##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1300783
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.991521260656082	33.0	31.0	34.0	30.0	34.0
2	32.18886163180177	34.0	31.0	34.0	30.0	34.0
3	32.34095002779095	34.0	31.0	34.0	30.0	34.0
4	35.96750034402356	37.0	35.0	37.0	35.0	37.0
5	35.738324532224055	37.0	35.0	37.0	35.0	37.0
6	35.09444388495237	37.0	35.0	37.0	33.0	37.0
7	35.487663968548176	37.0	35.0	37.0	35.0	37.0
8	35.883375628371525	37.0	35.0	37.0	35.0	37.0
9	37.5895472188674	39.0	37.0	39.0	35.0	39.0
10	37.15739827473145	39.0	37.0	39.0	34.0	39.0
11	37.10585777950665	39.0	37.0	39.0	33.0	39.0
12	36.93821259964191	39.0	37.0	39.0	33.0	39.0
13	36.93059026755424	39.0	37.0	39.0	33.0	39.0
14	38.16036494941893	40.0	37.0	41.0	33.0	41.0
15	38.16769207469655	40.0	37.0	41.0	33.0	41.0
16	38.042048520006794	40.0	37.0	41.0	33.0	41.0
17	38.02534857851002	40.0	37.0	41.0	33.0	41.0
18	37.99463784505179	40.0	37.0	41.0	33.0	41.0
19	38.05078479654178	40.0	37.0	41.0	33.0	41.0
20	37.968279105738624	40.0	37.0	41.0	33.0	41.0
21	37.980084303069766	40.0	37.0	41.0	33.0	41.0
22	37.9355749575448	40.0	37.0	41.0	33.0	41.0
23	37.86380972076049	40.0	37.0	41.0	33.0	41.0
24	37.72943911474858	40.0	36.0	41.0	33.0	41.0
25	37.78646861159778	40.0	36.0	41.0	33.0	41.0
26	37.751721078765634	40.0	36.0	41.0	33.0	41.0
27	37.66901397081604	40.0	36.0	41.0	33.0	41.0
28	37.63587777515543	40.0	36.0	41.0	33.0	41.0
29	37.430856645574245	39.0	36.0	41.0	32.0	41.0
30	37.46753455418774	40.0	36.0	41.0	32.0	41.0
31	37.440830638161785	40.0	36.0	41.0	32.0	41.0
32	37.40791277253777	39.0	36.0	41.0	32.0	41.0
33	37.172102495189435	39.0	35.0	41.0	32.0	41.0
34	37.11680349451061	39.0	35.0	41.0	31.0	41.0
35	37.06405219010396	39.0	35.0	41.0	31.0	41.0
36	37.05604778045223	39.0	35.0	41.0	31.0	41.0
37	37.01239714848672	39.0	35.0	41.0	31.0	41.0
38	36.860073509570775	39.0	35.0	41.0	31.0	41.0
39	36.82945964084709	39.0	35.0	41.0	31.0	41.0
40	36.73178001250017	39.0	35.0	41.0	31.0	41.0
41	36.681711707486954	39.0	35.0	40.0	31.0	41.0
42	36.62071229405673	39.0	35.0	40.0	31.0	41.0
43	36.5530207574976	39.0	35.0	40.0	31.0	41.0
44	36.462963461238346	38.0	35.0	40.0	31.0	41.0
45	36.2916182022674	38.0	35.0	40.0	30.0	41.0
46	36.17479856363436	38.0	35.0	40.0	30.0	41.0
47	36.03690238879198	38.0	35.0	40.0	30.0	41.0
48	35.93848474341993	38.0	35.0	40.0	30.0	41.0
49	35.825059983102484	38.0	35.0	40.0	30.0	41.0
50	35.738514417854475	37.0	34.0	40.0	29.0	41.0
51	34.55580139039333	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	9.0
11	15.0
12	30.0
13	38.0
14	46.0
15	76.0
16	120.0
17	206.0
18	409.0
19	650.0
20	1192.0
21	1918.0
22	2605.0
23	3655.0
24	4830.0
25	5989.0
26	7575.0
27	9328.0
28	11902.0
29	15604.0
30	20631.0
31	27689.0
32	36895.0
33	52552.0
34	87897.0
35	100351.0
36	117528.0
37	173193.0
38	267105.0
39	350654.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.668474295866414	30.806368164405594	20.947229476399983	27.57792806332801
2	23.121458383143075	30.072656238588603	20.56891887424728	26.23696650402104
3	21.07200047971107	29.876927973382184	22.100227324619095	26.95084422228765
4	21.39065470566574	30.64215937631411	19.77178361033316	28.195402307686983
5	19.79822922040033	33.03371892160337	17.63068859294748	29.53736326504882
6	20.02447756466682	37.31713898474995	19.291303776263987	23.36707967431924
7	86.96046919432374	4.879599441259611	4.336695667148172	3.8232356972684913
8	88.53851872295378	3.5300276833261193	3.5656985062074154	4.365755087512675
9	82.32395411071639	6.793139209230133	6.175280581003903	4.707626099049572
10	40.77751631132941	31.77632241503771	12.456112971956122	14.990048301676758
11	29.476553737249027	26.422162651264664	24.313894016142584	19.787389595343726
12	29.42942827512352	22.45424486636126	28.16242217187648	19.95390468663874
13	27.07238640111379	22.794732095976038	26.615046475853386	23.51783502705678
14	22.31871111476703	24.226562001502174	29.11869235683431	24.33603452689649
15	22.803265417829106	27.011576873314	28.03250042474417	22.152657284112724
16	25.43737118335649	25.33827702237806	26.825535081562414	22.39881671270304
17	24.749016553875627	26.16362606214872	26.70068720147788	22.386670182497774
18	25.393013285075224	26.207830206883088	25.674766659773386	22.724389848268313
19	24.786686172866652	29.410670342401463	24.37478042071583	21.42786306401606
20	26.904795034990464	27.488981636445125	24.722340313488107	20.883883015076304
21	27.42717271059047	27.088453646765064	24.60371945205311	20.880654190591358
22	26.237427764661746	25.09150257960013	26.378112260077202	22.29295739566092
23	25.80730221720302	25.805072790772943	25.149775173876044	23.237849818147993
24	24.735101857880984	25.46581558953338	26.7058379452991	23.093244607286532
25	24.616250366125634	25.654086807715043	23.91328914968907	25.81637367647025
26	24.87732388876546	26.435769840165502	24.362403260190206	24.324503010878832
27	23.363312712420132	26.16923806661065	25.277698124898617	25.189751096070594
28	23.42550602214205	26.968833387275204	26.11611621615596	23.48954437442679
29	25.035151904660502	26.841602327213685	24.21864369383671	23.9046020742891
30	25.71351255359272	24.596031774708003	26.410400504926645	23.280055166772627
31	26.358354929300276	25.22957326471825	24.88885540478312	23.523216401198354
32	26.84198671108094	24.899618153066267	24.628781280198158	23.629613855654632
33	26.529175119908547	24.975264898142118	25.961824531839667	22.533735450109667
34	24.20841908296772	25.34196710750371	27.73152785668324	22.718085952845325
35	24.748401539688018	25.977891777490942	27.496054299602623	21.777652383218417
36	25.70943808459981	28.71339800720028	25.239105984626182	20.338057923573725
37	24.931214506954657	28.5243580212841	24.47325956750665	22.07116790425459
38	25.657853769614146	26.692768893812417	25.632484434375296	22.016892902198137
39	25.123329563808877	26.294316577015536	24.174823932969602	24.407529926205985
40	25.899323715023954	25.915083453581424	24.667757804337846	23.51783502705678
41	22.950715069308256	25.943220352664508	27.15433704161263	23.951727536414605
42	23.87139130815824	26.87219928304721	24.622093000907917	24.634316407886633
43	23.182806048357026	25.224038137029776	26.4610622986309	25.132093515982294
44	24.01192205002679	24.545139350683396	26.11088859556129	25.332050003728522
45	25.149852050649496	24.97903186004122	26.264642142463423	23.60647394684586
46	22.92219378635791	25.655701219957518	27.013421915876823	24.40868307780775
47	23.61669855771485	25.247101169065093	26.787327325157236	24.348872948062823
48	23.42842733953319	24.30420754268775	28.92465538064381	23.342709737135248
49	24.23309652724551	24.78822370833567	27.039021881436025	23.939657882982786
50	22.75713935375847	24.35379306156369	27.37912472718355	25.509942857494295
51	22.764750154330123	23.671050436544757	28.527125585128342	25.037073823996774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1230.0
1	1015.0
2	800.0
3	627.5
4	455.0
5	503.0
6	551.0
7	582.5
8	614.0
9	711.0
10	808.0
11	889.0
12	970.0
13	1038.5
14	1107.0
15	1175.0
16	1243.0
17	1523.0
18	1803.0
19	1851.0
20	1899.0
21	2272.0
22	2645.0
23	3724.0
24	4803.0
25	5898.5
26	9318.0
27	11642.0
28	13710.0
29	15778.0
30	18531.5
31	21285.0
32	25537.0
33	29789.0
34	32215.0
35	34641.0
36	37964.0
37	41287.0
38	42678.0
39	44069.0
40	47323.5
41	50578.0
42	54107.0
43	57636.0
44	66926.5
45	76217.0
46	82460.0
47	88703.0
48	93718.5
49	98734.0
50	97434.0
51	96134.0
52	92406.0
53	88678.0
54	84802.0
55	80926.0
56	79039.5
57	77153.0
58	76113.0
59	75073.0
60	71615.0
61	68157.0
62	64891.0
63	61625.0
64	54912.0
65	48199.0
66	41283.5
67	34368.0
68	29259.0
69	24150.0
70	20372.5
71	16595.0
72	14336.0
73	12077.0
74	10121.5
75	6744.0
76	5322.0
77	4392.5
78	3463.0
79	2800.5
80	2138.0
81	1585.5
82	1033.0
83	871.0
84	709.0
85	487.0
86	265.0
87	191.0
88	117.0
89	90.0
90	63.0
91	47.0
92	31.0
93	26.5
94	22.0
95	17.5
96	13.0
97	12.5
98	12.0
99	12.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1300783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.390317948678454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41297298104645	17.405430975330958
2	8.776907103901092	4.105892955325222
3	3.0942215585629618	2.171244781753292
4	1.5679403263080371	1.4669849102759853
5	1.024828780780242	1.1985535512703176
6	0.7688792083060776	1.0790597487844396
7	0.6209549565445263	1.0167033705769
8	0.5238233402022144	0.9801915581014845
9	0.45997040826045626	0.9682968686575956
>10	7.070879780783533	39.942786605837554
>50	1.5584563556055335	24.45202268847216
>100	0.11513034496283525	3.695837895219139
>500	0.0033565698239787145	0.5202939569944866
>1k	0.0016782849119893572	0.9967001334004839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	3377	0.2596128639442551	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT	2876	0.2210976004452703	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	2754	0.21171863408424005	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	2443	0.18780995754095803	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2117955108576911	0.0
2	0.0	0.0	0.0	0.3280331923157052	0.0
3	0.0	0.0	0.0	0.5113842969965013	0.0
4	0.0	0.0	0.0	1.238331066749796	0.0
5	0.0	0.0	0.0	1.3777086570165815	0.0
6	0.0	0.0	0.0	2.072059674826624	0.0
7	7.687677345106754E-5	0.0	0.0	2.590209127886819	0.0
8	7.687677345106754E-5	0.0	0.0	3.093136979803703	0.0
9	7.687677345106754E-5	0.0	0.0	3.833075924270228	0.0
10	7.687677345106754E-5	0.0	0.0	4.623446032120653	0.0
11	7.687677345106754E-5	0.0	0.0	5.464708563995686	0.0
12	7.687677345106754E-5	0.0	0.0	5.791434851162723	0.0
13	7.687677345106754E-5	0.0	0.0	5.961486274036484	0.0
14	7.687677345106754E-5	0.0	0.0	6.160135856634042	0.0
15	7.687677345106754E-5	0.0	0.0	6.297130266923845	0.0
16	7.687677345106754E-5	0.0	0.0	6.530451274347835	0.0
17	7.687677345106754E-5	0.0	0.0	6.813972814835372	7.687677345106754E-5
18	7.687677345106754E-5	0.0	0.0	7.185672014471284	7.687677345106754E-5
19	7.687677345106754E-5	0.0	0.0	7.3839372132015875	7.687677345106754E-5
20	7.687677345106754E-5	0.0	0.0	7.595040833098219	7.687677345106754E-5
21	7.687677345106754E-5	0.0	0.0	7.843814071985873	7.687677345106754E-5
22	7.687677345106754E-5	0.0	0.0	8.105579485586759	7.687677345106754E-5
23	7.687677345106754E-5	0.0	0.0	8.355121492208923	7.687677345106754E-5
24	7.687677345106754E-5	0.0	0.0	8.574220296544466	7.687677345106754E-5
25	7.687677345106754E-5	0.0	0.0	8.761492116671267	7.687677345106754E-5
26	7.687677345106754E-5	0.0	0.0	8.942690671695432	7.687677345106754E-5
27	7.687677345106754E-5	0.0	0.0	9.129578107954979	7.687677345106754E-5
28	7.687677345106754E-5	0.0	0.0	9.315389269386209	7.687677345106754E-5
29	7.687677345106754E-5	0.0	0.0	9.515499510679337	7.687677345106754E-5
30	7.687677345106754E-5	0.0	0.0	9.807093112379237	7.687677345106754E-5
31	7.687677345106754E-5	0.0	0.0	10.009970917516604	7.687677345106754E-5
32	7.687677345106754E-5	0.0	0.0	10.23076101086807	7.687677345106754E-5
33	7.687677345106754E-5	0.0	0.0	10.441326493350543	7.687677345106754E-5
34	1.537535469021351E-4	0.0	0.0	10.650969454551605	7.687677345106754E-5
35	1.537535469021351E-4	0.0	0.0	10.8737583440128	7.687677345106754E-5
36	1.537535469021351E-4	0.0	0.0	11.100929209560704	7.687677345106754E-5
37	1.537535469021351E-4	0.0	0.0	11.32241119387323	7.687677345106754E-5
38	1.537535469021351E-4	0.0	0.0	11.599936346031582	7.687677345106754E-5
39	1.537535469021351E-4	0.0	0.0	11.882381611690805	7.687677345106754E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	45	3.8562575E-10	45.0	1
CCGATAG	50	2.1827873E-11	45.0	1
TAGCGCA	25	3.891091E-5	45.0	24
TTACGCG	95	0.0	42.63158	1
ATTAGCG	90	0.0	42.5	1
CATACCG	70	0.0	41.785717	1
TACGCGG	220	0.0	40.909092	2
CGTATAG	50	1.0822987E-9	40.5	1
TTATGCG	45	1.9286745E-8	40.0	1
CGTAACG	75	0.0	39.0	1
TAGCACG	145	0.0	38.793106	1
CGTAAGG	180	0.0	38.75	2
CAAACGG	315	0.0	38.57143	2
CTCGTAA	35	6.2489235E-6	38.57143	33
TTATACG	35	6.2489235E-6	38.57143	1
AATTGCG	70	0.0	38.57143	1
CGAATCG	35	6.2489235E-6	38.57143	1
GTATACG	35	6.2489235E-6	38.57143	1
TTACCGG	210	0.0	38.571426	2
TATCGCG	100	0.0	38.25	1
>>END_MODULE