FastQCFastQC Report
Sat 18 Jun 2016
SRR3553197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354878
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT23270.6557183031915192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12370.34857049464886525No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT6610.186261193987793No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6360.17921651947993394No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT4540.12793128906272014No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC4310.12145018851548983No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT4240.1194776796532893No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC4150.11694159683046004No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT4150.11694159683046004No Hit
ATGGGAGGGCATGCAAAAACTAACATTGAAATTTTGAAACAGCAGCAGAGT3640.10257046083442761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA900.045.00000424
CGTTGAT900.045.00000425
CTTAACA253.8853374E-545.021
TATCAGG406.7921064E-945.02
GAGGATA207.02669E-445.020
TAGAGTC253.8853374E-545.019
AACGTAG207.02669E-445.01
AGCGTGC207.02669E-445.015
AGGTACC253.8853374E-545.012
TCGCATC253.8853374E-545.041
GGTACGT207.02669E-445.031
GTCGCAC207.02669E-445.034
TCGCACG207.02669E-445.035
CGTATAG207.02669E-445.01
CAACGGT207.02669E-445.030
CGACGGT207.02669E-445.028
ACGTTAA351.2087548E-745.010
ACTTGCG351.2087548E-745.01
TCACACG351.2087548E-745.01
ACCGCGT207.02669E-445.025