##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491620
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843236646190146	33.0	31.0	34.0	30.0	34.0
2	32.057137626622186	33.0	31.0	34.0	30.0	34.0
3	32.19375330539848	34.0	31.0	34.0	30.0	34.0
4	35.863742321305075	37.0	35.0	37.0	35.0	37.0
5	35.63131280257109	37.0	35.0	37.0	33.0	37.0
6	35.67122167527766	37.0	35.0	37.0	33.0	37.0
7	35.74331394166226	37.0	35.0	37.0	35.0	37.0
8	35.75714169480493	37.0	35.0	37.0	35.0	37.0
9	37.45409869411334	39.0	37.0	39.0	35.0	39.0
10	37.05176355721899	39.0	37.0	39.0	33.0	39.0
11	37.0363024287051	39.0	37.0	39.0	33.0	39.0
12	36.93994955453399	39.0	37.0	39.0	33.0	39.0
13	36.90539034213417	39.0	37.0	39.0	33.0	39.0
14	38.10940767259265	40.0	37.0	41.0	33.0	41.0
15	38.1467800333591	40.0	37.0	41.0	33.0	41.0
16	38.037472031243645	40.0	37.0	41.0	33.0	41.0
17	38.00313046662056	40.0	37.0	41.0	33.0	41.0
18	37.988576542858304	40.0	37.0	41.0	33.0	41.0
19	38.006216183230954	40.0	37.0	41.0	33.0	41.0
20	37.8991680566291	40.0	37.0	41.0	33.0	41.0
21	37.84376754403808	40.0	37.0	41.0	33.0	41.0
22	37.81397013953867	40.0	37.0	41.0	33.0	41.0
23	37.67967942719987	40.0	36.0	41.0	33.0	41.0
24	37.561305479842154	39.0	36.0	41.0	32.0	41.0
25	37.59633863553151	39.0	36.0	41.0	32.0	41.0
26	37.537274724380616	39.0	36.0	41.0	33.0	41.0
27	37.459855172694354	39.0	36.0	41.0	32.0	41.0
28	37.36397827590415	39.0	36.0	41.0	32.0	41.0
29	37.18102396159635	39.0	36.0	41.0	31.0	41.0
30	37.27688051747285	39.0	36.0	41.0	32.0	41.0
31	37.12525934664985	39.0	35.0	41.0	31.0	41.0
32	37.100958057035925	39.0	35.0	41.0	31.0	41.0
33	37.033070257515966	39.0	35.0	41.0	31.0	41.0
34	36.845622635368784	39.0	35.0	41.0	31.0	41.0
35	36.81374638948782	39.0	35.0	41.0	31.0	41.0
36	36.69006346365079	39.0	35.0	41.0	31.0	41.0
37	36.59901549977625	39.0	35.0	40.0	30.0	41.0
38	36.560203002318865	39.0	35.0	40.0	30.0	41.0
39	36.47391074407062	39.0	35.0	40.0	30.0	41.0
40	36.32738700622432	39.0	35.0	40.0	30.0	41.0
41	36.180019120458894	38.0	35.0	40.0	30.0	41.0
42	36.137608315365526	38.0	35.0	40.0	30.0	41.0
43	36.08496399658273	38.0	35.0	40.0	30.0	41.0
44	36.025190187543224	38.0	35.0	40.0	29.0	41.0
45	35.79111305479842	38.0	35.0	40.0	29.0	41.0
46	35.59870835197917	38.0	34.0	40.0	28.0	41.0
47	35.44327325983483	38.0	34.0	40.0	28.0	41.0
48	35.31965745901306	37.0	34.0	40.0	28.0	41.0
49	35.17685610837639	37.0	34.0	40.0	27.0	41.0
50	35.11287173019812	37.0	34.0	40.0	27.0	41.0
51	33.78346080305927	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	1.0
11	4.0
12	9.0
13	5.0
14	16.0
15	17.0
16	33.0
17	90.0
18	201.0
19	375.0
20	612.0
21	932.0
22	1427.0
23	1871.0
24	2238.0
25	2841.0
26	3343.0
27	4217.0
28	5302.0
29	6795.0
30	8794.0
31	11390.0
32	14763.0
33	20521.0
34	31530.0
35	39400.0
36	47793.0
37	68984.0
38	101924.0
39	116161.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.8842195191408	32.32110166388674	20.527643301737115	26.267035515235342
2	25.727391074407063	30.2603636955372	19.199788454497376	24.81245677555836
3	20.707456978967496	31.301818477686016	20.518896708840163	27.471827834506325
4	21.35104348887352	30.926935437939875	18.918677026972052	28.80334404621456
5	18.761848582238315	34.121272527561935	17.596110817297912	29.520768072901838
6	21.28208779138359	36.15475367153492	19.0972702493796	23.465888287701883
7	84.53317603026727	5.832553598307636	5.137707985842724	4.496562385582361
8	85.51503193523453	4.684715837435418	4.368618038322281	5.43163418900777
9	78.83731337211668	8.117448435783734	8.019812049957284	5.025426142142305
10	37.78100972295675	31.67853219966641	12.923192709816526	17.61726536756031
11	27.884748382897364	29.26345551442171	22.92258248240511	19.929213620275824
12	26.975102721614252	25.188153451853058	28.365404173955493	19.471339652577193
13	26.361824173141855	25.930596802408363	25.75363085309792	21.953948171351854
14	21.82335950530898	27.610349456897605	28.23176437085554	22.33452666693788
15	20.79675359017127	29.311460070786378	26.526382165086854	23.365404173955497
16	22.227126642528784	28.984378178267768	25.6466376469631	23.141857532240348
17	21.697449249420284	28.329604165819127	26.879500427159186	23.0934461576014
18	25.06793865180424	25.96660021968187	24.879174972539765	24.086286155974125
19	23.520401936454984	29.992880680200155	24.81225336642122	21.67446401692364
20	26.141938895895205	27.892681339245755	24.686139701395387	21.27924006346365
21	24.7782840405191	27.5558358081445	25.830316097799113	21.835564053537286
22	24.60233513689435	25.770513811480413	24.881819291322564	24.745331760302673
23	22.824335869167243	28.595459908059066	23.648346283715064	24.93185793905862
24	21.115902526341486	27.874984744314713	26.71738334485985	24.29172938448395
25	22.964891582929905	28.86273951425898	23.930067938651806	24.24230096415931
26	25.66413083275701	27.66201537773077	24.081200927545666	22.59265286196656
27	23.547658760831535	25.50974329766893	25.38078190472316	25.561816036776374
28	23.95549408079411	28.319840527236483	25.210935275212563	22.513730116756843
29	22.962857491558523	29.746755624262644	23.664415605549003	23.625971278629837
30	25.02461250559375	25.531711484479885	24.7703510841707	24.673324925755665
31	25.3132500711932	29.340954395671453	21.30527643301737	24.040519100117976
32	24.80086245474147	26.835767462674426	23.628412188275497	24.73495789430861
33	23.946747487897156	27.255807330865302	22.849965420446686	25.947479760790852
34	22.46226760506082	27.655709694479473	26.0355559171718	23.846466783287905
35	24.900126113665024	27.378056222285508	24.789064724787437	22.932752939262034
36	25.210121638664006	29.927382938041575	23.927220210731868	20.935275212562548
37	23.684959928399984	29.95443635328099	21.92730157438672	24.433302143932305
38	24.814287457792606	28.868434970098853	24.119035027053414	22.198242545055123
39	24.80981245677556	25.366950083397743	24.889752247670966	24.93348521215573
40	26.01460477604654	25.721085391155768	25.164964810219274	23.09934502257841
41	22.19478458972377	25.509133070257516	27.763313128025715	24.532769211993003
42	25.318945527033076	26.62442536918758	24.53439648509011	23.52223261868923
43	23.72198039135918	24.97904885887474	27.009682274927787	24.289288474838287
44	25.853301330295757	23.653228103006388	24.685936292258248	25.807534274439607
45	27.109556161262766	24.377364631219233	25.31141938895895	23.20165981855905
46	23.520808754729263	24.74431471461698	27.231194825271547	24.503681705382206
47	23.769781538586713	26.91326634392417	25.589276270290064	23.727675847199055
48	22.777144949351126	26.072576380130997	28.411781457223057	22.73849721329482
49	24.262641877873154	25.53374557585127	26.315040071600016	23.888572474675563
50	22.721410845775193	24.989829543143077	26.284528701029252	26.004230910052478
51	22.917700663113788	24.271795289044384	27.83511655343558	24.97538749440625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1402.0
1	1169.5
2	937.0
3	652.0
4	367.0
5	345.5
6	324.0
7	347.5
8	371.0
9	426.5
10	482.0
11	508.5
12	535.0
13	662.5
14	790.0
15	769.5
16	749.0
17	1005.5
18	1262.0
19	1127.0
20	992.0
21	1207.5
22	1423.0
23	1765.5
24	2108.0
25	2180.5
26	2826.0
27	3399.0
28	3985.5
29	4572.0
30	5852.0
31	7132.0
32	7820.5
33	8509.0
34	10280.0
35	12051.0
36	12451.5
37	12852.0
38	14438.5
39	16025.0
40	16990.0
41	17955.0
42	20070.5
43	22186.0
44	26719.5
45	31253.0
46	33454.5
47	35656.0
48	40144.0
49	44632.0
50	45108.0
51	45584.0
52	42000.5
53	38417.0
54	34728.0
55	31039.0
56	30273.0
57	29507.0
58	27070.5
59	24634.0
60	24061.5
61	23489.0
62	20626.0
63	17763.0
64	15729.5
65	13696.0
66	12480.0
67	11264.0
68	9895.5
69	8527.0
70	7381.5
71	6236.0
72	5437.5
73	4639.0
74	3835.0
75	2401.0
76	1771.0
77	1373.5
78	976.0
79	736.5
80	497.0
81	332.0
82	167.0
83	113.0
84	59.0
85	45.5
86	32.0
87	40.5
88	49.0
89	28.0
90	7.0
91	6.5
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.575265057733077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83791013623372	20.419878283526863
2	9.32521625656043	4.956401874775498
3	3.1827958960077467	2.5375093368321275
4	1.543273871722573	1.6405164879080534
5	0.8837399393258142	1.174281156484423
6	0.5677931043191025	0.9053551347139942
7	0.4706562174410112	0.8755469610695449
8	0.3844765952001982	0.8174053940752012
9	0.3037258904831299	0.7264436440036667
>10	4.902605981773673	33.042400506800426
>50	1.4747796538829518	26.452184859374565
>100	0.11764404955374955	4.773616970899021
>500	0.0023067460696813637	0.45425069672995705
>1k	0.0030756614262418182	1.2242086928066453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	2362	0.48045238192099593	TruSeq Adapter, Index 21 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	1317	0.26788983361132584	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1192	0.24246369146902078	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1120	0.2278182335950531	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGT	871	0.17716935844758147	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	753	0.1531670802652455	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	599	0.12184207314592571	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5581546723078801	0.0
2	0.0	0.0	0.0	0.7520035800008137	0.0
3	0.0	0.0	0.0	1.0034172735039257	0.0
4	0.0	0.0	0.0	2.32781416541231	0.0
5	0.0	0.0	0.0	2.5302062568650583	0.0
6	0.0	0.0	0.0	3.587933769984948	0.0
7	0.0	0.0	0.0	4.376957812944958	0.0
8	0.0	0.0	0.0	5.065090923884301	0.0
9	0.0	0.0	0.0	6.058744558805581	0.0
10	0.0	0.0	0.0	7.437248281192791	0.0
11	0.0	0.0	0.0	8.831821325413937	0.0
12	0.0	0.0	0.0	9.426793051543875	0.0
13	0.0	0.0	0.0	9.688580611041049	0.0
14	0.0	0.0	0.0	9.974980676131972	0.0
15	0.0	0.0	0.0	10.261990968634311	0.0
16	0.0	0.0	0.0	10.774785403360319	0.0
17	0.0	0.0	0.0	11.385826451324194	0.0
18	0.0	0.0	0.0	12.195191407998047	0.0
19	0.0	0.0	0.0	12.555428989870226	0.0
20	0.0	0.0	0.0	12.976689312883934	0.0
21	0.0	0.0	0.0	13.397542817623368	0.0
22	0.0	0.0	0.0	13.855416785321996	0.0
23	0.0	0.0	0.0	14.298035067735242	0.0
24	0.0	0.0	0.0	14.616166958219763	0.0
25	0.0	0.0	0.0	14.915381798950408	0.0
26	0.0	0.0	0.0	15.2111386843497	0.0
27	0.0	0.0	0.0	15.568935356576217	0.0
28	0.0	0.0	0.0	15.879337699849478	0.0
29	0.0	0.0	0.0	16.222488914202025	0.0
30	0.0	0.0	0.0	16.685651519466255	0.0
31	0.0	0.0	0.0	16.968593629225825	0.0
32	0.0	0.0	0.0	17.285301655750377	0.0
33	0.0	0.0	0.0	17.59529718074936	0.0
34	0.0	0.0	0.0	17.872543834669052	0.0
35	0.0	0.0	0.0	18.21589845815874	0.0
36	0.0	0.0	0.0	18.516740571986492	0.0
37	0.0	0.0	0.0	18.798055408648956	0.0
38	0.0	0.0	0.0	19.086896383385543	0.0
39	0.0	0.0	0.0	19.396485090110247	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.0293434E-4	45.0	45
TTGATCG	20	7.0293434E-4	45.0	21
AGCGTAT	25	3.8875372E-5	45.0	29
CGGAAAT	20	7.0293434E-4	45.0	39
AACCGCT	25	3.8875372E-5	45.0	29
GATACGT	20	7.0293434E-4	45.0	44
AGATCGA	25	3.8875372E-5	45.0	16
CGAACAG	40	6.8012014E-9	45.0	1
ACGCATG	40	6.8012014E-9	45.0	1
TCACACG	25	3.8875372E-5	45.0	1
CGGCATT	25	3.8875372E-5	45.0	24
CGACCGA	25	3.8875372E-5	45.0	28
ATTAAGT	20	7.0293434E-4	45.0	43
ATTCGCG	25	3.8875372E-5	45.0	1
GCGTTGA	20	7.0293434E-4	45.0	18
ATGTCCG	20	7.0293434E-4	45.0	11
TCGATAG	25	3.8875372E-5	45.0	1
TATGAGT	25	3.8875372E-5	45.0	17
TTATACG	25	3.8875372E-5	45.0	1
TTCCCGA	20	7.0293434E-4	45.0	15
>>END_MODULE