FastQCFastQC Report
Sat 18 Jun 2016
SRR3553196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133425
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT22170.19560182632287096Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCC18180.16039879127423518No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGT17930.1581930873238194No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT16590.14637051414959085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG600.045.0000041
TTACGCG207.033244E-445.0000041
TGCCGTA207.033244E-445.00000420
CGTATAG502.1827873E-1145.01
CGCAATA253.8907718E-545.036
TATACGG1000.042.752
ATATCCG800.042.1875041
TAAACCG700.041.7857171
TAGCCCG700.041.7857171
AACGGGC3000.040.54
TATTCCG501.0822987E-940.51
ATGCGAG501.0822987E-940.51
AATAGCG501.0822987E-940.51
TATACCG501.0822987E-940.51
AATACCG1450.040.3448261
CATACGA2850.040.26315718
TATCTCG451.9283107E-840.01
TATAGCG451.9283107E-840.01
AGGGCGT451.9283107E-840.06
TATAACG451.9283107E-840.01