SRR3553187_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197225
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1960	0.9937888198757764	RNA PCR Primer, Index 38 (96% over 26bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1890	0.9582963620230702	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	1803	0.914184307263278	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1586	0.8041576879198884	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1379	0.6992014196983141	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	764	0.3873748257066802	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	578	0.29306629484091773	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	409	0.2073773608822411	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	403	0.204335150209152	RNA PCR Primer, Index 38 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	375	0.19013816706806946	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	340	0.17239193814171633	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	336	0.17036379769299026	RNA PCR Primer, Index 38 (96% over 25bp)
AAAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	325	0.16478641145899353	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	304	0.15413867410318166	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	290	0.1470401825326404	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	284	0.1439979718595513	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGTC	236	0.11966028647483837	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	229	0.11611104068956775	RNA PCR Primer, Index 27 (95% over 22bp)
GAAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	217	0.11002661934338953	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	215	0.10901254911902648	RNA PCR Primer, Index 38 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.0190616E-4	45.000004	43
AAATCGG	20	7.0190616E-4	45.000004	12
ACCGGAT	20	7.0190616E-4	45.000004	43
AATCATA	20	7.0190616E-4	45.000004	41
ATCTATT	20	7.0190616E-4	45.000004	32
CGTATGA	20	7.0190616E-4	45.000004	35
CTCGTCA	20	7.0190616E-4	45.000004	36
ATTAGCA	20	7.0190616E-4	45.000004	3
CTCAAGG	40	6.7648216E-9	45.000004	2
TACTTAA	20	7.0190616E-4	45.000004	12
CAACGTG	20	7.0190616E-4	45.000004	36
GGTCGCA	20	7.0190616E-4	45.000004	33
GTTTCAG	20	7.0190616E-4	45.000004	20
CGTGCGG	20	7.0190616E-4	45.000004	2
GCGCGAC	20	7.0190616E-4	45.000004	9
ATTACGT	20	7.0190616E-4	45.000004	12
ATTACGG	20	7.0190616E-4	45.000004	2
CCGGATA	20	7.0190616E-4	45.000004	44
CCAATGT	20	7.0190616E-4	45.000004	15
GCGCCTC	20	7.0190616E-4	45.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content