##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147632
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.018600039036905	33.0	31.0	34.0	30.0	34.0
2	32.21878964685544	34.0	31.0	34.0	30.0	34.0
3	32.35636074978739	34.0	31.0	34.0	30.0	34.0
4	35.984713740990145	37.0	35.0	37.0	35.0	37.0
5	35.781435163885284	37.0	35.0	37.0	35.0	37.0
6	35.81054118393352	37.0	35.0	37.0	35.0	37.0
7	35.87625040082535	37.0	35.0	37.0	35.0	37.0
8	35.9073954020104	37.0	35.0	37.0	35.0	37.0
9	37.608234172626766	39.0	37.0	39.0	35.0	39.0
10	37.18620951663948	39.0	37.0	39.0	34.0	39.0
11	37.1749803072762	39.0	37.0	39.0	34.0	39.0
12	37.11138936523206	39.0	37.0	39.0	33.0	39.0
13	37.08583762042188	39.0	37.0	39.0	33.0	39.0
14	38.32550591130258	40.0	38.0	41.0	34.0	41.0
15	38.34921821629233	40.0	38.0	41.0	34.0	41.0
16	38.25417381181424	40.0	38.0	41.0	33.0	41.0
17	38.23402885245444	40.0	37.0	41.0	33.0	41.0
18	38.17627863287186	40.0	37.0	41.0	33.0	41.0
19	38.24566847212347	40.0	37.0	41.0	34.0	41.0
20	38.161924728484394	40.0	37.0	41.0	34.0	41.0
21	38.15248616281177	40.0	37.0	41.0	34.0	41.0
22	38.12250704058444	40.0	37.0	41.0	34.0	41.0
23	37.99606668339677	40.0	37.0	41.0	33.0	41.0
24	37.88672501289612	40.0	37.0	41.0	33.0	41.0
25	37.92861561894405	40.0	37.0	41.0	33.0	41.0
26	37.88707007124235	40.0	37.0	41.0	33.0	41.0
27	37.82205968463759	40.0	37.0	41.0	33.0	41.0
28	37.77851262425586	40.0	37.0	41.0	33.0	41.0
29	37.617535063504675	40.0	36.0	41.0	33.0	41.0
30	37.674496702775805	40.0	37.0	41.0	33.0	41.0
31	37.63930597961716	40.0	36.0	41.0	33.0	41.0
32	37.611521811870006	40.0	36.0	41.0	33.0	41.0
33	37.56416429656893	40.0	36.0	41.0	33.0	41.0
34	37.471870773906616	40.0	36.0	41.0	33.0	41.0
35	37.39911138762251	40.0	36.0	41.0	32.0	41.0
36	37.36430493398581	40.0	36.0	41.0	32.0	41.0
37	37.32571503757302	40.0	36.0	41.0	32.0	41.0
38	37.299552469781254	39.0	36.0	41.0	32.0	41.0
39	37.225526126842055	39.0	35.0	41.0	32.0	41.0
40	37.096016841635645	39.0	35.0	41.0	32.0	41.0
41	37.00766970596846	39.0	35.0	41.0	31.0	41.0
42	36.95522258006051	39.0	35.0	41.0	31.0	41.0
43	36.898179033000126	39.0	35.0	41.0	31.0	41.0
44	36.84542344584327	39.0	35.0	41.0	31.0	41.0
45	36.69199098665774	39.0	35.0	41.0	31.0	41.0
46	36.58996699290365	39.0	35.0	40.0	31.0	41.0
47	36.44153439430061	39.0	35.0	40.0	31.0	41.0
48	36.3751158908082	38.0	35.0	40.0	30.0	41.0
49	36.201097564376035	38.0	35.0	40.0	30.0	41.0
50	36.13800591130258	38.0	35.0	40.0	30.0	41.0
51	34.955196439276705	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	12.0
12	10.0
13	13.0
14	26.0
15	54.0
16	95.0
17	163.0
18	276.0
19	514.0
20	917.0
21	1537.0
22	2219.0
23	2839.0
24	3697.0
25	4677.0
26	5907.0
27	7542.0
28	9627.0
29	12608.0
30	16528.0
31	21974.0
32	28871.0
33	40990.0
34	67206.0
35	82737.0
36	96562.0
37	144817.0
38	244107.0
39	351003.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.95468582263304	32.26888061678308	20.549357285262175	25.227076275321707
2	23.519298869323965	30.013192382227054	20.022969035370224	26.444539713078758
3	21.700597404045897	29.837177771271627	20.942863217477377	27.519361607205095
4	21.675937931322935	30.13988804773656	19.201538472262886	28.982635548677628
5	19.034150319963196	33.26928841301044	17.565212541999525	30.131348725026836
6	20.85572727145984	36.34814992959415	19.7625196927238	23.033603106222202
7	86.95121781198154	4.9689273216501455	4.410037363893652	3.6698175024746607
8	88.40542961506824	3.950308112705118	3.47088613771662	4.1733761345100175
9	81.2505228156761	7.735232199868948	6.471151030992512	4.543093953462434
10	36.0632153861168	35.14541246671407	13.139055028092628	15.652317119076498
11	26.58962106319796	28.35647664059559	26.11664714821476	18.937255147991692
12	28.30628633568949	22.965898476166576	29.537604388863333	19.190210799280607
13	26.46318680552651	23.825233175791542	27.54924923668911	22.162330781992836
14	21.323908709412077	26.939559022404396	28.11763701256152	23.61889525562201
15	20.995841872656044	28.918329220516682	28.800347149608935	21.285481757218342
16	23.331608041602188	26.91350537454515	28.198324898573755	21.556561685278904
17	22.446481101956028	26.583870090760804	27.703915540870245	23.265733266412926
18	23.274969676690784	27.365828070322195	26.473294575264543	22.885907677722475
19	24.043857264349548	29.810427035844246	25.768190500090622	20.37752519971559
20	25.158064606075815	28.19571082019323	25.37947704490638	21.26674752882457
21	26.355138232464764	26.64390675756689	25.901247089659403	21.099707920308948
22	23.741408395722672	26.62804801539169	26.079440099265273	23.551103489620367
23	24.147026224434313	27.342649908681526	25.778036861990607	22.732287004893557
24	22.406485702733978	27.53766015586878	27.159838693936734	22.89601544746051
25	22.925990212890543	27.86407140965048	25.311336735120666	23.89860164233831
26	23.908970819914398	28.456247298785676	24.560573424233553	23.074208457066376
27	22.141853834678713	27.582186624283743	26.267305198879082	24.00865434215846
28	22.345316268629663	27.98205348055823	27.558311375074933	22.114318875737172
29	23.120477644401692	29.327171079230972	24.584884353172445	22.967466923194895
30	24.209938377458975	26.98565393784767	26.15725249905893	22.647155185634418
31	23.915157472081646	27.32835961353465	24.875482733140938	23.88100018124277
32	25.26027507075439	27.521191462071464	25.502948680413233	21.715584786760914
33	24.821632718502098	27.431354301727385	25.40265520654705	22.34435777322347
34	22.783174397367798	27.203929482621607	27.346745298144352	22.666150821866243
35	23.479390606047932	26.875949781811592	27.1780501066544	22.46660950548608
36	24.7550608557447	28.99631589220238	25.00531529270707	21.243307959345852
37	24.367480167858687	29.31200942462392	24.266663878316393	22.053846529201
38	24.319032581872936	26.823406806363014	26.572803825616575	22.284756786147476
39	24.060935909768986	26.719802166548163	25.595312783191826	23.62394914049103
40	24.306920685376497	27.0235580743653	27.006130885161795	21.663390355096407
41	21.757584312741365	26.713354106542862	28.794596177171776	22.734465403543993
42	22.618400323448633	27.91574302563888	25.925732290490334	23.54012436042216
43	21.91930862854992	27.331322235699247	26.4919416677123	24.257427468038532
44	22.77419939492799	26.09921995901125	26.410905237915987	24.715675408144772
45	23.30599007347303	26.335009828934712	26.836390062319627	23.52261003527263
46	22.232649490428987	26.91428959805931	27.567373513460762	23.285687398050943
47	22.567600066920406	27.152693546363295	26.96021024161055	23.31949614510575
48	22.615437701284037	26.187750080165074	28.299925411630213	22.896886806920687
49	21.780588202489998	27.513436362875904	27.237215414000303	23.46876002063379
50	21.494433755768398	25.904296847770013	27.800462169057678	24.800807227403908
51	22.382436181633135	25.314647901069332	28.103869533090748	24.199046384206785
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1187.0
1	1008.0
2	829.0
3	608.5
4	388.0
5	411.0
6	434.0
7	435.5
8	437.0
9	513.5
10	590.0
11	683.0
12	776.0
13	895.5
14	1015.0
15	1075.5
16	1136.0
17	1447.0
18	1758.0
19	2306.0
20	2854.0
21	3142.0
22	3430.0
23	4634.0
24	5838.0
25	6397.5
26	8749.0
27	10541.0
28	14348.0
29	18155.0
30	20611.0
31	23067.0
32	25813.5
33	28560.0
34	30968.5
35	33377.0
36	38812.5
37	44248.0
38	46717.5
39	49187.0
40	52109.5
41	55032.0
42	57309.0
43	59586.0
44	66274.5
45	72963.0
46	79406.5
47	85850.0
48	87799.5
49	89749.0
50	89934.5
51	90120.0
52	84169.5
53	78219.0
54	72682.5
55	67146.0
56	63080.0
57	59014.0
58	58672.0
59	58330.0
60	56212.5
61	54095.0
62	49589.0
63	45083.0
64	39141.5
65	33200.0
66	27777.5
67	22355.0
68	18178.5
69	14002.0
70	12193.0
71	10384.0
72	8829.0
73	7274.0
74	5905.5
75	3695.5
76	2854.0
77	2192.5
78	1531.0
79	1143.5
80	756.0
81	599.0
82	442.0
83	328.0
84	214.0
85	145.5
86	77.0
87	51.5
88	26.0
89	18.5
90	11.0
91	10.0
92	9.0
93	8.0
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1147632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.97690576986889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43758362947807	16.578846669316434
2	9.145511076483915	4.019800702903584
3	3.047286908815712	2.009098117463939
4	1.5627967345538378	1.3738174629099398
5	0.9331174753406012	1.0253517413889175
6	0.6456073888816292	0.8513071649869599
7	0.5062185529154634	0.7787582205467769
8	0.40305766021428757	0.7086368174682586
9	0.35618654773140995	0.7045090376389295
>10	5.649790113450166	31.08486986345565
>50	2.0121422147860315	30.882049663987726
>100	0.29430378889470754	8.465022392986937
>500	0.0035988235055059337	0.5009975776261708
>1k	0.002799084948726837	1.016934567319777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	2308	0.20110976340847936	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCC	2109	0.18376971015098917	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGT	2008	0.17496897960321775	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCT	1902	0.16573256932535865	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7427189203507744E-4	0.0	0.0	0.2322172961367407	0.0
2	1.7427189203507744E-4	0.0	0.0	0.3415729083887518	0.0
3	1.7427189203507744E-4	0.0	0.0	0.503994311765444	0.0
4	1.7427189203507744E-4	0.0	0.0	1.2495294658915053	0.0
5	1.7427189203507744E-4	0.0	0.0	1.3681214884213755	0.0
6	1.7427189203507744E-4	0.0	0.0	1.9977658343441103	0.0
7	1.7427189203507744E-4	0.0	0.0	2.428827359292874	0.0
8	1.7427189203507744E-4	0.0	0.0	2.817453678531097	0.0
9	1.7427189203507744E-4	0.0	0.0	3.464612349603357	0.0
10	1.7427189203507744E-4	0.0	0.0	4.29859048893722	0.0
11	2.614078380526162E-4	0.0	0.0	5.144331980983451	0.0
12	2.614078380526162E-4	0.0	0.0	5.505074797496062	0.0
13	2.614078380526162E-4	0.0	0.0	5.690674362513419	0.0
14	2.614078380526162E-4	0.0	0.0	5.8885600959192494	0.0
15	2.614078380526162E-4	0.0	0.0	6.042964992262328	0.0
16	2.614078380526162E-4	0.0	0.0	6.354475999275029	0.0
17	2.614078380526162E-4	0.0	0.0	6.706766629023938	0.0
18	2.614078380526162E-4	0.0	0.0	7.245963862980467	0.0
19	2.614078380526162E-4	0.0	0.0	7.44803212179514	0.0
20	2.614078380526162E-4	0.0	0.0	7.723207439318527	0.0
21	2.614078380526162E-4	0.0	0.0	8.007096351443668	0.0
22	2.614078380526162E-4	0.0	0.0	8.32392265116344	0.0
23	2.614078380526162E-4	0.0	0.0	8.631425404659334	0.0
24	2.614078380526162E-4	0.0	0.0	8.8712235280996	0.0
25	2.614078380526162E-4	0.0	0.0	9.080175526649658	0.0
26	2.614078380526162E-4	0.0	0.0	9.26237678977233	0.0
27	2.614078380526162E-4	0.0	0.0	9.499038019155966	0.0
28	2.614078380526162E-4	0.0	0.0	9.71417666987327	0.0
29	2.614078380526162E-4	0.0	0.0	9.933584981945431	0.0
30	2.614078380526162E-4	0.0	0.0	10.241610551117432	0.0
31	2.614078380526162E-4	0.0	0.0	10.47670333347275	0.0
32	2.614078380526162E-4	0.0	0.0	10.708310677987368	0.0
33	2.614078380526162E-4	0.0	0.0	10.93233719519846	0.0
34	2.614078380526162E-4	0.0	0.0	11.148957156998062	0.0
35	2.614078380526162E-4	0.0	0.0	11.39084654314275	0.0
36	2.614078380526162E-4	0.0	0.0	11.613304613325527	0.0
37	2.614078380526162E-4	0.0	0.0	11.838463897834846	0.0
38	2.614078380526162E-4	0.0	0.0	12.07129114559371	0.0
39	2.614078380526162E-4	0.0	0.0	12.33322179932243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	25	3.890801E-5	45.000004	1
CCGATAG	25	3.890801E-5	45.000004	1
ACGACCG	25	3.890801E-5	45.000004	1
TATCTCG	55	1.8189894E-12	45.0	1
CGTAGCA	20	7.0332823E-4	45.0	12
ACGTCTA	45	3.8562575E-10	45.0	43
GCCGTAT	20	7.0332823E-4	45.0	42
CGTATAG	30	2.1654996E-6	44.999996	1
TCGTCGA	30	2.1654996E-6	44.999996	40
TTACGCG	80	0.0	42.1875	1
TAATGCG	70	0.0	41.785717	1
GGCTACG	70	0.0	41.785717	1
ATATCCG	120	0.0	41.249996	1
TAAACCG	110	0.0	40.909092	1
ATCAGCG	160	0.0	40.78125	1
ATTAGCG	50	1.0822987E-9	40.500004	1
CTCGTAA	50	1.0822987E-9	40.500004	33
TATAGCG	50	1.0822987E-9	40.500004	1
TATACGG	95	0.0	40.263157	2
ATACGGG	620	0.0	39.919353	3
>>END_MODULE