	4.224745018560337	28.134190524160264
>50	1.2948505674950979	23.898836225077975
>100	0.148513326737789	6.291334092084499
>500	0.0053040473834924644	1.1310082539937967
>1k	0.0033150296146827905	1.692664196553147
>5k	0.001989017768809674	2.899204256716493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	5734	1.018932164777715	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	5396	0.9588695432752965	No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	5068	0.9005839224090442	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	4551	0.8087129895192502	RNA PCR Primer, Index 16 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1427	0.2535779907809207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1387	0.2464699882362558	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	1067	0.1896059678789365	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1025	0.18214256520703834	RNA PCR Primer, Index 14 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	989	0.17574536291683993	RNA PCR Primer, Index 16 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	831	0.14766875286541353	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	819	0.14553635210201404	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	815	0.14482555184754756	RNA PCR Primer, Index 16 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACTC	771	0.13700674904841614	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	735	0.13060954675821773	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	686	0.12190224364100322	No Hit
TGCCACGGGAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTC	673	0.11959214281398711	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7770006361662278E-4	0.0	0.0	0.9714862477920767	0.0
2	1.7770006361662278E-4	0.0	0.0	1.3508758836135664	0.0
3	1.7770006361662278E-4	0.0	0.0	1.9186275868686762	0.0
4	1.7770006361662278E-4	0.0	0.0	4.6992781823415894	0.0
5	1.7770006361662278E-4	0.0	0.0	5.03068880098659	0.0
6	1.7770006361662278E-4	0.0	0.0	6.646870879579775	0.0
7	1.7770006361662278E-4	0.0	0.0	7.850788810582394	0.0
8	1.7770006361662278E-4	0.0	0.0	8.61863078546982	0.0
9	1.7770006361662278E-4	0.0	0.0	10.82228927437956	0.0
10	1.7770006361662278E-4	0.0	0.0	12.486094970022	0.0
11	1.7770006361662278E-4	0.0	0.0	14.9790491624996	0.0
12	1.7770006361662278E-4	0.0	0.0	15.801800457044564	0.0
13	1.7770006361662278E-4	0.0	0.0	16.19753849871878	0.0
14	1.7770006361662278E-4	0.0	0.0	16.731882590013967	0.0
15	1.7770006361662278E-4	0.0	0.0	17.094035319664645	0.0
16	1.7770006361662278E-4	0.0	0.0	17.78777636802394	0.0
17	1.7770006361662278E-4	0.0	0.0	18.65903977993624	0.0
18	1.7770006361662278E-4	0.0	0.0	19.984149154325397	0.0
19	1.7770006361662278E-4	0.0	0.0	20.441904518201817	0.0
20	1.7770006361662278E-4	0.0	0.0	21.039154432017288	0.0
21	1.7770006361662278E-4	0.0	0.0	21.594822530946466	0.0
22	1.7770006361662278E-4	0.0	0.0	22.171103837255174	0.0
23	1.7770006361662278E-4	0.0	0.0	22.758935647698962	0.0
24	1.7770006361662278E-4	0.0	0.0	23.179551698279507	0.0
25	1.7770006361662278E-4	0.0	0.0	23.53832812672147	0.0
26	1.7770006361662278E-4	0.0	0.0	23.84663773709631	0.0
27	1.7770006361662278E-4	0.0	0.0	24.26600988723154	0.0
28	1.7770006361662278E-4	0.0	0.0	24.654639926361092	0.0
29	1.7770006361662278E-4	0.0	0.0	25.021590557729418	0.0
30	1.7770006361662278E-4	0.0	0.0	25.5836558589488	0.0
31	1.7770006361662278E-4	0.0	0.0	25.920042079375065	0.0
32	1.7770006361662278E-4	0.0	0.0	26.28024010832596	0.0
33	1.7770006361662278E-4	0.0	0.0	26.63119773396879	0.0
34	1.7770006361662278E-4	0.0	0.0	26.97522505713057	0.0
35	1.7770006361662278E-4	0.0	0.0	27.349105990979943	0.0
36	1.7770006361662278E-4	0.0	0.0	27.68016120949771	0.0
37	1.7770006361662278E-4	0.0	0.0	28.037338337367125	0.0
38	1.7770006361662278E-4	0.0	0.0	28.374613058111475	0.0
39	1.7770006361662278E-4	0.0	0.0	28.749737892406166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTA	20	7.030212E-4	45.000004	44
CGGGTAT	100	0.0	45.000004	6
AGCCGTA	20	7.030212E-4	45.000004	32
GTCGCTC	25	3.888258E-5	45.000004	9
AATCCGA	25	3.888258E-5	45.000004	24
ATCTCGC	20	7.030212E-4	45.000004	23
TACCACG	20	7.030212E-4	45.000004	1
AGATACG	25	3.888258E-5	45.000004	1
CGAAACG	25	3.888258E-5	45.000004	1
GCGCGAC	60	0.0	45.000004	9
TTTCGCA	30	2.1635187E-6	45.000004	14
CATTGCG	20	7.030212E-4	45.000004	1
CGACCAT	20	7.030212E-4	45.000004	12
ATTGTCG	25	3.888258E-5	45.000004	41
TTCGTCC	20	7.030212E-4	45.000004	36
ATTCGCC	20	7.030212E-4	45.000004	23
CGCGACC	60	0.0	45.000004	10
CGTTCCA	20	7.030212E-4	45.000004	36
GCACCTT	20	7.030212E-4	45.000004	24
ACCTATC	20	7.030212E-4	45.000004	27
>>END_MODULE