SRR3553192_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478975
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	3112	0.6497207578683648	RNA PCR Primer, Index 21 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1066	0.22255858865285247	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	948	0.1979226473197975	RNA PCR Primer, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	840	0.17537449762513702	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGT	827	0.17266036849522418	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	691	0.14426640221305914	TruSeq Adapter, Index 21 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	639	0.13340988569340778	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	593	0.12380604415679315	RNA PCR Primer, Index 21 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	35	1.2099372E-7	45.000004	6
AGCGTGA	20	7.0291606E-4	45.000004	28
CCGATTG	20	7.0291606E-4	45.000004	1
ACCGCGT	20	7.0291606E-4	45.000004	41
CGTTGTC	20	7.0291606E-4	45.000004	18
CATTGAT	20	7.0291606E-4	45.000004	32
GCACGGT	35	1.2099372E-7	45.000004	3
TAGCCTA	20	7.0291606E-4	45.000004	30
ATGTACG	30	2.1628402E-6	45.000004	1
ATTCCGG	30	2.1628402E-6	45.000004	2
AAACGCC	30	2.1628402E-6	45.000004	27
TCGTGGC	20	7.0291606E-4	45.000004	22
AATTGCG	35	1.2099372E-7	45.000004	1
GAAACGC	30	2.1628402E-6	45.000004	26
GTTCCAC	30	2.1628402E-6	45.000004	9
ACGCTAG	20	7.0291606E-4	45.000004	1
GCGATAG	30	2.1628402E-6	45.000004	1
ACCACGC	20	7.0291606E-4	45.000004	33
AAGGGCG	40	6.8012014E-9	45.000004	5
GCATGTA	20	7.0291606E-4	45.000004	40