##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812617
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955982953839264	33.0	31.0	34.0	30.0	34.0
2	32.16106234548379	34.0	31.0	34.0	30.0	34.0
3	32.30245367743968	34.0	31.0	34.0	30.0	34.0
4	35.93183504652253	37.0	35.0	37.0	35.0	37.0
5	35.73974701489139	37.0	35.0	37.0	35.0	37.0
6	35.763508516312115	37.0	35.0	37.0	35.0	37.0
7	35.84484326564667	37.0	35.0	37.0	35.0	37.0
8	35.884040082843455	37.0	35.0	37.0	35.0	37.0
9	37.58422356411446	39.0	37.0	39.0	35.0	39.0
10	37.13817948676929	39.0	37.0	39.0	34.0	39.0
11	37.10173550393359	39.0	37.0	39.0	33.0	39.0
12	36.963749220112305	39.0	37.0	39.0	33.0	39.0
13	36.952083207710395	39.0	37.0	39.0	33.0	39.0
14	38.16364166636927	40.0	37.0	41.0	33.0	41.0
15	38.186139349779786	40.0	37.0	41.0	33.0	41.0
16	38.08233275946725	40.0	37.0	41.0	33.0	41.0
17	38.05104003485037	40.0	37.0	41.0	33.0	41.0
18	38.021337235130446	40.0	37.0	41.0	33.0	41.0
19	38.075454980636636	40.0	37.0	41.0	33.0	41.0
20	37.999040138220096	40.0	37.0	41.0	33.0	41.0
21	37.98540394798534	40.0	37.0	41.0	33.0	41.0
22	37.95944337861502	40.0	37.0	41.0	33.0	41.0
23	37.83829282429484	40.0	37.0	41.0	33.0	41.0
24	37.71498011978583	40.0	36.0	41.0	33.0	41.0
25	37.72961801192936	40.0	36.0	41.0	33.0	41.0
26	37.67530091051504	40.0	36.0	41.0	33.0	41.0
27	37.62380924839131	40.0	36.0	41.0	33.0	41.0
28	37.53986933573873	40.0	36.0	41.0	33.0	41.0
29	37.40114838847821	39.0	36.0	41.0	32.0	41.0
30	37.44776813677292	39.0	36.0	41.0	32.0	41.0
31	37.3839939356425	39.0	36.0	41.0	32.0	41.0
32	37.322812591909845	39.0	36.0	41.0	32.0	41.0
33	37.25400649998708	39.0	35.0	41.0	32.0	41.0
34	37.15864792392972	39.0	35.0	41.0	32.0	41.0
35	37.08975938234125	39.0	35.0	41.0	31.0	41.0
36	36.99806058696778	39.0	35.0	41.0	31.0	41.0
37	36.97841787705647	39.0	35.0	41.0	31.0	41.0
38	36.89061636662782	39.0	35.0	41.0	31.0	41.0
39	36.84542287449069	39.0	35.0	41.0	31.0	41.0
40	36.75587146220175	39.0	35.0	41.0	31.0	41.0
41	36.682198378818065	39.0	35.0	41.0	31.0	41.0
42	36.610849883770584	39.0	35.0	40.0	31.0	41.0
43	36.53347887135022	39.0	35.0	40.0	31.0	41.0
44	36.446725825327306	39.0	35.0	40.0	31.0	41.0
45	36.231909989576884	38.0	35.0	40.0	30.0	41.0
46	36.10031417014412	38.0	35.0	40.0	30.0	41.0
47	35.94813792967659	38.0	35.0	40.0	30.0	41.0
48	35.91082145709479	38.0	35.0	40.0	30.0	41.0
49	35.75091340693094	38.0	34.0	40.0	29.0	41.0
50	35.66839975043594	38.0	34.0	40.0	29.0	41.0
51	34.45645488588105	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	2.0
11	3.0
12	8.0
13	19.0
14	18.0
15	33.0
16	70.0
17	130.0
18	235.0
19	424.0
20	814.0
21	1160.0
22	1711.0
23	2243.0
24	2969.0
25	3574.0
26	4612.0
27	5884.0
28	7727.0
29	10061.0
30	13120.0
31	17368.0
32	23175.0
33	32180.0
34	52611.0
35	63568.0
36	73300.0
37	107819.0
38	171443.0
39	216280.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.974825778933987	31.639874627284442	20.424381965919984	26.960917627861587
2	25.256547672519776	29.377062010762756	19.72589793223622	25.64049238448125
3	21.878695621676634	29.91901473880069	21.07954916030553	27.122740479217143
4	20.920679729811216	30.915425101862255	19.384900881965304	28.778994286361225
5	18.95628567947754	32.85976050217999	17.324151476033606	30.85980234230886
6	20.780515298104767	35.405609284570716	20.27068102193284	23.54319439539168
7	87.02476074214543	4.759437717891701	4.623334239869459	3.592467300093402
8	88.46762989206476	3.7526903818157873	3.683531109981701	4.096148616137738
9	82.04664682131927	6.597819144812378	6.926141097220462	4.429392936647892
10	38.46461494160226	31.18763205790674	13.634467405924317	16.713285594566692
11	29.596968805722746	29.343466848466125	22.30829529778481	18.751269048026316
12	27.5118536776858	24.936962923492864	28.119643079088917	19.43154031973242
13	27.066625482853546	25.33272131889931	25.306632767958337	22.294020430288807
14	22.30324987048019	26.959687035836073	27.989569501991713	22.747493591692027
15	22.40329700215477	28.577423312581452	26.86936158116308	22.149918104100703
16	25.001938182440192	27.16470366728729	25.770442902375905	22.062915247896612
17	22.948326210256493	28.11201340853071	26.051387061801563	22.888273319411233
18	26.008070222503342	25.713589550796996	25.440521180334645	22.837819046365016
19	24.471060782631916	29.036680256504603	24.627222910670092	21.86503605019339
20	27.013340848148637	27.734467775101923	23.907326575742324	21.344864801007116
21	25.76576665267894	28.103153145946987	25.074050875135516	21.05702932623856
22	24.290163754880837	26.579434099951143	25.460702889553133	23.669699255614884
23	24.021771634115456	27.257121128403668	24.83506990378001	23.88603733370087
24	22.53583176330301	27.226602446170826	26.265879251849274	23.97168653867689
25	23.99174518869283	28.083955910348912	23.986207524578	23.938091376380264
26	25.205231985055693	27.887553423076305	24.208944681196677	22.698269910671325
27	22.806808127321972	26.5976468619288	25.947155917240227	24.648389093508996
28	22.12321425714697	29.33842142116151	26.126453175358133	22.41191114633339
29	23.190998957688556	30.005648417397126	24.071979788756572	22.73137283615775
30	24.771448296060754	27.061826173954028	25.691192775932574	22.475532754052647
31	25.037748410382747	28.146716103650306	23.564483637433135	23.25105184853381
32	25.396958222631326	27.235585767957105	25.125120444194497	22.24233556521707
33	23.42419614652413	27.01863239385836	24.89192325535892	24.665248204258585
34	21.915367264037055	28.393326745564025	26.332577339632323	23.358728650766597
35	23.360082301994666	28.409324441895752	26.019391669138102	22.211201586971477
36	22.751308426971132	31.42452102281887	24.018941272456768	21.805229277753234
37	24.70056619539094	29.336329414718126	22.870675853446336	23.092428536444597
38	23.625397942696253	28.86132089286835	25.39252809133946	22.120753073095937
39	23.652224848852534	26.265510074241615	24.371259769362442	25.711005307543406
40	24.277734775423106	26.251235206745616	24.500225813636682	24.970804204194597
41	21.561941234308414	26.146881002981726	26.030220878962663	26.2609568837472
42	23.98276186690655	27.53511186696808	24.163166657847423	24.318959608277947
43	23.13943715181937	25.31610832655482	26.653146562279645	24.89130795934616
44	24.505148181738754	25.255932376507012	24.78523092674655	25.453688515007684
45	24.54194288330173	25.215445898867483	26.301812538994383	23.9407986788364
46	21.69336846263369	26.174200145948213	27.201621428052825	24.930809963365274
47	22.49448387124562	27.728807051784543	25.451596508564307	24.325112568405537
48	22.368963484642826	25.743000700206863	28.064020319535526	23.824015495614788
49	23.774176518581324	25.819789642599154	27.059241930700438	23.346791908119076
50	22.005569659507493	25.822250826650194	26.284953428244794	25.88722608559752
51	22.56819633357412	24.182979189458255	27.534250452550218	25.71457402441741
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1028.0
1	846.0
2	664.0
3	480.0
4	296.0
5	297.0
6	298.0
7	329.5
8	361.0
9	402.0
10	443.0
11	508.0
12	573.0
13	632.5
14	692.0
15	713.0
16	734.0
17	775.5
18	817.0
19	1076.0
20	1335.0
21	1656.5
22	1978.0
23	2711.5
24	3445.0
25	3566.0
26	5206.0
27	6725.0
28	8027.0
29	9329.0
30	10927.5
31	12526.0
32	14660.5
33	16795.0
34	19124.5
35	21454.0
36	23934.5
37	26415.0
38	28501.0
39	30587.0
40	32300.0
41	34013.0
42	36086.0
43	38159.0
44	44004.5
45	49850.0
46	55239.5
47	60629.0
48	65363.5
49	70098.0
50	67702.5
51	65307.0
52	62885.0
53	60463.0
54	57599.5
55	54736.0
56	51595.5
57	48455.0
58	45705.0
59	42955.0
60	41572.0
61	40189.0
62	35247.0
63	30305.0
64	26834.5
65	23364.0
66	19729.5
67	16095.0
68	14547.5
69	13000.0
70	10650.5
71	8301.0
72	7048.0
73	5795.0
74	5059.0
75	3782.5
76	3242.0
77	2406.5
78	1571.0
79	1244.5
80	918.0
81	633.0
82	348.0
83	252.5
84	157.0
85	113.5
86	70.0
87	59.0
88	48.0
89	34.0
90	20.0
91	15.0
92	10.0
93	10.5
94	11.0
95	6.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	812617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.230180363647175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58083700383273	18.313373126382853
2	9.134248167960616	4.4264896119199895
3	3.1119419489928393	2.2620874411588865
4	1.5195064049817002	1.4727165702569482
5	0.9230555552741021	1.1182901294978995
6	0.6769939728407744	0.9842211640220413
7	0.5183680094764512	0.8792105255051428
8	0.42927896253951126	0.8321205350921358
9	0.3842902366352576	0.8380279571094851
>10	5.860940998027884	35.01481026286545
>50	1.7279356757302733	28.022784803451216
>100	0.12748325429829327	4.537643752503213
>500	0.0030718856457420066	0.4870356326843352
>1k	0.0020479237638280044	0.8111884875503994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	2743	0.3375513925994657	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	1318	0.1621920289632139	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1248	0.15357788478459103	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGT	1230	0.15136281913865943	No Hit
AGGCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTC	855	0.10521561818175106	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2305920255175563E-4	0.0	0.0	0.38615977760740916	0.0
2	1.2305920255175563E-4	0.0	0.0	0.551920523444624	0.0
3	1.2305920255175563E-4	0.0	0.0	0.7773649825194403	0.0
4	1.2305920255175563E-4	0.0	0.0	1.723198013332234	0.0
5	1.2305920255175563E-4	0.0	0.0	1.8674233987228916	0.0
6	1.2305920255175563E-4	0.0	0.0	2.6461420324704013	0.0
7	1.2305920255175563E-4	0.0	0.0	3.148100519679012	0.0
8	1.2305920255175563E-4	0.0	0.0	3.590498352852574	0.0
9	1.2305920255175563E-4	0.0	0.0	4.312732812628828	0.0
10	1.2305920255175563E-4	0.0	0.0	5.258811961846725	0.0
11	1.2305920255175563E-4	0.0	0.0	6.285864066343677	0.0
12	2.4611840510351126E-4	0.0	0.0	6.737737458113724	0.0
13	2.4611840510351126E-4	0.0	0.0	6.938323958273085	0.0
14	2.4611840510351126E-4	0.0	0.0	7.154662036359072	0.0
15	2.4611840510351126E-4	0.0	0.0	7.337281892945877	0.0
16	2.4611840510351126E-4	0.0	0.0	7.7276256834400465	0.0
17	2.4611840510351126E-4	0.0	0.0	8.18835933779382	0.0
18	2.4611840510351126E-4	0.0	0.0	8.865061892625924	0.0
19	2.4611840510351126E-4	0.0	0.0	9.124716810010128	0.0
20	2.4611840510351126E-4	0.0	0.0	9.482696030233186	0.0
21	2.4611840510351126E-4	0.0	0.0	9.827630974985755	0.0
22	2.4611840510351126E-4	0.0	0.0	10.212683219770199	0.0
23	2.4611840510351126E-4	0.0	0.0	10.570047143980497	0.0
24	2.4611840510351126E-4	0.0	0.0	10.845207520886223	0.0
25	2.4611840510351126E-4	0.0	0.0	11.09440240605353	0.0
26	2.4611840510351126E-4	0.0	0.0	11.317754858684964	0.0
27	3.6917760765526686E-4	0.0	0.0	11.60202161657952	0.0
28	3.6917760765526686E-4	0.0	0.0	11.847647784872825	0.0
29	3.6917760765526686E-4	0.0	0.0	12.126869115462759	0.0
30	3.6917760765526686E-4	0.0	0.0	12.514136425893133	0.0
31	3.6917760765526686E-4	0.0	0.0	12.771945455239061	0.0
32	3.6917760765526686E-4	0.0	0.0	13.03959922078913	0.0
33	3.6917760765526686E-4	0.0	0.0	13.321035617024995	0.0
34	3.6917760765526686E-4	0.0	0.0	13.577860172750508	0.0
35	3.6917760765526686E-4	0.0	0.0	13.87024883801348	0.0
36	4.922368102070225E-4	0.0	0.0	14.121904907231821	0.0
37	4.922368102070225E-4	0.0	0.0	14.384513245477267	0.0
38	6.152960127587781E-4	0.0	0.0	14.685023818108654	0.0
39	6.152960127587781E-4	0.0	0.0	14.993656298108457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	45	3.8380676E-10	45.000004	1
TCTAGCG	45	3.8380676E-10	45.000004	1
TACGACG	45	3.8380676E-10	45.000004	1
GATTAGC	45	3.8380676E-10	45.000004	23
ACCGGTT	20	7.032065E-4	45.0	24
TCACCGA	20	7.032065E-4	45.0	2
ATTAGCG	20	7.032065E-4	45.0	1
CGAAACG	20	7.032065E-4	45.0	1
TTCGTAA	20	7.032065E-4	45.0	30
ACGGGTC	165	0.0	45.0	5
AACGCAC	20	7.032065E-4	45.0	20
AATTCGG	25	3.889793E-5	45.0	2
GTATCGC	20	7.032065E-4	45.0	39
ATGCGAG	40	6.8102963E-9	45.0	1
TACTACG	75	0.0	45.0	1
TATCGAG	20	7.032065E-4	45.0	1
TACACCG	25	3.889793E-5	45.0	1
ACGACAT	20	7.032065E-4	45.0	36
CGAACCG	30	2.1647138E-6	44.999996	1
GCCGATG	100	0.0	42.75	9
>>END_MODULE