SRR3553183_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390957
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	1906	0.4875216456029691	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	959	0.24529551843297343	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	777	0.19874308427781062	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	697	0.1782804758579588	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	648	0.16574712820079956	RNA PCR Primer, Index 33 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	537	0.1373552590182552	RNA PCR Primer, Index 33 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	426	0.10896338983571083	Illumina PCR Primer Index 5 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTA	20	7.0275686E-4	45.000004	31
TCGTTTA	20	7.0275686E-4	45.000004	38
TGATAGT	30	2.1618125E-6	45.000004	32
TTGGCAC	20	7.0275686E-4	45.000004	31
CGAACCT	20	7.0275686E-4	45.000004	45
GATCGCC	30	2.1618125E-6	45.000004	36
TTTACGG	30	2.1618125E-6	45.000004	2
ACTTGCG	30	2.1618125E-6	45.000004	1
CCCTACG	20	7.0275686E-4	45.000004	31
GCGCGAT	20	7.0275686E-4	45.000004	12
TAGGTCG	20	7.0275686E-4	45.000004	1
CGGCACT	20	7.0275686E-4	45.000004	21
TCCTCGA	20	7.0275686E-4	45.000004	18
TAACGCG	20	7.0275686E-4	45.000004	1
CGACACG	20	7.0275686E-4	45.000004	1
CCCACCG	20	7.0275686E-4	45.000004	1
TCGTGCA	20	7.0275686E-4	45.000004	19
CGCTACG	40	6.7957444E-9	45.000004	1
TGCGACG	20	7.0275686E-4	45.000004	1
GTCCGTT	20	7.0275686E-4	45.000004	21