FastQCFastQC Report
Sat 18 Jun 2016
SRR3553182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300783
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT33770.2596128639442551No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT28760.2210976004452703No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC27540.21171863408424005No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT24430.18780995754095803Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG453.8562575E-1045.01
CCGATAG502.1827873E-1145.01
TAGCGCA253.891091E-545.024
TTACGCG950.042.631581
ATTAGCG900.042.51
CATACCG700.041.7857171
TACGCGG2200.040.9090922
CGTATAG501.0822987E-940.51
TTATGCG451.9286745E-840.01
CGTAACG750.039.01
TAGCACG1450.038.7931061
CGTAAGG1800.038.752
CAAACGG3150.038.571432
CTCGTAA356.2489235E-638.5714333
TTATACG356.2489235E-638.571431
AATTGCG700.038.571431
CGAATCG356.2489235E-638.571431
GTATACG356.2489235E-638.571431
TTACCGG2100.038.5714262
TATCGCG1000.038.251
Produced by FastQC (version 0.11.5)