FastQCFastQC Report
Sat 18 Jun 2016
SRR3553177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186582
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT18030.9663311573463679TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11990.642612899422238No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC6280.336581235060188No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT5740.3076395365040572No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT5400.2894169855613081No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT5350.2867371986579627TruSeq Adapter, Index 15 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT3990.2138469948869666No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.18222550942749033No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC3230.1731142339561158TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT2530.13559721730927957No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.13077360088325776No Hit
ACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG2120.1136229647018469No Hit
CTTGCTGGGAACTGGAAGGCTGGCGAGATGGCTTTGTGGGTAAAATGACTG2120.1136229647018469No Hit
AGCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT2100.11255104994050873TruSeq Adapter, Index 15 (95% over 21bp)
CCCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATC2070.11094317779850146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG453.8198777E-1045.0000042
AGTACGG453.8198777E-1045.0000042
TCACGCC253.87821E-545.043
TGATCAG253.87821E-545.01
AGCGTTG253.87821E-545.020
TTTAGCG253.87821E-545.017
CGGGTAT253.87821E-545.06
TCCATAT207.018083E-445.022
GCGACTA253.87821E-545.016
ATTAGTC207.018083E-445.018
GCGACGG207.018083E-445.02
TCACCGG207.018083E-445.02
CTCGTAC253.87821E-545.029
GGGCTCG253.87821E-545.07
CGAGTAG253.87821E-545.01
CCGATTG207.018083E-445.045
GAGTCTT253.87821E-545.09
AACCCTA253.87821E-545.042
TTAGCGT253.87821E-545.018
ACGTTAG207.018083E-445.031
Produced by FastQC (version 0.11.5)