##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197225
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635036126251745	31.0	31.0	34.0	30.0	34.0
2	31.830777031309417	33.0	31.0	34.0	30.0	34.0
3	31.992607428064392	33.0	31.0	34.0	30.0	34.0
4	35.6811154772468	37.0	35.0	37.0	33.0	37.0
5	35.42536189631132	37.0	35.0	37.0	33.0	37.0
6	35.44811763214603	37.0	35.0	37.0	33.0	37.0
7	35.41622512358981	37.0	35.0	37.0	33.0	37.0
8	35.50944352896438	37.0	35.0	37.0	33.0	37.0
9	37.21058182279123	39.0	37.0	39.0	34.0	39.0
10	36.81774622892635	39.0	37.0	39.0	32.0	39.0
11	36.81247306375967	39.0	37.0	39.0	32.0	39.0
12	36.68953226010901	39.0	35.0	39.0	32.0	39.0
13	36.687676511598426	39.0	35.0	39.0	32.0	39.0
14	37.787161870959565	40.0	37.0	41.0	33.0	41.0
15	37.811925465838506	40.0	37.0	41.0	33.0	41.0
16	37.69486119913804	39.0	36.0	41.0	33.0	41.0
17	37.624286981873496	39.0	36.0	41.0	33.0	41.0
18	37.47249080998859	39.0	36.0	41.0	32.0	41.0
19	37.44458359741412	39.0	36.0	41.0	32.0	41.0
20	37.41004943592344	39.0	36.0	41.0	32.0	41.0
21	37.38041323361643	39.0	36.0	41.0	32.0	41.0
22	37.35725186969198	39.0	35.0	41.0	32.0	41.0
23	37.19043224743314	39.0	35.0	41.0	32.0	41.0
24	37.084401064773736	39.0	35.0	40.0	31.0	41.0
25	37.13400938014958	39.0	35.0	41.0	32.0	41.0
26	36.98860185067816	39.0	35.0	40.0	31.0	41.0
27	36.96479148181012	39.0	35.0	40.0	31.0	41.0
28	36.87805805552034	39.0	35.0	40.0	31.0	41.0
29	36.62870325770059	39.0	35.0	40.0	31.0	41.0
30	36.666015971606036	39.0	35.0	40.0	31.0	41.0
31	36.54482443909241	39.0	35.0	40.0	30.0	41.0
32	36.497005957662566	39.0	35.0	40.0	30.0	41.0
33	36.36391938141716	39.0	35.0	40.0	30.0	41.0
34	36.26476359487894	39.0	35.0	40.0	30.0	41.0
35	36.152566865255416	38.0	35.0	40.0	30.0	41.0
36	36.04476105970338	38.0	35.0	40.0	30.0	41.0
37	36.0313296995817	38.0	35.0	40.0	30.0	41.0
38	35.977857776651035	38.0	35.0	40.0	30.0	41.0
39	35.839021422233486	38.0	35.0	40.0	29.0	41.0
40	35.722646723285585	38.0	35.0	40.0	28.0	41.0
41	35.641921663075166	38.0	35.0	40.0	28.0	41.0
42	35.55064013182913	38.0	35.0	40.0	28.0	41.0
43	35.392556724553174	38.0	34.0	40.0	27.0	41.0
44	35.341513499809864	38.0	34.0	40.0	27.0	41.0
45	35.148373684877676	38.0	34.0	40.0	26.0	41.0
46	34.91765749778172	37.0	34.0	40.0	26.0	41.0
47	34.82158448472557	37.0	34.0	40.0	26.0	41.0
48	34.72598808467487	37.0	34.0	40.0	26.0	41.0
49	34.537031309418175	37.0	33.0	40.0	25.0	41.0
50	34.4379769299024	37.0	33.0	40.0	24.0	41.0
51	33.01980986183293	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	2.0
14	8.0
15	15.0
16	40.0
17	64.0
18	143.0
19	255.0
20	449.0
21	653.0
22	968.0
23	1182.0
24	1491.0
25	1602.0
26	1739.0
27	2066.0
28	2558.0
29	3110.0
30	4050.0
31	5315.0
32	6865.0
33	9162.0
34	13792.0
35	17462.0
36	19256.0
37	27138.0
38	39002.0
39	38827.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.99505640765623	36.12016732158702	19.64963873748257	25.235137533274177
2	22.309544935986818	35.08885790340981	18.658892128279884	23.942705032323488
3	19.384459373811637	32.244897959183675	23.118265939916338	25.25237672708835
4	22.227912282925594	31.856002028140452	18.843452909113957	27.072632779820005
5	16.86195969070858	37.33907973127139	17.757890733933323	28.041069844086703
6	19.737862847002155	36.999873241221955	21.593104322474332	21.66915958930156
7	76.30168589174801	10.287235391050832	8.032450247179618	5.378628470021549
8	77.43085308657625	6.966662441374065	6.51083787552288	9.09164659652681
9	72.48066928634807	9.925719356065407	11.203954873875016	6.389656483711496
10	35.2840664215997	30.849537330460137	14.447458486500189	19.41893776143998
11	25.719863100519714	27.896057802002787	25.7092153631639	20.6748637343136
12	24.466979338319177	23.184687539612117	32.77576372163772	19.572569400430982
13	25.594118392698697	24.76511598428191	27.687666370896185	21.95309925212321
14	19.440233236151602	27.43313474458106	30.43833185448092	22.688300164786412
15	17.458232982634048	29.52516161744201	28.850297883128405	24.166307516795538
16	19.588794524020788	27.261756876663707	28.262644188110027	24.886804411205475
17	19.9533527696793	28.005577386234	27.527950310559007	24.513119533527696
18	20.78793256433008	26.12092787425529	26.74001774622893	26.351121815185703
19	20.651540119153253	31.274432754468247	27.704398529598173	20.369628596780327
20	22.72075041196603	29.868931423501078	24.670300418303967	22.740017746228926
21	20.25047534541767	28.77120040562809	26.187856509063252	24.790467739890985
22	19.309418177208773	28.67739890987451	25.93940930409431	26.07377360882241
23	22.186842438838887	28.067942705032323	23.798707060463936	25.946507795664846
24	18.64723032069971	31.6597794397262	24.522246165546964	25.170744074027123
25	22.310051971099	26.45506401318291	24.164279376346812	27.070604639371275
26	22.820129293953606	28.97198631005197	23.940676891874762	24.26720750411966
27	19.70338445937381	26.748130308023832	26.047407782988973	27.501077449613387
28	23.86259348459881	26.095069083534035	25.68284953733046	24.359487894536695
29	22.77449613385727	30.895677525668653	24.502978831284068	21.82684750919001
30	26.784129800988723	25.8846495119787	22.84091773355305	24.490302953479528
31	24.167321587019902	29.290911395614145	20.13740651540119	26.404360501964764
32	25.687412853340092	25.810115350488022	23.70034224870072	24.802129547471164
33	25.433388262137154	28.388895931043223	23.205983014323742	22.97173279249588
34	22.92204335150209	25.349727468627204	27.447838762834326	24.28039041703638
35	27.442261376600328	27.49195081759412	22.956521739130434	22.10926606667512
36	24.69768031436177	27.090886043858536	27.66586386107238	20.545569780707314
37	25.326910888579036	27.474204588667767	23.203447838762834	23.995436683990366
38	24.297629610850553	27.378881987577643	26.191405754848525	22.132082646723287
39	23.058435796678918	24.555710482950943	28.42945874001775	23.95639498035239
40	25.335530485486117	26.232475598935228	24.94866269489162	23.48333122068703
41	22.78514387121308	22.478387628343263	30.11737862847002	24.619089871973635
42	27.63746989479021	23.902649258461146	24.37114970211687	24.088731144631765
43	22.1645328939029	23.674483457979463	27.638483965014576	26.522499683103057
44	24.498415515274434	20.726074280643935	25.65952592217011	29.115984281911523
45	26.39675497528204	23.36214982887565	25.95360628723539	24.28748890860692
46	21.182405881607302	23.5254151349981	33.25947521865889	22.032703764735707
47	23.290150842945874	24.401571808847763	25.744200785904425	26.564076562301942
48	22.302953479528455	22.24312333629104	32.21143364177969	23.24248954240081
49	24.529344657117505	20.63531499556344	29.29800988718469	25.537330460134367
50	22.156420332108	20.713905437951578	27.79059449866903	29.33907973127139
51	23.257700595766256	20.131829129167194	31.375839776904552	25.234630498161998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1639.0
1	1356.0
2	1073.0
3	751.5
4	430.0
5	405.0
6	380.0
7	380.0
8	380.0
9	380.0
10	380.0
11	414.5
12	449.0
13	459.0
14	469.0
15	539.5
16	610.0
17	598.0
18	586.0
19	594.0
20	602.0
21	610.0
22	618.0
23	805.5
24	993.0
25	1058.5
26	1222.5
27	1321.0
28	1499.0
29	1677.0
30	1745.0
31	1813.0
32	2400.0
33	2987.0
34	3295.5
35	3604.0
36	3923.5
37	4243.0
38	4369.0
39	4495.0
40	4869.0
41	5243.0
42	6746.5
43	8250.0
44	11965.0
45	15680.0
46	17360.5
47	19041.0
48	20151.5
49	21262.0
50	19925.5
51	18589.0
52	16971.5
53	15354.0
54	13399.0
55	11444.0
56	11019.5
57	10595.0
58	9692.0
59	8789.0
60	8782.0
61	8775.0
62	8047.0
63	7319.0
64	6325.0
65	5331.0
66	4571.0
67	3811.0
68	3127.5
69	2444.0
70	2232.0
71	2020.0
72	1621.0
73	1222.0
74	1058.5
75	731.5
76	568.0
77	416.0
78	264.0
79	261.5
80	259.0
81	168.5
82	78.0
83	59.5
84	41.0
85	42.0
86	43.0
87	27.5
88	12.0
89	12.0
90	12.0
91	9.5
92	7.0
93	4.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.920775763721636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83655741744701	25.95360628723539
2	9.163997042878265	6.033717834960071
3	3.4006899950714637	3.358600583090379
4	1.4015524889107935	1.8456078083407275
5	0.7978068013799902	1.3132209405501332
6	0.6422498767865944	1.2686018506781593
7	0.43740758994578616	1.007985803016859
8	0.35269837358304584	0.928888325516542
9	0.24796697880729424	0.7346938775510203
>10	3.825776244455397	30.25630624920776
>50	0.8008871365204534	17.589555076689063
>100	0.08162888122227697	4.659145645835975
>500	0.0030803351404632825	0.6804411205475979
>1k	0.007700837851158206	4.369628596780327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1960	0.9937888198757764	RNA PCR Primer, Index 38 (96% over 26bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1890	0.9582963620230702	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	1803	0.914184307263278	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1586	0.8041576879198884	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1379	0.6992014196983141	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	764	0.3873748257066802	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	578	0.29306629484091773	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	409	0.2073773608822411	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	403	0.204335150209152	RNA PCR Primer, Index 38 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	375	0.19013816706806946	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	340	0.17239193814171633	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	336	0.17036379769299026	RNA PCR Primer, Index 38 (96% over 25bp)
AAAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	325	0.16478641145899353	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	304	0.15413867410318166	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	290	0.1470401825326404	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	284	0.1439979718595513	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGTC	236	0.11966028647483837	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	229	0.11611104068956775	RNA PCR Primer, Index 27 (95% over 22bp)
GAAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	217	0.11002661934338953	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	215	0.10901254911902648	RNA PCR Primer, Index 38 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.070351121815186E-4	0.0	0.0	1.1317023703891496	0.0
2	5.070351121815186E-4	0.0	0.0	1.5692736722018	0.0
3	5.070351121815186E-4	0.0	0.0	2.2375459500570414	0.0
4	5.070351121815186E-4	0.0	0.0	5.250348586639625	0.0
5	5.070351121815186E-4	0.0	0.0	5.687412853340094	0.0
6	5.070351121815186E-4	0.0	0.0	7.703384459373812	0.0
7	5.070351121815186E-4	0.0	0.0	9.200659145645837	0.0
8	5.070351121815186E-4	0.0	0.0	10.345544428951705	0.0
9	5.070351121815186E-4	0.0	0.0	12.619596907085816	0.0
10	5.070351121815186E-4	0.0	0.0	14.847002154899227	0.0
11	5.070351121815186E-4	0.0	0.0	17.574344023323615	0.0
12	5.070351121815186E-4	0.0	0.0	18.608695652173914	0.0
13	5.070351121815186E-4	0.0	0.0	19.074153885156548	0.0
14	5.070351121815186E-4	0.0	0.0	19.667384966408925	0.0
15	5.070351121815186E-4	0.0	0.0	20.026365825833437	0.0
16	5.070351121815186E-4	0.0	0.0	20.779312967422992	0.0
17	5.070351121815186E-4	0.0	0.0	21.63214602611231	0.0
18	5.070351121815186E-4	0.0	0.0	23.11775890480416	0.0
19	5.070351121815186E-4	0.0	0.0	23.58220306756243	0.0
20	5.070351121815186E-4	0.0	0.0	24.16022309544936	0.0
21	5.070351121815186E-4	0.0	0.0	24.61401952085182	0.0
22	5.070351121815186E-4	0.0	0.0	25.08201292939536	0.0
23	5.070351121815186E-4	0.0	0.0	25.600709849157052	0.0
24	5.070351121815186E-4	0.0	0.0	25.982000253517555	0.0
25	5.070351121815186E-4	0.0	0.0	26.328812270249713	0.0
26	5.070351121815186E-4	0.0	0.0	26.659399163392067	0.0
27	5.070351121815186E-4	0.0	0.0	27.069083534034732	0.0
28	5.070351121815186E-4	0.0	0.0	27.43313474458106	0.0
29	5.070351121815186E-4	0.0	0.0	27.74648244390924	0.0
30	5.070351121815186E-4	0.0	0.0	28.208898466218784	0.0
31	5.070351121815186E-4	0.0	0.0	28.52072506021042	0.0
32	5.070351121815186E-4	0.0	0.0	28.816833565724426	0.0
33	5.070351121815186E-4	0.0	0.0	29.182405881607302	0.0
34	5.070351121815186E-4	0.0	0.0	29.46938775510204	0.0
35	5.070351121815186E-4	0.0	0.0	29.758397769045505	0.0
36	5.070351121815186E-4	0.0	0.0	30.056027379896058	0.0
37	5.070351121815186E-4	0.0	0.0	30.35162885029788	0.0
38	5.070351121815186E-4	0.0	0.0	30.695905691469136	0.0
39	5.070351121815186E-4	0.0	0.0	31.051844340220562	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.0190616E-4	45.000004	43
AAATCGG	20	7.0190616E-4	45.000004	12
ACCGGAT	20	7.0190616E-4	45.000004	43
AATCATA	20	7.0190616E-4	45.000004	41
ATCTATT	20	7.0190616E-4	45.000004	32
CGTATGA	20	7.0190616E-4	45.000004	35
CTCGTCA	20	7.0190616E-4	45.000004	36
ATTAGCA	20	7.0190616E-4	45.000004	3
CTCAAGG	40	6.7648216E-9	45.000004	2
TACTTAA	20	7.0190616E-4	45.000004	12
CAACGTG	20	7.0190616E-4	45.000004	36
GGTCGCA	20	7.0190616E-4	45.000004	33
GTTTCAG	20	7.0190616E-4	45.000004	20
CGTGCGG	20	7.0190616E-4	45.000004	2
GCGCGAC	20	7.0190616E-4	45.000004	9
ATTACGT	20	7.0190616E-4	45.000004	12
ATTACGG	20	7.0190616E-4	45.000004	2
CCGGATA	20	7.0190616E-4	45.000004	44
CCAATGT	20	7.0190616E-4	45.000004	15
GCGCCTC	20	7.0190616E-4	45.000004	15
>>END_MODULE