FastQCFastQC Report
Sat 18 Jun 2016
SRR3553195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147632
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT23080.20110976340847936Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCC21090.18376971015098917No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGT20080.17496897960321775No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT19020.16573256932535865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG253.890801E-545.0000041
CCGATAG253.890801E-545.0000041
ACGACCG253.890801E-545.0000041
TATCTCG551.8189894E-1245.01
CGTAGCA207.0332823E-445.012
ACGTCTA453.8562575E-1045.043
GCCGTAT207.0332823E-445.042
CGTATAG302.1654996E-644.9999961
TCGTCGA302.1654996E-644.99999640
TTACGCG800.042.18751
TAATGCG700.041.7857171
GGCTACG700.041.7857171
ATATCCG1200.041.2499961
TAAACCG1100.040.9090921
ATCAGCG1600.040.781251
ATTAGCG501.0822987E-940.5000041
CTCGTAA501.0822987E-940.50000433
TATAGCG501.0822987E-940.5000041
TATACGG950.040.2631572
ATACGGG6200.039.9193533