SRR3553185_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491620
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	2362	0.48045238192099593	TruSeq Adapter, Index 21 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	1317	0.26788983361132584	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1192	0.24246369146902078	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1120	0.2278182335950531	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGT	871	0.17716935844758147	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	753	0.1531670802652455	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	599	0.12184207314592571	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.0293434E-4	45.0	45
TTGATCG	20	7.0293434E-4	45.0	21
AGCGTAT	25	3.8875372E-5	45.0	29
CGGAAAT	20	7.0293434E-4	45.0	39
AACCGCT	25	3.8875372E-5	45.0	29
GATACGT	20	7.0293434E-4	45.0	44
AGATCGA	25	3.8875372E-5	45.0	16
CGAACAG	40	6.8012014E-9	45.0	1
ACGCATG	40	6.8012014E-9	45.0	1
TCACACG	25	3.8875372E-5	45.0	1
CGGCATT	25	3.8875372E-5	45.0	24
CGACCGA	25	3.8875372E-5	45.0	28
ATTAAGT	20	7.0293434E-4	45.0	43
ATTCGCG	25	3.8875372E-5	45.0	1
GCGTTGA	20	7.0293434E-4	45.0	18
ATGTCCG	20	7.0293434E-4	45.0	11
TCGATAG	25	3.8875372E-5	45.0	1
TATGAGT	25	3.8875372E-5	45.0	17
TTATACG	25	3.8875372E-5	45.0	1
TTCCCGA	20	7.0293434E-4	45.0	15