SRR3553199_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447286
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	3057	0.6834553283581423	Illumina PCR Primer Index 5 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGT	2789	0.6235384071936076	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC	2600	0.5812835635365292	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	2071	0.4630147154169815	Illumina PCR Primer Index 5 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1132	0.25308192073975044	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	813	0.18176289890584546	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	633	0.14152019066100885	TruSeq Adapter, Index 23 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	583	0.13034166059299868	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	566	0.12654096036987522	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.10619603564609668	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTA	60	0.0	45.000004	42
AAGCCGT	30	2.1625165E-6	45.000004	26
ACCGTAG	30	2.1625165E-6	45.000004	26
ACGTACG	30	2.1625165E-6	45.000004	1
CGATATG	30	2.1625165E-6	45.000004	1
ACAACGT	20	7.02866E-4	45.0	18
AACGTAG	20	7.02866E-4	45.0	1
TAATACG	20	7.02866E-4	45.0	1
CGGAACA	20	7.02866E-4	45.0	37
TCGCATT	20	7.02866E-4	45.0	22
AAATCCG	45	3.8380676E-10	45.0	1
CGAACGT	20	7.02866E-4	45.0	39
GTCGATT	20	7.02866E-4	45.0	28
CTCGTGC	40	6.7993824E-9	45.0	44
TCCGCAA	25	3.8869708E-5	45.0	10
ATTAGCG	55	1.8189894E-12	45.0	1
CGAAATC	25	3.8869708E-5	45.0	31
TCCGAAC	20	7.02866E-4	45.0	37
CGCATTT	20	7.02866E-4	45.0	23
GACGTCT	20	7.02866E-4	45.0	21