SRR3553186_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486477
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	2220	0.45634223200685753	TruSeq Adapter, Index 21 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	1267	0.26044396754625604	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1136	0.23351566466657211	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1084	0.22282656734028536	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	747	0.15355299428338853	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGT	707	0.1453306117247064	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	609	0.12518577445593523	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCA	20	7.029269E-4	45.000004	38
TATCACG	20	7.029269E-4	45.000004	1
AGGTACG	20	7.029269E-4	45.000004	23
CAAACGC	70	0.0	45.000004	34
CTGTACG	20	7.029269E-4	45.000004	32
TCACACG	20	7.029269E-4	45.000004	1
TTTCGCG	20	7.029269E-4	45.000004	1
GAGTACG	20	7.029269E-4	45.000004	1
TTCACGT	20	7.029269E-4	45.000004	29
TACGGTT	20	7.029269E-4	45.000004	33
ATGTCCG	40	6.8012014E-9	45.000004	11
CCGTCGA	20	7.029269E-4	45.000004	1
TCTACGG	70	0.0	45.000004	2
CACTACG	35	1.2099918E-7	45.000004	1
ACGTGCG	20	7.029269E-4	45.000004	1
TCGTCAA	20	7.029269E-4	45.000004	24
AGATTCG	20	7.029269E-4	45.000004	13
ACTAATG	20	7.029269E-4	45.000004	1
CGTAATG	20	7.029269E-4	45.000004	1
TTACGTG	20	7.029269E-4	45.000004	29