##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801216
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.950417864845434	33.0	31.0	34.0	30.0	34.0
2	32.1608517553319	34.0	31.0	34.0	30.0	34.0
3	32.31333248462337	34.0	31.0	34.0	30.0	34.0
4	35.94152762800543	37.0	35.0	37.0	35.0	37.0
5	35.73069309649333	37.0	35.0	37.0	35.0	37.0
6	35.03960480070293	37.0	35.0	37.0	33.0	37.0
7	35.46442657161115	37.0	35.0	37.0	35.0	37.0
8	35.87925727893602	37.0	35.0	37.0	35.0	37.0
9	37.55438982746226	39.0	37.0	39.0	35.0	39.0
10	37.11476555635434	39.0	37.0	39.0	33.0	39.0
11	37.08731478153207	39.0	37.0	39.0	33.0	39.0
12	36.92677879622973	39.0	37.0	39.0	33.0	39.0
13	36.92294087986261	39.0	37.0	39.0	33.0	39.0
14	38.14238232886013	40.0	37.0	41.0	33.0	41.0
15	38.15923546209761	40.0	37.0	41.0	33.0	41.0
16	38.059596912692705	40.0	37.0	41.0	33.0	41.0
17	38.05432118180366	40.0	37.0	41.0	33.0	41.0
18	38.05075784807093	40.0	37.0	41.0	33.0	41.0
19	38.091850387411135	40.0	37.0	41.0	33.0	41.0
20	38.00464793513859	40.0	37.0	41.0	33.0	41.0
21	38.0000686456586	40.0	37.0	41.0	33.0	41.0
22	37.98334781132678	40.0	37.0	41.0	33.0	41.0
23	37.88543538820992	40.0	37.0	41.0	33.0	41.0
24	37.750245876268075	40.0	36.0	41.0	33.0	41.0
25	37.77761926871156	40.0	36.0	41.0	33.0	41.0
26	37.72878849149293	40.0	36.0	41.0	33.0	41.0
27	37.65178428788242	40.0	36.0	41.0	33.0	41.0
28	37.571729720824344	40.0	36.0	41.0	33.0	41.0
29	37.38399258127646	39.0	36.0	41.0	32.0	41.0
30	37.45710769630162	39.0	36.0	41.0	32.0	41.0
31	37.40465866882339	39.0	36.0	41.0	32.0	41.0
32	37.37025845714514	39.0	36.0	41.0	32.0	41.0
33	37.14737973280614	39.0	35.0	41.0	32.0	41.0
34	37.1092302200655	39.0	35.0	41.0	31.0	41.0
35	37.06330253015417	39.0	35.0	41.0	31.0	41.0
36	37.00909742191868	39.0	35.0	41.0	31.0	41.0
37	36.985103892084034	39.0	35.0	41.0	31.0	41.0
38	36.8091401070373	39.0	35.0	41.0	31.0	41.0
39	36.809420930186114	39.0	35.0	41.0	31.0	41.0
40	36.75126557632399	39.0	35.0	41.0	31.0	41.0
41	36.728208123652045	39.0	35.0	41.0	31.0	41.0
42	36.65484213994728	39.0	35.0	41.0	31.0	41.0
43	36.58456021846793	39.0	35.0	40.0	31.0	41.0
44	36.48807312884416	39.0	35.0	40.0	31.0	41.0
45	36.261534966850384	38.0	35.0	40.0	30.0	41.0
46	36.12073148813803	38.0	35.0	40.0	30.0	41.0
47	36.00424230170142	38.0	35.0	40.0	30.0	41.0
48	35.93437100607077	38.0	35.0	40.0	30.0	41.0
49	35.83956386292835	38.0	35.0	40.0	29.0	41.0
50	35.74381689831456	38.0	34.0	40.0	29.0	41.0
51	34.59956366323189	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	6.0
11	6.0
12	9.0
13	11.0
14	22.0
15	39.0
16	79.0
17	104.0
18	209.0
19	430.0
20	770.0
21	1217.0
22	1762.0
23	2243.0
24	2855.0
25	3617.0
26	4676.0
27	5824.0
28	7433.0
29	9697.0
30	12777.0
31	17149.0
32	22828.0
33	31985.0
34	51738.0
35	63579.0
36	74011.0
37	108152.0
38	166968.0
39	210946.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.948533229491172	31.46305116223341	20.585210480070295	27.003205128205128
2	25.334241952232606	29.160925393402024	19.725766834411694	25.779065819953672
3	21.774153287003752	29.930006390286763	21.191414050643022	27.104426272066455
4	20.906721782890006	30.9590672178289	19.39314741592779	28.741063583353306
5	18.939337207444684	32.87802540138989	17.329409297867244	30.853228093298185
6	20.40198897675533	36.762371195782414	19.796908698777855	23.0387311286844
7	87.14067117980669	4.770498841760524	4.517009146097931	3.571820832334851
8	88.82086728173176	3.673041976196182	3.583178568575765	3.9229121734962855
9	82.17858854541097	6.622683521048008	6.809524522725457	4.38920341081556
10	38.49224179247544	31.255616462976278	13.560637830497644	16.691503914050642
11	29.569679087786565	29.464214394120937	22.235826543653648	18.730279974438854
12	27.64522925153766	24.955193106478156	27.96461877945523	19.434958862528955
13	26.96351545650611	25.449816279255533	25.362573887690708	22.224094376547647
14	22.356892523364486	27.076094336608353	27.837312285326306	22.729700854700855
15	22.470844316638708	28.46685537982267	26.953655443725538	22.108644859813083
16	24.91350647016535	27.40871375509226	25.576124291077562	22.10165548366483
17	22.94050543174375	28.251557632398754	25.993739515935776	22.81419741992172
18	26.049155283968368	25.7281432223021	25.440829938493494	22.78187155523604
19	24.58637870436936	29.034492571291636	24.49951074366962	21.879617980669384
20	27.110292355619457	27.680800183720745	23.80681364326224	21.402093817397557
21	25.90737079638949	28.13685697739436	24.991512900391406	20.964259325824745
22	24.462067657161114	26.614046649093375	25.353213116063582	23.570672577681922
23	24.027353422797347	27.35192507388769	24.79468707564502	23.826034427669942
24	22.58017812924355	27.156347352024923	26.26882139148494	23.994653127246586
25	23.91876847192268	28.20987099608595	23.984543493889287	23.886817038102084
26	25.277702891604758	27.923306574007505	24.196221742950716	22.60276879143702
27	22.86224938094097	26.568990134994806	25.89226875149772	24.6764917325665
28	22.090048126847194	29.41092040099049	26.097456865564343	22.401574606597972
29	23.230564541896317	30.011881939452035	24.09175553159198	22.66579798705967
30	24.771597172298108	26.93743010623852	25.739750579119736	22.551222142343637
31	25.060657800143783	28.16406562025721	23.624840242830896	23.150436336768113
32	25.44657121175813	27.159467609233968	25.219790917804936	22.17417026120297
33	23.48854740793993	27.08071231727774	24.867576284048244	24.563163990734086
34	22.07382278936017	28.417056074766357	26.330976915089067	23.178144220784407
35	23.28261043214314	28.447135354261523	26.183326343957187	22.086927869638153
36	22.87697699496765	31.501991972202255	23.812305295950157	21.808725736879943
37	24.621450395398995	29.32867042096014	22.990179926511704	23.05969925712916
38	23.79345894240754	28.830552560108636	25.293179367361613	22.082809130122214
39	23.7150780813164	26.397625609074204	24.370581715791996	25.516714593817397
40	24.329019889767554	26.232751218148415	24.530089264318235	24.908139627765795
41	21.59966850387411	26.314252336448597	25.912987259365767	26.17309190031153
42	24.050193705567537	27.58594436456586	24.148669023084913	24.21519290678169
43	23.07030813163991	25.51197180285965	26.604436256889528	24.81328380861091
44	24.441723580158158	25.422857256969404	24.781082754213596	25.35433640865884
45	24.649657520568734	25.284068216311205	26.2155273983545	23.850746864765558
46	21.638858934419684	26.350946561226934	27.120401988976756	24.889792515376627
47	22.543858335330295	27.807732246984585	25.488257848070933	24.160151569614186
48	22.414055635434142	25.90312724658519	27.907206046808852	23.775611071171816
49	23.74153786244908	26.03754792715073	27.023549205208084	23.197365005192108
50	22.050358455148174	26.041417046089943	26.34969845834332	25.55852604041856
51	22.641584791117502	24.25488257848071	27.493335130601487	25.610197499800304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1060.0
1	899.0
2	738.0
3	528.5
4	319.0
5	317.0
6	315.0
7	316.5
8	318.0
9	375.0
10	432.0
11	517.5
12	603.0
13	662.0
14	721.0
15	750.5
16	780.0
17	796.0
18	812.0
19	1019.0
20	1226.0
21	1557.0
22	1888.0
23	2677.5
24	3467.0
25	3638.0
26	5266.5
27	6724.0
28	7960.0
29	9196.0
30	10867.5
31	12539.0
32	14694.0
33	16849.0
34	19207.0
35	21565.0
36	23950.5
37	26336.0
38	28242.0
39	30148.0
40	32012.0
41	33876.0
42	35724.5
43	37573.0
44	43299.5
45	49026.0
46	54155.0
47	59284.0
48	63593.5
49	67903.0
50	65825.5
51	63748.0
52	61562.0
53	59376.0
54	56654.5
55	53933.0
56	50896.0
57	47859.0
58	45095.0
59	42331.0
60	41121.0
61	39911.0
62	35056.0
63	30201.0
64	26807.0
65	23413.0
66	19656.0
67	15899.0
68	14373.0
69	12847.0
70	10400.5
71	7954.0
72	6864.5
73	5775.0
74	5048.5
75	3714.5
76	3107.0
77	2319.0
78	1531.0
79	1207.5
80	884.0
81	610.5
82	337.0
83	251.0
84	165.0
85	109.0
86	53.0
87	43.0
88	33.0
89	26.5
90	20.0
91	12.0
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.116991916098325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35218124459661	18.172679459363124
2	9.165867741467599	4.42106316450001
3	3.1298900410504613	2.2645059845487188
4	1.5078671602063265	1.4546088045298446
5	0.9198235273513554	1.1091688286684847
6	0.704225498604857	1.019028039417819
7	0.5496662540558708	0.9279407623932241
8	0.4356364441717325	0.8404992481957995
9	0.3716727295701113	0.8067265393028917
>10	6.013627184800202	35.779993523389535
>50	1.7173186844105408	27.4983259416739
>100	0.12693455012581142	4.470998737875976
>500	0.003173363753145286	0.4810035444633774
>1k	0.0021155758354301905	0.7534574216773191
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	2516	0.3140226855180126	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	1176	0.1467768991133477	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGT	1132	0.14128524642543333	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1083	0.13516954229571054	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	830	0.10359253934020289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3523394440450515	0.0
2	0.0	0.0	0.0	0.5097252176691429	0.0
3	0.0	0.0	0.0	0.7231508107676332	0.0
4	0.0	0.0	0.0	1.6215352663950795	0.0
5	0.0	0.0	0.0	1.7467199856218547	0.0
6	0.0	0.0	0.0	2.4759865005192108	0.0
7	0.0	0.0	0.0	2.954883576963016	0.0
8	0.0	0.0	0.0	3.388349708443166	0.0
9	0.0	0.0	0.0	4.0612019730010385	0.0
10	0.0	0.0	0.0	4.982551521687036	0.0
11	0.0	0.0	0.0	5.931484144100967	0.0
12	1.2481028836169023E-4	0.0	0.0	6.361455587506989	0.0
13	1.2481028836169023E-4	0.0	0.0	6.547797348030993	0.0
14	1.2481028836169023E-4	0.0	0.0	6.7605988896876745	0.0
15	1.2481028836169023E-4	0.0	0.0	6.931588984743191	0.0
16	1.2481028836169023E-4	0.0	0.0	7.295785406182603	0.0
17	1.2481028836169023E-4	0.0	0.0	7.725881659876987	0.0
18	1.2481028836169023E-4	0.0	0.0	8.353927030913011	0.0
19	1.2481028836169023E-4	0.0	0.0	8.613906861570413	0.0
20	1.2481028836169023E-4	0.0	0.0	8.963250858694783	0.0
21	1.2481028836169023E-4	0.0	0.0	9.289504952472242	0.0
22	1.2481028836169023E-4	0.0	0.0	9.659068216311207	0.0
23	1.2481028836169023E-4	0.0	0.0	10.011033229491174	0.0
24	1.2481028836169023E-4	0.0	0.0	10.290233844556274	0.0
25	1.2481028836169023E-4	0.0	0.0	10.546344556274462	0.0
26	1.2481028836169023E-4	0.0	0.0	10.765636232925953	0.0
27	1.2481028836169023E-4	0.0	0.0	11.043339124530714	0.0
28	1.2481028836169023E-4	0.0	0.0	11.293209321830817	0.0
29	1.2481028836169023E-4	0.0	0.0	11.543578760284367	0.0
30	1.2481028836169023E-4	0.0	0.0	11.933111670261203	0.0
31	1.2481028836169023E-4	0.0	0.0	12.183730729291478	0.0
32	1.2481028836169023E-4	0.0	0.0	12.454818675613069	0.0
33	1.2481028836169023E-4	0.0	0.0	12.723160795590703	0.0
34	1.2481028836169023E-4	0.0	0.0	12.984139108554997	0.0
35	1.2481028836169023E-4	0.0	0.0	13.263214913331735	0.0
36	1.2481028836169023E-4	0.0	0.0	13.505346872753416	0.0
37	1.2481028836169023E-4	0.0	0.0	13.755217070053519	0.0
38	1.2481028836169023E-4	0.0	0.0	14.032046289639748	0.0
39	1.2481028836169023E-4	0.0	0.0	14.329219586228932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACG	45	3.8380676E-10	45.000004	1
TTTAGCG	40	6.8102963E-9	45.0	1
CGAAACG	20	7.032005E-4	45.0	1
CGACACG	40	6.8102963E-9	45.0	1
TCTAGCG	35	1.2113196E-7	45.0	1
CCGTCCG	25	3.8897444E-5	45.0	1
AAACGCG	35	1.2113196E-7	45.0	1
ACGATAG	70	0.0	45.0	1
AATTGCG	25	3.8897444E-5	45.0	1
CGTAGGT	20	7.032005E-4	45.0	19
TCGTAAG	25	3.8897444E-5	45.0	1
ACTCCCG	25	3.8897444E-5	45.0	1
ATCGGCG	20	7.032005E-4	45.0	44
GCCGTTT	20	7.032005E-4	45.0	26
ATTACCG	60	0.0	44.999996	1
CGTAGCG	30	2.1646756E-6	44.999996	1
TACGACG	65	0.0	44.999996	1
TCATACG	30	2.1646756E-6	44.999996	1
TACTACG	65	0.0	41.53846	1
ACGGGTC	115	0.0	41.086956	5
>>END_MODULE