##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354878
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847649051223236	33.0	31.0	34.0	30.0	34.0
2	32.046706191987106	33.0	31.0	34.0	30.0	34.0
3	32.19282401275932	34.0	31.0	34.0	30.0	34.0
4	35.85739888074212	37.0	35.0	37.0	35.0	37.0
5	35.64770146360158	37.0	35.0	37.0	33.0	37.0
6	35.66310393994556	37.0	35.0	37.0	33.0	37.0
7	35.73997824604512	37.0	35.0	37.0	35.0	37.0
8	35.76896849057986	37.0	35.0	37.0	35.0	37.0
9	37.46106267506016	39.0	37.0	39.0	35.0	39.0
10	37.040422342326096	39.0	37.0	39.0	33.0	39.0
11	37.037610108262555	39.0	37.0	39.0	33.0	39.0
12	36.95103951217038	39.0	37.0	39.0	33.0	39.0
13	36.90560417946449	39.0	37.0	39.0	33.0	39.0
14	38.054686399269606	40.0	37.0	41.0	33.0	41.0
15	38.07673341260941	40.0	37.0	41.0	33.0	41.0
16	38.01429223564154	40.0	37.0	41.0	33.0	41.0
17	37.9716747727388	40.0	37.0	41.0	33.0	41.0
18	37.871099363725	40.0	37.0	41.0	33.0	41.0
19	37.910946860611254	40.0	37.0	41.0	33.0	41.0
20	37.7989421716759	40.0	36.0	41.0	33.0	41.0
21	37.7648797614955	40.0	36.0	41.0	33.0	41.0
22	37.72851515168593	40.0	36.0	41.0	33.0	41.0
23	37.579666815074475	39.0	36.0	41.0	33.0	41.0
24	37.47451518550037	39.0	36.0	41.0	32.0	41.0
25	37.481751475154844	39.0	36.0	41.0	32.0	41.0
26	37.4545533958149	39.0	36.0	41.0	32.0	41.0
27	37.41309125953144	39.0	36.0	41.0	32.0	41.0
28	37.27148766618387	39.0	36.0	41.0	32.0	41.0
29	37.10963486043091	39.0	35.0	41.0	31.0	41.0
30	37.173668133837545	39.0	35.0	41.0	32.0	41.0
31	37.04342619153625	39.0	35.0	41.0	31.0	41.0
32	37.0510936152706	39.0	35.0	41.0	31.0	41.0
33	36.98891731806424	39.0	35.0	41.0	31.0	41.0
34	36.854462660407236	39.0	35.0	41.0	31.0	41.0
35	36.82255873849605	39.0	35.0	41.0	31.0	41.0
36	36.73055810729321	39.0	35.0	41.0	31.0	41.0
37	36.64935837104582	39.0	35.0	41.0	31.0	41.0
38	36.58816269253096	39.0	35.0	41.0	30.0	41.0
39	36.537193063531696	39.0	35.0	40.0	30.0	41.0
40	36.39809173856931	39.0	35.0	40.0	30.0	41.0
41	36.32460451197313	39.0	35.0	40.0	30.0	41.0
42	36.26388787132479	38.0	35.0	40.0	30.0	41.0
43	36.190986761647665	38.0	35.0	40.0	30.0	41.0
44	36.12258579004616	38.0	35.0	40.0	30.0	41.0
45	35.90161407582324	38.0	35.0	40.0	30.0	41.0
46	35.763842785407945	38.0	35.0	40.0	29.0	41.0
47	35.59240922232429	38.0	34.0	40.0	29.0	41.0
48	35.52128055275334	38.0	34.0	40.0	28.0	41.0
49	35.36857737025119	37.0	34.0	40.0	28.0	41.0
50	35.27308258049245	37.0	34.0	40.0	28.0	41.0
51	34.01061773341824	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	4.0
13	3.0
14	9.0
15	14.0
16	31.0
17	69.0
18	137.0
19	273.0
20	460.0
21	821.0
22	1106.0
23	1410.0
24	1780.0
25	2092.0
26	2461.0
27	2932.0
28	3684.0
29	4774.0
30	6214.0
31	8001.0
32	10724.0
33	14438.0
34	23331.0
35	29107.0
36	32672.0
37	47708.0
38	74462.0
39	86130.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.83983791612892	31.79430677584973	20.66203033155056	26.703824976470784
2	24.04488303022447	30.299426845282042	20.519446119511496	25.136244004981993
3	20.963824187467242	31.05546131346547	20.063796572343172	27.916917926724118
4	21.0669582222623	31.167330744650272	18.73545274714127	29.030258285946157
5	18.769267184778997	33.811337980939925	18.873528367495307	28.545866466785768
6	20.590174651570397	36.80955145148474	19.85950101161526	22.74077288532961
7	83.9226438381641	5.892729332333929	5.335636472252436	4.848990357249534
8	84.96891889607132	5.330000732646148	4.790942239304775	4.91013813197775
9	78.45315855026234	8.308207327588637	8.373300120041254	4.865334002107766
10	36.30430739578109	32.54047869972216	13.32204306832207	17.833170836174688
11	27.597935065008254	29.80235461200751	23.42072486882816	19.178985454156074
12	27.448869752421963	22.929006588179597	30.564024819797226	19.058098839601215
13	27.349962522331616	23.433405282942307	26.74412051465574	22.472511680070333
14	19.899796549800215	28.36185956864049	27.926216897074486	23.81212698448481
15	19.670421947824323	29.47829958464599	29.583124341322936	21.268154126206753
16	21.454978894155175	28.077536505503296	26.792587875269813	23.674896725071715
17	22.289631929846312	26.937989957112023	26.962787211379684	23.809590901661977
18	23.8276252684021	26.69678030196293	25.56737808486297	23.908216344772008
19	23.89609950461849	30.665468132710394	23.911316001555463	21.52711636111565
20	25.097639188678926	27.544395538748528	24.49320611590462	22.864759156667922
21	24.48334357159362	28.493454088447294	24.508422612841596	22.51477972711749
22	23.234463674840367	26.33102080151489	24.83360478812437	25.600910735520376
23	23.25644305930489	29.122402628508954	23.56612695067037	24.055027361515787
24	21.409892977304875	28.308038255400447	25.412395245690067	24.869673521604607
25	22.18677968203157	29.198203326213516	24.689329854203415	23.925687137551495
26	25.14779727117488	27.52974261577218	22.819109665857	24.50335044719594
27	21.068085370183557	26.539543166947517	25.451000061993135	26.941371400875795
28	21.803831175784357	27.651474591267984	27.55087663929576	22.9938175936519
29	23.614312524304125	29.510141513421512	22.747817559837465	24.127728402436894
30	23.5077970457453	27.048732240375568	24.89954294151793	24.543927772361208
31	23.89215448689409	29.919014421857653	21.41327442106865	24.77555667017961
32	24.609865925754768	25.264175294044716	25.16245019415123	24.96350858604929
33	24.1984569344958	27.416464249685806	24.102085787228287	24.28299302859011
34	21.910628441323496	26.828374821769735	26.481776835983073	24.7792199009237
35	24.65523362958538	24.368656270605673	27.599062212929514	23.377047886879435
36	24.539982754636807	28.590670596655755	24.381054897739503	22.48829175096794
37	22.631721323947946	30.316334064100904	22.34514396496824	24.706800646982906
38	23.73717164772119	27.882539915125758	24.86403778199832	23.51625065515473
39	24.137027372787266	25.311515506737525	24.738360788778117	25.81309633169709
40	24.29877309948771	26.024154779952546	25.999921099645512	23.67715102091423
41	21.832855234756735	24.242697490405156	28.73466374359639	25.189783531241723
42	23.651790192685937	25.574986333331456	26.284807736743332	24.48841573723928
43	23.045666398029745	24.800635711427592	26.14645032940898	26.007247561133685
44	24.279611584826334	23.784230073433687	24.906305829045476	27.029852512694504
45	25.56568736298108	24.757240516459177	26.03993485085015	23.63713726970959
46	21.196016659246276	25.57526812031177	28.450340680459203	24.778374539982757
47	22.72414745349106	26.892622253281413	26.977440134356033	23.4057901588715
48	23.13640180569097	24.605075547089424	28.276759900585553	23.981762746634054
49	22.791494541786193	24.889116823246297	27.8166017617322	24.50278687323531
50	21.315212551919252	24.88460823156127	26.598436645833218	27.201742570686267
51	22.338381077440697	22.943377724175633	28.870203281127598	25.84803791725607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1510.0
1	1211.0
2	912.0
3	628.5
4	345.0
5	315.5
6	286.0
7	309.0
8	332.0
9	345.0
10	358.0
11	430.0
12	502.0
13	541.5
14	581.0
15	704.5
16	828.0
17	947.0
18	1066.0
19	916.0
20	766.0
21	809.5
22	853.0
23	1221.5
24	1590.0
25	1668.5
26	2309.5
27	2872.0
28	3161.5
29	3451.0
30	3920.5
31	4390.0
32	5188.0
33	5986.0
34	7012.0
35	8038.0
36	9129.0
37	10220.0
38	10926.5
39	11633.0
40	11480.0
41	11327.0
42	13917.0
43	16507.0
44	19378.0
45	22249.0
46	24487.0
47	26725.0
48	29418.0
49	32111.0
50	32206.5
51	32302.0
52	30065.0
53	27828.0
54	25599.5
55	23371.0
56	22222.0
57	21073.0
58	20313.0
59	19553.0
60	18170.0
61	16787.0
62	15647.5
63	14508.0
64	12653.5
65	10799.0
66	8956.0
67	7113.0
68	6290.0
69	5467.0
70	3998.5
71	2530.0
72	2517.0
73	2504.0
74	2092.0
75	1247.0
76	814.0
77	750.0
78	686.0
79	570.5
80	455.0
81	271.0
82	87.0
83	64.5
84	42.0
85	57.5
86	73.0
87	43.5
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	354878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.402093113689773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98264160256673	21.09485513331342
2	9.73016330056353	5.332536815468978
3	3.3544486035128127	2.757567389356342
4	1.5651351240179343	1.7155191361538331
5	0.9069968327094731	1.2426805831863346
6	0.648883221586936	1.0668455074701728
7	0.4401299823125334	0.8442337930218271
8	0.384599563983382	0.8431066451005698
9	0.3002755954094854	0.7405361842661422
>10	4.044465468306528	27.741082851007953
>50	1.4211673727942085	26.942216761816738
>100	0.2169799679157583	8.30905268852958
>500	0.002056682160338941	0.36547771346772695
>1k	0.002056682160338941	1.0042887978403847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	2327	0.6557183031915192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.34857049464886525	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	661	0.186261193987793	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	636	0.17921651947993394	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	454	0.12793128906272014	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	431	0.12145018851548983	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT	424	0.1194776796532893	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	415	0.11694159683046004	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	415	0.11694159683046004	No Hit
ATGGGAGGGCATGCAAAAACTAACATTGAAATTTTGAAACAGCAGCAGAGT	364	0.10257046083442761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7332097227779688	0.0
2	0.0	0.0	0.0	1.017814572895474	0.0
3	0.0	0.0	0.0	1.3689211503671683	0.0
4	0.0	0.0	0.0	2.8508388798403956	0.0
5	0.0	0.0	0.0	3.078240972954086	0.0
6	0.0	0.0	0.0	4.227086491695737	0.0
7	0.0	0.0	0.0	5.098935408788372	0.0
8	0.0	0.0	0.0	5.814956125767165	0.0
9	0.0	0.0	0.0	6.810509527217804	0.0
10	0.0	0.0	0.0	8.41641352802935	0.0
11	0.0	0.0	0.0	9.70530717598724	0.0
12	0.0	0.0	0.0	10.370888023489762	0.0
13	0.0	0.0	0.0	10.65042070796161	0.0
14	0.0	0.0	0.0	10.94319738050823	0.0
15	0.0	0.0	0.0	11.255699141676859	0.0
16	0.0	0.0	0.0	11.813637362699295	0.0
17	0.0	0.0	0.0	12.4730188966349	0.0
18	0.0	0.0	0.0	13.524929694148412	0.0
19	0.0	0.0	0.0	13.895197786281482	0.0
20	0.0	0.0	0.0	14.426648031154368	0.0
21	0.0	0.0	0.0	14.866517507425087	0.0
22	0.0	0.0	0.0	15.33512925568787	0.0
23	0.0	0.0	0.0	15.805149938852225	0.0
24	0.0	0.0	0.0	16.140476445426316	0.0
25	0.0	0.0	0.0	16.432971330992622	0.0
26	0.0	0.0	0.0	16.674744560102344	0.0
27	0.0	0.0	0.0	17.029514368318125	0.0
28	0.0	0.0	0.0	17.328208567451348	0.0
29	0.0	0.0	0.0	17.660717204222298	0.0
30	0.0	0.0	0.0	18.098895958611127	0.0
31	0.0	0.0	0.0	18.396463009823094	0.0
32	0.0	0.0	0.0	18.721363398125554	0.0
33	0.0	0.0	0.0	19.018648662357204	0.0
34	0.0	0.0	0.0	19.30776210415974	0.0
35	0.0	0.0	0.0	19.624772457013396	0.0
36	0.0	0.0	0.0	19.9054322894065	0.0
37	0.0	0.0	0.0	20.19482751818935	0.0
38	0.0	0.0	0.0	20.504511409554834	0.0
39	0.0	0.0	0.0	20.855899774006843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	90	0.0	45.000004	24
CGTTGAT	90	0.0	45.000004	25
CTTAACA	25	3.8853374E-5	45.0	21
TATCAGG	40	6.7921064E-9	45.0	2
GAGGATA	20	7.02669E-4	45.0	20
TAGAGTC	25	3.8853374E-5	45.0	19
AACGTAG	20	7.02669E-4	45.0	1
AGCGTGC	20	7.02669E-4	45.0	15
AGGTACC	25	3.8853374E-5	45.0	12
TCGCATC	25	3.8853374E-5	45.0	41
GGTACGT	20	7.02669E-4	45.0	31
GTCGCAC	20	7.02669E-4	45.0	34
TCGCACG	20	7.02669E-4	45.0	35
CGTATAG	20	7.02669E-4	45.0	1
CAACGGT	20	7.02669E-4	45.0	30
CGACGGT	20	7.02669E-4	45.0	28
ACGTTAA	35	1.2087548E-7	45.0	10
ACTTGCG	35	1.2087548E-7	45.0	1
TCACACG	35	1.2087548E-7	45.0	1
ACCGCGT	20	7.02669E-4	45.0	25
>>END_MODULE