##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447286
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854305746211597	33.0	31.0	34.0	30.0	34.0
2	32.06557102167294	33.0	31.0	34.0	30.0	34.0
3	32.2038785027924	34.0	31.0	34.0	30.0	34.0
4	35.858191403263234	37.0	35.0	37.0	35.0	37.0
5	35.63129854276682	37.0	35.0	37.0	33.0	37.0
6	35.66273480502408	37.0	35.0	37.0	33.0	37.0
7	35.73057953971285	37.0	35.0	37.0	35.0	37.0
8	35.784012913437934	37.0	35.0	37.0	35.0	37.0
9	37.52043211725831	39.0	37.0	39.0	35.0	39.0
10	37.06735958648382	39.0	37.0	39.0	33.0	39.0
11	37.03123952012806	39.0	37.0	39.0	33.0	39.0
12	36.95665860322031	39.0	37.0	39.0	33.0	39.0
13	36.94044079179764	39.0	37.0	39.0	33.0	39.0
14	38.11934869412412	40.0	37.0	41.0	33.0	41.0
15	38.16570829402217	40.0	37.0	41.0	33.0	41.0
16	38.05742634466538	40.0	37.0	41.0	33.0	41.0
17	38.026014675174274	40.0	37.0	41.0	33.0	41.0
18	37.964409795969466	40.0	37.0	41.0	33.0	41.0
19	37.99841712014237	40.0	37.0	41.0	33.0	41.0
20	37.90345327150861	40.0	37.0	41.0	33.0	41.0
21	37.904834937825015	40.0	37.0	41.0	33.0	41.0
22	37.87404032319366	40.0	37.0	41.0	33.0	41.0
23	37.73571719213211	40.0	37.0	41.0	33.0	41.0
24	37.6207102390864	39.0	36.0	41.0	32.0	41.0
25	37.68492642291509	40.0	36.0	41.0	33.0	41.0
26	37.63144162795169	40.0	36.0	41.0	33.0	41.0
27	37.608324427771045	40.0	36.0	41.0	33.0	41.0
28	37.543287739835364	40.0	36.0	41.0	33.0	41.0
29	37.40290105212325	39.0	36.0	41.0	32.0	41.0
30	37.453038995184286	39.0	36.0	41.0	32.0	41.0
31	37.39473401805556	39.0	36.0	41.0	32.0	41.0
32	37.34321664438413	39.0	36.0	41.0	32.0	41.0
33	37.27346038105373	39.0	36.0	41.0	32.0	41.0
34	37.23495481638147	39.0	36.0	41.0	32.0	41.0
35	37.16352624495289	39.0	36.0	41.0	32.0	41.0
36	37.0984873213112	39.0	35.0	41.0	31.0	41.0
37	37.040846348868506	39.0	35.0	41.0	31.0	41.0
38	36.97176526875422	39.0	35.0	41.0	31.0	41.0
39	36.90154174286698	39.0	35.0	41.0	31.0	41.0
40	36.73460381053733	39.0	35.0	41.0	31.0	41.0
41	36.68186350567646	39.0	35.0	40.0	31.0	41.0
42	36.638434916362236	39.0	35.0	40.0	31.0	41.0
43	36.55513251029543	39.0	35.0	40.0	31.0	41.0
44	36.49203194376752	39.0	35.0	40.0	31.0	41.0
45	36.31525690497802	38.0	35.0	40.0	30.0	41.0
46	36.143758132380626	38.0	35.0	40.0	30.0	41.0
47	35.98559534615436	38.0	35.0	40.0	30.0	41.0
48	35.94877550381635	38.0	35.0	40.0	30.0	41.0
49	35.77107488273722	38.0	35.0	40.0	29.0	41.0
50	35.66729564529183	38.0	34.0	40.0	29.0	41.0
51	34.37256028581265	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	4.0
13	8.0
14	12.0
15	21.0
16	44.0
17	66.0
18	172.0
19	283.0
20	478.0
21	766.0
22	1052.0
23	1388.0
24	1824.0
25	2157.0
26	2682.0
27	3236.0
28	4226.0
29	5401.0
30	7171.0
31	9717.0
32	12807.0
33	17395.0
34	26886.0
35	34647.0
36	40990.0
37	60764.0
38	96325.0
39	116736.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.460287154080387	34.104130243289525	20.289702785242554	25.145879817387534
2	23.833296816801777	31.28401067773192	20.720970475266384	24.16172203019992
3	20.393215973672326	30.984649642510607	22.708513121358596	25.91362126245847
4	22.640994799747812	30.55069910527045	19.450865888939067	27.357440206042664
5	17.87066887852515	35.23025536234087	18.128669352494825	28.770406406639154
6	20.492481320676255	35.504800060811206	21.784048684734152	22.218669933778386
7	83.06251481155233	6.723662265306761	5.748447302173553	4.465375620967346
8	84.17924996534656	4.390479469511678	5.018936429935209	6.411334135206557
9	78.6161426917006	8.112035699753624	8.37093045612874	4.900891152417022
10	35.263567381943545	34.410869108355726	13.31295859919604	17.012604910504688
11	26.095160590762955	28.623744092146858	25.586761043269856	19.69433427382033
12	26.472771336460344	23.915347227500973	30.267211582745716	19.34466985329297
13	26.61205582110775	26.13070831637923	26.176093148455347	21.08114271405767
14	20.828284363919284	27.15466167060896	29.586215531002537	22.430838434469223
15	19.613178145526575	28.892699525583183	27.893115366901718	23.601006961988528
16	21.41784003970614	27.69033683146801	27.8314098809263	23.060413247899554
17	20.73617327615888	28.2313776867597	27.91949669786222	23.112952339219202
18	23.57373134862258	26.07235638942422	26.250095017505576	24.103817244447622
19	22.204808556494054	29.892060113663295	26.599535867431577	21.303595462411074
20	24.7354041932902	28.772865683254118	25.86667143617283	20.625058687282856
21	23.806691915239913	27.401483614510624	27.305571826527096	21.486252643722363
22	21.574563031259643	27.167628765487855	26.628376475007045	24.629431728245464
23	22.777372866577537	27.614099256404177	24.92230921602733	24.686218660990956
24	20.24364724136235	29.667595229897646	26.806338673689766	23.282418855050235
25	22.525632369445947	27.631984904512997	25.581395348837212	24.260987377203847
26	24.485675831570852	28.464338253377036	24.3191157335575	22.730870181494613
27	21.304266174215154	25.655620788488797	27.47257906574317	25.567533971552876
28	23.117870892449126	26.813492932933293	27.363476612279392	22.70515956233819
29	24.25271526495352	28.53521013400822	24.117231480529234	23.094843120509026
30	23.50710730941724	25.666799318556805	26.271557795236156	24.554535576789792
31	25.10608425034542	27.074399824720647	24.457953077002188	23.36156284793175
32	24.35376917676833	25.612918803628997	24.46264805963075	25.570663959971917
33	22.337162352499295	25.183439678416047	28.69059170195356	23.788806267131097
34	22.437545552510027	25.432273757729956	28.63626404582303	23.493916643936988
35	22.924705892873913	23.397334144149383	28.509052373649073	25.16890758932763
36	23.173539972187818	25.862647165348346	29.445589622746965	21.51822323971687
37	22.528985928466348	26.553927464754096	25.602410985365072	25.314675621414484
38	22.89295886748076	25.612695233027637	29.37270560670354	22.121640292788058
39	23.689988061329885	22.841984770370637	28.368650035994868	25.09937713230461
40	23.995832643990646	24.926557057453174	27.931793080937027	23.145817217619154
41	20.553516094847595	23.37318851920248	31.178485353889908	24.894810032060025
42	24.433136740251204	24.18676193755226	26.6078079796819	24.772293342514633
43	22.62936912847708	23.953354229732206	28.14060802260746	25.27666861918325
44	23.03470262874313	22.183569349364838	27.947443023032243	26.834284998859793
45	25.75756898270905	23.39800485595346	27.412662144578636	23.43176401675885
46	21.735086723036268	23.950671382515885	30.809370291044207	23.504871603403636
47	22.554249406420055	25.241568034769703	27.82492633348685	24.379256225323395
48	21.633585670018736	23.486762384693463	31.34884615212638	23.53080579316142
49	22.570346489717988	22.58241930219144	29.026841886399307	25.820392321691266
50	21.982802949343373	23.56143496554777	27.360123053258988	27.095639031849867
51	22.05948766560992	22.037130605473905	30.900587096399175	25.002794632517002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1431.0
1	1152.0
2	873.0
3	621.5
4	370.0
5	360.5
6	351.0
7	370.5
8	390.0
9	436.5
10	483.0
11	525.5
12	568.0
13	579.5
14	591.0
15	608.5
16	626.0
17	700.0
18	774.0
19	998.0
20	1222.0
21	1304.0
22	1386.0
23	1876.5
24	2367.0
25	2691.5
26	3567.5
27	4119.0
28	4910.5
29	5702.0
30	6466.5
31	7231.0
32	7818.0
33	8405.0
34	9131.5
35	9858.0
36	11321.0
37	12784.0
38	14244.0
39	15704.0
40	16304.0
41	16904.0
42	19845.0
43	22786.0
44	28769.0
45	34752.0
46	36373.0
47	37994.0
48	41605.0
49	45216.0
50	42371.0
51	39526.0
52	36512.5
53	33499.0
54	29103.5
55	24708.0
56	22975.5
57	21243.0
58	21503.5
59	21764.0
60	20172.0
61	18580.0
62	16570.5
63	14561.0
64	13026.5
65	11492.0
66	9699.0
67	7906.0
68	7110.0
69	6314.0
70	5587.0
71	4860.0
72	3583.5
73	2307.0
74	2228.0
75	1737.5
76	1326.0
77	1004.5
78	683.0
79	452.5
80	222.0
81	181.0
82	140.0
83	99.0
84	58.0
85	37.5
86	17.0
87	15.5
88	14.0
89	10.0
90	6.0
91	3.0
92	0.0
93	3.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.79236577214854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92915081257198	19.583924305264862
2	10.112723442600611	5.216621239682723
3	3.511577952781767	2.7171570898667965
4	1.619645770644899	1.6709798455114655
5	0.934247379666234	1.2048225069011418
6	0.6172513453461644	0.955222348351143
7	0.4997122358369361	0.9022132537267087
8	0.37783008450761435	0.7796105391473747
9	0.28467828743648516	0.6608273865255597
>10	4.318926136185185	28.704785026158213
>50	1.6244942876369037	28.52582498094623
>100	0.16192708333358205	5.879634187075544
>500	0.0034823028673888617	0.582686679987055
>1k	0.004352878584236077	2.6156906108551845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	3057	0.6834553283581423	Illumina PCR Primer Index 5 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGT	2789	0.6235384071936076	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC	2600	0.5812835635365292	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	2071	0.4630147154169815	Illumina PCR Primer Index 5 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1132	0.25308192073975044	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	813	0.18176289890584546	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	633	0.14152019066100885	TruSeq Adapter, Index 23 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	583	0.13034166059299868	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	566	0.12654096036987522	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.10619603564609668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2357060136020353E-4	0.0	0.0	0.7538800677866063	0.0
2	2.2357060136020353E-4	0.0	0.0	1.0601717916500852	0.0
3	2.2357060136020353E-4	0.0	0.0	1.4625988740984515	0.0
4	2.2357060136020353E-4	0.0	0.0	3.298560652468443	0.0
5	2.2357060136020353E-4	0.0	0.0	3.526826236457211	0.0
6	2.2357060136020353E-4	0.0	0.0	4.75624097333697	0.0
7	2.2357060136020353E-4	0.0	0.0	5.608715676323426	0.0
8	2.2357060136020353E-4	0.0	0.0	6.224429112469426	0.0
9	2.2357060136020353E-4	0.0	0.0	7.647455990127122	0.0
10	2.2357060136020353E-4	0.0	0.0	8.927174112312928	0.0
11	2.2357060136020353E-4	0.0	0.0	10.706572528538787	0.0
12	2.2357060136020353E-4	0.0	0.0	11.33547663016504	0.0
13	2.2357060136020353E-4	0.0	0.0	11.616504876074815	0.0
14	2.2357060136020353E-4	0.0	0.0	12.00641200484701	0.0
15	2.2357060136020353E-4	0.0	0.0	12.282745268128222	0.0
16	2.2357060136020353E-4	0.0	0.0	12.828033964845758	0.0
17	2.2357060136020353E-4	0.0	0.0	13.503664322156293	0.0
18	2.2357060136020353E-4	0.0	0.0	14.493411374377915	0.0
19	2.2357060136020353E-4	0.0	0.0	14.896062027427641	0.0
20	2.2357060136020353E-4	0.0	0.0	15.30184266889641	0.0
21	2.2357060136020353E-4	0.0	0.0	15.731992505913443	0.0
22	2.2357060136020353E-4	0.0	0.0	16.184946544269216	0.0
23	2.2357060136020353E-4	0.0	0.0	16.654221236524283	0.0
24	2.2357060136020353E-4	0.0	0.0	16.999414245024436	0.0
25	2.2357060136020353E-4	0.0	0.0	17.29788099784031	0.0
26	2.2357060136020353E-4	0.0	0.0	17.584051367581367	0.0
27	2.2357060136020353E-4	0.0	0.0	17.916053710601272	0.0
28	2.2357060136020353E-4	0.0	0.0	18.241572506181726	0.0
29	2.2357060136020353E-4	0.0	0.0	18.56261988973498	0.0
30	2.2357060136020353E-4	0.0	0.0	19.01222036907035	0.0
31	2.2357060136020353E-4	0.0	0.0	19.315605675116146	0.0
32	2.2357060136020353E-4	0.0	0.0	19.63732377047348	0.0
33	2.2357060136020353E-4	0.0	0.0	19.94763976516144	0.0
34	2.2357060136020353E-4	0.0	0.0	20.236269411517462	0.0
35	2.2357060136020353E-4	0.0	0.0	20.568495325138727	0.0
36	2.2357060136020353E-4	0.0	0.0	20.854218553677065	0.0
37	2.2357060136020353E-4	0.0	0.0	21.14217748822901	0.0
38	2.2357060136020353E-4	0.0	0.0	21.446233506078883	0.0
39	2.2357060136020353E-4	0.0	0.0	21.7708580192539	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTA	60	0.0	45.000004	42
AAGCCGT	30	2.1625165E-6	45.000004	26
ACCGTAG	30	2.1625165E-6	45.000004	26
ACGTACG	30	2.1625165E-6	45.000004	1
CGATATG	30	2.1625165E-6	45.000004	1
ACAACGT	20	7.02866E-4	45.0	18
AACGTAG	20	7.02866E-4	45.0	1
TAATACG	20	7.02866E-4	45.0	1
CGGAACA	20	7.02866E-4	45.0	37
TCGCATT	20	7.02866E-4	45.0	22
AAATCCG	45	3.8380676E-10	45.0	1
CGAACGT	20	7.02866E-4	45.0	39
GTCGATT	20	7.02866E-4	45.0	28
CTCGTGC	40	6.7993824E-9	45.0	44
TCCGCAA	25	3.8869708E-5	45.0	10
ATTAGCG	55	1.8189894E-12	45.0	1
CGAAATC	25	3.8869708E-5	45.0	31
TCCGAAC	20	7.02866E-4	45.0	37
CGCATTT	20	7.02866E-4	45.0	23
GACGTCT	20	7.02866E-4	45.0	21
>>END_MODULE