##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478975
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76934913095673	31.0	31.0	34.0	30.0	34.0
2	31.972221932251163	33.0	31.0	34.0	30.0	34.0
3	32.14616211702072	34.0	31.0	34.0	30.0	34.0
4	35.81137846442925	37.0	35.0	37.0	35.0	37.0
5	35.57346208048437	37.0	35.0	37.0	33.0	37.0
6	34.91350696800459	37.0	35.0	37.0	33.0	37.0
7	35.33272300224438	37.0	35.0	37.0	33.0	37.0
8	35.72385406336448	37.0	35.0	37.0	35.0	37.0
9	37.42612244897959	39.0	37.0	39.0	35.0	39.0
10	36.963309149746856	39.0	37.0	39.0	33.0	39.0
11	36.961304869774	39.0	37.0	39.0	33.0	39.0
12	36.853280442611826	39.0	37.0	39.0	33.0	39.0
13	36.828955582232894	39.0	37.0	39.0	33.0	39.0
14	37.9867592254293	40.0	37.0	41.0	33.0	41.0
15	38.00904431337752	40.0	37.0	41.0	33.0	41.0
16	37.90937940393549	40.0	37.0	41.0	33.0	41.0
17	37.919020825721596	40.0	37.0	41.0	33.0	41.0
18	37.89746855263844	40.0	37.0	41.0	33.0	41.0
19	37.91825878177358	40.0	37.0	41.0	33.0	41.0
20	37.821374810793884	40.0	37.0	41.0	33.0	41.0
21	37.806217443499136	40.0	37.0	41.0	33.0	41.0
22	37.79184091027715	40.0	37.0	41.0	33.0	41.0
23	37.67188266610992	39.0	36.0	41.0	33.0	41.0
24	37.532775197035335	39.0	36.0	41.0	32.0	41.0
25	37.58104285192338	39.0	36.0	41.0	32.0	41.0
26	37.52068062007412	39.0	36.0	41.0	32.0	41.0
27	37.437173130121614	39.0	36.0	41.0	32.0	41.0
28	37.413963150477585	39.0	36.0	41.0	32.0	41.0
29	37.20669554778433	39.0	36.0	41.0	32.0	41.0
30	37.22566731040242	39.0	36.0	41.0	32.0	41.0
31	37.20507959705621	39.0	36.0	41.0	32.0	41.0
32	37.17261652487082	39.0	36.0	41.0	32.0	41.0
33	36.91278041651443	39.0	35.0	41.0	31.0	41.0
34	36.952047601649355	39.0	35.0	41.0	31.0	41.0
35	36.88509421159768	39.0	35.0	41.0	31.0	41.0
36	36.85629312594603	39.0	35.0	41.0	31.0	41.0
37	36.82752544496059	39.0	35.0	41.0	31.0	41.0
38	36.64782086747743	39.0	35.0	40.0	30.0	41.0
39	36.56297510308471	39.0	35.0	40.0	30.0	41.0
40	36.481202567983715	39.0	35.0	40.0	30.0	41.0
41	36.49434730413905	39.0	35.0	40.0	30.0	41.0
42	36.458472780416514	39.0	35.0	40.0	30.0	41.0
43	36.385068114202205	39.0	35.0	40.0	30.0	41.0
44	36.30353149955634	38.0	35.0	40.0	30.0	41.0
45	36.07438801607599	38.0	35.0	40.0	30.0	41.0
46	35.90404718409103	38.0	35.0	40.0	30.0	41.0
47	35.787285348922175	38.0	35.0	40.0	29.0	41.0
48	35.74417036379769	38.0	35.0	40.0	29.0	41.0
49	35.656758703481394	38.0	34.0	40.0	29.0	41.0
50	35.55378673208414	38.0	34.0	40.0	28.0	41.0
51	34.29829740591889	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	4.0
12	3.0
13	16.0
14	22.0
15	36.0
16	68.0
17	115.0
18	190.0
19	325.0
20	549.0
21	823.0
22	1269.0
23	1652.0
24	2139.0
25	2570.0
26	3109.0
27	3929.0
28	4962.0
29	6327.0
30	8428.0
31	11338.0
32	14619.0
33	19961.0
34	30721.0
35	37824.0
36	45977.0
37	66470.0
38	100304.0
39	115203.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.986638133514276	31.476590636254503	21.103189101727647	27.433582128503573
2	23.821076256589592	31.182003236077037	20.16201263113941	24.834907876193956
3	21.373558118899734	30.433947492040293	20.930319954068587	27.262174434991387
4	20.43175531081998	31.158411190563186	19.62482384258051	28.785009656036326
5	19.054647946134974	33.48421107573464	18.86674669867947	28.594394279450913
6	20.980009395062375	36.440106477373554	19.407484733023644	23.172399394540424
7	83.20684795657394	5.728900255754476	6.191972441150373	4.872279346521218
8	84.61882144162013	4.758077143901039	5.0464011691633175	5.576700245315518
9	78.05960645127617	7.629625763348819	9.14014301372723	5.170624771647789
10	35.28827183047132	32.63969935800407	14.431233362910382	17.640795448614227
11	26.87551542356073	29.313012161386293	25.17897593820137	18.63249647685161
12	26.507855316039457	25.14181324703795	28.76517563547158	19.585155801451016
13	26.10345007568245	25.03408319849679	26.56088522365468	22.301581502166083
14	21.310924369747898	26.9849157054126	29.526175687666374	22.177984237173128
15	20.592097708648677	28.471632131113317	28.192703168223808	22.7435669920142
16	22.043321676496685	28.092489169580876	26.925413643718354	22.93877551020408
17	21.517824521112793	27.99519807923169	27.435043582650454	23.051933817005064
18	23.659272404614022	26.981784017955007	25.829531812725094	23.52941176470588
19	22.55650086121405	30.78615794143744	25.512187483689132	21.145153713659376
20	24.960384153661465	27.363014771125844	26.191972441150373	21.484628634062318
21	23.93882770499504	28.84743462602432	25.393600918628323	21.820136750352315
22	22.728534892217755	26.290307427318755	26.145623466778016	24.835534213685474
23	22.683438592828438	28.118586565060806	25.157471684325905	24.040503157784855
24	21.062894723106634	27.520434260660785	26.836473719922754	24.580197296309827
25	21.87462811211441	28.36640743253823	24.437183569079806	25.321780886267554
26	24.26974267968057	27.754893261652487	25.352262644188112	22.623101414478835
27	20.819249438906	26.024322772587293	26.789289628895034	26.36713815961167
28	21.329296936165772	27.36510256276424	26.40513596743045	24.900464533639543
29	23.328566209092333	27.44276841171251	25.865233049741637	23.36343232945352
30	21.490265671485986	27.456965394853594	26.585312385823894	24.467456547836523
31	23.55634427684117	27.54465264366616	24.03110809541208	24.86789498408059
32	25.06832298136646	25.446213267915862	25.436818205543087	24.04864554517459
33	21.605302990761523	25.591523565948123	25.98277571898325	26.820397724307117
34	20.343232945352057	25.394853593611355	28.57142857142857	25.69048488960802
35	21.33681298606399	25.52325277937262	27.8110548567253	25.328879377838092
36	21.60655566574456	27.97870452528838	27.586408476434055	22.828331332533015
37	22.003027297875672	27.463437548932617	25.317396523826925	25.21613862936479
38	23.024583746542095	26.487394957983195	26.869460827809384	23.61856046766533
39	23.180959340257843	25.588600657654364	26.205960645127618	25.024479356960178
40	23.63609791742784	26.38237903857195	26.244584790437912	23.736938253562297
41	20.304191241714076	24.604833237642882	28.52904640116916	26.561929119473877
42	22.89117386084869	24.714859856986273	26.934599926927294	25.459366355237744
43	21.308210240617985	23.8407014979905	28.714859856986273	26.136228404405244
44	24.26807244636985	22.45023226681977	26.28362649407589	26.998068792734486
45	24.550133096716948	23.08011900412339	27.06592202098231	25.30382587817736
46	20.73907823999165	25.361031369069366	29.10193642674461	24.797953964194374
47	21.55665744558693	25.824729891956782	27.0680098126207	25.550602849835585
48	21.29630982827914	23.740696278511404	30.54251265723681	24.420481235972648
49	22.236859961375856	23.911060076204393	27.879951980792317	25.972127981627434
50	21.104650555874525	24.29876298345425	27.51500600240096	27.081580458270267
51	20.854115559267186	23.24296675191816	29.93663552377473	25.966282165039928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1267.0
1	1068.5
2	870.0
3	576.0
4	282.0
5	280.5
6	279.0
7	307.0
8	335.0
9	363.5
10	392.0
11	477.0
12	562.0
13	608.0
14	654.0
15	761.5
16	869.0
17	821.5
18	774.0
19	896.5
20	1019.0
21	1111.0
22	1203.0
23	1647.5
24	2092.0
25	2236.5
26	3219.5
27	4058.0
28	4706.0
29	5354.0
30	6064.5
31	6775.0
32	9005.0
33	11235.0
34	11941.5
35	12648.0
36	13363.0
37	14078.0
38	15580.5
39	17083.0
40	17976.5
41	18870.0
42	21247.5
43	23625.0
44	27224.0
45	30823.0
46	34624.5
47	38426.0
48	43046.5
49	47667.0
50	45803.0
51	43939.0
52	41086.0
53	38233.0
54	33401.5
55	28570.0
56	26871.5
57	25173.0
58	23941.0
59	22709.0
60	21994.0
61	21279.0
62	18473.5
63	15668.0
64	13519.5
65	11371.0
66	10059.5
67	8748.0
68	7981.5
69	7215.0
70	5749.5
71	4284.0
72	3653.0
73	3022.0
74	2599.0
75	1766.0
76	1356.0
77	1079.0
78	802.0
79	617.5
80	433.0
81	328.0
82	223.0
83	148.0
84	73.0
85	58.0
86	43.0
87	31.0
88	19.0
89	15.0
90	11.0
91	7.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.3581592939811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00560067160465	21.553789310800816
2	10.02342190635569	5.6849159018242865
3	3.5624034257206416	3.0306961144802975
4	1.6498012469309677	1.871413062555081
5	0.9538263583361163	1.3524379904246742
6	0.6673667189430286	1.1355175033972753
7	0.47877067446187116	0.9503938536173557
8	0.3820307618950001	0.8666951360815504
9	0.30991097758295133	0.7909654382325665
>10	4.563773878722754	32.246291979200606
>50	1.303509421371217	24.607942696811495
>100	0.09363864714525277	4.072064991973551
>500	0.004458983197392989	0.9563725770404707
>1k	0.0014863277324643296	0.8805034435599576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	3112	0.6497207578683648	RNA PCR Primer, Index 21 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1066	0.22255858865285247	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	948	0.1979226473197975	RNA PCR Primer, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	840	0.17537449762513702	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGT	827	0.17266036849522418	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	691	0.14426640221305914	TruSeq Adapter, Index 21 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	639	0.13340988569340778	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	593	0.12380604415679315	RNA PCR Primer, Index 21 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7232110235398507	0.0
2	0.0	0.0	0.0	1.0194686570280287	0.0
3	0.0	0.0	0.0	1.3785688188318805	0.0
4	0.0	0.0	0.0	2.988256172034031	0.0
5	0.0	0.0	0.0	3.2114410981784016	0.0
6	0.0	0.0	0.0	4.409833498616838	0.0
7	0.0	0.0	0.0	5.325956469544339	0.0
8	0.0	0.0	0.0	6.11368025471058	0.0
9	0.0	0.0	0.0	7.211649877342241	0.0
10	0.0	0.0	0.0	8.811733389007777	0.0
11	0.0	0.0	0.0	10.241661882144163	0.0
12	0.0	0.0	0.0	10.935017485254972	0.0
13	0.0	0.0	0.0	11.231483897906989	0.0
14	0.0	0.0	0.0	11.576595855733597	0.0
15	0.0	0.0	0.0	11.880578318283835	0.0
16	0.0	0.0	0.0	12.459731718774467	0.0
17	0.0	0.0	0.0	13.129286497207579	0.0
18	0.0	0.0	0.0	14.028707135027924	0.0
19	0.0	0.0	0.0	14.40555352575813	0.0
20	0.0	0.0	0.0	14.84503366564017	0.0
21	0.0	0.0	0.0	15.354663604572263	0.0
22	0.0	0.0	0.0	15.870139360091862	0.0
23	0.0	0.0	0.0	16.358891382640014	0.0
24	0.0	0.0	0.0	16.760373714703274	0.0
25	0.0	0.0	0.0	17.08377263949058	0.0
26	0.0	0.0	0.0	17.383788297927868	0.0
27	0.0	0.0	0.0	17.721801764183933	0.0
28	0.0	0.0	0.0	18.058562555456966	0.0
29	0.0	0.0	0.0	18.398663813351426	0.0
30	0.0	0.0	0.0	18.873010073594656	0.0
31	0.0	0.0	0.0	19.194738765071246	0.0
32	0.0	0.0	0.0	19.53776293125946	0.0
33	0.0	0.0	0.0	19.89853332637403	0.0
34	0.0	0.0	0.0	20.23487655931938	0.0
35	0.0	0.0	0.0	20.59042747533796	0.0
36	0.0	0.0	0.0	20.900046975311863	0.0
37	0.0	0.0	0.0	21.199436296257634	0.0
38	0.0	0.0	0.0	21.521582546061904	0.0
39	0.0	0.0	0.0	21.859178453990292	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	35	1.2099372E-7	45.000004	6
AGCGTGA	20	7.0291606E-4	45.000004	28
CCGATTG	20	7.0291606E-4	45.000004	1
ACCGCGT	20	7.0291606E-4	45.000004	41
CGTTGTC	20	7.0291606E-4	45.000004	18
CATTGAT	20	7.0291606E-4	45.000004	32
GCACGGT	35	1.2099372E-7	45.000004	3
TAGCCTA	20	7.0291606E-4	45.000004	30
ATGTACG	30	2.1628402E-6	45.000004	1
ATTCCGG	30	2.1628402E-6	45.000004	2
AAACGCC	30	2.1628402E-6	45.000004	27
TCGTGGC	20	7.0291606E-4	45.000004	22
AATTGCG	35	1.2099372E-7	45.000004	1
GAAACGC	30	2.1628402E-6	45.000004	26
GTTCCAC	30	2.1628402E-6	45.000004	9
ACGCTAG	20	7.0291606E-4	45.000004	1
GCGATAG	30	2.1628402E-6	45.000004	1
ACCACGC	20	7.0291606E-4	45.000004	33
AAGGGCG	40	6.8012014E-9	45.000004	5
GCATGTA	20	7.0291606E-4	45.000004	40
>>END_MODULE