##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186582
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.571721816681137	31.0	31.0	34.0	30.0	34.0
2	31.784464739363926	33.0	31.0	34.0	30.0	34.0
3	31.94145201573571	33.0	31.0	34.0	30.0	34.0
4	35.668124470742086	37.0	35.0	37.0	33.0	37.0
5	35.41457911266896	37.0	35.0	37.0	33.0	37.0
6	35.431418893569585	37.0	35.0	37.0	33.0	37.0
7	35.55629696326548	37.0	35.0	37.0	33.0	37.0
8	35.592447288591615	37.0	35.0	37.0	35.0	37.0
9	37.25718450868787	39.0	37.0	39.0	34.0	39.0
10	36.818980394679016	39.0	37.0	39.0	32.0	39.0
11	36.84248748539516	39.0	37.0	39.0	32.0	39.0
12	36.9250999560515	39.0	37.0	39.0	33.0	39.0
13	36.87895938514969	39.0	37.0	39.0	33.0	39.0
14	37.999040636288605	40.0	37.0	41.0	33.0	41.0
15	38.0672036959621	40.0	37.0	41.0	33.0	41.0
16	37.912628227803324	40.0	37.0	41.0	33.0	41.0
17	37.8357129841035	39.0	37.0	41.0	33.0	41.0
18	37.7494399245372	39.0	37.0	41.0	33.0	41.0
19	37.739717657651866	39.0	36.0	41.0	33.0	41.0
20	37.66058355039607	39.0	36.0	41.0	32.0	41.0
21	37.6219946189879	39.0	36.0	41.0	32.0	41.0
22	37.58689477012788	39.0	36.0	41.0	32.0	41.0
23	37.40004930807902	39.0	36.0	41.0	32.0	41.0
24	37.27780814869602	39.0	36.0	41.0	32.0	41.0
25	37.30319644981831	39.0	36.0	41.0	32.0	41.0
26	37.206316793688565	39.0	36.0	41.0	32.0	41.0
27	37.170488042790836	39.0	36.0	41.0	31.0	41.0
28	37.05587891650856	39.0	36.0	41.0	31.0	41.0
29	36.76821987115584	39.0	35.0	40.0	31.0	41.0
30	36.856577804932954	39.0	35.0	40.0	31.0	41.0
31	36.687761949169804	39.0	35.0	40.0	30.0	41.0
32	36.59802124535057	39.0	35.0	40.0	30.0	41.0
33	36.56946007653471	39.0	35.0	40.0	30.0	41.0
34	36.372688683795865	39.0	35.0	40.0	30.0	41.0
35	36.25082805415313	39.0	35.0	40.0	30.0	41.0
36	36.12834035437502	39.0	35.0	40.0	30.0	41.0
37	36.03913024836265	39.0	35.0	40.0	29.0	41.0
38	36.05152158300372	39.0	35.0	40.0	29.0	41.0
39	35.921010601236986	39.0	35.0	40.0	28.0	41.0
40	35.81978969032382	38.0	35.0	40.0	28.0	41.0
41	35.72488235735494	38.0	35.0	40.0	28.0	41.0
42	35.632199247515835	38.0	35.0	40.0	27.0	41.0
43	35.49386328799134	38.0	35.0	40.0	26.0	41.0
44	35.504427007964324	38.0	35.0	40.0	26.0	41.0
45	35.256884372554694	38.0	34.0	40.0	26.0	41.0
46	35.01287369628367	38.0	34.0	40.0	25.0	41.0
47	34.917296416588954	38.0	34.0	40.0	24.0	41.0
48	34.816986633222925	38.0	34.0	40.0	24.0	41.0
49	34.66320974156135	38.0	33.0	40.0	24.0	41.0
50	34.58992292932866	38.0	33.0	40.0	23.0	41.0
51	33.13730156177981	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	2.0
13	3.0
14	3.0
15	11.0
16	21.0
17	65.0
18	117.0
19	215.0
20	388.0
21	588.0
22	822.0
23	1176.0
24	1350.0
25	1572.0
26	1710.0
27	2007.0
28	2544.0
29	3022.0
30	3906.0
31	4815.0
32	6156.0
33	8048.0
34	11269.0
35	14916.0
36	17685.0
37	25346.0
38	37810.0
39	41006.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.752709264559282	34.25250024118082	20.002465403951078	25.99232509030882
2	21.57925201787954	33.08840081036756	20.590410650545067	24.741936521207833
3	19.648197575328812	33.23310930314821	20.853565724453592	26.26512739706939
4	19.884554780203878	34.24392492309011	19.10688062085303	26.764639675852976
5	17.45345210148889	35.53558221050262	18.709200244396566	28.301765443611927
6	19.268203792434427	38.44315100063243	19.8234556388076	22.465189568125542
7	80.17600840381172	7.878573495835611	6.8709736201777245	5.074444480174937
8	81.4805286683603	6.757350655475877	5.032639804482748	6.729480871681084
9	74.17864531412462	10.765775905499995	10.298421069556548	4.757157710818836
10	28.937946854466134	40.74830369489018	13.802510424371054	16.51123902627263
11	19.64766161794814	29.577879966985027	33.58844904653182	17.186009368535014
12	23.323257334576756	24.205443183158078	33.66937861101285	18.801920871252317
13	24.969182450611527	26.55240055310802	28.318916079793333	20.159500916487122
14	18.23594987726576	30.381816037988656	32.16762603037807	19.214608054367517
15	18.001736501913367	31.078024675477806	31.106430416653268	19.81380840595556
16	18.0531884104576	32.37986515312302	28.265320341726426	21.30162609469295
17	18.078378407349046	31.053906593347698	28.233698856266948	22.634016143036305
18	20.50894512868337	28.71391666934645	27.461384270722792	23.31575393124739
19	19.426311219731808	33.12966952867908	29.185559164335253	18.258460087253862
20	20.592554480067747	30.73501195184959	27.205196642762967	21.4672369253197
21	20.20612920860533	32.42863727476391	26.559367999056715	20.805865517574045
22	18.522151118543054	30.12509245264817	27.430834700024654	23.921921728784127
23	19.731270969332517	31.359402300329076	24.494324211338714	24.41500251899969
24	16.225037784995337	32.68589681748507	26.57919842214147	24.509866975378117
25	19.635334598192753	32.23998027676839	24.618130366273274	23.50655475876558
26	20.96022124320674	31.45105101242349	26.405548230804687	21.183179513565083
27	19.28374655647383	29.897310565863805	25.86101553204489	24.957927345617474
28	23.2444715996184	27.915876129530183	27.558928514004567	21.280723756846857
29	21.074916122669926	30.940819586026517	26.894341361974895	21.08992292932866
30	26.350880577976437	29.408517434693593	23.057422473764884	21.183179513565083
31	21.50046628292118	30.571008993364845	24.600443772711195	23.32808095100278
32	23.693067927238427	31.048011062160334	23.37846094478567	21.880460065815566
33	24.43054528303909	27.90354910977479	24.977757768702233	22.688147838483882
34	21.775412419204425	27.02725879238083	28.027355264709353	23.169973523705394
35	24.058590860854746	28.73857070885723	25.57374237600626	21.629096054281764
36	23.60302708728602	27.3675917291057	29.559121458661608	19.470259724946672
37	21.756117953500336	30.700710679486765	24.68994865528293	22.853222711729963
38	23.38650030549571	29.06979237011073	26.697645003269336	20.846062321124222
39	23.494763696390862	27.40028512932652	27.029938579284174	22.075012594998444
40	23.78954025575886	28.801813679776185	26.3498086632151	21.058837401249853
41	21.424360334866172	25.960703604849343	29.914997159425884	22.699938900858605
42	25.50406791651928	27.21537983299568	26.033057851239672	21.247494399245372
43	20.394786206600852	26.91577965720166	29.742418882850437	22.947015253347054
44	24.52594569679819	24.630457386028663	26.088261461448585	24.755335455724563
45	25.292364751154988	25.79080511517724	26.970983267410574	21.945846866257195
46	20.94735826607068	26.877726683174153	29.84371482779689	22.33120022295827
47	22.139327480678737	25.847080640147496	26.937217952428423	25.076373926745344
48	20.65311766408335	25.26878262640555	31.931804782883667	22.146294926627437
49	21.908329849610357	24.781061409996678	28.655497314853523	24.65511142553944
50	20.732975313803045	23.810442593604954	27.607700635645454	27.848881456946543
51	20.83748700303352	23.824913442883023	31.314917837733542	24.02268171634992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1465.0
1	1183.0
2	901.0
3	607.5
4	314.0
5	328.0
6	342.0
7	360.0
8	378.0
9	431.0
10	484.0
11	500.0
12	516.0
13	563.5
14	611.0
15	619.0
16	627.0
17	645.5
18	664.0
19	782.5
20	901.0
21	829.5
22	758.0
23	889.5
24	1021.0
25	1091.5
26	1254.5
27	1347.0
28	1977.0
29	2607.0
30	2978.5
31	3350.0
32	4012.5
33	4675.0
34	4893.5
35	5112.0
36	5541.5
37	5971.0
38	6162.0
39	6353.0
40	7154.0
41	7955.0
42	9110.0
43	10265.0
44	12717.0
45	15169.0
46	17125.5
47	19082.0
48	20715.0
49	22348.0
50	20824.0
51	19300.0
52	17256.0
53	15212.0
54	12887.5
55	10563.0
56	9158.5
57	7754.0
58	6909.0
59	6064.0
60	5269.5
61	4475.0
62	3994.5
63	3514.0
64	2773.0
65	2032.0
66	1556.0
67	1080.0
68	945.5
69	811.0
70	685.5
71	560.0
72	424.0
73	288.0
74	253.0
75	168.0
76	118.0
77	114.5
78	111.0
79	71.5
80	32.0
81	32.5
82	33.0
83	27.0
84	21.0
85	15.0
86	9.0
87	6.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	186582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.862269672315655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40159977934078	30.468641133657055
2	9.369742104537305	7.282588888531584
3	3.3747069369742104	3.934463131491784
4	1.681147427940974	2.613328188142479
5	1.0191697696869397	1.9803625215722844
6	0.7364501448076127	1.7172074476637618
7	0.47717556199144945	1.298088775980534
8	0.40546131568059574	1.2605717593336978
9	0.3723624327678941	1.3023764350258866
>10	3.7029375258585024	32.02988498354611
>50	0.39304923458833263	9.770503049597496
>100	0.057923045097227975	3.5126646729052107
>500	0.005516480485450282	1.2203749557835162
>1k	0.002758240242725141	1.6089440567686057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	1803	0.9663311573463679	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1199	0.642612899422238	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	628	0.336581235060188	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGT	574	0.3076395365040572	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	540	0.2894169855613081	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	535	0.2867371986579627	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	399	0.2138469948869666	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	340	0.18222550942749033	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	323	0.1731142339561158	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	253	0.13559721730927957	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	244	0.13077360088325776	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	212	0.1136229647018469	No Hit
CTTGCTGGGAACTGGAAGGCTGGCGAGATGGCTTTGTGGGTAAAATGACTG	212	0.1136229647018469	No Hit
AGCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	210	0.11255104994050873	TruSeq Adapter, Index 15 (95% over 21bp)
CCCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATC	207	0.11094317779850146	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.136765604399138	0.0
2	0.0	0.0	0.0	1.561779807269726	0.0
3	0.0	0.0	0.0	2.1315025029209678	0.0
4	0.0	0.0	0.0	4.772700474858239	0.0
5	0.0	0.0	0.0	5.148406598707271	0.0
6	0.0	0.0	0.0	7.135736566228253	0.0
7	0.0	0.0	0.0	8.701268075162663	0.0
8	0.0	0.0	0.0	10.26197596767105	0.0
9	0.0	0.0	0.0	12.111564888360078	0.0
10	0.0	0.0	0.0	14.74043584054196	0.0
11	0.0	0.0	0.0	17.01128726243689	0.0
12	0.0	0.0	0.0	18.167883289920784	0.0
13	0.0	0.0	0.0	18.695801309879837	0.0
14	0.0	0.0	0.0	19.203888906754134	0.0
15	0.0	0.0	0.0	19.61175247344331	0.0
16	0.0	0.0	0.0	20.445702157764416	0.0
17	0.0	0.0	0.0	21.47795607293308	0.0
18	0.0	0.0	0.0	23.12012948730317	0.0
19	0.0	0.0	0.0	23.681276864863705	0.0
20	0.0	0.0	0.0	24.478245489918642	0.0
21	0.0	0.0	0.0	25.164270937175075	0.0
22	0.0	0.0	0.0	25.892637017504367	0.0
23	0.0	0.0	0.0	26.560439913818055	0.0
24	0.0	0.0	0.0	27.048697087607593	0.0
25	0.0	0.0	0.0	27.43941001811536	0.0
26	0.0	0.0	0.0	27.815116141964392	0.0
27	0.0	0.0	0.0	28.395022027848345	0.0
28	0.0	0.0	0.0	28.86291282117246	0.0
29	0.0	0.0	0.0	29.31579680783784	0.0
30	0.0	0.0	0.0	30.060241609587205	0.0
31	0.0	0.0	0.0	30.519021127439945	0.0
32	0.0	0.0	0.0	30.934924054839158	0.0
33	0.0	0.0	0.0	31.361010172471087	0.0
34	0.0	0.0	0.0	31.799959267239068	0.0
35	0.0	0.0	0.0	32.216934109399624	0.0
36	0.0	0.0	0.0	32.62158193180478	0.0
37	0.0	0.0	0.0	33.02462188206794	0.0
38	0.0	0.0	0.0	33.44695629803518	0.0
39	0.0	0.0	0.0	33.866610927099075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	45	3.8198777E-10	45.000004	2
AGTACGG	45	3.8198777E-10	45.000004	2
TCACGCC	25	3.87821E-5	45.0	43
TGATCAG	25	3.87821E-5	45.0	1
AGCGTTG	25	3.87821E-5	45.0	20
TTTAGCG	25	3.87821E-5	45.0	17
CGGGTAT	25	3.87821E-5	45.0	6
TCCATAT	20	7.018083E-4	45.0	22
GCGACTA	25	3.87821E-5	45.0	16
ATTAGTC	20	7.018083E-4	45.0	18
GCGACGG	20	7.018083E-4	45.0	2
TCACCGG	20	7.018083E-4	45.0	2
CTCGTAC	25	3.87821E-5	45.0	29
GGGCTCG	25	3.87821E-5	45.0	7
CGAGTAG	25	3.87821E-5	45.0	1
CCGATTG	20	7.018083E-4	45.0	45
GAGTCTT	25	3.87821E-5	45.0	9
AACCCTA	25	3.87821E-5	45.0	42
TTAGCGT	25	3.87821E-5	45.0	18
ACGTTAG	20	7.018083E-4	45.0	31
>>END_MODULE